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A timely and accurate diagnosis of IBD is essential because diagnostic delays can result in intestinal damage that is irreversible, leading in some cases to intestinal dysfunction and the need for surgery. Diagnostic delays are common in cases when GI symptoms are mild and nonspecific. When IBD is suspected, the common diagnostic algorithm includes laboratory analyses, cross-sectional radiologic imaging, and endoscopy with biopsy and histological analysis. Other diagnostic biomarkers, including those found in the serum, stool, and urine, have also been evaluated in IBD. Newer artificial intelligence (AI)-based technologies are now being developed, and these will likely play an important future role in the diagnosis and management of IBD.<\/jats:p>","DOI":"10.3390\/diagnostics15111303","type":"journal-article","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T04:32:37Z","timestamp":1747888357000},"page":"1303","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Diagnostic Methods and Biomarkers in Inflammatory Bowel Disease"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4366-351X","authenticated-orcid":false,"given":"Andrew M.","family":"Kaz","sequence":"first","affiliation":[{"name":"GI Section, Hospital and Specialty Medicine, VA Puget Sound Health Care System, Seattle, WA 98108, USA"},{"name":"Department of Medicine\/Gastroenterology, University of Washington School of Medicine, Seattle, WA 98195, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanda","family":"Venu","sequence":"additional","affiliation":[{"name":"Department of Medicine\/Gastroenterology, University of Washington School of Medicine, Seattle, WA 98195, USA"},{"name":"Center for Digestive Health, Virginia Mason Franciscan Health, Seattle, WA 98101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1756","DOI":"10.1016\/S0140-6736(16)32126-2","article-title":"Ulcerative colitis","volume":"389","author":"Ungaro","year":"2017","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1016\/S0140-6736(16)31711-1","article-title":"Crohn\u2019s disease","volume":"389","author":"Torres","year":"2017","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Diez-Martin, E., Hernandez-Suarez, L., Munoz-Villafranca, C., Martin-Souto, L., Astigarraga, E., Ramirez-Garcia, A., and Barreda-Gomez, G. (2024). Inflammatory Bowel Disease: A Comprehensive Analysis of Molecular Bases, Predictive Biomarkers, Diagnostic Methods, and Therapeutic Options. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25137062"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Zhou, J.L., Bao, J.C., Liao, X.Y., Chen, Y.J., Wang, L.W., Fan, Y.Y., Xu, Q.Y., Hao, L.X., Li, K.J., and Liang, M.X. (2023). Trends and projections of inflammatory bowel disease at the global, regional and national levels, 1990-2050: A bayesian age-period-cohort modeling study. BMC Public Health, 23.","DOI":"10.1186\/s12889-023-17431-8"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e065186","DOI":"10.1136\/bmjopen-2022-065186","article-title":"Global, regional and national burden of inflammatory bowel disease in 204 countries and territories from 1990 to 2019: A systematic analysis based on the Global Burden of Disease Study 2019","volume":"13","author":"Wang","year":"2023","journal-title":"BMJ Open"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1053\/j.gastro.2019.01.002","article-title":"Past and Future Burden of Inflammatory Bowel Diseases Based on Modeling of Population-Based Data","volume":"156","author":"Coward","year":"2019","journal-title":"Gastroenterology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1002\/ueg2.12317","article-title":"Implications of the changing epidemiology of inflammatory bowel disease in a changing world","volume":"10","author":"Agrawal","year":"2022","journal-title":"United Eur. Gastroenterol. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"105676","DOI":"10.1016\/j.envint.2020.105676","article-title":"Ambient air pollution and the risk of pediatric-onset inflammatory bowel disease: A population-based cohort study","volume":"138","author":"Elten","year":"2020","journal-title":"Environ. Int."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1038\/s41564-021-00970-4","article-title":"Bifidobacterium species associated with breastfeeding produce aromatic lactic acids in the infant gut","volume":"6","author":"Laursen","year":"2021","journal-title":"Nat. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"n1554","DOI":"10.1136\/bmj.n1554","article-title":"Association of ultra-processed food intake with risk of inflammatory bowel disease: Prospective cohort study","volume":"374","author":"Narula","year":"2021","journal-title":"BMJ"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1053\/j.gastro.2021.04.063","article-title":"Approach to the Management of Recently Diagnosed Inflammatory Bowel Disease Patients: A User\u2019s Guide for Adult and Pediatric Gastroenterologists","volume":"161","author":"Agrawal","year":"2021","journal-title":"Gastroenterology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1111\/jebm.12626","article-title":"Diagnosis and management of inflammatory bowel disease","volume":"17","author":"Li","year":"2024","journal-title":"J. Evid. Based Med."