{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:56:49Z","timestamp":1792292209069,"version":"4.4.0"},"reference-count":21,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T00:00:00Z","timestamp":1695168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea (NRF)","award":["2022R1I1A1A01069499"],"award-info":[{"award-number":["2022R1I1A1A01069499"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Histological and naked-eye dissections are frequently used to investigate human anatomy. However, limitations of conventional methods include tissue damage and difficulty in observing structures, rendering findings limited. Micro-computed tomography (micro-CT) allows for a three-dimensional observation with whole-mount staining for contrast enhancement. A precise anatomical understanding of the larynx is essential for both the medical and surgical fields; however, the larynx is difficult to dissect because of its minuscule and complex structures. Therefore, we aimed to clarify the detailed anatomy of the larynx using micro-CT. The study was conducted on twelve specimens of cadavers using Lugol-based-contrast micro-CT. Using Lugol-micro-CT, relevant information on human structures was obtained. Consequently, we successfully employed the Lugol-micro-CT technique in the analysis of specific human soft tissue structures that are challenging to analyze using conventional methods.<\/jats:p>","DOI":"10.3390\/diagnostics13183005","type":"journal-article","created":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T21:47:03Z","timestamp":1695246423000},"page":"3005","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Observation of Anatomical Structures in the Human Larynx Using Micro-Computed Tomography with Lugol\u2019s Solution Enhancement"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5572-1364","authenticated-orcid":false,"given":"Kyu-Ho","family":"Yi","sequence":"first","affiliation":[{"name":"Division in Anatomy and Developmental Biology, Department of Oral Biology, Human Identification Research Institute, BK21 FOURProject, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seoul 03722, Republic of Korea"},{"name":"Maylin Clinic (Apgujeong), Seoul 07335, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA 02215, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3732-2698","authenticated-orcid":false,"given":"Ji-Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Acupoint, College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3082-935X","authenticated-orcid":false,"given":"Hyung-Jin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Catholic Institute for Applied Anatomy, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,20]]},"reference":[{"key":"ref_1","first-page":"bcr2016218280","article-title":"Non-recurrent laryngeal nerve with a coexisting contralateral nerve demonstrating extralaryngeal branching","volume":"2017","author":"Constable","year":"2017","journal-title":"Case Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1590\/S0102-865020160070000003","article-title":"Surgical approach to the thyroarytenoid branch of the inferior laryngeal nerve through the thyroid cartilage","volume":"31","author":"Sperandio","year":"2016","journal-title":"Acta Cir\u00fargica Bras."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Severin, F., Rosu, A.-M., Tiglis, M., Checherita, L.-E., Stegaru, G., Cobzeanu, M.D., Hainarosie, R., Cobzeanu, B.M., and Palade, O.D. 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