{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:21:15Z","timestamp":1780446075753,"version":"3.54.1"},"reference-count":41,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T00:00:00Z","timestamp":1708473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education and Science of the Russian Federation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background: NK cells phenotype and functional state in different genotypes of chronic viral hepatitis C (CVHC), depending on liver fibrosis severity, have not been sufficiently studied, which limits the possibilities for the development of pathology therapy. Methods: The CVHC diagnosis was based on the EASL recommendations (2018). Clinical examination with liver elastometry was performed in 297 patients with genotype 1 and in 231 patients with genotype 3 CVHC. The blood NK cells phenotype was determined by flow cytometry in 74 individuals with genotype 1 and in 69 individuals with genotype 3 CVHC. Results: The frequency of METAVIR liver fibrosis stages F3\u2013F4 was 32.5% in individuals with genotype 3, and 20.5% in individuals with genotype 1 CVHC (p = 0.003). In patients with both genotype 1 and genotype 3 CVHC, a decrease in the total number of blood NK cells, CD56brightCD16+ NK cells and an increase in the proportion of CD56dimCD16+ NK cells, CD94+ and CD38 + CD73+ NK cells were registered in patients with fibrosis stage F3\u2013F4 by METAVIR in comparison with persons with METAVIR fibrosis stage F0\u2013F1. Conclusions: In patients with both genotype 1 and genotype 3 CVHC, an imbalance in the ratio between cytokine-producing and cytotoxic NK cells and an increase in the content of NK cells that express inhibitory molecules were determined in patients with severe liver fibrosis.<\/jats:p>","DOI":"10.3390\/diagnostics14050472","type":"journal-article","created":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T10:52:00Z","timestamp":1708512720000},"page":"472","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Association of Blood NK Cell Phenotype with the Severity of Liver Fibrosis in Patients with Chronic Viral Hepatitis C with Genotype 1 or 3"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9980-2294","authenticated-orcid":false,"given":"Vladislav Vladimirovich","family":"Tsukanov","sequence":"first","affiliation":[{"name":"Federal Research Center \u201cKrasnoyarsk Science Center\u201d of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North, 660022 Krasnoyarsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5829-672X","authenticated-orcid":false,"given":"Andrei Anatolyevich","family":"Savchenko","sequence":"additional","affiliation":[{"name":"Federal Research Center \u201cKrasnoyarsk Science Center\u201d of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North, 660022 Krasnoyarsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail Aleksandrovich","family":"Cherepnin","sequence":"additional","affiliation":[{"name":"Federal Research Center \u201cKrasnoyarsk Science Center\u201d of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North, 660022 Krasnoyarsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduard Vilyamovich","family":"Kasparov","sequence":"additional","affiliation":[{"name":"Federal Research Center \u201cKrasnoyarsk Science Center\u201d of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North, 660022 Krasnoyarsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena Petrovna","family":"Tikhonova","sequence":"additional","affiliation":[{"name":"Krasnoyarsk State Medical University Named after Prof. V.F. Voino-Yasenetsky of the Ministry of Healthcare of Russian Federation, 660022 Krasnoyarsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander Viktorovich","family":"Vasyutin","sequence":"additional","affiliation":[{"name":"Federal Research Center \u201cKrasnoyarsk Science Center\u201d of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North, 660022 Krasnoyarsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Leongardovna","family":"Tonkikh","sequence":"additional","affiliation":[{"name":"Federal Research Center \u201cKrasnoyarsk Science Center\u201d of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North, 660022 Krasnoyarsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Alexandrovna","family":"Anisimova","sequence":"additional","affiliation":[{"name":"Krasnoyarsk State Medical University Named after Prof. V.F. Voino-Yasenetsky of the Ministry of Healthcare of Russian Federation, 660022 Krasnoyarsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2848-0846","authenticated-orcid":false,"given":"Vasily Dmitrievich","family":"Belenyuk","sequence":"additional","affiliation":[{"name":"Federal Research Center \u201cKrasnoyarsk Science Center\u201d of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North, 660022 Krasnoyarsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9026-2615","authenticated-orcid":false,"given":"Alexandr Gennadyevich","family":"Borisov","sequence":"additional","affiliation":[{"name":"Federal Research Center \u201cKrasnoyarsk Science Center\u201d of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North, 660022 Krasnoyarsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.virusres.2018.02.016","article-title":"Critical challenges and emerging opportunities in hepatitis C virus research in an era of potent antiviral therapy: Considerations for scientists and funding agencies","volume":"248","author":"Bartenschlager","year":"2018","journal-title":"Virus Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"33","DOI":"10.4103\/0974-777X.52979","article-title":"Association between hepatitis C and hepatocellular carcinoma","volume":"1","author":"Melo","year":"2009","journal-title":"J. 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