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Scheurlen, K.M., Parks, M.A., Macleod, A., and Galandiuk, S. (2023). Unmet Challenges in Patients with Crohn\u2019s Disease. J. Clin. Med., 12.","DOI":"10.3390\/jcm12175595"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Sorrentino, D., Nguyen, V.Q., and Chitnavis, M.V. (2019). Capturing the Biologic Onset of Inflammatory Bowel Diseases: Impact on Translational and Clinical Science. Cells, 8.","DOI":"10.3390\/cells8060548"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1038\/ajg.2010.343","article-title":"Frequency and risk factors for extraintestinal manifestations in the Swiss inflammatory bowel disease cohort","volume":"106","author":"Vavricka","year":"2011","journal-title":"Am. J. Gastroenterol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1097\/MIB.0000000000000429","article-title":"Chronological Order of Appearance of Extraintestinal Manifestations Relative to the Time of IBD Diagnosis in the Swiss Inflammatory Bowel Disease Cohort","volume":"21","author":"Vavricka","year":"2015","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1053\/j.gastro.2021.07.042","article-title":"Extraintestinal Manifestations of Inflammatory Bowel Disease: Current Concepts, Treatment, and Implications for Disease Management","volume":"161","author":"Rogler","year":"2021","journal-title":"Gastroenterology"},{"key":"ref_18","first-page":"661","article-title":"Crohn\u2019s Disease: Diagnosis and Management","volume":"98","author":"Veauthier","year":"2018","journal-title":"Am. Fam. Physician"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.14309\/ajg.0000000000002326","article-title":"Management of Perianal Crohn\u2019s Disease","volume":"118","author":"Parian","year":"2023","journal-title":"Am. J. Gastroenterol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2521","DOI":"10.3748\/wjg.v27.i20.2521","article-title":"Evolving role of endoscopy in inflammatory bowel disease: Going beyond diagnosis","volume":"27","author":"Nunez","year":"2021","journal-title":"World J. Gastroenterol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1002\/ibd.20398","article-title":"Endoscopic evaluation of patients with inflammatory bowel disease","volume":"14","author":"Simpson","year":"2008","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1038\/ajg.2009.727","article-title":"Ulcerative colitis practice guidelines in adults: American College of Gastroenterology, Practice Parameters Committee","volume":"105","author":"Kornbluth","year":"2010","journal-title":"Am. J. Gastroenterol."},{"key":"ref_23","first-page":"1275","article-title":"Patchy cecal inflammation associated with distal ulcerative colitis: A prospective endoscopic study","volume":"92","author":"Geboes","year":"1997","journal-title":"Am. J. Gastroenterol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1016\/j.crohns.2013.09.016","article-title":"European evidence based consensus for endoscopy in inflammatory bowel disease","volume":"7","author":"Annese","year":"2013","journal-title":"J. Crohns Colitis"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/j.crohns.2013.06.001","article-title":"European consensus on the histopathology of inflammatory bowel disease","volume":"7","author":"Magro","year":"2013","journal-title":"J. Crohns Colitis"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1046\/j.1365-2559.2002.01416.x","article-title":"Granulomatous ulcerative colitis: A re-appraisal of the mucosal granuloma in the distinction of Crohn\u2019s disease from ulcerative colitis","volume":"41","author":"Mahadeva","year":"2002","journal-title":"Histopathology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1136\/jclinpath-2013-201885","article-title":"Inflammatory bowel disease biopsies: Updated British Society of Gastroenterology reporting guidelines","volume":"66","author":"Feakins","year":"2013","journal-title":"J. Clin. Pathol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2587","DOI":"10.1136\/gutjnl-2021-326562","article-title":"Recent advances in clinical practice: Advances in cross-sectional imaging in inflammatory bowel disease","volume":"71","author":"Rimola","year":"2022","journal-title":"Gut"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1856","DOI":"10.1002\/med.21893","article-title":"Inflammatory bowel disease biomarkers","volume":"42","author":"Liu","year":"2022","journal-title":"Med. Res. Rev."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1016\/j.suc.2019.08.001","article-title":"Inflammatory Bowel Disease Presentation and Diagnosis","volume":"99","author":"Flynn","year":"2019","journal-title":"Surg. Clin. N. Am."