{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:40:30Z","timestamp":1760143230942,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T00:00:00Z","timestamp":1706486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Health &amp; Welfare, Republic of Korea","award":["HI19C1046","20210048001"],"award-info":[{"award-number":["HI19C1046","20210048001"]}]},{"name":"Korea Health Industry Development Institute","award":["HI19C1046","20210048001"],"award-info":[{"award-number":["HI19C1046","20210048001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>(1) Background: An early mesothelial reaction of the pleura, leading to fibrosis, has been reported in animals after chemical or heavy metal exposure. However, the visual monitoring of early time-sequential mesothelial reaction-associated cryoinjury has not been fully investigated. Therefore, this study aimed to evaluate and visualize the early mesothelial reactions seen following cryoinjury using rabbit pleura. (2) Methods: We monitored the early mesothelial reaction in rabbit pleurae after cryoinjury using optical coherence tomography (OCT), in real-time, which was then compared with pathological images. Due to the penetration limit of OCT, we made a thoracic window to image the parietal and visceral pleurae in vivo. We also used an innovative technique for capturing the microstructure in vivo, employing a computer-controlled intermittent iso-pressure breath hold to reduce respiratory motion, increasing the resolution of OCT. We organized three sample groups: the normal group, the sham group with just a thoracic window, and the experimental group with a thoracic window and cryotherapy. In the experimental group, localized cryoinjury was performed. The mesothelial cells at the level of pleura of the cryotherapy-injured site were visualized by OCT within the first 30 min and then again after 2 days at the same site. (3) Results: In the experimental group, focal thickening of the parietal pleura was observed at the site of cryoinjury using OCT after the first injury, and it was then confirmed pathologically as focal mesothelial cell proliferation. Two days after cryoinjury, diffuse mesothelial cell proliferation in the parietal pleura was noted on the reverse side around the cryoinjured site in the same rabbit. In the sham group, no pleural reaction was found. The OCT and pathological examinations revealed different patterns of mesothelial cell reactions between the parietal and visceral pleurae: the focal proliferation of mesothelial cells was found in the parietal pleura, while only a morphological change from flat cells to cuboidal cells and a thickened monolayer without proliferation of mesothelial cells were found in the visceral pleural. (4) Conclusions: An early mesothelial reaction occurs following cryoinjury to the parietal and visceral pleurae.<\/jats:p>","DOI":"10.3390\/diagnostics14030292","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T08:54:03Z","timestamp":1706518443000},"page":"292","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Time-Sequential Monitoring of the Early Mesothelial Reaction in the Pleura after Cryoinjury"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7786-5051","authenticated-orcid":false,"given":"Taeyun","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Samsung Medical Center, Seoul 06351, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Kyung","family":"Chae","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung-Jin","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Kosin University College of Medicine, Busan 49267, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haeyoung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery, Kosin University College of Medicine, Busan 46241, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang-Suk","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Kosin University College of Medicine, Busan 49267, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun-Kee","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Medical Humanities and Social Medicine, Kosin University College of Medicine, Busan 46241, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeh-Chan","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9187-4675","authenticated-orcid":false,"given":"Chulho","family":"Oak","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Kosin University College of Medicine, Busan 49267, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1159\/000113629","article-title":"Pathophysiology of the pleura","volume":"75","author":"Jantz","year":"2008","journal-title":"Respiration"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1002\/ijc.25619","article-title":"Tumor