{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:12:18Z","timestamp":1790874738651,"version":"4.1.0"},"reference-count":49,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T00:00:00Z","timestamp":1695859200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry for Education, University and Research (Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca\u2014MIUR)","award":["\u2018Dipartimenti di Eccellenza 2018\u20132022: Progetto DIMAR 2018\u20132022\u2032"],"award-info":[{"award-number":["\u2018Dipartimenti di Eccellenza 2018\u20132022: Progetto DIMAR 2018\u20132022\u2032"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background: The usefulness of myositis-specific autoantibodies (MSAs) and myositis-associated autoantibodies (MAAs) for the assessment of idiopathic inflammatory myopathies (IIMs) is acknowledged, but laboratory standardization remains a challenge. We detected MSAs\/MAAs by multi-analytic line immunoassay (LIA) and particle-based multi-analyte technology (PMAT) in a multicenter cohort of patients with IIMs. Methods: We tested the sera from 411 patients affected with definite IIM, including 142 polymyositis (PM), 147 dermatomyositis (DM), 19 cancer-associated myositis, and 103 overlap myositis syndrome (OM), and from 269 controls. MSAs\/MAAs were determined by 16Ags LIA in all sera, and anti-HMGCR by ELISA in 157\/411 IIM sera and 91\/269 control sera. The analytical specificity of LIA\/HMGCR ELISA was compared with that of PMAT in 89 MSA+ IIM sera. Results: MSAs\/MAAs were positive in 307\/411 (75%) IIM patients and 65\/269 (24%) controls by LIA (Odds Ratio 9.26, 95% CI 6.43\u201313.13, p &lt; 0.0001). The sensitivity\/specificity of individual MSAs\/MAAs were: 20%\/100% (Jo-1), 3%\/99.3% (PL-7), 4%\/98.8% (PL-12), 1%\/100% (EJ), 0.7%\/100% (OJ), 9%\/98% (SRP), 5.6%\/99.6% (TIF1\u03b3), 4.6%\/99.6% (MDA5), 8%\/96% (Mi-2), 1.5%\/98% (NXP2), 1.7%\/100% (SAE1), 4%\/92% (Ku), 8.5%\/99% (PM\/Scl-100), 8%\/96% (PM\/Scl-75), and 25.5%\/79% (Ro52). Anti-HMGCR was found in 8\/157 (5%) IIM patients and 0\/176 (0%) controls by ELISA (p = 0.007). Concordance between LIA\/HMGCR ELISA and PMAT was found in 78\/89 (88%) samples. Individual MSAs detected by LIA were associated with IIM subsets: Jo-1 with PM and OM, PL-12 with OM, Mi-2, TIF1\u03b3, and MDA5 with DM, SRP with PM, and PM\/Scl-75\/100 with OM (p &lt; 0.001 for all). Conclusions: Since MSAs are mostly mutually exclusive, multi-specific antibody profiling seems effective for a targeted clinical-serologic approach to the diagnosis of IIMs.<\/jats:p>","DOI":"10.3390\/diagnostics13193080","type":"journal-article","created":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T02:47:30Z","timestamp":1695955650000},"page":"3080","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Detection of Myositis Autoantibodies by Multi-Analytic Immunoassays in a Large Multicenter Cohort of Patients with Definite Idiopathic Inflammatory Myopathies"],"prefix":"10.3390","volume":"13","author":[{"given":"Anna","family":"Ghirardello","sequence":"first","affiliation":[{"name":"Rheumatology Unit, Department of Medicine-DIMED, University Hospital of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4012-1248","authenticated-orcid":false,"given":"Mariele","family":"Gatto","sequence":"additional","affiliation":[{"name":"Rheumatology Unit, Department of Medicine-DIMED, University Hospital of Padova, 35128 Padova, Italy"},{"name":"Rheumatology Unit, Department of Clinical and Biological Sciences, Mauriziano Hospital, University of Turin, 10124 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9027-511X","authenticated-orcid":false,"given":"Chiara","family":"Franco","sequence":"additional","affiliation":[{"name":"Rheumatology Unit, Department of Medicine-DIMED, University Hospital of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Zanatta","sequence":"additional","affiliation":[{"name":"Rheumatology Unit, Department of Medicine-DIMED, University Hospital of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2356-375X","authenticated-orcid":false,"given":"Roberto","family":"Padoan","sequence":"additional","affiliation":[{"name":"Rheumatology Unit, Department of Medicine-DIMED, University Hospital of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana","family":"Ienna","sequence":"additional","affiliation":[{"name":"Rheumatology Unit, Department of Medicine-DIMED, University Hospital of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicoletta","family":"Gallo","sequence":"additional","affiliation":[{"name":"Unit of Laboratory Medicine, Department of Medicine-DIMED, University Hospital of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0835-1406","authenticated-orcid":false,"given":"Margherita","family":"Zen","sequence":"additional","affiliation":[{"name":"Rheumatology Unit, Department of Medicine-DIMED, University Hospital of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6068-9212","authenticated-orcid":false,"given":"Ingrid E.","family":"Lundberg","sequence":"additional","affiliation":[{"name":"Rheumatology Unit, Department of Medicine, Karolinska University Hospital in Solna, Karolinska Institutet, 171 77 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Mahler","sequence":"additional","affiliation":[{"name":"Werfen Autoimmunity, San Diego, CA 92131, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0548-4983","authenticated-orcid":false,"given":"Andrea","family":"Doria","sequence":"additional","affiliation":[{"name":"Rheumatology Unit, Department of Medicine-DIMED, University Hospital of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Iaccarino","sequence":"additional","affiliation":[{"name":"Rheumatology Unit, Department of Medicine-DIMED, University Hospital of Padova, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Yildiz, H., Lepere, C., Zorzi, G., Gheysens, O., Roodhans, F., and Pothen, L. 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