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1148\/radiol.2471070611","article-title":"Inflammatory bowel disease diagnosed with US, MR, scintigraphy, and CT: Meta-analysis of prospective studies","volume":"247","author":"Horsthuis","year":"2008","journal-title":"Radiology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1016\/j.crohns.2013.02.020","article-title":"Imaging techniques for assessment of inflammatory bowel disease: Joint ECCO and ESGAR evidence-based consensus guidelines","volume":"7","author":"Panes","year":"2013","journal-title":"J. Crohns Colitis"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1016\/j.cgh.2012.03.026","article-title":"Endoscopic skipping of the distal terminal ileum in Crohn\u2019s disease can lead to negative results from ileocolonoscopy","volume":"10","author":"Samuel","year":"2012","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1053\/j.gastro.2013.10.055","article-title":"Accuracy of magnetic resonance enterography in assessing response to therapy and mucosal healing in patients with Crohn\u2019s disease","volume":"146","author":"Ordas","year":"2014","journal-title":"Gastroenterology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/j.1365-2036.2011.04710.x","article-title":"Systematic review: The use of ultrasonography, computed tomography and magnetic resonance imaging for the diagnosis, assessment of activity and abdominal complications of Crohn\u2019s disease","volume":"34","author":"Panes","year":"2011","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s11894-024-00915-x","article-title":"Clinical Application of Intestinal Ultrasound in Inflammatory Bowel Disease","volume":"26","author":"Kellar","year":"2024","journal-title":"Curr. Gastroenterol. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1002\/jgh3.12268","article-title":"Gastrointestinal ultrasound in inflammatory bowel disease care: Patient perceptions and impact on disease-related knowledge","volume":"4","author":"Rajagopalan","year":"2020","journal-title":"JGH Open"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1097\/MPG.0000000000003618","article-title":"Pediatric Patient and Caregiver Satisfaction with the Use of Transabdominal Bowel Ultrasound in the Assessment of Inflammatory Bowel Diseases","volume":"76","author":"Hudson","year":"2023","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1097\/MIB.0000000000001174","article-title":"A Simple Ultrasound Score for the Accurate Detection of Inflammatory Activity in Crohn\u2019s Disease","volume":"23","author":"Novak","year":"2017","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1111\/apt.15767","article-title":"Transperineal ultrasound predicts endoscopic and histological healing in ulcerative colitis","volume":"51","author":"Sagami","year":"2020","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1093\/ibd\/izy091","article-title":"Real-time Interobserver Agreement in Bowel Ultrasonography for Diagnostic Assessment in Patients with Crohn\u2019s Disease: An International Multicenter Study","volume":"24","author":"Calabrese","year":"2018","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1097\/MIB.0b013e3182802b87","article-title":"Noninvasive diagnosis of small bowel Crohn\u2019s disease: Direct comparison of bowel sonography and magnetic resonance enterography","volume":"19","author":"Castiglione","year":"2013","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1007\/s00330-013-2973-0","article-title":"Ultrasound as a diagnostic tool in detecting active Crohn\u2019s disease: A meta-analysis of prospective studies","volume":"24","author":"Dong","year":"2014","journal-title":"Eur. Radiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1093\/ecco-jcc\/jjy113","article-title":"ECCO-ESGAR Guideline for Diagnostic Assessment in IBD Part 1: Initial diagnosis, monitoring of known IBD, detection of complications","volume":"13","author":"Maaser","year":"2019","journal-title":"J. Crohns Colitis"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1097\/00054725-200407000-00008","article-title":"Clinical relevance of transabdominal ultrasonography and magnetic resonance imaging in patients with inflammatory bowel disease of the terminal ileum and large bowel","volume":"10","author":"Pascu","year":"2004","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.giec.2024.07.002","article-title":"Capsule Endoscopy in Inflammatory Bowel Disease: Evolving Role and Recent Advances","volume":"35","author":"Eidler","year":"2025","journal-title":"Gastrointest. Endosc. Clin. N. Am."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1053\/j.gastro.2011.01.055","article-title":"Epidemiology and natural history of inflammatory bowel diseases","volume":"140","author":"Cosnes","year":"2011","journal-title":"Gastroenterology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.giec.2020.12.004","article-title":"Role of Capsule Endoscopy in Inflammatory Bowel Disease","volume":"31","author":"McCain","year":"2021","journal-title":"Gastrointest. Endosc. Clin. N. Am."