{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:10:44Z","timestamp":1760058644693,"version":"build-2065373602"},"reference-count":42,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T00:00:00Z","timestamp":1744675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background: 16S rRNA analysis has been used in various diseases to identify pathogenic bacteria. In particular, pathogens that are difficult to cultivate or previously unknown can be detected with great certainty. In chronic prostatitis\/chronic pelvic pain syndrome (CP\/CPPS), a distinction between bacterial and non-bacterial genesis is essential with regard to categorization and therapy. The objective of this study is to investigate the value of 16S rRNA gene sequence analysis in the routine management of patients with CP\/CPPS especially after failure to detect a pathogen in conventional culture and polymerase chain reaction for sexually transmitted diseases (STI-PCR). Methods: In total, 228 patients with CP\/CPPS were prospectively enrolled and received a comprehensive andrological work-up. Microbial analysis consisted of standard bacterial cultures and the detection of sexually transmitted pathogens by PCR using urine specimens from a 2-glass test and semen analysis. 16S rRNA gene sequence analysis was performed in patients with urine and semen of patients without bacterial pathogens in microbiological culture and STI-PCR. Results: In 184 of 199 (92%) patients with negative ejaculate culture and negative STI-PCR, no pathogen could be detected by 16S rRNA analysis and in the case of a positive result, the analysis only showed non-pathogenic bacteria of the normal flora. There was no statistical association between the 16S rRNA analysis and the inflammatory markers or the National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI) scores. Conclusions: At least in our study cohort, the 16S rRNA analysis provided no additional benefit following microbiological culture and STI-PCR in the categorization of patients with CP\/CPPS.<\/jats:p>","DOI":"10.3390\/diagnostics15081003","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T03:59:06Z","timestamp":1744689546000},"page":"1003","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["16S rRNA Gene Sequence Analysis of V6\u2013V8 Region Provides Limited Advantage in Diagnosis of Chronic Prostatitis"],"prefix":"10.3390","volume":"15","author":[{"given":"Jens","family":"Rosellen","sequence":"first","affiliation":[{"name":"Department of Urology, Pediatric Urology and Andrology, Justus Liebig University Giessen, 35392 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moritz","family":"Fritzenwanker","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology, Justus Liebig University Giessen, 35390 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0874-0439","authenticated-orcid":false,"given":"Hans-Christian","family":"Schuppe","sequence":"additional","affiliation":[{"name":"Department of Urology, Pediatric Urology and Andrology, Justus Liebig University Giessen, 35392 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0127-4938","authenticated-orcid":false,"given":"Undraga","family":"Schagdarsurengin","sequence":"additional","affiliation":[{"name":"Department of Urology, Pediatric Urology and Andrology, Justus Liebig University Giessen, 35392 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2909-0797","authenticated-orcid":false,"given":"Florian","family":"Wagenlehner","sequence":"additional","affiliation":[{"name":"Department of Urology, Pediatric Urology and Andrology, Justus Liebig University Giessen, 35392 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian","family":"Pilatz","sequence":"additional","affiliation":[{"name":"Department of Urology, Pediatric Urology and Andrology, Justus Liebig University Giessen, 35392 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.juro.2012.08.088","article-title":"The prevalence and overlap of interstitial cystitis\/bladder pain syndrome and chronic prostatitis\/chronic pelvic pain syndrome in men: Results of the RAND Interstitial Cystitis Epidemiology male study","volume":"189","author":"Suskind","year":"2013","journal-title":"J. Urol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S85","DOI":"10.1016\/j.ijantimicag.2007.08.028","article-title":"Epidemiology of prostatitis","volume":"31","author":"Krieger","year":"2008","journal-title":"Int. J. Antimicrob. Agents."},{"key":"ref_3","first-page":"397","article-title":"Prostatitis: Diagnosis and treatment","volume":"82","author":"Sharp","year":"2010","journal-title":"Am. Fam. Physician."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1097\/01.ju.0000082252.49374.e9","article-title":"Chronic Prostatitis Collaborative Research Network Study Group. Leukocytes and bacteria in men with chronic prostatitis\/chronic pelvic pain syndrome compared to asymptomatic controls","volume":"170","author":"Nickel","year":"2003","journal-title":"J. Urol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0090-4295(02)01733-8","article-title":"Cytologic evaluation of urine is important in evaluation of chronic prostatitis","volume":"60","author":"Nickel","year":"2002","journal-title":"Urology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1111\/bju.13101","article-title":"Prostatitis Expert Reference Group (PERG). Diagnosis and treatment of chronic bacterial prostatitis and chronic prostatitis\/chronic pelvic pain syndrome: A consensus guideline","volume":"116","author":"Rees","year":"2015","journal-title":"BJU Int."