associated mesenchymal stem cells protects ovarian cancer cells from hyperthermia through CXCL12","volume":"128","author":"Lis","year":"2011","journal-title":"Int. J. Cancer"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"81","DOI":"10.3109\/10408448209041321","article-title":"The mesothelium and its reactions: A review","volume":"10","author":"Whitaker","year":"1982","journal-title":"Crit. Rev. Toxicol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1165\/ajrcmb.10.3.7509611","article-title":"Mesothelial cell proliferation: A nonspecific response to lung injury associated with fibrosis","volume":"10","author":"Adamson","year":"1994","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1183\/09031936.06.00037906","article-title":"Evidence that mesothelial cells regulate the acute inflammatory response in talc pleurodesis","volume":"28","author":"Marchi","year":"2006","journal-title":"Eur. Respir. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1172\/JCI115710","article-title":"Evidence of a role for mesothelial cell-derived interleukin 8 in the pathogenesis of asbestos-induced pleurisy in rabbits","volume":"89","author":"Boylan","year":"1992","journal-title":"J. Clin. Investig."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1006\/faat.1997.2344","article-title":"Mesothelial cell proliferation and biopersistence of wollastonite and crocidolite asbestos fibers","volume":"38","author":"Macdonald","year":"1997","journal-title":"Fundam. Appl. Toxicol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1038\/nm0898-929","article-title":"Regenerating functional myocardium: Improved performance after skeletal myoblast transplantation","volume":"4","author":"Taylor","year":"1998","journal-title":"Nat. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"H1291","DOI":"10.1152\/ajpheart.00111.2005","article-title":"A novel model of cryoinjury-induced myocardial infarction in the mouse: A comparison with coronary artery ligation","volume":"289","author":"Mees","year":"2005","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/0011-2240(82)90151-1","article-title":"Immunological aspects of cryosurgery in general surgery","volume":"19","author":"Tanaka","year":"1982","journal-title":"Cryobiology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/S0039-6060(99)70093-0","article-title":"Acute lung injury after hepatic cryoablation: Correlation with NF-kappa B activation and cytokine production","volume":"126","author":"Blackwell","year":"1999","journal-title":"Surgery"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/jsre.2000.5976","article-title":"Hepatic cryoablation-induced acute lung injury: Histopathologic findings","volume":"95","author":"Washington","year":"2001","journal-title":"J. Surg. Res."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Brenner, M., Kreuter, K., Ju, J., Mahon, S., Tseng, L., Mukai, D., Burney, T., Guo, S., Su, J., and Tran, A. (2008). In vivo optical coherence tomography detection of differences in regional large airway smoke inhalation induced injury in a rabbit model. J. Biomed. Opt., 13.","DOI":"10.1117\/1.2939400"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1364\/BOE.2.000243","article-title":"Quantification of airway thickness changes in smoke-inhalation injury using in-vivo 3-D endoscopic frequency-domain optical coherence tomography","volume":"2","author":"Lee","year":"2011","journal-title":"Biomed. Opt. Express"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Kreuter, K.A., Mahon, S.B., Mukai, D.S., Su, J., Jung, W.G., Narula, N., Guo, S., Wakida, N., Raub, C., and Berns, M.W. (2009). Detection and monitoring of early airway injury effects of half-mustard (2-chloroethylethylsulfide) exposure using high-resolution optical coherence tomography. J. Biomed. Opt., 14.","DOI":"10.1117\/1.3210775"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Meissner, S., Knels, L., Schnabel, C., Koch, T., and Koch, E. (2010). Three-dimensional Fourier domain optical coherence tomography in vivo imaging of alveolar tissue in the intact thorax using the parietal pleura as a window. J. Biomed. Opt., 15.","DOI":"10.1117\/1.3302809"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/s00408-007-9041-y","article-title":"Pleural mesothelial cells mediate inflammatory and profibrotic responses in talc-induced pleurodesis","volume":"185","author":"Acencio","year":"2007","journal-title":"Lung"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1440-1843.2005.00694.x","article-title":"Pathogenesis of malignant pleural mesothelioma","volume":"10","author":"Jaurand","year":"2005","journal-title":"Respirology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2411","DOI":"10.1183\/09031936.97.10102411","article-title":"Immunobiology of pleural inflammation: Potential implications for pathogenesis, diagnosis and therapy","volume":"10","author":"Kroegel","year":"1997","journal-title":"Eur. Respir. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1136\/jclinpath-2016-203945","article-title":"Malignant peritoneal mesothelioma and Crohn disease","volume":"70","author":"Butnor","year":"2017","journal-title":"J. Clin. Pathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/S0002-9440(10)64888-2","article-title":"Stimulation of mesothelial cell proliferation by exudate macrophages enhances serosal wound healing in a murine model","volume":"160","author":"Mutsaers","year":"2002","journal-title":"Am. J. Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"607","DOI":"10.3807\/JOSK.2016.20.5.607","article-title":"Ex vivo high-resolution optical coherence tomography (OCT) imaging of pleural reaction after pleurodesis using talc","volume":"20","author":"Ahn","year":"2016","journal-title":"J. Opt. Soc. Korea"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s00595-010-4275-3","article-title":"Changes in the pleural cavity by pleurodesis using talc or OK-432: An experimental study","volume":"41","author":"Muta","year":"2011","journal-title":"Surg. Today"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1136\/thx.32.4.377","article-title":"Relationship between occupations and asbestos-fibre content of the lungs in patients with pleural mesothelioma, lung cancer, and other diseases","volume":"32","author":"Whitwell","year":"1977","journal-title":"Thorax"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.lungcan.2009.03.016","article-title":"Malignant pleural mesothelioma: Genome-wide expression patterns reflecting general resistance mechanisms and a proposal of novel targets","volume":"67","author":"Anderssen","year":"2010","journal-title":"Lung Cancer"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"R\u00f8e, O.D., Anderssen, E., Helge, E., Pettersen, C.H., Olsen, K.S., Sandeck, H., Haaverstad, R., Lundgren, S., and Larsson, E. (2009). Genome-wide profile of pleural mesothelioma versus parietal and visceral pleura: The emerging gene portrait of the mesothelioma phenotype. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0006554"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Xie, T., Liu, G., Kreuter, K., Mahon, S., Colt, H., Mukai, D., Peavy, G.M., Chen, Z., and Brenner, M. (2009). In vivo three-dimensional imaging of normal tissue and tumors in the rabbit pleural cavity using endoscopic swept source optical coherence tomography with thoracoscopic guidance. J. Biomed. Opt., 14.","DOI":"10.1117\/1.3275478"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1586\/ers.11.59","article-title":"Recent advances in optical coherence tomography for the diagnoses of lung disorders","volume":"5","author":"Hou","year":"2011","journal-title":"Expert Rev. Respir. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.jtos.2015.02.001","article-title":"High-Resolution Optical Coherence Tomography as an Adjunctive Tool in the Diagnosis of Corneal and Conjunctival Pathology","volume":"13","author":"Nanji","year":"2015","journal-title":"Ocul. Surf."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/j.ophtha.2012.10.025","article-title":"Diagnosis of ocular surface lesions using ultra-high-resolution optical coherence tomography","volume":"120","author":"Shousha","year":"2013","journal-title":"Ophthalmology"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Bai, K.J., Chuang, K.J., Chen, J.K., Tsai, C.Y., Yang, Y.L., Chang, C.C., Chen, T.T., Lee, C.N., Feng, P.H., and Chen, K.Y. (2019). Alterations by Air Pollution in Inflammation and Metals in Pleural Effusion of Pneumonia Patients. Int. J. Environ. Res. Public. Health, 16.","DOI":"10.3390\/ijerph16050705"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.envres.2019.03.006","article-title":"Pleural anthracosis as an indicator of lifetime exposure to urban air pollution: An autopsy-based study in Sao Paulo","volume":"173","author":"Takano","year":"2019","journal-title":"Environ. Res."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/14\/3\/292\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:51:16Z","timestamp":1760104276000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/14\/3\/292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,29]]},"references-count":32,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["diagnostics14030292"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics14030292","relation":{},"ISSN":["2075-4418"],"issn-type":[{"type":"electronic","value":"2075-4418"}],"subject":[],"published":{"date-parts":[[2024,1,29]]}}}