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1038\/ajg.2018.27","article-title":"ACG Clinical Guideline: Management of Crohn\u2019s Disease in Adults","volume":"113","author":"Lichtenstein","year":"2018","journal-title":"Am. J. Gastroenterol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1055\/a-1973-3796","article-title":"Small-bowel capsule endoscopy and device-assisted enteroscopy for diagnosis and treatment of small-bowel disorders: European Society of Gastrointestinal Endoscopy (ESGE) Guideline\u2014Update 2022","volume":"55","author":"Pennazio","year":"2023","journal-title":"Endoscopy"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1038\/ajg.2009.713","article-title":"Capsule endoscopy has a significantly higher diagnostic yield in patients with suspected and established small-bowel Crohn\u2019s disease: A meta-analysis","volume":"105","author":"Dionisio","year":"2010","journal-title":"Am. J. Gastroenterol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1016\/j.dld.2017.04.013","article-title":"Diagnostic yield of capsule endoscopy versus magnetic resonance enterography and small bowel contrast ultrasound in the evaluation of small bowel Crohn\u2019s disease: Systematic review and meta-analysis","volume":"49","author":"Kopylov","year":"2017","journal-title":"Dig. Liver Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.gie.2008.02.017","article-title":"Small-bowel imaging in Crohn\u2019s disease: A prospective, blinded, 4-way comparison trial","volume":"68","author":"Solem","year":"2008","journal-title":"Gastrointest. Endosc."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1097\/MEG.0000000000001083","article-title":"Development and validation of a scoring index to predict the presence of lesions in capsule endoscopy in patients with suspected Crohn\u2019s disease of the small bowel: A Spanish multicenter study","volume":"30","year":"2018","journal-title":"Eur. J. Gastroenterol. Hepatol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1097\/MOG.0000000000000519","article-title":"The role of small bowel endoscopy for Crohn\u2019s disease","volume":"35","author":"Ellul","year":"2019","journal-title":"Curr. Opin. Gastroenterol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1177\/2050640613508551","article-title":"Capsule endoscopy: High negative predictive value in the long term despite a low diagnostic yield in patients with suspected Crohn\u2019s disease","volume":"1","author":"Hall","year":"2013","journal-title":"United Eur. Gastroenterol. J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"128","DOI":"10.5946\/ce.2015.48.2.128","article-title":"Guidelines for video capsule endoscopy: Emphasis on Crohn\u2019s disease","volume":"48","author":"Park","year":"2015","journal-title":"Clin. Endosc."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1097\/01.mcg.0000225633.14663.64","article-title":"Video capsule endoscopy in patients with known or suspected small bowel stricture previously tested with the dissolving patency capsule","volume":"41","author":"Spada","year":"2007","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.crohns.2013.09.004","article-title":"Advanced endoscopic imaging techniques in Crohn\u2019s disease","volume":"8","author":"Tontini","year":"2014","journal-title":"J. Crohns Colitis"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"384","DOI":"10.14309\/ajg.0000000000000152","article-title":"ACG Clinical Guideline: Ulcerative Colitis in Adults","volume":"114","author":"Rubin","year":"2019","journal-title":"Am. J. Gastroenterol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1007\/s10620-013-2907-3","article-title":"Correlations of C-reactive protein levels and erythrocyte sedimentation rates with endoscopic activity indices in patients with ulcerative colitis","volume":"59","author":"Yoon","year":"2014","journal-title":"Dig. Dis. Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.3109\/00365521.2015.1064991","article-title":"Risk matrix model for prediction of colectomy in a population-based study of ulcerative colitis patients (the IBSEN study)","volume":"50","author":"Solberg","year":"2015","journal-title":"Scand. J. Gastroenterol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1136\/gut.2005.069476","article-title":"Laboratory markers in IBD: Useful, magic, or unnecessary toys?","volume":"55","author":"Vermeire","year":"2006","journal-title":"Gut"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.crohns.2011.05.003","article-title":"C-reactive protein (CRP), erythrocyte sedimentation rate (ESR) or both? A systematic evaluation in pediatric ulcerative colitis","volume":"5","author":"Turner","year":"2011","journal-title":"J. Crohns Colitis"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1097\/MIB.0b013e318280b19e","article-title":"Combined serological, genetic, and inflammatory markers differentiate non-IBD, Crohn\u2019s disease, and ulcerative colitis patients","volume":"19","author":"Plevy","year":"2013","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_66","first-page":"93","article-title":"Diagnostic Value of ASCA and Atypical p-ANCA in Differential Diagnosis of Inflammatory Bowel Disease","volume":"5","author":"Mokhtarifar","year":"2013","journal-title":"Middle East. J. Dig. Dis."