{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:05:04Z","timestamp":1760144704620,"version":"build-2065373602"},"reference-count":33,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T00:00:00Z","timestamp":1715904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute Of Allergy and Infectious Diseases of the National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI164376"],"award-info":[{"award-number":["R21AI164376"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Urine filtration microscopy (UFM) lacks sensitivity in detecting low-intensity Schistosoma haematobium infections. In pursuit of a superior alternative, this study evaluated the performance of FlukeCatcher microscopy (FCM) at detecting S. haematobium eggs in human urine samples. Urine samples were collected from 572 school-age children in Afar, Ethiopia in July 2023 and examined using UFM and FCM approaches. Using the combined UFM and FCM results as a reference, the sensitivity, negative predictive value, and agreement levels for the two testing methods in detecting S. haematobium eggs in urine samples were calculated. The sensitivity and negative predictive value of detecting S. haematobium eggs in urine samples for FCM was 84% and 97%, respectively, compared to 65% and 93% for UFM. The FCM test results had an agreement of 61% with the UFM results, compared to 90% with the combined results of FCM and UFM. However, the average egg count estimates were lower when using FCM (6.6 eggs per 10 mL) compared to UFM (14.7 eggs per 10 mL) (p &lt; 0.0001). Incorporating FCM into specimen processing could improve the diagnosis of S. haematobium infection but may underperform in characterizing the intensity of infection.<\/jats:p>","DOI":"10.3390\/diagnostics14101037","type":"journal-article","created":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T06:53:49Z","timestamp":1715928829000},"page":"1037","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluating the Performance of FlukeCatcher at Detecting Urogenital Schistosomiasis"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9730-5846","authenticated-orcid":false,"given":"Louis","family":"Fok","sequence":"first","affiliation":[{"name":"Department of Epidemiology, University of Nebraska Medical Center, Omaha, NE 68198, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Berhanu","family":"Erko","sequence":"additional","affiliation":[{"name":"Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Brett-Major","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, University of Nebraska Medical Center, Omaha, NE 68198, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abebe","family":"Animut","sequence":"additional","affiliation":[{"name":"Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Jana","family":"Broadhurst","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisy","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, University of Nebraska Medical Center, Omaha, NE 68198, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Linville","sequence":"additional","affiliation":[{"name":"Department of Environmental, Agricultural & Occupational Health, University of Nebraska Medical Center, Omaha, NE 68198, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Levecke","sequence":"additional","affiliation":[{"name":"Department of Virology, Parasitology and Immunology, Ghent University, 9820 Merelbeke, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohannes","family":"Negash","sequence":"additional","affiliation":[{"name":"Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1537-6639","authenticated-orcid":false,"given":"Abraham","family":"Degarege","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, University of Nebraska Medical Center, Omaha, NE 68198, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,17]]},"reference":[{"key":"ref_1","unstructured":"WHO (2022, January 08). Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/schistosomiasis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/s41572-018-0013-8","article-title":"Schistosomiasis","volume":"4","author":"McManus","year":"2018","journal-title":"Nat. Rev. Dis. Primer"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"60","DOI":"10.3389\/fpubh.2018.00060","article-title":"An Epidemiological Trend of Urogenital Schistosomiasis in Ethiopia","volume":"6","author":"Chala","year":"2018","journal-title":"Front. Public Health"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1128\/CMR.12.1.97","article-title":"Relationship between schistosomiasis and bladder cancer","volume":"12","author":"Mostafa","year":"1999","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"100093","DOI":"10.1016\/j.crpvbd.2022.100093","article-title":"The parasitology of female genital schistosomiasis","volume":"2","author":"Orish","year":"2022","journal-title":"Curr. Res. Parasitol. Vector-Borne Dis."