{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:09:48Z","timestamp":1791317388275,"version":"4.3.1"},"reference-count":29,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background: The spleen is an important organ in the evaluation of systemic diseases due to its hematologic and immunologic functions. Changes in splenic volume and surface area have diagnostic significance in various clinical conditions. This study aimed to evaluate age- and sex-related changes in splenic volume and surface area in an adult population. Material: A total of 304 abdominal CT scans (153 females, 151 males) were retrospectively evaluated. Participants aged 19\u201389 years were stratified by decades. Changes in splenic surface area and volume were analyzed according to age and sex using measurements obtained from these images with the 3D Slicer software package. Results: Splenic volume showed significant differences across age groups in females, whereas no statistically significant age-related difference was observed in males. Splenic surface area differed significantly according to age groups in both sexes. The highest mean splenic volume and surface area were observed in the 19\u201329 age group in both sexes. The lowest mean values were detected in the 80\u201389 age group in females and in the 70\u201379 age group in males. Conclusions: Splenic volume is higher in males than in females. A statistically significant decrease in splenic volume with advancing age was observed in females, whereas only a non-significant decreasing trend was noted in males. A marked reduction in splenic volume and surface area was observed particularly after the age of 70 years. This finding is associated with age-related immune decline and functional hyposplenism and carries clinical significance in terms of increased susceptibility to infections and diminished immune response in elderly individuals. Furthermore, splenic shrinkage should be considered in the assessment of hematological reserve and in the differentiation of changes related to chronic diseases.<\/jats:p>","DOI":"10.3390\/diagnostics16091406","type":"journal-article","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T08:01:39Z","timestamp":1778140899000},"page":"1406","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of Splenic Volume and Surface Area According to Age and Gender: A Three-Dimensional Slicer Computed Tomography Study\u2014Imaging"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6927-1306","authenticated-orcid":false,"given":"Busra","family":"Pirinc","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Anatomy, Selcuk University, 42130 Konya, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9946-3026","authenticated-orcid":false,"given":"Ayse Gamze","family":"Ozcan","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Anatomy, Selcuk University, 42130 Konya, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5091-0251","authenticated-orcid":false,"given":"Ekrem","family":"Solmaz","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Anatomy, Selcuk University, 42130 Konya, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1287-5544","authenticated-orcid":false,"given":"Betul","family":"Sevindik","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Anatomy, Selcuk University, 42130 Konya, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8533-4939","authenticated-orcid":false,"given":"Emine","family":"Uysal","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Radiology, Selcuk University, 42130 Konya, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5103-090X","authenticated-orcid":false,"given":"Zeliha","family":"Fazliogullari","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Anatomy, Selcuk University, 42130 Konya, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1007\/s00261-003-0139-2","article-title":"Normal organ volume assessment from abdominal CT","volume":"29","author":"Geraghty","year":"2004","journal-title":"Abdom. Imaging"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e97","DOI":"10.1016\/j.ejrad.2009.08.013","article-title":"Splenic volume measurements on computed tomography utilizing automatically contouring software and its relationship with age, gender, and anthropometric parameters","volume":"75","author":"Harris","year":"2010","journal-title":"Eur. J. Radiol."},{"key":"ref_3","first-page":"1726","article-title":"Normal splenic volume in adults by computed tomography","volume":"49","author":"Kaneko","year":"2002","journal-title":"Hepatogastroenterology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1210\/jc.2013-1694","article-title":"Chronic starvation secondary to anorexia nervosa is associated with an adaptive suppression of resting energy expenditure","volume":"99","author":"Kosmiski","year":"2014","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1186\/s40644-023-00640-0","article-title":"Preoperative splenic area as a prognostic biomarker of early-stage non-small cell lung cancer","volume":"23","author":"Liu","year":"2023","journal-title":"Cancer Imaging"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1148\/radiol.2015150887","article-title":"Spleen size is significantly influenced by body height and sex: Establishment of normal values for spleen size at US with a cohort of 1200 healthy individuals","volume":"279","author":"Chow","year":"2016","journal-title":"Radiology"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Suttorp, M., and Classen, C.F. (2021). Splenomegaly in children and adolescents. Front. Pediatr., 9.","DOI":"10.3389\/fped.2021.704635"},{"key":"ref_8","first-page":"159","article-title":"The morphological variations of the human spleen","volume":"6","author":"Chaware","year":"2012","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1016\/j.acra.2017.06.006","article-title":"The best single measurement for assessing splenomegaly in patients with cirrhotic liver morphology","volume":"24","author":"Nuffer","year":"2017","journal-title":"Acad. Radiol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Xiong, W., Ong, S.H., Tian, Q., Xu, G., Zhou, J., Liu, J., and Venkatash, S.K. (2009). Construction of a linear unbiased diffeomorphic probabilistic liver atlas from CT images. 