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1001\/jama.282.3.236","article-title":"NIH consensus definition and classification of prostatitis","volume":"282","author":"Krieger","year":"1999","journal-title":"JAMA"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S0022-5347(06)00498-8","article-title":"How does the pre-massage and post-massage 2-glass test compare to the Meares-Stamey 4-glass test in men with chronic prostatitis\/chronic pelvic pain syndrome?","volume":"176","author":"Nickel","year":"2006","journal-title":"J. Urol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1016\/j.fertnstert.2012.01.124","article-title":"The incidence and effect of bacteriospermia and elevated seminal leukocytes on semen parameters","volume":"97","author":"Domes","year":"2012","journal-title":"Fertil Steril."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Baud, D., Pattaroni, C., Vulliemoz, N., Castella, V., Marsland, B.J., and Stojanov, M. (2019). Sperm microbiota and its impact on semen parameters. Front Microbiol., 10.","DOI":"10.3389\/fmicb.2019.00234"},{"key":"ref_11","first-page":"596","article-title":"Assessment of bacterial diversity in fresh bubaline semen","volume":"82","author":"Rana","year":"2012","journal-title":"Indian J. Anim. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1093\/humupd\/dmy048","article-title":"Identification and evaluation of the microbiome in the female and male reproductive tracts","volume":"25","author":"Koedooder","year":"2019","journal-title":"Hum. Reprod. Update"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e00053-19","DOI":"10.1128\/CMR.00053-19","article-title":"Performance and Application of 16S rRNA Gene Cycle Sequencing for Routine Identification of Bacteria in the Clinical Microbiology Laboratory","volume":"33","author":"Church","year":"2020","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.mimet.2006.05.009","article-title":"16S rRNA sequencing in routine bacterial identification: A 30-month experiment","volume":"67","author":"Mignard","year":"2006","journal-title":"J. Microbiol. Methods"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/S1369-5274(99)80052-6","article-title":"Ribosomal DNA sequencing as a tool for identification of bacterial pathogens","volume":"2","author":"Kolbert","year":"1999","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1128\/CMR.17.4.840-862.2004","article-title":"Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases","volume":"17","author":"Clarridge","year":"2004","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Angiuoli, S.V., White, J.R., Matalka, M., White, O., and Fricke, W.F. (2011). Resources and costs for microbial sequence analysis evaluated using virtual machines and cloud computing. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0026624"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e00450-20","DOI":"10.1128\/msphere.00450-20","article-title":"Enterobacterales Infection after Intestinal Dominance in Hospitalized Patients","volume":"5","author":"Rao","year":"2020","journal-title":"mSphere"},{"key":"ref_19","first-page":"557","article-title":"Assessment of phylogenetic affiliation using 16S rRNA gene sequence analysis for Pseudomonas aeruginosa in patients of lower respiratory tract infection","volume":"138","author":"Tripathi","year":"2013","journal-title":"Indian J. Med. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/j.jmii.2019.01.002","article-title":"The microbiological characteristics of lower respiratory tract infection in patients with neuromuscular disorders: An investigation based on a multiplex polymerase chain reaction to detect viruses and a clone library analysis of the bacterial 16S rRNA gene sequence in sputum samples","volume":"52","author":"Ogawa","year":"2019","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Marshall, C.W., Kurs-Lasky, M., McElheny, C.L., Bridwell, S., Liu, H., and Shaikh, N. (2021). Performance of Conventional Urine Culture Compared to 16S rRNA Gene Amplicon Sequencing in Children with Suspected Urinary Tract Infection. Microbiol. Spectr., 9.","DOI":"10.1128\/spectrum.01861-21"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e01452-18","DOI":"10.1128\/JCM.01452-18","article-title":"Reassessment of Routine Midstream Culture in Diagnosis of Urinary Tract Infection","volume":"57","author":"Sathiananthamoorthy","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"42","DOI":"10.5582\/ddt.2020.01000","article-title":"Usefulness of next-generation DNA sequencing for the diagnosis of urinary tract infection","volume":"14","author":"Ishihara","year":"2020","journal-title":"Drug Discov. Ther."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Kawai, Y., Ozawa, N., Fukuda, T., Suzuki, N., and Mikata, K. (2022). Development of an efficient antimicrobial susceptibility testing method with species identification by Nanopore sequencing of 16S rRNA amplicons. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0262912"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Song, W.J., Gao, J., Huang, J.W., Liu, Y., Long, Z., and He, L.Y. (2023). Is type III prostatitis also associated with bacterial infection?. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1189081"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Rosellen, J., Dittmar, F., Hauptmann, A., Diemer, T., Schuppe, H.C., Schagdarsurengin, U., Fritzenwanker, M., Wagenlehner, F., and Pilatz, A. (2024). Impaired Semen Quality in Patients with Chronic Prostatitis. J. Clin. Med., 13.","DOI":"10.3390\/jcm13102884"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Berg, E., Houska, P., Nesheim, N., Schuppe, H.C., Pilatz, A., Fijak, M., Manthey, M., Steger, K., Wagenlehner, F., and Schagdarsurengin, U. (2021). Chronic Prostatitis\/Chronic Pelvic Pain Syndrome Leads to Impaired Semen Parameters, Increased Sperm DNA Fragmentation and Unfavorable Changes of Sperm Protamine mRNA Ratio. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22157854"},{"key":"ref_28","first-page":"349","article-title":"Reference values for testicular volume, epididymal head size and peak systolic velocity of the testicular artery in adult males measured by ultrasonography","volume":"34","author":"Pilatz","year":"2013","journal-title":"Ultraschall Med."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Aprikian, S., Luz, M., Brimo, F., Scarlata, E., Hamel, L., Cury, F.L., Tanguay, S., Aprikian, A.G., Kassouf, W., and Chevalier, S. (2019). Improving ultrasound-based prostate volume estimation. BMC Urol., 19.","DOI":"10.1186\/s12894-019-0492-2"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5500","DOI":"10.1128\/JCM.41.12.5500-5510.2003","article-title":"Culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients","volume":"41","author":"Domann","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_31","unstructured":"World Health Organization (2010). WHO Laboratory Manual for the Examination and Processing of Human Semen, WHO. [5th ed.]."},{"key":"ref_32","unstructured":"World Health Organization (2021). WHO Laboratory Manual for the Examination and Processing of Human Semen, WHO. [6th ed.]."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"188","DOI":"10.4103\/iju.IJU_407_16","article-title":"Semen culture and the assessment of genitourinary tract infections","volume":"33","author":"Solomon","year":"2017","journal-title":"Indian J. Urol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1007\/s001200050428","article-title":"Das Prostatitissyndrom. Ejakulatver\u00e4nderungen und Auswirkungen auf die Fertilit\u00e4t [Prostatitis syndrome. Changes in the ejaculate and effects on fertility]","volume":"40","author":"Ludwig","year":"2001","journal-title":"Urol. A"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Ipe, D.S., Horton, E., and Ulett, G.C. (2016). The Basics of Bacteriuria: Strategies of Microbes for Persistence in Urine. Front. Cell. Infect. Microbiol., 6.","DOI":"10.3389\/fcimb.2016.00014"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1007\/s12098-023-04627-4","article-title":"16S Ribosomal RNA Analysis for the Diagnosis of Urinary Tract Infection of Infants: Has the Time Arrived?","volume":"90","author":"Goel","year":"2023","journal-title":"Indian J. Pediatr."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.euf.2016.11.001","article-title":"The Urinary Tract Microbiome in Health and Disease","volume":"4","author":"Castillo","year":"2018","journal-title":"Eur. Urol. Focus."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1111\/and.12457","article-title":"Seminal Corynebacterium strains in infertile men with and without leucocytospermia","volume":"48","author":"Mashaly","year":"2016","journal-title":"Andrologia"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2614692","DOI":"10.1155\/2016\/2614692","article-title":"Bacteriospermia and its impact on basic semen parameters among infertile men","volume":"2016","author":"Vilvanathan","year":"2016","journal-title":"Interdiscip. Perspect. Infect. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/s10815-008-9283-5","article-title":"The presence of bacteria species in semen and sperm quality","volume":"26","author":"Moretti","year":"2009","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/S0015-0282(16)47609-0","article-title":"The effect of Ureaplasma urealyticum on semen characteristics","volume":"41","author":"Upadhyaya","year":"1984","journal-title":"Fertil Steril."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/S0022-5347(17)55498-1","article-title":"Ureaplasma urealyticum (T-mycoplasma) infection: Does it have a role in male infertility?","volume":"124","author":"Desai","year":"1980","journal-title":"J. Urol."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/15\/8\/1003\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:14:37Z","timestamp":1760030077000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/15\/8\/1003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,15]]},"references-count":42,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["diagnostics15081003"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics15081003","relation":{},"ISSN":["2075-4418"],"issn-type":[{"type":"electronic","value":"2075-4418"}],"subject":[],"published":{"date-parts":[[2025,4,15]]}}}