{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T01:11:23Z","timestamp":1788311483691,"version":"build-2803163510"},"reference-count":21,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T00:00:00Z","timestamp":1712793600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Generalitat de Catalunya","award":["PI20\/00766"],"award-info":[{"award-number":["PI20\/00766"]}]},{"name":"Generalitat de Catalunya","award":["2021SGR01569"],"award-info":[{"award-number":["2021SGR01569"]}]},{"name":"Instituto de Salud Carlos III [Carlos III Institute of Health]","award":["PI20\/00766"],"award-info":[{"award-number":["PI20\/00766"]}]},{"name":"Instituto de Salud Carlos III [Carlos III Institute of Health]","award":["2021SGR01569"],"award-info":[{"award-number":["2021SGR01569"]}]},{"name":"Ag\u00e8ncia de Gesti\u00f3 d\u2019Ajuts Universitaris i de Recerca of the Generalitat de Catalunya [Agency for Management of University and Research Grants of the Catalan Government]","award":["PI20\/00766"],"award-info":[{"award-number":["PI20\/00766"]}]},{"name":"Ag\u00e8ncia de Gesti\u00f3 d\u2019Ajuts Universitaris i de Recerca of the Generalitat de Catalunya [Agency for Management of University and Research Grants of the Catalan Government]","award":["2021SGR01569"],"award-info":[{"award-number":["2021SGR01569"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>The rapid and broad microbiological diagnosis of meningoencephalitis (ME) has been possible thanks to the development of multiplex PCR tests applied to cerebrospinal fluid (CSF). We aimed to assess a new multiplex PCR panel (the QIAstat-Dx ME panel), which we compared to conventional diagnostic tools and the Biofire FilmArray ME Panel. The pathogens analyzed using both methods were Escherichia coli K1, Haemophilus influenzae, Listeria monocytogenes, Neisseria meningitidis, Streptococcus agalactiae, Streptococcus pneumoniae, Enterovirus, herpes simplex virus 1\u20132, human herpesvirus 6, human parechovirus, varicella zoster virus, and Cryptococcus neoformans\/gattii. We used sensitivity, specificity, PPV, NPV, and kappa correlation index parameters to achieve our objective. Fifty CSF samples from patients with suspected ME were included. When conventional methods were used, 28 CSF samples (56%) were positive. The sensitivity and specificity for QIAstat-Dx\/ME were 96.43% (CI95%, 79.8\u201399.8) and 95.24% (75.2\u201399.7), respectively, whereas the PPV and NPV were 96.43% (79.8\u201399.8) and 95.24% (75.1\u201399.7), respectively. The kappa value was 91.67%. Conclusions: A high correlation of the QIAstat-Dx ME panel with reference methods was shown. QIAstat-Dx ME is a rapid-PCR technique to be applied in patients with suspected ME with a high accuracy.<\/jats:p>","DOI":"10.3390\/diagnostics14080802","type":"journal-article","created":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T07:09:28Z","timestamp":1712819368000},"page":"802","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["An Assessment of a New Rapid Multiplex PCR Assay for the Diagnosis of Meningoencephalitis"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9031-0787","authenticated-orcid":false,"given":"Genoveva","family":"Cuesta","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Hospital Clinic, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Puerta-Alcalde","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Hospital Cl\u00ednic-IDIBAPS, University of Barcelona, 08007 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5046-4490","authenticated-orcid":false,"given":"Andrea","family":"Vergara","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Hospital Clinic, 08036 Barcelona, Spain"},{"name":"Department of Basic Clinical Practice, Faculty of Medicine, University of Barcelona, 08007 Barcelona, Spain"},{"name":"CIBER Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Institute of Global Health of Barcelona (ISGlobal), 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enric","family":"Roses","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Hospital Clinic, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Bosch","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Hospital Clinic, 08036 Barcelona, Spain"},{"name":"Institute of Global Health of Barcelona (ISGlobal), 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Climent","family":"Casals-Pascual","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Hospital Clinic, 08036 Barcelona, Spain"},{"name":"Department of Basic Clinical Practice, Faculty of Medicine, University of Barcelona, 08007 Barcelona, Spain"},{"name":"CIBER Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Institute of Global Health of Barcelona (ISGlobal), 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9374-0811","authenticated-orcid":false,"given":"Alex","family":"Soriano","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Hospital Cl\u00ednic-IDIBAPS, University of Barcelona, 08007 Barcelona, Spain"},{"name":"CIBER Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00aa \u00c1ngeles","family":"Marcos","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Hospital Clinic, 08036 Barcelona, Spain"},{"name":"Department of Basic Clinical Practice, Faculty of Medicine, University of Barcelona, 08007 Barcelona, Spain"},{"name":"CIBER Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Institute of Global Health of Barcelona (ISGlobal), 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5010-4527","authenticated-orcid":false,"given":"Sergi","family":"Sanz","sequence":"additional","affiliation":[{"name":"Department of Basic Clinical Practice, Faculty of Medicine, University of Barcelona, 08007 Barcelona, Spain"},{"name":"Institute of Global Health of Barcelona (ISGlobal), 08036 Barcelona, Spain"},{"name":"CIBER Epidemiolog\u00eda y Salud P\u00fablica (CIBERESP), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8025-3926","authenticated-orcid":false,"given":"Jordi","family":"Vila","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Hospital Clinic, 08036 Barcelona, Spain"},{"name":"Department of Basic Clinical Practice, Faculty of Medicine, University of Barcelona, 08007 Barcelona, Spain"},{"name":"CIBER Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Institute of Global Health of Barcelona (ISGlobal), 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s15010-007-6202-0","article-title":"Community-acquired bacterial meningitis in adults: Antibiotic timing in disease course and outcome","volume":"35","author":"Lepur","year":"2007","journal-title":"Infection"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1354","DOI":"10.1093\/cid\/civ574","article-title":"Central Nervous System Infections in Childhood","volume":"68","author":"Wormser","year":"2015","journal-title":"Clin. 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