{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T15:45:34Z","timestamp":1789573534525,"version":"build-2803163510"},"reference-count":45,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T00:00:00Z","timestamp":1715731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Helsinki and Uusimaa Hospital District (HUS), Finland","award":["Y1014SULE1"],"award-info":[{"award-number":["Y1014SULE1"]}]},{"name":"Helsinki and Uusimaa Hospital District (HUS), Finland","award":["Y1014SL018"],"award-info":[{"award-number":["Y1014SL018"]}]},{"name":"Helsinki and Uusimaa Hospital District (HUS), Finland","award":["Y1014SL017"],"award-info":[{"award-number":["Y1014SL017"]}]},{"name":"Helsinki and Uusimaa Hospital District (HUS), Finland","award":["TYH2019319"],"award-info":[{"award-number":["TYH2019319"]}]},{"name":"Helsinki and Uusimaa Hospital District (HUS), Finland","award":["TYH2018229"],"award-info":[{"award-number":["TYH2018229"]}]},{"name":"Helsinki and Uusimaa Hospital District (HUS), Finland","award":["TYH2017251"],"award-info":[{"award-number":["TYH2017251"]}]},{"name":"Helsinki and Uusimaa Hospital District (HUS), Finland","award":["TYH2016251"],"award-info":[{"award-number":["TYH2016251"]}]},{"name":"Helsinki and Uusimaa Hospital District (HUS), Finland","award":["TYH2020337"],"award-info":[{"award-number":["TYH2020337"]}]},{"name":"Helsinki and Uusimaa Hospital District (HUS), Finland","award":["TYH2022225"],"award-info":[{"award-number":["TYH2022225"]}]},{"name":"Helsinki and Uusimaa Hospital District (HUS), Finland","award":["Y2519SU010"],"award-info":[{"award-number":["Y2519SU010"]}]},{"name":"Helsinki and Uusimaa Hospital District (HUS), Finland","award":["202231103652"],"award-info":[{"award-number":["202231103652"]}]},{"name":"Finnish Dental Association Apollonia, Finland","award":["Y1014SULE1"],"award-info":[{"award-number":["Y1014SULE1"]}]},{"name":"Finnish Dental Association Apollonia, Finland","award":["Y1014SL018"],"award-info":[{"award-number":["Y1014SL018"]}]},{"name":"Finnish Dental Association Apollonia, Finland","award":["Y1014SL017"],"award-info":[{"award-number":["Y1014SL017"]}]},{"name":"Finnish Dental Association Apollonia, Finland","award":["TYH2019319"],"award-info":[{"award-number":["TYH2019319"]}]},{"name":"Finnish Dental Association Apollonia, Finland","award":["TYH2018229"],"award-info":[{"award-number":["TYH2018229"]}]},{"name":"Finnish Dental Association Apollonia, Finland","award":["TYH2017251"],"award-info":[{"award-number":["TYH2017251"]}]},{"name":"Finnish Dental Association Apollonia, Finland","award":["TYH2016251"],"award-info":[{"award-number":["TYH2016251"]}]},{"name":"Finnish Dental Association Apollonia, Finland","award":["TYH2020337"],"award-info":[{"award-number":["TYH2020337"]}]},{"name":"Finnish Dental Association Apollonia, Finland","award":["TYH2022225"],"award-info":[{"award-number":["TYH2022225"]}]},{"name":"Finnish Dental Association Apollonia, Finland","award":["Y2519SU010"],"award-info":[{"award-number":["Y2519SU010"]}]},{"name":"Finnish Dental Association Apollonia, Finland","award":["202231103652"],"award-info":[{"award-number":["202231103652"]}]},{"name":"Karolinska Institutet, Sweden","award":["Y1014SULE1"],"award-info":[{"award-number":["Y1014SULE1"]}]},{"name":"Karolinska Institutet, Sweden","award":["Y1014SL018"],"award-info":[{"award-number":["Y1014SL018"]}]},{"name":"Karolinska Institutet, Sweden","award":["Y1014SL017"],"award-info":[{"award-number":["Y1014SL017"]}]},{"name":"Karolinska Institutet, Sweden","award":["TYH2019319"],"award-info":[{"award-number":["TYH2019319"]}]},{"name":"Karolinska Institutet, Sweden","award":["TYH2018229"],"award-info":[{"award-number":["TYH2018229"]}]},{"name":"Karolinska Institutet, Sweden","award":["TYH2017251"],"award-info":[{"award-number":["TYH2017251"]}]},{"name":"Karolinska Institutet, Sweden","award":["TYH2016251"],"award-info":[{"award-number":["TYH2016251"]}]},{"name":"Karolinska Institutet, Sweden","award":["TYH2020337"],"award-info":[{"award-number":["TYH2020337"]}]},{"name":"Karolinska Institutet, Sweden","award":["TYH2022225"],"award-info":[{"award-number":["TYH2022225"]}]},{"name":"Karolinska Institutet, Sweden","award":["Y2519SU010"],"award-info":[{"award-number":["Y2519SU010"]}]},{"name":"Karolinska