{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T09:53:35Z","timestamp":1784886815349,"version":"3.55.0"},"reference-count":54,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n, Spain","doi-asserted-by":"publisher","award":["PID2020-113919RB-I00\/AEI\/10.13039\/501100011033"],"award-info":[{"award-number":["PID2020-113919RB-I00\/AEI\/10.13039\/501100011033"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background\/Objectives: The transparency and biomechanical properties of the human cornea are governed by the precise organization of collagen fibers. A novel quantitative technique to analyze corneal collagen organization, based on intensity gradient modeling and probability density function (PDF) fitting, is proposed. Methods: Derived from second-harmonic generation (SHG) images, the method calculates image gradients, derives PDFs of gradient orientations, and fits them to Gaussian models. Results: Tested across species and temporal healing stages, this approach is an advantageous alternative to traditional methods like Fourier transform and structure tensor analyses, particularly in noisy or low-contrast conditions. Conclusions: The technique offers a scalable, robust framework suitable for research, clinical diagnostics, and treatment monitoring.<\/jats:p>","DOI":"10.3390\/diagnostics15141738","type":"journal-article","created":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T11:58:07Z","timestamp":1751975887000},"page":"1738","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Quantitative Characterization of Corneal Collagen Architecture Using Intensity Gradient Modeling and Gaussian PDF Fitting"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6334-691X","authenticated-orcid":false,"given":"Enrique J.","family":"Fernandez","sequence":"first","affiliation":[{"name":"Laboratorio de \u00d3ptica, Instituto Universitario de Investigaci\u00f3n en \u00d3ptica y Nanof\u00edsica, Campus de Espinardo (Ed. 34), CEIR Campus Mare Nostrum (CMN), Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9711-8034","authenticated-orcid":false,"given":"Juan M.","family":"Bueno","sequence":"additional","affiliation":[{"name":"Laboratorio de \u00d3ptica, Instituto Universitario de Investigaci\u00f3n en \u00d3ptica y Nanof\u00edsica, Campus de Espinardo (Ed. 34), CEIR Campus Mare Nostrum (CMN), Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.preteyeres.2015.07.001","article-title":"Corneal structure and transparency","volume":"49","author":"Meek","year":"2015","journal-title":"Prog. 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