{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T11:32:54Z","timestamp":1789471974058,"version":"build-2803163510"},"reference-count":24,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:00:00Z","timestamp":1696982400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background: Childhood chronic non-infectious uveitis (cNIU) is a challenging disease that needs close monitoring. Slit lamp evaluation (SLE) is the cornerstone of ophthalmological evaluation for uveitis, but it is affected by interobserver variability and may be problematic in children. Laser flare photometry (LFP), a novel and objective technique, might be used in children with uveitis. Aim: The aim of this study was to attempt the use of LFP in cNIU clinical practice. Methods: Children, attending the Rheumatology Unit and who were scheduled to receive ophthalmological evaluation, were prospectively enrolled to concomitantly receive SLE and LFP. SLE was performed blind to LFP measure. Demographic, laboratory, clinical, and ophthalmology data were collected. Results: A total of 29 children (58 eyes) were enrolled, including 3 with juvenile idiopathic arthritis without uveitis (JIA-no-U), 15 with JIA-associated uveitis (JIA-U), and 11 with idiopathic chronic uveitis (ICU). We observed significantly higher LFP values in the eyes of children with uveitis compared to the others (10.1 IQR 7.1\u201313.6 versus 6.2 IQR 5.8\u20136.9, p = 0.007). Accordance between the SLE and LFP measures, at baseline (\u03c1.498, p &lt; 0.001) and during the follow-up (LFP II \u03c1 0.460, p &lt; 0.001, LFP III \u03c1 0.631, p &lt; 0.001, LFP IV \u03c1 0.547, p = 0.006, LFP V \u03c1 0.767, p = 0.001), was detected. We evaluated significant correlation between LFP values and the presence of complications (\u03c1 0.538, p &lt; 0.001), especially with cataract formation (\u03c1 0.542, p &lt; 0.001). Conclusions: In this cohort, LFP measurements showed a good correlation with SLE. LFP values showed a positive correlation with the presence of complications. LFP might be considered as a reliable objective modality to monitor intraocular inflammation in cNIU.<\/jats:p>","DOI":"10.3390\/diagnostics13203179","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T08:07:12Z","timestamp":1697011632000},"page":"3179","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Laser Flare Photometry to Monitor Childhood Chronic Uveitis: A Preliminary Report of a Monocentric Italian Experience"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6418-3254","authenticated-orcid":false,"given":"Ilaria","family":"Maccora","sequence":"first","affiliation":[{"name":"Rheumatology Unit, ERN ReConnet Center, Meyer Children\u2019s Hospital IRCCS, 50139 Florence, Italy"},{"name":"NeuroFARBA Department, University of Florence, 50121 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cinzia","family":"De Libero","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, Meyer Children\u2019s Hospital IRCCS, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matilde","family":"Peri","sequence":"additional","affiliation":[{"name":"School of Health Human Science, University of Florence, 50121 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gioia","family":"Danti","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, Meyer Children\u2019s Hospital IRCCS, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessio","family":"Rossi","sequence":"additional","affiliation":[{"name":"School of Health Human Science, University of Florence, 50121 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0346-3268","authenticated-orcid":false,"given":"Edoardo","family":"Marrani","sequence":"additional","affiliation":[{"name":"Rheumatology Unit, ERN ReConnet Center, Meyer Children\u2019s Hospital IRCCS, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Pasqualetti","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, Meyer Children\u2019s Hospital IRCCS, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Pagnini","sequence":"additional","affiliation":[{"name":"Rheumatology Unit, ERN ReConnet Center, Meyer Children\u2019s Hospital IRCCS, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9784-3543","authenticated-orcid":false,"given":"Maria Vincenza","family":"Mastrolia","sequence":"additional","affiliation":[{"name":"Rheumatology Unit, ERN ReConnet Center, Meyer Children\u2019s Hospital IRCCS, 50139 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5919-4861","authenticated-orcid":false,"given":"Gabriele","family":"Simonini","sequence":"additional","affiliation":[{"name":"Rheumatology Unit, ERN ReConnet Center, Meyer Children\u2019s Hospital IRCCS, 50139 Florence, Italy"},{"name":"NeuroFARBA Department, University of Florence, 50121 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/09273948.2023.2169715","article-title":"Childhood Chronic Idiopathic Uveitis in a Multicentre International Cohort","volume":"21","author":"Maccora","year":"2023","journal-title":"Ocul. Immunol. Inflamm."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1080\/1744666X.2023.2198210","article-title":"Challenges and management of childhood noninfectious chronic uveitis","volume":"19","author":"Maccora","year":"2023","journal-title":"Expert. Rev. Clin. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1097\/BOR.0000000000000723","article-title":"Update on noninfectious uveitis in children and its treatment","volume":"32","author":"Maccora","year":"2020","journal-title":"Curr. Opin. Rheumatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/S0002-9394(02)02148-7","article-title":"Visual loss associated with pediatric uveitis in English primary and referral centers","volume":"135","author":"Edelsten","year":"2003","journal-title":"Am. J. Ophthalmol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.ajo.2005.03.057","article-title":"Standardization of uveitis nomenclature for reporting clinical data. Results of the First International Workshop","volume":"140","author":"Jabs","year":"2005","journal-title":"Am. J. Ophthalmol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/s12969-018-0266-5","article-title":"Outcomes of non-infectious Paediatric uveitis in the era of biologic therapy","volume":"16","author":"Cann","year":"2018","journal-title":"Pediatr. Rheumatol. Online J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1002\/acr.23870","article-title":"2019 American College of Rheumatology\/Arthritis Foundation Guideline for the Screening, Monitoring, and Treatment of Juvenile Idiopathic Arthritis-Associated Uveitis","volume":"71","author":"Ringold","year":"2019","journal-title":"Arthritis Care Res."},{"key":"ref_8","first-page":"344","article-title":"A reconsideration of anterior chamber flare and its clinical relevance for children with chronic anterior uveitis (an American Ophthalmological Society thesis)","volume":"105","author":"Holland","year":"2007","journal-title":"Trans. Am. Ophthalmol. Soc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1001\/archopht.119.12.1763","article-title":"Relationships between laser flare photometry values and complications of uveitis","volume":"119","author":"Gonzales","year":"2001","journal-title":"Arch. Ophthalmol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.survophthal.2004.10.004","article-title":"Laser flare-cell photometry: Methodology and clinical applications","volume":"50","author":"Ladas","year":"2005","journal-title":"Surv. Ophthalmol."},{"key":"ref_11","first-page":"390","article-title":"International League of Associations for Rheumatology classification of juvenile idiopathic arthritis: Second revision, Edmonton, 2001","volume":"31","author":"Petty","year":"2004","journal-title":"J. Rheumatol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1167\/iovs.05-0981","article-title":"Visual acuities \u201chand motion\u201d and \u201ccounting fingers\u201d can be quantified with the freiburg visual acuity test","volume":"47","author":"Feltgen","year":"2006","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1177\/11206721221107303","article-title":"Comparative study of laser flare photometry versus slit-lamp cell measurement in pediatric chronic non-infectious anterior uveitis","volume":"33","author":"Temel","year":"2023","journal-title":"Eur. J. Ophthalmol."},{"key":"ref_14","first-page":"662","article-title":"The Value of Laser Flare Photometry as a Monitoring Tool for Uveitis","volume":"240","author":"Tappeiner","year":"2023","journal-title":"Klin. Monatsblatter Augenheilkd."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/S0002-9394(03)00315-5","article-title":"Laser flare photometry and complications of chronic uveitis in children","volume":"135","author":"Davis","year":"2003","journal-title":"Am. J. Ophthalmol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1080\/09273948.2019.1640883","article-title":"Instrument-based Tests for Measuring Anterior Chamber Cells in Uveitis: A Systematic Review","volume":"28","author":"Liu","year":"2020","journal-title":"Ocul. Immunol. Inflamm."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"334","DOI":"10.3109\/09273948.2010.512994","article-title":"Controversies in ocular inflammation and immunology laser flare photometry","volume":"18","author":"Wakefield","year":"2010","journal-title":"Ocul. Immunol. Inflamm."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s10384-016-0488-3","article-title":"Laser flare-cell photometer: Principle and significance in clinical and basic ophthalmology","volume":"61","author":"Sawa","year":"2017","journal-title":"Jpn. J. Ophthalmol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.ajo.2022.05.012","article-title":"Quantification of Anterior Chamber Cells in Children With Uveitis Using Anterior Segment Optical Coherence Tomography","volume":"241","author":"Tsui","year":"2022","journal-title":"Am. J. Ophthalmol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/S0161-6420(97)30359-5","article-title":"Use of laser flare photometry to assess and monitor inflammation in uveitis","volume":"104","author":"Herbort","year":"1997","journal-title":"Ophthalmology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1080\/09273948.2020.1792511","article-title":"Laser Flare Photometry: A Useful Tool for Monitoring Patients with Juvenile Idiopathic Arthritis-associated Uveitis","volume":"30","author":"Terrada","year":"2022","journal-title":"Ocul. Immunol. Inflamm."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1136\/annrheumdis-2017-212089","article-title":"ADJUVITE: A double-blind, randomised, placebo-controlled trial of adalimumab in early onset, chronic, juvenile idiopathic arthritis-associated anterior uveitis","volume":"77","author":"Quartier","year":"2018","journal-title":"Ann. Rheum. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.1007\/s10792-021-01779-0","article-title":"Laser flare and cell photometry to measure inflammation after cataract surgery: A tool to predict the risk of cystoid macular edema","volume":"41","author":"Coassin","year":"2021","journal-title":"Int. Ophthalmol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3223","DOI":"10.1007\/s12325-020-01383-1","article-title":"Persistence of Inflammation After Uncomplicated Cataract Surgery: A 6-Month Laser Flare Photometry Analysis","volume":"37","author":"Coassin","year":"2020","journal-title":"Adv. Ther."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/13\/20\/3179\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:05:02Z","timestamp":1760130302000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/13\/20\/3179"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,11]]},"references-count":24,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["diagnostics13203179"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics13203179","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,11]]}}}