{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T14:16:24Z","timestamp":1794060984466,"version":"4.8.0"},"reference-count":53,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T00:00:00Z","timestamp":1715731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Nurturing R&amp;D Project through the Korea Health Industry Development Institute (KHIDI)","award":["HR22C1605"],"award-info":[{"award-number":["HR22C1605"]}],"id":[{"id":"https:\/\/ror.org\/00fdzyk40","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>(1) Background: Non-invasive prenatal testing (NIPT) is a screening test for fetal aneuploidy using cell-free fetal DNA. The fetal fragments (FF) of cell-free DNA (cfDNA) are derived from apoptotic trophoblast of the placenta. The level of fetal cfDNA is known to be influenced by gestational age, multiple pregnancies, maternal weight, and height. (2) Methods: This study is a single-center retrospective observational study which examines the relationship between the fetal fraction (FF) of cell-free DNA in non-invasive prenatal testing (NIPT) and adverse pregnancy outcomes in singleton pregnancies. A total of 1393 samples were collected between 10 weeks and 6 days, and 25 weeks and 3 days of gestation. (3) Results: Hypertensive disease of pregnancy (HDP) occurred more frequently in the low FF group than the normal FF group (5.17% vs. 1.91%, p = 0.001). Although the rates of small for gestational age (SGA) and placental abruption did not significantly differ between groups, the composite outcome was significantly higher in the low FF group (7.76% vs. 3.64%, p = 0.002). Furthermore, women who later experienced complications such as HDP or gestational diabetes mellitus (GDM) had significantly lower plasma FF levels compared to those without complications (p &lt; 0.001). After adjustments, the low FF group exhibited a significantly higher likelihood of placental compromise (adjusted odds ratio: 1.946). (4) Conclusions: Low FF in NIPT during the first and early second trimesters is associated with adverse pregnancy outcomes, particularly HDP, suggesting its potential as a predictive marker for such outcomes.<\/jats:p>","DOI":"10.3390\/diagnostics14101020","type":"journal-article","created":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T11:31:52Z","timestamp":1715772712000},"page":"1020","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Association between Low Fetal Fraction of Non-Invasive Prenatal Testing and Adverse Pregnancy Outcomes for Placental Compromise"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1118-4920","authenticated-orcid":false,"given":"Soo-Hyun","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul 06135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You-Mi","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul 06135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6974-0301","authenticated-orcid":false,"given":"Ji-Eun","family":"Park","sequence":"additional","affiliation":[{"name":"Center for Genome Diagnostics, CHA Biotech Inc., Seoul 06125, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung-Shin","family":"Shim","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul 06135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0538-3517","authenticated-orcid":false,"given":"Hee-Jin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul 06135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeon-Kyung","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul 06135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1729-0959","authenticated-orcid":false,"given":"June-Seek","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul 06135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joong-Sik","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul 06135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0710-2582","authenticated-orcid":false,"given":"Hyun-Mee","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam 13497, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moon-Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul 06135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0722-1714","authenticated-orcid":false,"given":"Dong-Hyun","family":"Cha","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul 06135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4087-9198","authenticated-orcid":false,"given":"You-Jung","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul 06135, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1056\/NEJMoa1407349","article-title":"Cell-free DNA analysis for noninvasive examination of trisomy","volume":"372","author":"Norton","year":"2015","journal-title":"N. Engl. J. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.cca.2018.08.018","article-title":"Not all chromosome aberrations can be detected by NIPT in women at advanced maternal age: A multicenter retrospective study","volume":"486","author":"Chen","year":"2018","journal-title":"Clin. Chim. Acta"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/S0140-6736(97)02174-0","article-title":"Presence of fetal DNA in maternal plasma and serum","volume":"350","author":"Lo","year":"1997","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1093\/clinchem\/48.3.421","article-title":"Predominant hematopoietic origin of cell-free DNA in plasma and serum after sex mismatched bone marrow transplantation","volume":"48","author":"Lui","year":"2002","journal-title":"Clin. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.cell.2015.11.050","article-title":"Cell-free DNA comprises an in vivo nucleosome footprint that informs its tissues of origin","volume":"164","author":"Snyder","year":"2016","journal-title":"Cell"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1093\/humrep\/deh117","article-title":"Circulating cell-free fetal DNA in maternal serum appears to originate from cyto- and syncytiotrophoblastic cells. Case report","volume":"19","author":"Flori","year":"2004","journal-title":"Hum. Reprod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1002\/pd.1700","article-title":"Free fetal DNA in maternal plasma in anembryonic pregnancies: Confirmation that the origin is the trophoblast","volume":"27","author":"Alberry","year":"2007","journal-title":"Prenat. Diagn."