{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T02:54:25Z","timestamp":1771383265704,"version":"3.50.1"},"reference-count":27,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T00:00:00Z","timestamp":1713398400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Nam\u0131k Kemal University Scientific Research Projects Coordination Unit","award":["NKUBAP.00.20.AR.13.01"],"award-info":[{"award-number":["NKUBAP.00.20.AR.13.01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Recent studies have focused on the role of human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7) in PR etiology with varying results. In our study, with the approach that the discrepancy between the results may be related to the different samples and techniques used, we aimed to clarify the etiology by examining tissue and plasma samples using molecular methods and evaluating the results together with serological parameters. Skin biopsies and plasma samples of twenty-five PR patients were tested to detect HHV-6 and HHV-7 DNA using calibrated quantitative real-time polymerase chain reaction (CQ RT-PCR). IgG and IgM antibodies against HHV-6 and HHV-7 were tested by enzyme-linked immunosorbent assay and indirect immunofluorescence. Of the patient group, 64% were positive for HHV-6 IgG without IgM positivity. HHV-6 DNA was present in seven tissue and ten plasma samples. HHV-7 positivity was 100% and 12% for IgG and IgM antibodies, respectively. HHV-7 DNA was detected in four tissue samples and one plasma sample. Patients with HHV-7 DNA-positive plasma and tissue samples had also HHV-7 IgM antibodies. In conclusion, our results seem to support the role of HHV-6\/HHV-7 in the etiology of PR. To clarify the etiology of PR and avoid confusion, the collection of different biological materials simultaneously and the usage of CQ RT-PCR as a diagnostic technique are recommended.<\/jats:p>","DOI":"10.3390\/diagnostics14080843","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T10:30:52Z","timestamp":1713436252000},"page":"843","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Association between Pityriasis Rosea (PR) and HHV-6\/HHV-7 Infection: Importance of Sample Selection and Diagnostic Techniques"],"prefix":"10.3390","volume":"14","author":[{"given":"Mine","family":"Aydin Kurc","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Tekirdag Namik Kemal University, Tekirdag 59030, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0000-5568","authenticated-orcid":false,"given":"Gamze","family":"Erfan","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine, Ac\u0131badem University, Istanbul 34752, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayse Demet","family":"Kaya","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Istanbul Okan University, Istanbul 34959, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dumrul","family":"G\u00fclen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Tekirdag Namik Kemal University, Tekirdag 59030, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meltem","family":"Oznur","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine, Tekirdag Namik Kemal University, Tekirdag 59030, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehmet Emin","family":"Yanik","sequence":"additional","affiliation":[{"name":"Clinic of Dermatology, Sancaktepe Region Hospital, Istanbul 34885, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1046\/j.0022-202x.2001.00040.x","article-title":"Skin diseases associated with human herpesvirus 6, 7, and 8 infection","volume":"6","author":"Blauvelt","year":"2001","journal-title":"J. Investig. Dermatol. Symp. Proc."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1159\/000368352","article-title":"Persistent pityriasis rosea: An unusual form of pityriasis rosea with persistent active HHV-6 and HHV-7 infection","volume":"230","author":"Drago","year":"2015","journal-title":"Dermatology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/j.1468-3083.2008.02939.x","article-title":"The role of human herpesvirus 6, human herpesvirus 7, Epstein-Barr virus and cytomegalovirus in the aetiology of pityriasis rosea","volume":"23","author":"Adisen","year":"2009","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.clindermatol.2010.03.005","article-title":"Pityriasis rosea and herpesviruses: Facts and controversies","volume":"28","author":"Rebora","year":"2010","journal-title":"Clin. Dermatol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1016\/j.mayocp.2012.04.010","article-title":"Human herpesviruses 6, 7, and 8 from a dermatologic perspective","volume":"87","author":"Wolz","year":"2012","journal-title":"Mayo Clin. Proc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1111\/j.1365-4632.2005.02007.x","article-title":"Effect on quality of life in patients with pityriasis rosea: Is it associated with rash severity?","volume":"44","author":"Chuh","year":"2005","journal-title":"Int. J. Dermatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1017\/S0950268804002304","article-title":"Pityriasis rosea--evidence for and against an infectious aetiology","volume":"132","author":"Chuh","year":"2004","journal-title":"Epidemiol. Infect."