Institutet, Sweden","award":["202231103652"],"award-info":[{"award-number":["202231103652"]}]},{"name":"Swedish Research Council","award":["Y1014SULE1"],"award-info":[{"award-number":["Y1014SULE1"]}]},{"name":"Swedish Research Council","award":["Y1014SL018"],"award-info":[{"award-number":["Y1014SL018"]}]},{"name":"Swedish Research Council","award":["Y1014SL017"],"award-info":[{"award-number":["Y1014SL017"]}]},{"name":"Swedish Research Council","award":["TYH2019319"],"award-info":[{"award-number":["TYH2019319"]}]},{"name":"Swedish Research Council","award":["TYH2018229"],"award-info":[{"award-number":["TYH2018229"]}]},{"name":"Swedish Research Council","award":["TYH2017251"],"award-info":[{"award-number":["TYH2017251"]}]},{"name":"Swedish Research Council","award":["TYH2016251"],"award-info":[{"award-number":["TYH2016251"]}]},{"name":"Swedish Research Council","award":["TYH2020337"],"award-info":[{"award-number":["TYH2020337"]}]},{"name":"Swedish Research Council","award":["TYH2022225"],"award-info":[{"award-number":["TYH2022225"]}]},{"name":"Swedish Research Council","award":["Y2519SU010"],"award-info":[{"award-number":["Y2519SU010"]}]},{"name":"Swedish Research Council","award":["202231103652"],"award-info":[{"award-number":["202231103652"]}]},{"name":"Patent Revenue Fund for Research in Preventive Odontology","award":["Y1014SULE1"],"award-info":[{"award-number":["Y1014SULE1"]}]},{"name":"Patent Revenue Fund for Research in Preventive Odontology","award":["Y1014SL018"],"award-info":[{"award-number":["Y1014SL018"]}]},{"name":"Patent Revenue Fund for Research in Preventive Odontology","award":["Y1014SL017"],"award-info":[{"award-number":["Y1014SL017"]}]},{"name":"Patent Revenue Fund for Research in Preventive Odontology","award":["TYH2019319"],"award-info":[{"award-number":["TYH2019319"]}]},{"name":"Patent Revenue Fund for Research in Preventive Odontology","award":["TYH2018229"],"award-info":[{"award-number":["TYH2018229"]}]},{"name":"Patent Revenue Fund for Research in Preventive Odontology","award":["TYH2017251"],"award-info":[{"award-number":["TYH2017251"]}]},{"name":"Patent Revenue Fund for Research in Preventive Odontology","award":["TYH2016251"],"award-info":[{"award-number":["TYH2016251"]}]},{"name":"Patent Revenue Fund for Research in Preventive Odontology","award":["TYH2020337"],"award-info":[{"award-number":["TYH2020337"]}]},{"name":"Patent Revenue Fund for Research in Preventive Odontology","award":["TYH2022225"],"award-info":[{"award-number":["TYH2022225"]}]},{"name":"Patent Revenue Fund for Research in Preventive Odontology","award":["Y2519SU010"],"award-info":[{"award-number":["Y2519SU010"]}]},{"name":"Patent Revenue Fund for Research in Preventive Odontology","award":["202231103652"],"award-info":[{"award-number":["202231103652"]}]},{"name":"steering group KI\/Region Stockholm for dental research\u2014SOF","award":["Y1014SULE1"],"award-info":[{"award-number":["Y1014SULE1"]}]},{"name":"steering group KI\/Region Stockholm for dental research\u2014SOF","award":["Y1014SL018"],"award-info":[{"award-number":["Y1014SL018"]}]},{"name":"steering group KI\/Region Stockholm for dental research\u2014SOF","award":["Y1014SL017"],"award-info":[{"award-number":["Y1014SL017"]}]},{"name":"steering group KI\/Region Stockholm for dental research\u2014SOF","award":["TYH2019319"],"award-info":[{"award-number":["TYH2019319"]}]},{"name":"steering group KI\/Region Stockholm for dental research\u2014SOF","award":["TYH2018229"],"award-info":[{"award-number":["TYH2018229"]}]},{"name":"steering group KI\/Region Stockholm for dental research\u2014SOF","award":["TYH2017251"],"award-info":[{"award-number":["TYH2017251"]}]},{"name":"steering group KI\/Region Stockholm for dental research\u2014SOF","award":["TYH2016251"],"award-info":[{"award-number":["TYH2016251"]}]},{"name":"steering group KI\/Region Stockholm for dental research\u2014SOF","award":["TYH2020337"],"award-info":[{"award-number":["TYH2020337"]}]},{"name":"steering group KI\/Region Stockholm for dental research\u2014SOF","award":["TYH2022225"],"award-info":[{"award-number":["TYH2022225"]}]},{"name":"steering group KI\/Region Stockholm for dental