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2235","DOI":"10.1111\/j.1572-0241.2004.40369.x","article-title":"Serologic testing with ANCA, ASCA, and anti-OmpC in children and young adults with Crohn\u2019s disease and ulcerative colitis: Diagnostic value and correlation with disease phenotype","volume":"99","author":"Zholudev","year":"2004","journal-title":"Am. J. Gastroenterol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1053\/j.gastro.2005.03.046","article-title":"Antibodies to CBir1 flagellin define a unique response that is associated independently with complicated Crohn\u2019s disease","volume":"128","author":"Targan","year":"2005","journal-title":"Gastroenterology"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2410","DOI":"10.1111\/j.1572-0241.2006.00840.x","article-title":"Diagnostic precision of anti-Saccharomyces cerevisiae antibodies and perinuclear antineutrophil cytoplasmic antibodies in inflammatory bowel disease","volume":"101","author":"Reese","year":"2006","journal-title":"Am. J. Gastroenterol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/S2468-1253(16)30111-X","article-title":"Genetics of inflammatory bowel disease: Beyond NOD2","volume":"2","author":"Mirkov","year":"2017","journal-title":"Lancet Gastroenterol. Hepatol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"522","DOI":"10.3892\/mmr.2014.2180","article-title":"Faecal calprotectin as a novel biomarker for differentiating between inflammatory bowel disease and irritable bowel syndrome","volume":"10","author":"Chang","year":"2014","journal-title":"Mol. Med. Rep."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1111\/j.1572-0241.2007.01126.x","article-title":"Diagnostic precision of fecal calprotectin for inflammatory bowel disease and colorectal malignancy","volume":"102","author":"Karamountzos","year":"2007","journal-title":"Am. J. Gastroenterol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1097\/01.MIB.0000187980.08686.18","article-title":"Comparison of 4 neutrophil-derived proteins in feces as indicators of disease activity in ulcerative colitis","volume":"11","author":"Langhorst","year":"2005","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"5","DOI":"10.17305\/bjbms.2013.2402","article-title":"Nitric oxide as a potential biomarker in inflammatory bowel disease","volume":"13","author":"Avdagic","year":"2013","journal-title":"Bosn. J. Basic. Med. Sci."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1093\/clinchem\/47.7.1297","article-title":"Tumor necrosis factor-alpha in serum of patients with inflammatory bowel disease as measured by a highly sensitive immuno-PCR","volume":"47","author":"Komatsu","year":"2001","journal-title":"Clin. Chem."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1136\/jim-2016-000062","article-title":"Serum ST2 in inflammatory bowel disease: A potential biomarker for disease activity","volume":"64","author":"Boga","year":"2016","journal-title":"J. Investig. Med."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"473","DOI":"10.2217\/bmm.16.9","article-title":"TNFAIP6 is a potential biomarker of disease activity in inflammatory bowel disease","volume":"10","author":"Yu","year":"2016","journal-title":"Biomark. Med."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"17562848241251600","DOI":"10.1177\/17562848241251600","article-title":"Biomarkers in inflammatory bowel disease: A practical guide","volume":"17","author":"Clough","year":"2024","journal-title":"Ther. Adv. Gastroenterol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1038\/nm.4307","article-title":"Oncostatin M drives intestinal inflammation and predicts response to tumor necrosis factor-neutralizing therapy in patients with inflammatory bowel disease","volume":"23","author":"West","year":"2017","journal-title":"Nat. Med."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.1093\/ibd\/izab032","article-title":"Oncostatin M Is a Biomarker of Diagnosis, Worse Disease Prognosis, and Therapeutic Nonresponse in Inflammatory Bowel Disease","volume":"27","author":"Verstockt","year":"2021","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"2383","DOI":"10.1053\/j.gastro.2021.02.019","article-title":"Identification of an Anti-Integrin alphavbeta6 Autoantibody in Patients with Ulcerative Colitis","volume":"160","author":"Kuwada","year":"2021","journal-title":"Gastroenterology"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1097\/01.ibd.0000227626.72271.91","article-title":"Fecal S100A12: A novel noninvasive marker in children with Crohn\u2019s disease","volume":"12","author":"Leach","year":"2006","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1111\/jgh.13598","article-title":"Fecal neutrophil gelatinase-associated lipocalin as a biomarker for inflammatory bowel disease","volume":"32","author":"Thorsvik","year":"2017","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1111\/apt.18154","article-title":"Faecal