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"728","DOI":"10.18203\/issn.2455-4529.IntJResDermatol20194632","article-title":"Prevalence and molecular characterization of HHV-6 and HHV-7 in patients with pityriasis rosea using polymerase chain reaction in a tertiary care hospital, Puducherry","volume":"5","author":"Murugaiyan","year":"2019","journal-title":"Int. J. Res. Dermatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e20173179","DOI":"10.1542\/peds.2017-3179","article-title":"Relapsing Pityriasis Rosea With HHV-7 Reactivation in an 11-Year-Old Girl","volume":"141","author":"Engelmann","year":"2018","journal-title":"Pediatrics"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"21","DOI":"10.5070\/D3238036020","article-title":"Atypical presentations of pityriasis rosea: A reply","volume":"23","author":"Ciccarese","year":"2017","journal-title":"Dermatol. Online J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.2340\/00015555-3265","article-title":"Commentary on: \u201cPityriasis Rosea Recurrence is Much Higher than Previously Known: A Prospective Study\u201d","volume":"99","author":"Drago","year":"2019","journal-title":"Acta Derm. Venereol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"134","DOI":"10.4103\/ds.ds_33_18","article-title":"The relationship between pityriasis rosea, seasonal factors, and other herpetic infections: A time series analysis","volume":"37","author":"Hayta","year":"2019","journal-title":"Dermatol. Sin."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"29","DOI":"10.4314\/nqjhm.v20i1.57989","article-title":"The pityriasis rosea calendar: A 7 year review of seasonal variation, age and sex distribution","volume":"20","author":"Ayanlowo","year":"2010","journal-title":"Nig. Q. J. Hosp. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"7","DOI":"10.21608\/mjmu.2021.61238.1008","article-title":"Detection of Human Herpes Virus 6&7 in pityriasis rosea and the efficacy of acyclovir","volume":"50","author":"Selim","year":"2021","journal-title":"Mansoura Med. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/s00063-016-0198-0","article-title":"Cytomegalovirus and Epstein-Barr virus reactivation in the intensive care unit","volume":"112","author":"Yazici","year":"2017","journal-title":"Med. Klin. Intensivmed. Notfmed."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"956","DOI":"10.3906\/sag-1802-150","article-title":"Parvovirus B19 seroprevalence in Turkish blood donors","volume":"48","author":"Yenicesu","year":"2018","journal-title":"Turk. J. Med. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"102775","DOI":"10.1016\/j.transci.2020.102775","article-title":"Prevalence and quantity of Parvovirus B19 DNA among blood donors from a Regional Blood Center in Turkey","volume":"59","author":"Yilmaz","year":"2020","journal-title":"Transfus. Apher. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.1002\/jmv.26549","article-title":"Human herpesvirus-6,-7, and Epstein-Barr virus reactivation in pityriasis rosea during COVID-19","volume":"93","author":"Drago","year":"2021","journal-title":"J. Med. Virol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4069","DOI":"10.1002\/jmv.27852","article-title":"Pityriasis rosea, human herpesvirus 6 infection and pregnancy","volume":"94","author":"Drago","year":"2022","journal-title":"J. Med. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s12985-021-01510-6","article-title":"A multiplex real-time PCR quantitation of human herpesvirus-6, 7, 8 viruses: Application in blood transfusions","volume":"18","author":"Zheng","year":"2021","journal-title":"Virol. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1046\/j.1523-1747.2002.00200.x","article-title":"Pityriasis rosea is associated with systemic active infection with both human herpesvirus-7 and human herpesvirus-6","volume":"119","author":"Watanabe","year":"2002","journal-title":"J. Investig. Dermatol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1111\/j.0022-202X.2005.23719.x","article-title":"Additional evidence that pityriasis rosea is associated with reactivation of human herpesvirus-6 and -7","volume":"124","author":"Broccolo","year":"2005","journal-title":"J. Investig. Dermatol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.jaad.2008.07.045","article-title":"Pityriasis rosea: An update with a critical appraisal of its possible herpesviral etiology","volume":"61","author":"Drago","year":"2009","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1159\/000245991","article-title":"Human herpesvirus 7 in patients with pityriasis rosea. Electron microscopy investigations and polymerase chain reaction in mononuclear cells, plasma and skin","volume":"195","author":"Drago","year":"1997","journal-title":"Dermatology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1046\/j.1365-2133.1999.02630.x","article-title":"Reactivation of human herpesvirus 6 in pityriasis rosea","volume":"140","author":"Yasukawa","year":"1999","journal-title":"Br. J. Dermatol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1046\/j.1523-1747.1999.00658.x","article-title":"Human herpesvirus 7 and pityriasis rosea","volume":"113","author":"Watanabe","year":"1999","journal-title":"J. Investig. Dermatol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1097\/01.EWX.0000392820.13832.b7","article-title":"Evaluation of pityriasis rosea associated with human herpesviruses 6 and 7","volume":"8","author":"Mohammed","year":"2011","journal-title":"J. Egypt. Women\u2019s Dermatol. Soc."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/14\/8\/843\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:30:26Z","timestamp":1760106626000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/14\/8\/843"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,18]]},"references-count":27,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["diagnostics14080843"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics14080843","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,18]]}}}