},{"key":"ref_6","unstructured":"CDC (Centers for Disease Control and Prevention) (2018, April 11). Parasites\u2014Schistosomiasis, Available online: https:\/\/www.cdc.gov\/parasites\/schistosomiasis\/index.html."},{"key":"ref_7","unstructured":"Lo, N. (2024, February 28). Available online: https:\/\/iris.who.int\/bitstream\/handle\/10665\/351856\/9789240041608-eng.pdf?sequence=1."},{"key":"ref_8","unstructured":"WHO (World Health Organization) (2023, January 16). Neglected Tropical Diseases. Available online: https:\/\/www.who.int\/news-room\/questions-and-answers\/item\/neglected-tropical-diseases."},{"key":"ref_9","unstructured":"CDC (Centers for Disease Control and Prevention) (2019, August 14). DPDx\u2014Laboratory Identification of Parasites of Public Health Concern: Schistosomiasis, Available online: https:\/\/www.cdc.gov\/dpdx\/schistosomiasis\/index.html."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1007\/s12639-021-01446-2","article-title":"Detecting Schistosoma haematobium infection by microscopy and polymerase chain reaction (PCR) in school children in three senatorial districts of Cross River State, Nigeria","volume":"46","author":"Esiere","year":"2022","journal-title":"J. Parasit. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e238","DOI":"10.3346\/jkms.2018.33.e238","article-title":"Comparison of ELISA and Urine Microscopy for Diagnosis of Schistosoma haematobium Infection","volume":"33","author":"Song","year":"2018","journal-title":"J. Korean Med. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Mohammed, H., Landeryou, T., Chernet, M., Liyew, E.F., Wulataw, Y., Getachew, B., Difabachew, H., Phillips, A., Maddren, R., and Ower, A. (2022). Comparing the accuracy of two diagnostic methods for detection of light Schistosoma haematobium infection in an elimination setting in Wolaita Zone, South Western Ethiopia. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0267378"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1007\/s00436-018-6137-7","article-title":"Improved detection of DNA Schistosoma haematobium from eggs extracted by bead beating in urine","volume":"118","author":"Pomari","year":"2018","journal-title":"Parasitol. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"59","DOI":"10.4269\/ajtmh.18-0988","article-title":"Comparison of Kato-Katz Thick Smear, Mini-FLOTAC, and Flukefinder for the Detection and Quantification of Fasciola hepatica Eggs in Artificially Spiked Human Stool","volume":"101","author":"Vlaminck","year":"2019","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1186\/s13071-023-05890-2","article-title":"Comparison of Mini-FLOTAC, Flukefinder and sedimentation techniques for detection and quantification of Fasciola hepatica and Calicophoron daubneyi eggs using spiked and naturally infected bovine faecal samples","volume":"16","author":"Bosco","year":"2023","journal-title":"Parasit Vectors"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"109435","DOI":"10.1016\/j.vetpar.2021.109435","article-title":"Evaluation of two Fasciola hepatica faecal egg counting protocols in sheep and cattle","volume":"294","author":"Reigate","year":"2021","journal-title":"Vet. Parasitol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/1756-3305-7-107","article-title":"Trematode infections in cattle in Arumeru District, Tanzania are associated with irrigation","volume":"1","author":"Nzalawahe","year":"2014","journal-title":"Parasit Vectors"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Degarege, A., Mekonnen, Z., Levecke, B., Legesse, M., Negash, Y., Vercruysse, J., and Erko, B. (2015). Prevalence of Schistosoma haematobium Infection among School-Age Children in Afar Area, Northeastern Ethiopia. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0133142"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.jbi.2014.02.013","article-title":"Sample size estimation in diagnostic test studies of biomedical informatics","volume":"48","year":"2014","journal-title":"J. Biomed. Inform."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Waligora, M., Dranseika, V., and Piasecki, J. (2014). Child\u2019s assent in research: Age threshold or personalisation?. BMC Medical Ethics, 15.","DOI":"10.1186\/1472-6939-15-44"},{"key":"ref_21","unstructured":"WHO (2024, February 28). Available online: https:\/\/iris.who.int\/handle\/10665\/40793."