biomarkers for diagnosis and prediction of disease course in treatment-naive patients with IBD","volume":"60","author":"Peterson","year":"2024","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1038\/nri1132","article-title":"The immunological and genetic basis of inflammatory bowel disease","volume":"3","author":"Bouma","year":"2003","journal-title":"Nat. Rev. Immunol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1002\/ibd.20419","article-title":"Increased incidence of urinary matrix metalloproteinases as predictors of disease in pediatric patients with inflammatory bowel disease","volume":"14","author":"Manfredi","year":"2008","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Hagiwara, S.I., Abe, N., Hosoi, K., Hara, T., Ishige, T., Shimizu, H., Mizuochi, T., Kakiuchi, T., Kunisaki, R., and Matsuoka, R. (2023). Utility of a rapid assay for prostaglandin E-major urinary metabolite as a biomarker in pediatric ulcerative colitis. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-37145-6"},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Oliveira, E.C.S., Quaglio, A.E.V., Magro, D.O., Di Stasi, L.C., and Sassaki, L.Y. (2023). Intestinal Microbiota and miRNA in IBD: A Narrative Review about Discoveries and Perspectives for the Future. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24087176"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"e2703","DOI":"10.1097\/MD.0000000000002703","article-title":"Circulating MicroRNA223 is a New Biomarker for Inflammatory Bowel Disease","volume":"95","author":"Wang","year":"2016","journal-title":"Medicine"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"e00134","DOI":"10.14309\/ctg.0000000000000134","article-title":"MicroRNA-320a Monitors Intestinal Disease Activity in Patients with Inflammatory Bowel Disease","volume":"11","author":"Cordes","year":"2020","journal-title":"Clin. Transl. Gastroenterol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"3000605221089503","DOI":"10.1177\/03000605221089503","article-title":"MicroRNAs as potential biomarkers for the diagnosis of inflammatory bowel disease: A systematic review and meta-analysis","volume":"50","author":"Sun","year":"2022","journal-title":"J. Int. Med. Res."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Heydari, R., Karimi, P., and Meyfour, A. (2024). Long non-coding RNAs as pathophysiological regulators, therapeutic targets and novel extracellular vesicle biomarkers for the diagnosis of inflammatory bowel disease. Biomed. Pharmacother., 176.","DOI":"10.1016\/j.biopha.2024.116868"},{"key":"ref_93","first-page":"2195","article-title":"KIF9-AS1, LINC01272 and DIO3OS lncRNAs as novel biomarkers for inflammatory bowel disease","volume":"17","author":"Wang","year":"2018","journal-title":"Mol. Med. Rep."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"109472","DOI":"10.1016\/j.intimp.2022.109472","article-title":"Metabolomics window into the diagnosis and treatment of inflammatory bowel disease in recent 5 years","volume":"113","author":"Wang","year":"2022","journal-title":"Int. Immunopharmacol."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1136\/gutjnl-2016-312135","article-title":"Interplay of host genetics and gut microbiota underlying the onset and clinical presentation of inflammatory bowel disease","volume":"67","author":"Imhann","year":"2018","journal-title":"Gut"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/s41564-018-0306-4","article-title":"Gut microbiome structure and metabolic activity in inflammatory bowel disease","volume":"4","author":"Franzosa","year":"2019","journal-title":"Nat. Microbiol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"461503","DOI":"10.1016\/j.chroma.2020.461503","article-title":"Integrated study of metabolomics and gut metabolic activity from ulcerative colitis to colorectal cancer: The combined action of disordered gut microbiota and linoleic acid metabolic pathway might fuel cancer","volume":"1629","author":"Tang","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"e2200003","DOI":"10.1002\/prca.202200003","article-title":"Proteomic and lipidomic biomarkers in the diagnosis and progression of inflammatory bowel disease\u2014A review","volume":"17","author":"Ronkowski","year":"2023","journal-title":"Proteom. Clin. Appl."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.jprot.2018.10.017","article-title":"Integrated serum proteins and fatty acids analysis for putative biomarker discovery in inflammatory bowel disease","volume":"195","author":"Manfredi","year":"2019","journal-title":"J. Proteom."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1002\/ueg2.12338","article-title":"The role of gut microbiome in inflammatory bowel disease diagnosis and prognosis","volume":"10","author":"Zheng","year":"2022","journal-title":"United Eur. Gastroenterol. J."