{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:40:39Z","timestamp":1760060439098,"version":"build-2065373602"},"reference-count":22,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T00:00:00Z","timestamp":1755820800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PON Ricerca e Innovazione 2014\u20132020","award":["DM1062\/2021","411 PE00000007, INF-ACT"],"award-info":[{"award-number":["DM1062\/2021","411 PE00000007, INF-ACT"]}]},{"name":"MUR PNRR Extended Partnership Initiative on Emerging Infectious Diseases","award":["DM1062\/2021","411 PE00000007, INF-ACT"],"award-info":[{"award-number":["DM1062\/2021","411 PE00000007, INF-ACT"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background\/Objectives. Multi-drug-resistant (MDR) microorganisms pose a significant challenge in healthcare settings, particularly with beta-lactam-resistant Gram-negative bacteria and glycopeptide-resistant enterococci. Culture represents the most reliable technique in determining their presence within surveillance swabs. However, it requires a long time-to-result (TTR) and shows low sensitivity. Molecular techniques integrate diagnostic procedures, allowing TTR reduction and precise identification of genes. Methods. During our usual surveillance campaign, we had the opportunity to evaluate the Allplex Entero-DR assay (Seegene Inc., Seoul, Republic of Korea) and the Entero-DR Plus assay (Arrow Diagnostics srl, Genova, Italy) molecular kits for the detection of extended-\u03b2-lactamases (ESBL), carbapenem- and vancomycin-resistant genes, as well as Acinetobacter spp. and Pseudomonas aeruginosa spp. identification directly from rectal swabs. A comparison between these tests and the culture-based routine completed the study. Results. The analysis included 300 rectal swabs from the University Hospital Policlinico (Catania, Italy). One hundred and eighty-eight samples (62.6%) resulted as positive for at least one Allplex\u2122 target, reaching optimal sensitivity and negative predictive value (100%). Our results underlined the ubiquitous blaCTX-M and van genes presence and demonstrated the diffusion of double-carbapenemases genes and metallo-\u03b2-lactamases-producing strains. In our epidemiological setting, few data were collected about carbapenem-resistant P. aeruginosa and Acinetobacter spp., which require further evaluations on simultaneous respiratory colonization and higher sample numbers. Conclusions. Our analysis highlighted the importance of combining conventional and advanced diagnostic methods in investigating MDR pathogens. The right approach should be based on the prevalence and variability of resistance mechanisms within a specific epidemiological area. Remarkably, molecular screenings may exclude negative samples within high-risk areas due to a significant negative predictive value.<\/jats:p>","DOI":"10.3390\/diagnostics15172128","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T15:57:47Z","timestamp":1755878267000},"page":"2128","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Active Microbiological Surveillance for Contrasting Multi-Drug-Resistant Pathogens: Comparison Between a Multiplex Real-Time PCR Method and Culture"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4457-0737","authenticated-orcid":false,"given":"Gaetano","family":"Maugeri","sequence":"first","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences (BIOMETEC), University of Catania, 95123 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0256-956X","authenticated-orcid":false,"given":"Maddalena","family":"Calvo","sequence":"additional","affiliation":[{"name":"U.O.C. Laboratory Analysis Unit, A.O.U. \u201cPoliclinico-San Marco\u201d, 95123 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido","family":"Scalia","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences (BIOMETEC), University of Catania, 95123 Catania, Italy"},{"name":"U.O.C. Laboratory Analysis Unit, A.O.U. \u201cPoliclinico-San Marco\u201d, 95123 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1594-7427","authenticated-orcid":false,"given":"Stefania","family":"Stefani","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Biotechnological Sciences (BIOMETEC), University of Catania, 95123 Catania, Italy"},{"name":"U.O.C. Laboratory Analysis Unit, A.O.U. \u201cPoliclinico-San Marco\u201d, 95123 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,22]]},"reference":[{"doi-asserted-by":"crossref","unstructured":"Mojica, M.F., De La Cadena, E., Correa, A., Appel, T.M., Pallares, C.J., and Villegas, M.V. (2020). Evaluation of Allplex\u2122 Entero-DR assay for detection of antimicrobial resistance determinants from bacterial cultures. BMC Res. Notes, 13.","key":"ref_1","DOI":"10.1186\/s13104-020-04997-4"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.jhin.2021.03.027","article-title":"Molecular detection of carbapenem resistance genes in rectal swabs from patients in Gulf Cooperation Council hospitals","volume":"112","author":"Alqahtani","year":"2021","journal-title":"J. Hosp. Infect."},{"unstructured":"(2025, May 25). 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