{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T02:35:25Z","timestamp":1770086125659,"version":"3.49.0"},"reference-count":37,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002657","name":"Scientific Research Projects Coordination Unit of Istanbul University","doi-asserted-by":"publisher","award":["TSA-2022-39123"],"award-info":[{"award-number":["TSA-2022-39123"]}],"id":[{"id":"10.13039\/501100002657","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background\/Objectives: Cell-free DNA (cfDNA) is a valuable biomarker for cancer diagnosis and therapy monitoring; however, its low abundance and fragmented nature present major challenges for reliable isolation, particularly from limited plasma volumes. Here, we report the development and evaluation of a novel magnetically assisted microfluidic chip with a three-inlet design for efficient cfDNA extraction from small-volume plasma samples. Methods: The platform enables controlled infusion of plasma, lysis buffer, and magnetic nanoparticle suspensions at defined flow rates. An external magnetic field selectively captures cfDNA-bound nanoparticles while efficiently removing background impurities. Results: Direct comparison with two in vitro diagnostic (IVD)-certified commercial cfDNA extraction kits showed that the microfluidic system achieved comparable cfDNA yields at standard plasma volumes and superior performance at reduced input volumes. High DNA purity and integrity were confirmed by quantitative PCR amplification of a housekeeping gene and clinically relevant targets. The complete workflow required approximately 9 min, used minimal equipment, reduced contamination risk, and enabled rapid processing with future potential for parallel multi-chip configurations. Conclusions: These findings establish the proposed microfluidic platform as a rapid, reproducible, and scalable alternative to conventional cfDNA extraction methods. By significantly improving recovery efficiency from small plasma volumes, the system enhances the clinical feasibility of liquid biopsy applications in cancer diagnostics and precision medicine.<\/jats:p>","DOI":"10.3390\/diagnostics16030460","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T14:12:46Z","timestamp":1770041566000},"page":"460","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Magnetic Nanoparticle-Integrated Microfluidic Chip Enables Reliable Isolation of Plasma Cell-Free DNA for Molecular Diagnostics"],"prefix":"10.3390","volume":"16","author":[{"given":"Amir","family":"Monfaredan","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran 14177-55469, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3967-8903","authenticated-orcid":false,"given":"Sena","family":"\u015een","sequence":"additional","affiliation":[{"name":"Department of Basic Oncology, Oncology Institute, Istanbul University, 34320 Istanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arash Adamnejad","family":"Ghafour","sequence":"additional","affiliation":[{"name":"Department of Basic Oncology, Oncology Institute, Istanbul University, 34320 Istanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5296-0009","authenticated-orcid":false,"given":"Ebru Cing\u00f6z","family":"\u00c7apan","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Techniques, Vocational School of Health Sciences, Avrasya University, 61080 Trabzon, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9398-7171","authenticated-orcid":false,"given":"Muhammed Ertu\u011frul","family":"\u00c7apan","sequence":"additional","affiliation":[{"name":"Occupational Health and Safety Program, Vocational School of Health Sciences, Avrasya University, 61080 Trabzon, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ridvan \u015ee\u00e7kin","family":"\u00d6zen","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics,  Faculty of Medicine, Beykent University, 34475 Istanbul, T\u00fcrkiye"},{"name":"Department of Internal Medical Sciences, Faculty of Medicine, Beykent University, 34475 Istanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8023-3223","authenticated-orcid":false,"given":"\u015eeref Bu\u011fra","family":"Tuncer","sequence":"additional","affiliation":[{"name":"Department of Basic Oncology, Oncology Institute, Istanbul University, 34320 Istanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oral","family":"\u00d6nc\u00fcl","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Clinical Microbiology,  Istanbul Faculty of Medicine, Istanbul University, 34320 Istanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1038\/s41392-024-02021-w","article-title":"Liquid biopsy in cancer current: Status, challenges and future prospects","volume":"9","author":"Ma","year":"2024","journal-title":"Signal Transduct. 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