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"S19","DOI":"10.1517\/14712598.2012.670632","article-title":"Non-invasive prenatal diagnosis of fetal aneuploidies using massively parallel sequencing-by ligation and evidence that cell-free fetal DNA in the maternal plasma originates from cytotrophoblastic cells","volume":"12","author":"Faas","year":"2012","journal-title":"Expert Opin. Biol. Ther."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1002\/pd.4126","article-title":"The impact of maternal plasma DNA fetal fraction on next generation sequencing tests for common fetal aneuploidies","volume":"33","author":"Canick","year":"2013","journal-title":"Prenat. Diagn."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1002\/uog.14715","article-title":"Performance of non-invasive prenatal testing when fetal cell-free DNA is absent","volume":"45","author":"Takoudes","year":"2015","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1002\/uog.17484","article-title":"Analysis of cell-free DNA in maternal blood in screening for aneuploidies: Updated meta-analysis","volume":"50","author":"Gil","year":"2017","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1373\/clinchem.2013.207951","article-title":"Circulating fetal cell free DNA fractions differ in autosomal aneuploidies and monosomy X","volume":"60","author":"Rava","year":"2014","journal-title":"Clin. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1002\/uog.15851","article-title":"Screening for trisomies by cell-free DNA testing of maternal blood: Consequences of a failed result","volume":"47","author":"Revello","year":"2016","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1177\/1933719115584445","article-title":"Effects of maternal and fetal characteristics on cell-free fetal DNA fraction in maternal plasma","volume":"22","author":"Zhou","year":"2015","journal-title":"Reprod. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1002\/pd.4541","article-title":"Circulating cell free DNA testing: Are some test failures informative?","volume":"35","author":"Palomaki","year":"2015","journal-title":"Prenat. Diagn."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1002\/pd.4625","article-title":"Factors affecting levels of circulating cell-free fetal DNA in maternal plasma and their implications for noninvasive prenatal testing","volume":"35","author":"Kinnings","year":"2015","journal-title":"Prenat. Diagn."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1002\/uog.12331","article-title":"Fetal fraction in maternal plasma cell-free DNA at 11-13 weeks\u2019 gestation: Relation to maternal and fetal characteristics","volume":"41","author":"Ashoor","year":"2013","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_18","first-page":"937","article-title":"Cell Free DNA fetal fraction in twin gestations in single nucleotide polymorphism-based non-invasive prenatal screening","volume":"8","author":"Hedriana","year":"2019","journal-title":"Prenat. Diagn."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1002\/uog.20290","article-title":"First trimester screening for trisomies by cfDNA testing of maternal blood in singleton and twin pregnancies: Factors affecting test failure","volume":"53","author":"Galeva","year":"2019","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1002\/pd.5620","article-title":"Fetal fraction and noninvasive prenatal testing: What clinicians need to know","volume":"40","author":"Hui","year":"2020","journal-title":"Prenat. Diagn."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1038\/oby.2012.138","article-title":"Increased death of adipose cells, a path to release cell-free DNA into systemic circulation of obese women","volume":"20","author":"Haghiac","year":"2012","journal-title":"Obesity"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1111\/aogs.13848","article-title":"Noninvasive prenatal testing and maternal obesity: A review","volume":"99","author":"Juul","year":"2020","journal-title":"Acta Obstet. Gynecol. Scand."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1002\/uog.13418","article-title":"Repeated failed noninvasive prenatal testing owing to low cell-free fetal DNA fraction and increased variance in a woman with severe autoimmune disease","volume":"44","author":"Hui","year":"2014","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"bcr2016216593","DOI":"10.1136\/bcr-2016-216593","article-title":"Repeated failed non-invasive prenatal testing in a woman with immune thrombocytopenia and antiphospholipid syndrome: Lessons learnt","volume":"2016","author":"Hui","year":"2016","journal-title":"BMJ Case Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1002\/pd.5152","article-title":"The association between anticoagulation therapy, maternal characteristics, and a failed cfDNA test due to a low fetal fraction","volume":"37","author":"Burns","year":"2017","journal-title":"Prenat. Diagn."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1002\/uog.17473","article-title":"Low-molecular-weight heparin associated with reduced fetal fraction and subsequent false-negative cell-free DNA test result for trisomy 21","volume":"51","author":"Ma","year":"2018","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"S64","DOI":"10.1016\/j.placenta.2013.11.014","article-title":"Cell-free fetal DNA in the maternal circulation as an indication of placental health and disease","volume":"35","author":"Taglauer","year":"2014","journal-title":"Placenta"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.cca.2018.12.001","article-title":"Associations of serum markers screening for Down\u2019s syndrome with pregnancy outcomes: A Chinese retrospective cohort study","volume":"489","author":"Yuan","year":"2019","journal-title":"Clin. Chim. Acta"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.ejogrb.2014.07.018","article-title":"Predictive value of combined serum biomarkers for adverse pregnancy outcomes","volume":"181","author":"Cohen","year":"2014","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1159\/000236355","article-title":"Total and fetal cell-free DNA analysis in maternal blood as markers of placental insufficiency in intrauterine growth restriction","volume":"26","author":"Desbriere","year":"2009","journal-title":"Fetal Diagn. Ther."