research\u2014SOF","award":["Y2519SU010"],"award-info":[{"award-number":["Y2519SU010"]}]},{"name":"steering group KI\/Region Stockholm for dental research\u2014SOF","award":["202231103652"],"award-info":[{"award-number":["202231103652"]}]},{"name":"Indonesian Education Scholarship from PUSLAPDIK and LPDP Republic of Indonesia","award":["Y1014SULE1"],"award-info":[{"award-number":["Y1014SULE1"]}]},{"name":"Indonesian Education Scholarship from PUSLAPDIK and LPDP Republic of Indonesia","award":["Y1014SL018"],"award-info":[{"award-number":["Y1014SL018"]}]},{"name":"Indonesian Education Scholarship from PUSLAPDIK and LPDP Republic of Indonesia","award":["Y1014SL017"],"award-info":[{"award-number":["Y1014SL017"]}]},{"name":"Indonesian Education Scholarship from PUSLAPDIK and LPDP Republic of Indonesia","award":["TYH2019319"],"award-info":[{"award-number":["TYH2019319"]}]},{"name":"Indonesian Education Scholarship from PUSLAPDIK and LPDP Republic of Indonesia","award":["TYH2018229"],"award-info":[{"award-number":["TYH2018229"]}]},{"name":"Indonesian Education Scholarship from PUSLAPDIK and LPDP Republic of Indonesia","award":["TYH2017251"],"award-info":[{"award-number":["TYH2017251"]}]},{"name":"Indonesian Education Scholarship from PUSLAPDIK and LPDP Republic of Indonesia","award":["TYH2016251"],"award-info":[{"award-number":["TYH2016251"]}]},{"name":"Indonesian Education Scholarship from PUSLAPDIK and LPDP Republic of Indonesia","award":["TYH2020337"],"award-info":[{"award-number":["TYH2020337"]}]},{"name":"Indonesian Education Scholarship from PUSLAPDIK and LPDP Republic of Indonesia","award":["TYH2022225"],"award-info":[{"award-number":["TYH2022225"]}]},{"name":"Indonesian Education Scholarship from PUSLAPDIK and LPDP Republic of Indonesia","award":["Y2519SU010"],"award-info":[{"award-number":["Y2519SU010"]}]},{"name":"Indonesian Education Scholarship from PUSLAPDIK and LPDP Republic of Indonesia","award":["202231103652"],"award-info":[{"award-number":["202231103652"]}]},{"name":"University of Helsinki","award":["Y1014SULE1"],"award-info":[{"award-number":["Y1014SULE1"]}]},{"name":"University of Helsinki","award":["Y1014SL018"],"award-info":[{"award-number":["Y1014SL018"]}]},{"name":"University of Helsinki","award":["Y1014SL017"],"award-info":[{"award-number":["Y1014SL017"]}]},{"name":"University of Helsinki","award":["TYH2019319"],"award-info":[{"award-number":["TYH2019319"]}]},{"name":"University of Helsinki","award":["TYH2018229"],"award-info":[{"award-number":["TYH2018229"]}]},{"name":"University of Helsinki","award":["TYH2017251"],"award-info":[{"award-number":["TYH2017251"]}]},{"name":"University of Helsinki","award":["TYH2016251"],"award-info":[{"award-number":["TYH2016251"]}]},{"name":"University of Helsinki","award":["TYH2020337"],"award-info":[{"award-number":["TYH2020337"]}]},{"name":"University of Helsinki","award":["TYH2022225"],"award-info":[{"award-number":["TYH2022225"]}]},{"name":"University of Helsinki","award":["Y2519SU010"],"award-info":[{"award-number":["Y2519SU010"]}]},{"name":"University of Helsinki","award":["202231103652"],"award-info":[{"award-number":["202231103652"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background: This study investigated in vivo regulation and levels of active matrix metalloproteinase-8 (aMMP-8), a major collagenolytic protease, in periodontitis. Methods: Twenty-seven adults with chronic periodontitis (CP) and 30 periodontally healthy controls (HC) were enrolled in immunohistochemistry and transcriptomics analytics in order to assess Treponema denticola (Td) dentilisin and MMP-8 immunoexpression, mRNA expression of MMP-8 and its regulators (IL-1\u03b2, MMP-2, MMP-7, TIMP-1). Furthermore, the periodontal anti-infective treatment effect was monitored by four different MMP-8 assays (aMMP-8-IFMA, aMMP-8-Oralyzer, MMP-8-activity [RFU\/minute], and total MMP-8 by ELISA) among 12 CP (compared to 25 HC). Results: Immunohistochemistry revealed significantly more Td-dentilisin and MMP-8 immunoreactivities in CP vs. HC. Transcriptomics revealed significantly elevated IL-1\u03b2 and MMP-7 RNA expressions, and MMP-2 RNA was slightly reduced. No significant differences were recorded in the relatively low or barely detectable levels of MMP-8 