{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T23:59:08Z","timestamp":1793404748446,"version":"4.5.0"},"reference-count":42,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T00:00:00Z","timestamp":1715817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Research Council of Thailand","award":["NAT6305188e"],"award-info":[{"award-number":["NAT6305188e"]}]},{"name":"the National Research Council of Thailand","award":["PHA6704048S"],"award-info":[{"award-number":["PHA6704048S"]}]},{"name":"the Faculty of Pharmaceutical Sciences, Prince of Songkla University, Thailand","award":["NAT6305188e"],"award-info":[{"award-number":["NAT6305188e"]}]},{"name":"the Faculty of Pharmaceutical Sciences, Prince of Songkla University, Thailand","award":["PHA6704048S"],"award-info":[{"award-number":["PHA6704048S"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Early diagnosis is essential for the successful management of Burkholderia pseudomallei infection, but it cannot be achieved by the current gold standard culture technique. Therefore, this study aimed to develop a lateral flow immunoassay (LFIA) targeting B. pseudomallei capsular polysaccharide. The development was performed by varying nitrocellulose membrane reaction pads and chase buffers. The prototype LFIA is composed of Unisart CN95 and chase buffer containing tris-base, casein, and Surfactant 10G. The assay showed no cross-reactivity with E. coli, S. aureus, P. aeruginosa, and P. acne. The limit of detections (LODs) of the prototype LFIA was 107 and 106 CFU\/mL B. pseudomallei in hemoculture medium and artificial urine, respectively. These LODs suggest that this prototype can detect melioidosis from positive hemoculture bottles but not straight from urine. Additionally, these LODs are still inferior compared to Active Melioidosis Detect (AMDTM). Overall, this prototype holds the potential to be used clinically with hemoculture bottles. However, further improvements should be considered, especially for use with urine samples.<\/jats:p>","DOI":"10.3390\/diagnostics14101033","type":"journal-article","created":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T11:26:17Z","timestamp":1715858777000},"page":"1033","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Development of an Antigen Capture Lateral Flow Immunoassay for the Detection of Burkholderia pseudomallei"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3294-1975","authenticated-orcid":false,"given":"Teerapat","family":"Nualnoi","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai 90110, Songkhla, Thailand"},{"name":"Drug Delivery System Excellence Center (DDSEC), Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai 90110, Songkhla, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5112-0913","authenticated-orcid":false,"given":"Paweena","family":"Wongwitwichot","sequence":"additional","affiliation":[{"name":"Drug Delivery System Excellence Center (DDSEC), Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai 90110, Songkhla, Thailand"},{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai 90110, Songkhla, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siriluk","family":"Kaewmanee","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, Prince of Songkla University, Hat Yai 90110, Songkhla, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pornchanan","family":"Chanchay","sequence":"additional","affiliation":[{"name":"Proteodiag Co., Ltd., Mueang Songkhla 90000, Songkhla, 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Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.tim.2023.07.008","article-title":"Burkholderia pseudomallei.","volume":"32","author":"Phillips","year":"2023","journal-title":"Trends Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1097\/QCO.0000000000000827","article-title":"Drivers of Melioidosis Endemicity: Epidemiological Transition, Zoonosis, and Climate Change","volume":"35","author":"Birnie","year":"2022","journal-title":"Curr. 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