{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T05:34:26Z","timestamp":1763789666825,"version":"3.45.0"},"reference-count":26,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministry of Health, Italy","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]},{"name":"European Union\u2014Next Generation EU"},{"name":"IDRA project \u201cDevelopment of Multiparametric Approach for New Instant Digital Pathology to Support Fast Diagnostic Precision and Appropriate Clinical Decision\u201d","award":["PNRR-POC-2022-12376531"],"award-info":[{"award-number":["PNRR-POC-2022-12376531"]}]},{"name":"PNRR-M6C2.2 Investment \u201cEnhancing and strengthening biomedical research in the National Health Service"},{"name":"CUP MASTER","award":["F83C22002490001"],"award-info":[{"award-number":["F83C22002490001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background and Clinical Significance: The paper investigates the use of the Histolog\u00ae Scanner, a confocal microscopy\u2013based device, as a potential tool for intraoperative neuropathological diagnosis of brain tumors. Traditional intraoperative diagnosis, relying on frozen sections and squash preparations, can introduce artifacts and consume valuable tissue. The Histolog\u00ae Scanner offers a plug-and-play solution capable of acquiring high-resolution images of fresh tissue surfaces in minutes while preserving tissue for further histological or molecular analyses. Cases Presentation: Three clinical cases\u2014two women and one-man, mean age 57.3 years\u2014undergoing neurosurgery for distinct brain lesions were included. Tissue samples were immersed in fluorescent dye, rinsed, and immediately analyzed with the Histolog\u00ae Scanner before standard intraoperative histopathology. In the first case, a glioblastoma wild-type, traditional methods struggled to define tumor margins, whereas the device provided rapid, detailed imaging to guide resection. In the second case, a meningioma, the scanner confirmed lesion identity quickly, eliminating the need for a cryostat and reducing artifacts. In the third case, a brain metastasis, integration with cytological apposition allowed simultaneous assessment of lesion margins and nature without freezing the tissue. Conclusions: The Histolog\u00ae Scanner demonstrated multiple advantages: rapid intraoperative use, clear margin visualization, preservation of tissue for subsequent analyses, reduce unnecessary resection, thereby helping to lower the risk of recurrence. This device may complement standard intraoperative methods, enhancing diagnostic accuracy and influencing postoperative treatment planning. Overall, the Histolog\u00ae Scanner represents an innovative tool combining speed, precision, and tissue preservation, suggesting a promising role in establishing a new standard for intraoperative neurosurgical diagnosis.<\/jats:p>","DOI":"10.3390\/diagnostics15222936","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T08:59:46Z","timestamp":1763715586000},"page":"2936","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Laser Confocal Microscopy May Be a Useful Tool in Neuropathological Intraoperative Examination"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9992-2287","authenticated-orcid":false,"given":"Deborah","family":"Dardano","sequence":"first","affiliation":[{"name":"Department of Health Sciences, University \u201cMagna Gr\u00e6cia\u201d of Catanzaro, 88100 Catanzaro, Italy"},{"name":"\u201cR. Dulbecco\u201d University Hospital, 88100 Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Bilotta","sequence":"additional","affiliation":[{"name":"\u201cR. Dulbecco\u201d University Hospital, 88100 Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3099-1305","authenticated-orcid":false,"given":"Gianmarco","family":"Gallucci","sequence":"additional","affiliation":[{"name":"\u201cR. Dulbecco\u201d University Hospital, 88100 Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1379-0458","authenticated-orcid":false,"given":"Carlo","family":"Gentile","sequence":"additional","affiliation":[{"name":"Anatomical Pathology Unit, University Hospital \u201cR. Dulbecco\u201d, 88100 Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Riganati","sequence":"additional","affiliation":[{"name":"Anatomical Pathology Unit, University Hospital \u201cR. Dulbecco\u201d, 88100 Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Veraldi","sequence":"additional","affiliation":[{"name":"Anatomical Pathology Unit, University Hospital \u201cR. Dulbecco\u201d, 88100 Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Domenico","family":"Policicchio","sequence":"additional","affiliation":[{"name":"\u201cR. Dulbecco\u201d University Hospital, 88100 Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Teresa","family":"Nevolo","sequence":"additional","affiliation":[{"name":"Anatomical Pathology Unit, University Hospital \u201cR. Dulbecco\u201d, 88100 Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto V.","family":"Filardo","sequence":"additional","affiliation":[{"name":"Anatomical Pathology Unit, University Hospital \u201cR. Dulbecco\u201d, 88100 Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Maria","family":"Lavecchia","sequence":"additional","affiliation":[{"name":"Anatomical Pathology Unit, University Hospital \u201cR. Dulbecco\u201d, 88100 Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3065-5492","authenticated-orcid":false,"given":"Giuseppe","family":"Donato","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, University \u201cMagna Gr\u00e6cia\u201d of Catanzaro, 88100 Catanzaro, Italy"},{"name":"\u201cR. Dulbecco\u201d University Hospital, 88100 Catanzaro, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1159\/000495175","article-title":"Accuracy of Intraoperative Examination in Central Nervous System Lesions: A Study of 133 Cases","volume":"63","author":"Balsimelli","year":"2019","journal-title":"Acta Cytol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Picart, T., Gautheron, A., Caredda, C., Ray, C., Mahieu-Williame, L., Montcel, B., and Guyotat, J. (2024). Fluorescence-Guided Surgical Techniques in Adult Diffuse Low-Grade Gliomas: State-of-the-Art and Emerging Techniques: A Systematic Review. 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