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1136\/gutjnl-2016-313235","article-title":"A microbial signature for Crohn\u2019s disease","volume":"66","author":"Pascal","year":"2017","journal-title":"Gut"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"6481","DOI":"10.1016\/j.csbj.2021.11.037","article-title":"Dysbiosis and relapse-related microbiome in inflammatory bowel disease: A shotgun metagenomic approach","volume":"19","author":"Mayorga","year":"2021","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"3555","DOI":"10.1038\/s41591-024-03280-4","article-title":"Noninvasive, microbiome-based diagnosis of inflammatory bowel disease","volume":"30","author":"Zheng","year":"2024","journal-title":"Nat. Med."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1093\/ecco-jcc\/jjv005","article-title":"The diagnostic value of a new fecal marker, matrix metalloprotease-9, in different types of inflammatory bowel diseases","volume":"9","author":"Farkas","year":"2015","journal-title":"J. Crohns Colitis"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1007\/s10620-012-2027-5","article-title":"Evaluation of fecal myeloperoxidase as a biomarker of disease activity and severity in ulcerative colitis","volume":"57","author":"Masoodi","year":"2012","journal-title":"Dig. Dis. Sci."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/ibd\/izy046","article-title":"Circulating and Fecal microRNAs as Biomarkers for Inflammatory Bowel Diseases","volume":"24","author":"Schonauen","year":"2018","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"e027043","DOI":"10.1136\/bmjopen-2018-027043","article-title":"Cost-effectiveness of faecal calprotectin used in primary care in the diagnosis of inflammatory bowel disease","volume":"9","author":"Zhang","year":"2019","journal-title":"BMJ Open"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1016\/j.crohns.2014.08.005","article-title":"Accuracy and cost of diagnostic strategies for patients with suspected Crohn\u2019s disease","volume":"8","author":"Maconi","year":"2014","journal-title":"J. Crohns Colitis"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3310\/hta23660","article-title":"Magnetic resonance enterography compared with ultrasonography in newly diagnosed and relapsing Crohn\u2019s disease patients: The METRIC diagnostic accuracy study","volume":"23","author":"Taylor","year":"2019","journal-title":"Health Technol. Assess."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1093\/ibd\/izac115","article-title":"A Systematic Review of Artificial Intelligence and Machine Learning Applications to Inflammatory Bowel Disease, with Practical Guidelines for Interpretation","volume":"28","author":"Stafford","year":"2022","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"17562848211017730","DOI":"10.1177\/17562848211017730","article-title":"Artificial intelligence in gastrointestinal endoscopy for inflammatory bowel disease: A systematic review and new horizons","volume":"14","author":"Tontini","year":"2021","journal-title":"Ther. Adv. Gastroenterol."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"e193963","DOI":"10.1001\/jamanetworkopen.2019.3963","article-title":"Performance of a Deep Learning Model vs Human Reviewers in Grading Endoscopic Disease Severity of Patients with Ulcerative Colitis","volume":"2","author":"Stidham","year":"2019","journal-title":"JAMA Netw. Open"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"2150","DOI":"10.1053\/j.gastro.2020.02.012","article-title":"Development and Validation of a Deep Neural Network for Accurate Evaluation of Endoscopic Images from Patients with Ulcerative Colitis","volume":"158","author":"Takenaka","year":"2020","journal-title":"Gastroenterology"},{"key":"ref_114","first-page":"418","article-title":"Endoscopy and central reading in inflammatory bowel disease clinical trials: Achievements, challenges and future developments","volume":"70","author":"Gottlieb","year":"2021","journal-title":"Gut"},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1093\/ecco-jcc\/jjac152","article-title":"Application of Deep Learning Models to Improve Ulcerative Colitis Endoscopic Disease Activity Scoring Under Multiple Scoring Systems","volume":"17","author":"Byrne","year":"2023","journal-title":"J. Crohns Colitis"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1053\/j.gastro.2020.09.053","article-title":"Computer-Aided Diagnosis with Monochromatic Light Endoscopy for Scoring Histologic Remission in Ulcerative Colitis","volume":"160","author":"Bossuyt","year":"2021","journal-title":"Gastroenterology"},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"E273","DOI":"10.1055\/a-1261-2944","article-title":"Can artificial intelligence help to detect dysplasia in patients with ulcerative colitis?","volume":"53","author":"Maeda","year":"2021","journal-title":"Endoscopy"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1111\/den.13517","article-title":"Clinical usefulness of a deep learning-based system as the first screening on small-bowel capsule endoscopy reading","volume":"32","author":"Aoki","year":"2020","journal-title":"Dig. Endosc."