{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:36:16Z","timestamp":1788568576072,"version":"build-2803163510"},"reference-count":35,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T00:00:00Z","timestamp":1727222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Federal Ministry of Education and Research","doi-asserted-by":"publisher","award":["13GW0459D"],"award-info":[{"award-number":["13GW0459D"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Rapid molecular assays can be used to identify Candida pathogens directly from positive blood cultures (BCs) in a timely manner compared to standard methods using subcultures. In this study, the eazyplex\u00ae\u00a0Candida ID assay, which is based on loop-mediated amplification (LAMP) and is currently for research use only, was evaluated for the identification of the most common fungal species. A total of 190 BCs were analysed. Sensitivity and specificity were 93.88% and 99.26% for C. albicans, 89.13% and 100% for Nakaseomyces glabratus (N. glabratus), 100% and 100% for Pichia kudravzevii (P. kudriavzevii), 100% and 100% for C. tropicalis, and 100% and 99.44% for C. parapsilosis. Sample preparation took approximately 11 min and positive amplification results were obtained between 8.5 and 19 min. The eazyplex\u00ae\u00a0Candida ID LAMP assay is an easy-to-use diagnostic tool that can optimise the management of patients with candidemia.<\/jats:p>","DOI":"10.3390\/diagnostics14192125","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T16:16:37Z","timestamp":1727280997000},"page":"2125","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of the Eazyplex\u00ae\u00a0Candida ID LAMP Assay for the Rapid Diagnosis of Positive Blood Cultures"],"prefix":"10.3390","volume":"14","author":[{"given":"Arvid","family":"Berlau","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, Jena University Hospital, Friedrich Schiller University, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Stoll","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Jena University Hospital, Friedrich Schiller University, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Edel","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Jena University Hospital, Friedrich Schiller University, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina","family":"L\u00f6ffler","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Jena University Hospital, Friedrich Schiller University, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"R\u00f6del","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Jena University Hospital, Friedrich Schiller University, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1007\/s10096-018-3285-8","article-title":"Analyzing candidemia guideline adherence identifies opportunities for antifungal stewardship","volume":"37","author":"Mellinghoff","year":"2018","journal-title":"Eur. 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