mRNAs. Periodontal treatment significantly decreased all MMP-8 assay levels accompanied by the assessed clinical indices (periodontal probing depths, bleeding-on-probing, and visual plaque levels). However, active but not total MMP-8 levels persisted higher in CP than in periodontally healthy controls. Conclusion: In periodontal health, there are low aMMP-8 levels. The presence of Td-dentilisin in CP gingivae is associated with elevated aMMP-8 levels, potentially contributing to a higher risk of active periodontal tissue collagenolysis and progression of periodontitis. This can be detected by aMMP-8-specific assays and online\/real-time aMMP-8 chair-side testing.<\/jats:p>","DOI":"10.3390\/diagnostics14101011","type":"journal-article","created":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T03:35:55Z","timestamp":1715744155000},"page":"1011","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["In Vivo Regulation of Active Matrix Metalloproteinase-8 (aMMP-8) in Periodontitis: From Transcriptomics to Real-Time Online Diagnostics and Treatment Monitoring"],"prefix":"10.3390","volume":"14","author":[{"given":"Nur Rahman Ahmad Seno","family":"Aji","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, Finland"},{"name":"Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada, Jalan Denta No. 1 Sekip Utara, 10 Sleman, Yogyakarta 55281, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0129-8809","authenticated-orcid":false,"given":"T\u00fclay","family":"Yucel-Lindberg","sequence":"additional","affiliation":[{"name":"Division of Pediatric Dentistry, Department of Dental Medicine, Karolinska Institutet, 171 77 Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5821-5299","authenticated-orcid":false,"given":"Ismo T.","family":"R\u00e4is\u00e4nen","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi","family":"Kuula","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6427-3196","authenticated-orcid":false,"given":"Mikko T.","family":"Nieminen","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, Finland"},{"name":"Department of Otorhinolaryngology\u2014Head and Neck Surgery, Helsinki University Hospital and University of Helsinki, 00290 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mael\u00edosa T. C.","family":"Mc Crudden","sequence":"additional","affiliation":[{"name":"Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine Dentistry and Biomedical Science, Queen\u2019s University Belfast, Belfast BT9 7BL, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dyah","family":"Listyarifah","sequence":"additional","affiliation":[{"name":"Department of Dental Biomedical Sciences, Faculty of Dentistry, Universitas Gadjah Mada, Jl. Denta Sekip Utara No 1, Yogyakarta 55281, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Lundmark","sequence":"additional","affiliation":[{"name":"Division of Pediatric Dentistry, Department of Dental Medicine, Karolinska Institutet, 171 77 Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3150-1150","authenticated-orcid":false,"given":"Fionnuala T.","family":"Lundy","sequence":"additional","affiliation":[{"name":"Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine Dentistry and Biomedical Science, Queen\u2019s University Belfast, Belfast BT9 7BL, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2097-2459","authenticated-orcid":false,"given":"Shipra","family":"Gupta","sequence":"additional","affiliation":[{"name":"Oral Health Sciences Centre, Post Graduate Institute of Medical Education & Research, Chandigarh 160012, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"Sorsa","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, Finland"},{"name":"Division of Oral Diseases, Department of Dental Medicine, Karolinska Institutet, 171 77 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S173","DOI":"10.1002\/JPER.17-0721","article-title":"Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions","volume":"89","author":"Papapanou","year":"2018","journal-title":"J. Periodontol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1099\/mic.0.055939-0","article-title":"Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities","volume":"158","author":"Cogoni","year":"2012","journal-title":"Microbiology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1590\/1678-775720140259","article-title":"Host response mechanisms in periodontal diseases","volume":"23","author":"Silva","year":"2015","journal-title":"J. Appl. Oral Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"13","DOI":"10.3934\/molsci.2021002","article-title":"A Mini Review on the Associations of Matrix Metalloproteinases (MMPs) -1, -8, -13 with Periodontal Disease","volume":"8","author":"Khuda","year":"2021","journal-title":"AIMS Mol. