{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:07:16Z","timestamp":1790878036457,"version":"4.1.0"},"reference-count":36,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32300080"],"award-info":[{"award-number":["32300080"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background: Rapid pathogen detection is crucial for the timely containment of outbreaks, particularly for respiratory infectious diseases which are highly transmissible and possess high epidemic potential. Methods: We developed a sensitive reverse transcription recombinase-aided amplification (RT-RAA) assay for the rapid detection of six common respiratory viruses: respiratory syncytial virus type A (RSV A), influenza A virus (Flu A), influenza B virus (Flu B), human parainfluenza virus (HPIV), SARS-CoV-2 and adenovirus (ADV). The assay employs a single, standardized protocol for the on-demand detection of any one of the six targets. Its performance was validated using nucleic acid standards and clinical pharyngeal swab specimens. Results: The assay enables rapid detection within 20 min at 39 \u00b0C using a portable, self-powered device. It demonstrated high sensitivity, with detection limits below 103 copies\/mL for all targets and as low as 101 copies\/mL for ADV. Cross-reactivity testing with 21 other pathogens confirmed excellent specificity. Validation with 85 clinical samples showed 100% concordance with RT-PCR, while offering significantly faster results and enhanced portability compared to RT-PCR. Conclusions: This sensitive, specific, and user-friendly RT-RAA assay provides a robust tool for rapid detection of respiratory viruses, particularly suitable for deployment in resource-limited settings and point-of-care testing during outbreaks.<\/jats:p>","DOI":"10.3390\/diagnostics16010009","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T12:05:35Z","timestamp":1766145935000},"page":"9","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Rapid and Sensitive Fluorescent RT-RAA Assay for the Detection of a Panel of Six Respiratory Viruses"],"prefix":"10.3390","volume":"16","author":[{"given":"Xudong","family":"Guo","sequence":"first","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongli","family":"Gao","sequence":"additional","affiliation":[{"name":"Jiangyin Center for Disease Control and Prevention, Jiangyin 214400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0193-2510","authenticated-orcid":false,"given":"Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanying","family":"Liu","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbo","family":"Liu","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9845-2405","authenticated-orcid":false,"given":"Rongtao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbin","family":"Song","sequence":"additional","affiliation":[{"name":"Chinese PLA Center for Disease Control and Prevention, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1002\/jmv.25674","article-title":"Recent advances in the detection of respiratory virus infection in humans","volume":"92","author":"Zhang","year":"2020","journal-title":"J. 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