2009 16th IEEE International Conference on Image Processing (ICIP), IEEE.","DOI":"10.1109\/ICIP.2009.5414550"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2127","DOI":"10.1109\/TMI.2013.2274734","article-title":"Interactive medical image segmentation using PDE control of active contours","volume":"32","author":"Karasev","year":"2013","journal-title":"IEEE Trans. Med. Imaging"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1587\/transinf.E97.D.951","article-title":"An automated segmentation algorithm for CT volumes of livers with atypical shapes and large pathological lesions","volume":"97","author":"Umetsu","year":"2014","journal-title":"IEICE Trans. Inf. Syst."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.compmedimag.2015.03.002","article-title":"Granular computing in model based abdominal organs detection","volume":"46","author":"Juszczyk","year":"2015","journal-title":"Comput. Med. Imaging Graph."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/j.mri.2012.05.001","article-title":"3D slicer as an image computing platform for the quantitative imaging network","volume":"30","author":"Fedorov","year":"2012","journal-title":"Magn. Reson. Imaging"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e230024","DOI":"10.1148\/ryai.230024","article-title":"Total Segmentator: Robust segmentation of 104 anatomic structures in CT images","volume":"5","author":"Wasserthal","year":"2023","journal-title":"Radiol. Artif. Intell."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1007\/s00276-012-0988-x","article-title":"Age-related volumetric changes in pancreas: A stereological study on computed tomography","volume":"34","author":"Caglar","year":"2012","journal-title":"Surg. Radiol. Anat."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"52","DOI":"10.5395\/rde.2013.38.1.52","article-title":"Statistical notes for clinical researchers: Assessing normal distribution (2) usings kewness and kurtosis","volume":"37","author":"Kim","year":"2013","journal-title":"Restor. Dent. Endod."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1097\/MCG.0b013e31825ceeb5","article-title":"Prognostic significance of computed tomography scan-derived splenic volume in the patocellular carcinoma treated with radiofrequen cy ablation","volume":"46","author":"Wu","year":"2012","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Paley, M.R., and Ros, P.R. (2002). Imaging of spleen disorders. The Complete Spleen: Structure, Function, and Clinical Disorders, Humana Press.","DOI":"10.1007\/978-1-59259-124-4_16"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.compbiomed.2019.01.018","article-title":"Acceleration of spleen segmentation with end-to-end deep learning method and automated pipeline","volume":"107","author":"Moon","year":"2019","journal-title":"Comput. Biol. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1258\/ar.2011.100499","article-title":"The optimal contrast media policy in CT of the liver. Part I: Technical notes","volume":"52","author":"Rengo","year":"2011","journal-title":"Acta Radiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/s10140-016-1402-5","article-title":"Spleen volume on CT and the effect of abdominal trauma","volume":"23","author":"Agarwal","year":"2016","journal-title":"Emerg. Radiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2958","DOI":"10.1016\/j.jstrokecerebrovasdis.2016.08.012","article-title":"The volume of the spleen and its correlates after acute stroke","volume":"25","author":"Chiu","year":"2016","journal-title":"J. Stroke Cerebrovasc. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1148\/radiol.2021204183","article-title":"Population-based and personalized reference intervals for liver and spleen volumes in healthy individuals and those with viral hepatitis","volume":"301","author":"Kim","year":"2021","journal-title":"Radiology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1007\/s11604-019-00841-3","article-title":"Postmortem volume change of the spleen and kidney on early postmortem computed tomography: Comparison with antemortem computed tomography","volume":"37","author":"Takahashi","year":"2019","journal-title":"Jpn. J. Radiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e28515","DOI":"10.1097\/MD.0000000000028515","article-title":"Evaluation of the relationship between the spleen volume and the disease activity in ulcerative colitis and Crohn disease","volume":"101","author":"Kawashima","year":"2022","journal-title":"Medicine"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s40477-025-01107-7","article-title":"Ellipsoid vs spherical cap: A new approach to splenic volumetry validated on cadavers","volume":"29","author":"Anzoletti","year":"2026","journal-title":"J. Ultrasound"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"183","DOI":"10.5603\/FM.2014.0027","article-title":"Estimation of spleen volume and surface area of the newborns\u2019 cadaveric spleen using stereological methods","volume":"73","author":"Sagiroglu","year":"2014","journal-title":"Folia Morphol."},{"key":"ref_29","first-page":"11","article-title":"Correlation of Morphometric Parameters of Spleen and Height of the Deceased: A Cross-sectional Study in North Indian Population","volume":"16","author":"Dang","year":"2022","journal-title":"J. Clin. Diagn. Res."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/16\/9\/1406\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T08:28:36Z","timestamp":1778142516000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/16\/9\/1406"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,6]]},"references-count":29,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["diagnostics16091406"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics16091406","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,6]]}}}