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1111\/prd.12331","article-title":"Current understanding of periodontal disease pathogenesis and targets for host-modulation therapy","volume":"84","author":"Hajishengallis","year":"2020","journal-title":"Periodontology 2000"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"17038","DOI":"10.1038\/nrdp.2017.38","article-title":"Periodontal diseases","volume":"3","author":"Kinane","year":"2017","journal-title":"Nat. Rev. Dis. Primers."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.pmbts.2017.02.005","article-title":"Biochemical and Biological Attributes of Matrix Metalloproteinases","volume":"147","author":"Cui","year":"2017","journal-title":"Prog. Mol. Biol. Transl. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1023\/A:1026028303196","article-title":"Regulation of matrix metalloproteinases: An overview","volume":"253","author":"Chakraborti","year":"2003","journal-title":"Mol. Cell. Biochem."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Sorsa, T., Alassiri, S., Grigoriadis, A., R\u00e4is\u00e4nen, I.T., P\u00e4rn\u00e4nen, P., Nwhator, S.O., Gieselmann, D.-R., and Sakellari, D. (2020). Active MMP-8 (aMMP-8) as a Grading and Staging Biomarker in the Periodontitis Classification. Diagnostics, 10.","DOI":"10.3390\/diagnostics10020061"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Keskin, M., Rintamarttunen, J., G\u00fcl\u00e7i\u00e7ek, E., R\u00e4is\u00e4nen, I.T., Gupta, S., Tervahartiala, T., P\u00e4til\u00e4, T., and Sorsa, T. (2023). A Comparative Analysis of Treatment-Related Changes in the Diagnostic Biomarker Active Metalloproteinase-8 Levels in Patients with Periodontitis. Diagnostics, 13.","DOI":"10.3390\/diagnostics13050903"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1111\/jcpe.13188","article-title":"Accuracy of single molecular bi-omarkers in gingival crevicular fluid for the diagnosis of periodontitis: A systematic review and metaanalysis","volume":"46","author":"Nibali","year":"2019","journal-title":"J. Clin. Periodontol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Atanasova, T., Stankova, T., Bivolarska, A., and Vlaykova, T. (2023). Matrix Metalloproteinases in Oral Health\u2014Special Attention on MMP-8. Biomedicines, 11.","DOI":"10.3390\/biomedicines11061514"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/jcpe.13202","article-title":"Accuracy of single molecular bi-omarkers in saliva for the diagnosis of periodontitis: A systematic review and meta-analysis","volume":"47","author":"Nibali","year":"2020","journal-title":"J. Clin. Periodontol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Alassy, H., Parachuru, P., and Wolff, L. (2019). Peri-Implantitis Diagnosis and Prognosis Using Biomarkers in Peri-Implant Crevicular Fluid: A Narrative Review. Diagnostics, 9.","DOI":"10.3390\/diagnostics9040214"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1111\/jcpe.13485","article-title":"Diagnostic accuracy of a point-of-care aMMP-8 test in the discrimination of periodontal health and disease","volume":"48","author":"Deng","year":"2021","journal-title":"J. Clin. Periodontol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1111\/jre.12999","article-title":"Diagnostic accuracy of active matrix metalloproteinase-8 point-of-care test for the discrimination of periodontal health status: Comparison of saliva and oral rinse samples","volume":"57","author":"Deng","year":"2022","journal-title":"J. Periodontal Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"11421","DOI":"10.1016\/S0021-9258(19)38413-3","article-title":"Human neutrophil collagenase. A distinct gene product with homology to other matrix metalloproteinases","volume":"265","author":"Pourmotabbed","year":"1990","journal-title":"J. Biol. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1182\/blood.V77.12.2731.2731","article-title":"Structure and expression of the cDNA encoding human neutrophil colla-genase","volume":"77","author":"Devarajan","year":"1991","journal-title":"Blood"},{"key":"ref_19","unstructured":"Rawlings, N.D., and Salvesen, G. (2013). Handbook of Proteolytic Enzymes, Academic Press. [3rd ed.]. Available online: https:\/\/www.sciencedirect.com\/science\/article\/pii\/B9780123822192001538."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1986","DOI":"10.1177\/00220345960750121101","article-title":"Membrane Components of Treponema denticola Trigger Proteinase Release from Human Polymorphonuclear Leukocytes","volume":"75","author":"Ding","year":"1996","journal-title":"J. Dent. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1056\/NEJM198902093200606","article-title":"Tissue destruction by neutrophils","volume":"320","author":"Weiss","year":"1989","journal-title":"N. Engl. J. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"11023","DOI":"10.1074\/jbc.271.18.11023","article-title":"Chondrocyte Matrix Metalloproteinase-8: HUMAN ARTICULAR CHONDROCYTES EXPRESS NEUTROPHIL COLLAGENASE","volume":"271","author":"Cole","year":"1996","journal-title":"J. Biol. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Deng, K., Zonta, F., Yang, H., Pelekos, G., and Tonetti, M.S. (2023). Development of a machine learning multiclass screening tool for per-iodontal health status based on non-clinical parameters and salivary biomarkers. J. Clin. Periodontol.","DOI":"10.1111\/jcpe.13856"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1111\/jop.12095","article-title":"IL-17C and its receptor IL-17RA\/IL-17RE identify human oral epithelial cell as an inflammatory cell in recurrent aphthous ulcer","volume":"43","author":"Kouri","year":"2014","journal-title":"J. Oral Pathol. Med."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Verhulst, M.J.L., Teeuw, W.J., Bizzarro, S., Muris, J., Su, N., Nicu, E.A., Nazmi, K., Bikker, F.J., and Loos, B.G. (2019). A rapid, non-invasive tool for periodontitis screening in a medical care setting. BMC Oral Health, 19.","DOI":"10.1186\/s12903-019-0784-7"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"18475","DOI":"10.1038\/srep18475","article-title":"Transcriptome analysis reveals mucin 4 to be highly associated with periodontitis and identifies pleckstrin as a link to systemic diseases","volume":"5","author":"Lundmark","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_27","first-page":"556","article-title":"Matrix metalloproteinase-8 activity in gingival crevicular fluid: Development of a novel assay","volume":"52","author":"Irwin","year":"2016","journal-title":"J. Periodontal Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1111\/jop.12729","article-title":"Treponema denticola chymotrypsin-like proteinase is present in early-stage mobile tongue squamous cell carcinoma and related to the clinicopathological features","volume":"47","author":"Listyarifah","year":"2018","journal-title":"J. Oral Pathol. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"23959","DOI":"10.1074\/jbc.273.37.23959","article-title":"Collagenase 2 (MMP-8) expression in murine tissue-remodeling processes. Analysis of its potential role in postpartum involution of the uterus","volume":"273","author":"Fueyo","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1969","DOI":"10.1177\/00220345000790120801","article-title":"The in vivo expression of the collagenolytic matrix metalloproteinases (MMP-2, -8, -13, and -14) and matrilysin (MMP-7) in adult and localized juvenile periodontitis","volume":"79","author":"Tervahartiala","year":"2000","journal-title":"J. Dent. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1111\/j.1600-0765.1993.tb01079.x","article-title":"Expression of TIMP-1, TIMP-2 and collagenase mRNA in periodontitis-affected human gingival tissue","volume":"28","author":"Nomura","year":"1993","journal-title":"J. Periodontal Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1111\/j.1600-0765.1993.tb02114.x","article-title":"Detection of interleukin-8 and matrix metalloproteinases transcripts in healthy and diseased gingival biopsies by RNA\/PCR","volume":"28","author":"Tonetti","year":"1993","journal-title":"J. Periodontal Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1111\/j.1600-0722.1996.tb00142.x","article-title":"Matrix metalloproteinases-1 and -8 and TIMP-1 mRNA levels in normal and diseased human gingivae","volume":"104","author":"Aiba","year":"1996","journal-title":"Eur. J. Oral Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4205","DOI":"10.1007\/s00784-019-02861-9","article-title":"Expression of Toll-like receptors 2 and 4 and its association with matrix metalloproteinases in symptomatic and asymptomatic apical periodontitis","volume":"23","author":"Astorga","year":"2019","journal-title":"Clin. Oral Investig."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1038\/s41415-024-7138-9","article-title":"Point of care technology for screening and referrals","volume":"236","author":"Sahni","year":"2024","journal-title":"Br. Dent. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/j.1600-0765.1990.tb00914.x","article-title":"Identification of polymorphonuclear leukocyte collagenase and gelatinase activities in mouthrinse samples: Correlation with periodontal disease activity in adult and juvenile periodontitis","volume":"25","author":"Gangbar","year":"1990","journal-title":"J. Periodontal Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4687","DOI":"10.1128\/iai.59.12.4687-4692.1991","article-title":"Evidence for polymorphonuclear leukocyte collagenase and 92-kilodalton gelatinase in gingival crevicular fluid","volume":"59","author":"Overall","year":"1991","journal-title":"Infect. Immun."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/j.1600-0765.1995.tb01249.x","article-title":"Evidence of a direct relationship between neutrophil collagenase activity and periodontal tissue destruction in vivo: Role of active enzyme in human periodontitis","volume":"30","author":"Lee","year":"1995","journal-title":"J. Periodontal Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1902\/jop.1999.70.11.1292","article-title":"Assessment of a novel screening test for neutrophil collagenase activity in the diagnosis of periodontal diseases","volume":"70","author":"Mancini","year":"1999","journal-title":"J. Periodontol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2319","DOI":"10.1128\/IAI.67.5.2319-2326.1999","article-title":"Activation of neutrophil collagenase in periodontitis","volume":"67","author":"Romanelli","year":"1999","journal-title":"Infect. Immun."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1111\/prd.12315","article-title":"Periodontal therapeutics: Current host-modulation agents and future directions","volume":"82","author":"Golub","year":"2020","journal-title":"Periodontology 2000"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/j.adaj.2022.03.005","article-title":"Precision periodontics: Quantitative measures of disease progression","volume":"153","author":"Gellibolian","year":"2022","journal-title":"J. Am. Dent. Assoc."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1111\/jcpe.12229","article-title":"Sulcus fluid bone marker levels and the outcome of surgical treatment of peri-implantitis","volume":"41","author":"Wohlfahrt","year":"2014","journal-title":"J. Clin. Periodontol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1111\/clr.12708","article-title":"Protein biomarkers and microbial profiles in peri-implantitis","volume":"27","author":"Wang","year":"2016","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s10266-019-00435-5","article-title":"Relationship between TNF-\u03b1, MMP-8, and MMP-9 levels in gingival crevicular fluid and the subgingival microbiota in periodontal disease","volume":"108","year":"2020","journal-title":"Odontology"}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/14\/10\/1011\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:42:30Z","timestamp":1760107350000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/14\/10\/1011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,15]]},"references-count":45,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["diagnostics14101011"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics14101011","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,15]]}}}