{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T09:04:34Z","timestamp":1766999074898,"version":"3.48.0"},"reference-count":26,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:00:00Z","timestamp":1766707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"bioM\u00e9rieux, Republic of Korea"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background: Accurate identification of pneumonia pathogens is critical for guiding appropriate antibiotic therapy and minimizing unnecessary antimicrobial exposure. Bronchoalveolar lavage (BAL) is widely used for pathogen detection but introduces procedural risks. Bronchial washing (BW) is simpler and less invasive, yet evidence for its utility in multiplex PCR diagnostics is limited. Methods: This study includes an evaluation of the clinical utility of the BioFire\u00ae FilmArray\u00ae Pneumonia Panel (FA) using BW specimens via comparison with conventional culture. Between 2022 and 2024, 190 BW specimens were collected from 182 adult patients with suspected pneumonia at Eunpyeong St. Mary\u2019s Hospital, Seoul, Korea. Each specimen was tested simultaneously using FA and conventional culture. Results: The culture positivity rate was 41.6%, whereas FA showed a higher positivity rate of 51.1%. Of all specimens, 52.6% (100\/190) were positive in at least one of two methods, and 11.0% (21\/190) were FA-positive only. FA detected 72 additional bacterial targets, most commonly H. influenzae, K. pneumoniae, S. aureus, S. agalactiae, and S. pneumoniae. Semi-quantitative results demonstrated a statistically significant moderate correlation with culture (\u03c1 = 0.48, p &lt; 0.001). Eight bacterial targets achieved 100% PPA, and resistance genes were rapidly detected, although some discrepancies with phenotypic antimicrobial susceptibility testing were observed. Several FA-only detections may reflect oropharyngeal colonization rather than true infection. Conclusions: FA testing of BW specimens demonstrated high concordance with culture and provided rapid pathogen and resistance gene detection. BW-based FA testing may serve as a useful diagnostic alternative when BAL is not feasible, although careful interpretation is required to account for potential contamination.<\/jats:p>","DOI":"10.3390\/diagnostics16010091","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T08:47:27Z","timestamp":1766998047000},"page":"91","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Utility of the BioFire\u00ae FilmArray\u00ae Pneumonia Panel Using Bronchial Washing Specimens: A Comparative Study with Conventional Culture"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5216-7732","authenticated-orcid":false,"given":"Sungjin","family":"Jo","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Eunpyeong St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 03312, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sei Won","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Department of Internal Medicine, Eunpyeong St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 03312, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4136-1537","authenticated-orcid":false,"given":"Jung Ok","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Eunpyeong St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 03312, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang-hyun","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Eunpyeong St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 03312, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sehee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Eunpyeong St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 03312, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heayon","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Department of Internal Medicine, Eunpyeong St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 03312, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4103-7921","authenticated-orcid":false,"given":"Chang Dong","family":"Yeo","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Department of Internal Medicine, Eunpyeong St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 03312, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Haak","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Department of Internal Medicine, Eunpyeong St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 03312, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1505-846X","authenticated-orcid":false,"given":"In Young","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2182-5821","authenticated-orcid":false,"given":"Yeon-Joon","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e61","DOI":"10.1093\/cid\/ciw353","article-title":"Management of Adults With Hospital-acquired and Ventilator-associated Pneumonia: 2016 Clinical Practice Guidelines by the Infectious Diseases Society of America and the American Thoracic Society","volume":"63","author":"Kalil","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1111\/j.1469-0691.2007.01915.x","article-title":"Factors impacting on length of stay and mortality of community-acquired pneumonia","volume":"14","author":"Garau","year":"2008","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"S27","DOI":"10.1086\/511159","article-title":"Infectious Diseases Society of America\/American Thoracic Society consensus guidelines on the management of community-acquired pneumonia in adults","volume":"44","author":"Mandell","year":"2007","journal-title":"Clin. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.1183\/13993003.01144-2016","article-title":"Laboratory diagnosis of pneumonia in the molecular age","volume":"48","author":"Torres","year":"2016","journal-title":"Eur. Respir. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e00135-20","DOI":"10.1128\/JCM.00135-20","article-title":"Practical Comparison of the BioFire FilmArray Pneumonia Panel to Routine Diagnostic Methods and Potential Impact on Antimicrobial Stewardship in Adult Hospitalized Patients with Lower Respiratory Tract Infections","volume":"58","author":"Buchan","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"i1","DOI":"10.1136\/thoraxjnl-2013-203618","article-title":"British Thoracic Society guideline for diagnostic flexible bronchoscopy in adults: Accredited by NICE","volume":"68","author":"Blaikley","year":"2013","journal-title":"Thorax"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"334","DOI":"10.5492\/wjccm.v10.i6.334","article-title":"Role of bronchoscopy in critically ill patients managed in intermediate care units\u2014Indications and complications: A narrative review","volume":"10","author":"Menditto","year":"2021","journal-title":"World J. Crit. Care Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1007\/s15010-024-02238-5","article-title":"The agreement between bronchoalveolar lavage, bronchial wash and sputum culture: A retrospective study","volume":"52","author":"Post","year":"2024","journal-title":"Infection"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e00973-18","DOI":"10.1128\/JCM.00973-18","article-title":"Diagnostic Performance of Multiplex Nucleic Acid Testing of Bronchoalveolar Lavage and Bronchial Wash Specimens for Respiratory Viral Pathogens","volume":"56","author":"Munigala","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"39736","DOI":"10.18632\/oncotarget.14504","article-title":"Diagnosis of cytomegalovirus pneumonia by quantitative polymerase chain reaction using bronchial washing fluid from patients with hematologic malignancies","volume":"8","author":"Lee","year":"2017","journal-title":"Oncotarget"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1093\/cid\/ciy584","article-title":"A Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases: 2018 Update by the Infectious Diseases Society of America and the American Society for Microbiology","volume":"67","author":"Miller","year":"2018","journal-title":"Clin. Infect. Dis."},{"key":"ref_12","unstructured":"American Society for Microbiology (2023). Clinical Microbiology Precedures Handbook, ASM Press. [5th ed.]."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1378\/chest.100.1.179","article-title":"The role of bronchoalveolar lavage in diagnosing nonopportunistic bacterial pneumonia","volume":"100","author":"Meduri","year":"1991","journal-title":"Chest"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Schnabel, R.M., van der Velden, K., Osinski, A., Rohde, G., Roekaerts, P.M.H.J., and Bergmans, D.C.J.J. (2015). Clinical course and complications following diagnostic bronchoalveolar lavage in critically ill mechanically ventilated patients. BMC Pulm. Med., 15.","DOI":"10.1186\/s12890-015-0104-1"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"236","DOI":"10.4103\/ijmy.ijmy_77_22","article-title":"The diagnostic yield and characteristics of bronchoalveolar lavage in suspected nontuberculous mycobacterial pulmonary disease","volume":"11","author":"Kajikawa","year":"2022","journal-title":"Int. J. Mycobacteriol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e00128-20","DOI":"10.1128\/JCM.00128-20","article-title":"Multicenter Evaluation of the BioFire FilmArray Pneumonia\/Pneumonia Plus Panel for Detection and Quantification of Agents of Lower Respiratory Tract Infection","volume":"58","author":"Murphy","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0732-8893(02)00372-3","article-title":"Culturing bronchial washings obtained during bronchoscopy fails to add diagnostic utility to culturing the bronchoalveolar lavage fluid alone","volume":"43","author":"Pinckard","year":"2002","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1128\/CMR.7.4.533","article-title":"Bronchoscopic diagnosis of pneumonia","volume":"7","author":"Baselski","year":"1994","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1080\/23744235.2020.1755053","article-title":"Evaluation of the Biofire Filmarray Pneumonia panel plus for lower respiratory tract infections","volume":"52","author":"Edin","year":"2020","journal-title":"Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e00037","DOI":"10.1128\/mBio.00037-15","article-title":"Analysis of the upper respiratory tract microbiotas as the source of the lung and gastric microbiotas in healthy individuals","volume":"6","author":"Bassis","year":"2015","journal-title":"mBio"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Dickson, R.P., and Huffnagle, G.B. (2015). The Lung Microbiome: New Principles for Respiratory Bacteriology in Health and Disease. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1004923"},{"key":"ref_22","unstructured":"BioFire Diagnostics, LLC (2022). FilmArray\u00ae Pneumonia Panel Instructions for Use, BioFire Diagnostics, LLC."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1056\/NEJMoa1500245","article-title":"Community-Acquired Pneumonia Requiring Hospitalization among U.S. Adults","volume":"373","author":"Jain","year":"2015","journal-title":"N. Engl. J. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/j.1469-0691.2009.03092.x","article-title":"Methods for screening for methicillin-resistant Staphylococcus aureus carriage","volume":"15","author":"French","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1128\/JCM.00326-08","article-title":"Current trends in rapid diagnostics for methicillin-resistant Staphylococcus aureus and glycopeptide-resistant enterococcus species","volume":"46","author":"Haccuria","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Polisena, J., Chen, S., Cimon, K., McGill, S., Forward, K., and Gardam, M. (2011). Clinical effectiveness of rapid tests for methicillin resistant Staphylococcus aureus (MRSA) in hospitalized patients: A systematic review. BMC Infect. 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