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.ajog.2004.12.023","article-title":"High levels of fetal cell-free DNA in maternal serum: A risk factor for spontaneous preterm delivery","volume":"193","author":"Farina","year":"2005","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"231.e1","DOI":"10.1016\/j.ajog.2016.02.009","article-title":"Cell free DNA fetal fraction and preterm birth","volume":"215","author":"Dugoff","year":"2016","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1111\/1471-0528.15006","article-title":"Implications of failure to achieve a result from prenatal maternal serum cell-free DNA testing: A historical cohort study","volume":"125","author":"Chan","year":"2018","journal-title":"BJOG"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1055\/s-0039-1700860","article-title":"Low fetal fraction and birth weight in women with negative first-trimester cell-free DNA screening","volume":"37","author":"Clapp","year":"2020","journal-title":"Am. J. Perinat."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.preghy.2019.04.002","article-title":"Low fetal fraction of cell-free DNA predicts placental dysfunction and hypertensive disease in pregnancy","volume":"16","author":"Gerson","year":"2019","journal-title":"Pregnancy Hypertens."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1002\/pd.4779","article-title":"Adverse perinatal outcomes are more frequent in pregnancies with a low fetal fraction result on noninvasive prenatal testing","volume":"36","author":"Krishna","year":"2016","journal-title":"Prenat. Diagn."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.preghy.2020.07.015","article-title":"Association between low fetal fraction of cell free DNA at the early second-trimester and adverse pregnancy outcomes","volume":"22","author":"Yuan","year":"2020","journal-title":"Pregnancy Hypertens."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/0029-7844(95)00386-X","article-title":"A United States national reference for fetal growth","volume":"87","author":"Alexander","year":"1996","journal-title":"Obstet. Gynecol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1016\/S0002-9440(10)64127-2","article-title":"Human trophoblast invasion and spiral artery transformation: The role of PECAM-1 in normal pregnancy, preeclampsia, and fetal growth restriction","volume":"158","author":"Lyall","year":"2001","journal-title":"Am. J. Pathol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1002\/uog.18993","article-title":"Association between fetal fraction on cell-free DNA testing and first trimester markers for pre-eclampsia","volume":"52","author":"Rolnik","year":"2018","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1002\/pd.1513","article-title":"A prospective analysis of cell-free fetal DNA concentration in maternal plasma as an indicator for adverse pregnancy outcome","volume":"26","author":"Bauer","year":"2006","journal-title":"Prenat. Diagn."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1159\/000346806","article-title":"Maternal plasma cell-free fetal and maternal DNA at 11\u201313 weeks\u2019 gestation: Relation to fetal and maternal characteristics and pregnancy outcomes","volume":"33","author":"Poon","year":"2013","journal-title":"Fetal Diagn Ther."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1002\/uog.14671","article-title":"Maternal plasma cell-free DNA in the prediction of preeclampsia","volume":"45","author":"Rolnik","year":"2015","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2141","DOI":"10.1093\/clinchem\/48.12.2141","article-title":"Fetal DNA clearance from maternal plasma is impaired in preeclampsia","volume":"48","author":"Lau","year":"2002","journal-title":"Clin. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1002\/pd.6028","article-title":"Association between low fetal fraction in cell-free DNA testing and adverse pregnancy outcome: A systematic review","volume":"41","author":"Scgeffer","year":"2021","journal-title":"Prenat. Diagn."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1002\/pd.6034","article-title":"Low fetal fraction in cell-free DNA testing is associated with adverse pregnancy outcome: Analysis of a subcohort of the TRIDENT-2 study","volume":"41","author":"Becking","year":"2021","journal-title":"Prenat. Diagn."},{"key":"ref_47","first-page":"219","article-title":"Maternal and fetal factors affecting cell-free fetal DNA (cffDNA) fraction: A systematic review","volume":"24","author":"Shafiee","year":"2023","journal-title":"J. Reprod. Infertil."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1093\/humrep\/dey033","article-title":"Cell-free fetal DNA testing in singleton IVF conceptions","volume":"33","author":"Lee","year":"2018","journal-title":"Hum. Reprod."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"212.e1","DOI":"10.1016\/j.ajog.2015.03.032","article-title":"Angiogenic markers in pregnancies conceived through in vitro fertilization","volume":"213","author":"Lee","year":"2015","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1007\/s40291-018-0353-9","article-title":"Cell-free DNA fetal fraction in early- and late-onset fetal growth restriction","volume":"22","author":"Morano","year":"2018","journal-title":"Mol. Diagn. Ther."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1002\/pd.3917","article-title":"High levels of fetal DNA are associated with increased risk of spontaneous preterm delivery","volume":"32","author":"Jakobsen","year":"2012","journal-title":"Prenat. Diagn."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1002\/uog.14666","article-title":"Fetal fraction of cell-free DNA in maternal plasma in the prediction of spontaneous preterm delivery","volume":"45","author":"Quezada","year":"2015","journal-title":"Ultrasound Obstst. Gynecol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1080\/14737159.2023.2217331","article-title":"Association between the first and second-trimester cfDNA fetal fraction and spontaneous preterm birth","volume":"23","author":"Luo","year":"2023","journal-title":"Expert Rev. Mol. Diagn."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/14\/10\/1020\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:42:52Z","timestamp":1760107372000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/14\/10\/1020"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,15]]},"references-count":53,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["diagnostics14101020"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics14101020","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,15]]}}}