{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T02:07:52Z","timestamp":1784167672309,"version":"3.55.0"},"reference-count":25,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T00:00:00Z","timestamp":1727222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"SD BIOSENSOR"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Interferon-gamma release assays (IGRAs) have gained attention for the diagnosis of latent tuberculosis infection (LTBI) due to their higher specificity compared to the tuberculin skin test (TST). However, the IGRA\u2019s performance varies across different populations. This study evaluated the diagnostic performance of three IGRAs (TBF-FIA, TBF-ELISA, and QFT-Plus) in Ghana, comparing them among individuals exposed and unexposed to MTB infection. Conducted in TB clinics across three regions, this prospective and cross-sectional study included healthy individuals with no known TB exposure (unexposed group) and patients with confirmed active TB (exposed group). Blood samples were tested using all three assays as per the manufacturers\u2019 guidelines. The TBF-ELISA showed 3.4% higher sensitivity but 4.6% lower specificity compared to QFT-Plus. The TBF-FIA had sensitivity of 78.5\u201387.3% and specificity of 82.9\u201390.0%. These findings indicate that while the three IGRAs offer similar diagnostic accuracy, the variations in specificity and limited data on assays like TBF-FIA require further investigation.<\/jats:p>","DOI":"10.3390\/diagnostics14192130","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T16:16:37Z","timestamp":1727280997000},"page":"2130","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of Diagnostic Performance of Three Commercial Interferon-Gamma Release Assays for Mycobacterium tuberculosis"],"prefix":"10.3390","volume":"14","author":[{"given":"Richard","family":"Kutame","sequence":"first","affiliation":[{"name":"National Public Health and Reference Laboratory, Harley Street, Korle Bu Teaching Hospital, Accra P.O. Box GP300, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8833-7235","authenticated-orcid":false,"given":"Gifty","family":"Boateng","sequence":"additional","affiliation":[{"name":"National Public Health and Reference Laboratory, Harley Street, Korle Bu Teaching Hospital, Accra P.O. Box GP300, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaw","family":"Adusi-Poku","sequence":"additional","affiliation":[{"name":"National TB Control Programme, Korle Bu Teaching Hospital, Accra P.O. Box KB493, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Sorvor","sequence":"additional","affiliation":[{"name":"National TB Control Programme, Korle Bu Teaching Hospital, Accra P.O. Box KB493, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorreta","family":"Antwi","sequence":"additional","affiliation":[{"name":"National Public Health and Reference Laboratory, Harley Street, Korle Bu Teaching Hospital, Accra P.O. Box GP300, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9031-8925","authenticated-orcid":false,"given":"Florence","family":"Agyemang-Bioh","sequence":"additional","affiliation":[{"name":"National Public Health and Reference Laboratory, Harley Street, Korle Bu Teaching Hospital, Accra P.O. Box GP300, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bright","family":"Ayensu","sequence":"additional","affiliation":[{"name":"National Public Health and Reference Laboratory, Harley Street, Korle Bu Teaching Hospital, Accra P.O. Box GP300, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Gyau-Boateng","sequence":"additional","affiliation":[{"name":"National Public Health and Reference Laboratory, Harley Street, Korle Bu Teaching Hospital, Accra P.O. Box GP300, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franklin","family":"Asiedu-Bekoe","sequence":"additional","affiliation":[{"name":"Public Health Division, Ghana Health Service, Korle Bu Teaching Hospital, P.M.B. Ministries, Accra P.O. Box MB582, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1016\/S0140-6736(20)30925-9","article-title":"Global burden of 369 diseases and injuries in 204 countries and territories, 1990\u20132019: A systematic analysis for the global burden of disease study 2019","volume":"396","author":"Vos","year":"2020","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2344","DOI":"10.1016\/S0140-6736(15)00323-2","article-title":"Controlling the seedbeds of tuberculosis: Diagnosis and treatment of tuberculosis infection","volume":"386","author":"Rangaka","year":"2015","journal-title":"Lancet"},{"key":"ref_3","unstructured":"(2022). 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