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"516","DOI":"10.5009\/gnl220507","article-title":"Capsule Endoscopy in Inflammatory Bowel Disease: Panenteric Capsule Endoscopy and Application of Artificial Intelligence","volume":"17","author":"Ukashi","year":"2023","journal-title":"Gut Liver"},{"key":"ref_120","doi-asserted-by":"crossref","unstructured":"Holland, R., Patel, U., Lung, P., Chotzoglou, E., and Kainz, B. (2019). Automatic Detection of Bowel Disease with Residual Networks, Springer International Publishing.","DOI":"10.1007\/978-3-030-32281-6_16"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1007\/978-3-031-18814-5_3","article-title":"Cross-scale Attention Guided Multi-instance Learning for Crohn\u2019s Disease Diagnosis with Pathological Images","volume":"13594","author":"Deng","year":"2022","journal-title":"Multiscale Multimodal Med. Imaging"},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1136\/gutjnl-2019-320065","article-title":"Big data in IBD: Big progress for clinical practice","volume":"69","author":"Madgwick","year":"2020","journal-title":"Gut"},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"G328","DOI":"10.1152\/ajpgi.00360.2020","article-title":"Gut microbiome-based supervised machine learning for clinical diagnosis of inflammatory bowel diseases","volume":"320","author":"Manandhar","year":"2021","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_124","first-page":"165","article-title":"Machine Learning Based Metagenomic Prediction of Inflammatory Bowel Disease","volume":"285","author":"Mihajlovic","year":"2021","journal-title":"Stud. Health Technol. Inform."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1002\/ueg2.12655","article-title":"Inflammatory bowel disease genomics, transcriptomics, proteomics and metagenomics meet artificial intelligence","volume":"12","author":"Cannarozzi","year":"2024","journal-title":"United Eur. Gastroenterol. J."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.cgh.2024.05.048","article-title":"How Artificial Intelligence Will Transform Clinical Care, Research, and Trials for Inflammatory Bowel Disease","volume":"23","author":"Silverman","year":"2025","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"283","DOI":"10.5217\/ir.2023.00020","article-title":"Artificial intelligence in inflammatory bowel disease: Implications for clinical practice and future directions","volume":"21","author":"Ahmad","year":"2023","journal-title":"Intest. Res."},{"key":"ref_128","doi-asserted-by":"crossref","unstructured":"Majidova, K., Handfield, J., Kafi, K., Martin, R.D., and Kubinski, R. (2021). Role of Digital Health and Artificial Intelligence in Inflammatory Bowel Disease: A Scoping Review. Genes, 12.","DOI":"10.3390\/genes12101465"},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1126\/science.aax2342","article-title":"Dissecting racial bias in an algorithm used to manage the health of populations","volume":"366","author":"Obermeyer","year":"2019","journal-title":"Science"},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1007\/s12553-017-0179-1","article-title":"Google DeepMind and healthcare in an age of algorithms","volume":"7","author":"Powles","year":"2017","journal-title":"Health Technol."},{"key":"ref_131","doi-asserted-by":"crossref","unstructured":"Crew, A., Reidy, C., van der Westhuizen, H.M., and Graham, M. (2024). A Narrative Review of Ethical Issues in the Use of Artificial Intelligence Enabled Diagnostics for Diabetic Retinopathy. J. Eval. Clin. Pract., 1\u201312.","DOI":"10.1111\/jep.14237"},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1111\/jgh.15521","article-title":"Mapping ethico-legal principles for the use of artificial intelligence in gastroenterology","volume":"36","author":"Stewart","year":"2021","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1136\/gutjnl-2021-326271","article-title":"Artificial intelligence in gastroenterology and hepatology: How to advance clinical practice while ensuring health equity","volume":"71","author":"Berzin","year":"2022","journal-title":"Gut"},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1007\/s10620-024-08557-9","article-title":"Ethical Implications of Artificial Intelligence in Gastroenterology: The Co-pilot or the Captain?","volume":"69","author":"Aggarwal","year":"2024","journal-title":"Dig. Dis. Sci."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"3954","DOI":"10.3748\/wjg.v30.i35.3954","article-title":"Delayed diagnosis in inflammatory bowel disease: Time to consider solutions","volume":"30","author":"Lv","year":"2024","journal-title":"World J. Gastroenterol."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/15\/11\/1303\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:38:05Z","timestamp":1760031485000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/15\/11\/1303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,22]]},"references-count":135,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["diagnostics15111303"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics15111303","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,22]]}}}