{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:40:12Z","timestamp":1760060412336,"version":"build-2065373602"},"reference-count":12,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T00:00:00Z","timestamp":1756166400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health","award":["R01CA195443"],"award-info":[{"award-number":["R01CA195443"]}]},{"name":"Department of Radiation Oncology at Stanford University","award":["R01CA195443"],"award-info":[{"award-number":["R01CA195443"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>We report a low-cost protocol and platform for whole-abdomen 3D dynamic contrast-enhanced ultrasound (DCE-US) imaging in mice using a clinical matrix-array transducer. Background\/Objectives: This platform addresses common limitations of preclinical ultrasound systems. In particular, these systems often lack real-time volumetric and molecular imaging capabilities. Methods: Using a modified silicone cup and water bath configuration, mice with dual subcutaneous tumors were imaged in vivo on a clinical EPIQ 7 system equipped with an X6-1 transducer. Results: Intravenous administration of targeted microbubbles enabled high-resolution, contrast-mode 3D imaging at multiple time points. Volumetric reconstructions captured both tumors and surrounding anatomy in a single scan, while time\u2013intensity curves and Differential Targeted Enhancement (DTE) analysis revealed greater microbubble uptake in irradiated tumors, consistent with elevated P-selectin expression. Conclusions: This standardized imaging platform enables whole-abdomen molecular DCE-US in preclinical studies, facilitating intra-animal comparisons of vascular and molecular features across lesions or organs.<\/jats:p>","DOI":"10.3390\/diagnostics15172149","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T06:25:57Z","timestamp":1756189557000},"page":"2149","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preclinical Water-Mediated Ultrasound Platform Using Clinical Field of View for Molecular Targeted Contrast-Enhanced Ultrasound"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3941-8784","authenticated-orcid":false,"given":"Stavros","family":"Melemenidis","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Stephanie","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA"},{"name":"Lyons VA Medical Center, 151 Knollcroft Rd, Lyons, NJ 07939, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenny M.","family":"Vo-Phamhi","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA"},{"name":"Columbia University Vagelos College of Physicians & Surgeons, 630 W 168th Street, New York, NY 10032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4265-9041","authenticated-orcid":false,"given":"Edward E.","family":"Graves","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed Nagy","family":"El Kaffas","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA"},{"name":"Department of Radiology, University of California, San Diego, La Jolla, CA 92037, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitre","family":"Hristov","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.kint.2020.02.011","article-title":"Ultrasound super-resolution imaging provides a noninvasive assessment of renal microvasculature changes during mouse acute kidney injury","volume":"98","author":"Chen","year":"2020","journal-title":"Kidney Int."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/rda.13587","article-title":"Advanced ultrasound techniques in small animal reproduction imaging","volume":"55","author":"Mantziaras","year":"2020","journal-title":"Reprod. 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