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Armoo, S., Cunningham, L.J., Campbell, S.J., Aboagye, F.T., Boampong, F.K., Hamidu, B.A., Osei-Atweneboana, M.Y., Stothard, J.R., and Adams, E.R. (2020). Detecting Schistosoma mansoni infections among pre-school-aged children in southern Ghana: A diagnostic comparison of urine-CCA, real-time PCR and Kato-Katz assays. BMC Infect. Dis., 20.","DOI":"10.1186\/s12879-020-05034-2"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Oliveira, W.J., Magalh\u00e3es, F.D.C., Elias, A.M.S., de Castro, V.N., Favero, V., Lindholz, C.G., Oliveira, A., Barbosa, F.S., Gil, F., and Gomes, M.A. (2018). Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test. PLoS Neglected Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006232"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.parint.2011.06.020","article-title":"Comparison of the Kato-Katz and FLOTAC techniques for the diagnosis of soil-transmitted helminth infections","volume":"60","author":"Habtamu","year":"2011","journal-title":"Parasitol. Int."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"107017","DOI":"10.1016\/j.actatropica.2023.107017","article-title":"Evaluation of isotype-based serology for diagnosis of Schistosoma mansoni infection in individuals living in endemic areas with low parasite burden","volume":"248","author":"Moreira","year":"2023","journal-title":"Acta Trop."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2519","DOI":"10.1056\/NEJMoa1812165","article-title":"Schistosomiasis\u2014Assessing Progress toward the 2020 and 2025 Global Goals","volume":"381","author":"Deol","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"276","DOI":"10.11613\/BM.2012.031","article-title":"Interrater reliability: The kappa statistic","volume":"22","author":"McHugh","year":"2012","journal-title":"Biochem. Med."},{"key":"ref_28","unstructured":"Howell, A. (2011). Snail-Borne Diseases in Bovids at High and Low Altitude in Eastern Uganda: Integrated Parasitological and Malacological Mapping, University of Liverpool. Available online: https:\/\/www.researchgate.net\/publication\/267948147_Snail-borne_diseases_in_bovids_at_high_and_low_altitude_in_Eastern_Uganda_Integrated_parasitological_and_malacological_mapping."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"110142","DOI":"10.1016\/j.vetpar.2024.110142","article-title":"In-herd prevalence of Fasciola hepatica and Calicophoron\/Paramphistomum spp. infections in German dairy cows with comparison of two coproscopical methods and establishment of real-time pyrosequencing for rumen fluke species differentiation","volume":"327","author":"Hecker","year":"2024","journal-title":"Veter-Parasitol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"DLing, D., Pai, M., Schiller, I., and Dendukuri, N. (2014). A Bayesian framework for estimating the incremental value of a diagnostic test in the absence of a gold standard. BMC Med. Res. Methodol., 14.","DOI":"10.1186\/1471-2288-14-67"},{"key":"ref_31","unstructured":"Tenny, S., and Hoffman, M. (2024, March 31). Prevalence, StatPearls, Available online: https:\/\/pubmed.ncbi.nlm.nih.gov\/28613617\/."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1186\/s13071-018-3136-6","article-title":"Urogenital schistosomiasis elimination in Zanzibar: Accuracy of urine filtration and haematuria reagent strips for diagnosing light intensity Schistosoma haematobium infections","volume":"11","author":"Knopp","year":"2018","journal-title":"Parasites Vectors"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Knopp, S., Corstjens, P.L.A.M., Koukounari, A., Cercamondi, C.I., Ame, S.M., Ali, S.M., de Dood, C.J., Mohammed, K.A., Utzinger, J., and Rollinson, D. (2015). Sensitivity and Specificity of a Urine Circulating Anodic Antigen Test for the Diagnosis of Schistosoma haematobium in Low Endemic Setting. PLoS Neglected Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003752"}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/14\/10\/1037\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:43:51Z","timestamp":1760107431000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/14\/10\/1037"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,17]]},"references-count":33,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["diagnostics14101037"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics14101037","relation":{},"ISSN":["2075-4418"],"issn-type":[{"type":"electronic","value":"2075-4418"}],"subject":[],"published":{"date-parts":[[2024,5,17]]}}}