{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:08:10Z","timestamp":1789978090213,"version":"4.0.1"},"reference-count":46,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T00:00:00Z","timestamp":1728864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Vaincre la Mucoviscidose","award":["2010\/IC1020"],"award-info":[{"award-number":["2010\/IC1020"]}]},{"name":"Vaincre la Mucoviscidose","award":["ANR-13-ASTR-0011-01"],"award-info":[{"award-number":["ANR-13-ASTR-0011-01"]}]},{"name":"Agence Nationale de la Recherche","award":["2010\/IC1020"],"award-info":[{"award-number":["2010\/IC1020"]}]},{"name":"Agence Nationale de la Recherche","award":["ANR-13-ASTR-0011-01"],"award-info":[{"award-number":["ANR-13-ASTR-0011-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background\/objectives:\u00a0Pseudomonas aeruginosa can cause community-acquired infections affecting various body sites. The present retrospective study investigated the genetic diversity of 173 isolates (166 clinical, 7 environmental) of P. aeruginosa collected from clinical pathology laboratories in Abidjan, C\u00f4te d\u2019Ivoire (2001\u20132011). Methods: Multiple-Locus Variable Number of Tandem Repeats (VNTR) Analysis (MLVA) using 13 loci was applied to all isolates and compared to published MLVA data. The antibiotics status of the isolates was compiled when available and compared to published profiles. Results: Among 95 isolates analyzed for their antibiotics status, 14 displayed concerning resistance profiles: five multidrug-resistant (MDR) and nine extensively drug-resistant (XDR). MLVA typing revealed a high genetic diversity (&gt;130 genotypes), with many genotypes represented by a single strain. Notably, thirteen clusters (\u22654 related isolates) were observed. Some clusters displayed close genetic relatedness to isolates from France, Korea, and well-studied strains (ST560, LES and PA14). Comparative analysis suggested the presence of international high-risk MDR clones (CC233, CC111) in C\u00f4te d\u2019Ivoire. Importantly, MLVA clustering revealed a close relationship of CC235-MDR strains with a locally identified cluster (group 9). Conclusions: These findings support MLVA as a reliable and cost-effective tool for low-resource settings, allowing the selection of relevant strains for future whole genome sequence analyses. This approach can improve outbreak investigations and public health interventions aimed at curbing MDR P. aeruginosa transmission within hospitals and at the national level.<\/jats:p>","DOI":"10.3390\/diagnostics14202284","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T05:47:58Z","timestamp":1728884878000},"page":"2284","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Typing of Pseudomonas aeruginosa Isolates Collected in Abidjan Hospitals (C\u00f4te d\u2019Ivoire) Using the Multiple-Locus Variable Number of Tandem Repeats Method"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7848-117X","authenticated-orcid":false,"given":"Christiane","family":"Essoh","sequence":"first","affiliation":[{"name":"D\u00e9partement de Biochimie-G\u00e9n\u00e9tique, UFR des Sciences Biologiques, Universit\u00e9 Peleforo Gon Coulibaly (UPGC), Korhogo BP 1328, C\u00f4te d\u2019Ivoire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolande","family":"Hauck","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universit\u00e9 Paris-Saclay, 91198 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5456-3103","authenticated-orcid":false,"given":"Timoth\u00e9e","family":"Ouassa","sequence":"additional","affiliation":[{"name":"Centre de Diagnostic et de Recherches sur le SIDA et les Autres Maladies Infectieuses (CeDReS), CHU de Treichville, Abidjan BPV 03, C\u00f4te d\u2019Ivoire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daouda","family":"Tour\u00e9","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biochimie-G\u00e9n\u00e9tique, UFR des Sciences Biologiques, Universit\u00e9 Peleforo Gon Coulibaly (UPGC), Korhogo BP 1328, C\u00f4te d\u2019Ivoire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richmond","family":"Djatchi","sequence":"additional","affiliation":[{"name":"Centre de Diagnostic et de Recherches sur le SIDA et les Autres Maladies Infectieuses (CeDReS), CHU de Treichville, Abidjan BPV 03, C\u00f4te d\u2019Ivoire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume Yao","family":"Loukou","sequence":"additional","affiliation":[{"name":"Laboratoire National de Sant\u00e9 Publique (LNSP), Abidjan BP 2403, C\u00f4te d\u2019Ivoire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon-Pierre Assanvo","family":"N\u2019Guetta","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique, UFR Biosciences, Universit\u00e9 F\u00e9lix Houphou\u00ebt-Boigny, Abidjan BPV 582, C\u00f4te d\u2019Ivoire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0913-194X","authenticated-orcid":false,"given":"Gilles","family":"Vergnaud","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universit\u00e9 Paris-Saclay, 91198 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Pourcel","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universit\u00e9 Paris-Saclay, 91198 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Moradali, M.F., Ghods, S., and Rehm, B.H. 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