{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:29:30Z","timestamp":1785432570060,"version":"3.56.0"},"reference-count":22,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003052","name":"Ministry of Trade, Industry and Energy","doi-asserted-by":"crossref","award":["RS-2024-00403563"],"award-info":[{"award-number":["RS-2024-00403563"]}],"id":[{"id":"10.13039\/501100003052","id-type":"DOI","asserted-by":"crossref"}]},{"award":["RS-2024-00403563"],"award-info":[{"award-number":["RS-2024-00403563"]}],"id":[{"id":"https:\/\/ror.org\/008nkqk13","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Hoilbiomed"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background\/Objectives: We evaluated the diagnostic performance of the Alifax Molecular Mouse (MM) sepsis panel for rapidly directly detecting pathogens and resistance genes in positive blood culture broths. Methods: In this prospective single-center study, 40 positive blood culture specimens were analyzed using four MM cartridges targeting Gram-positive and -negative bacteria and major antimicrobial resistance genes. The results were compared with those obtained using the routine microbiological protocol, including organism identification using the MALDI Biotyper system and antimicrobial susceptibility testing using the VITEK 2 and MicroScan WalkAway systems. Additionally, the MM panel results were compared with those obtained using the FilmArray BCID2. Results: Compared with the routine protocol, the MM panel achieved a positive percent agreement (PPA) of 89.5% (34\/38; 95% confidence interval [CI], 75.9\u201395.8%) for bacterial identification, indicating moderate-to-good diagnostic performance. For antimicrobial resistance gene detection, the PPA was 92.1% (35\/38; 95% CI, 79.2\u201397.3%). Compared with the FilmArray BCID2, the overall agreement of the MM panel was 89.4% (34\/38) for bacterial identification and 84.2% (32\/38) for antimicrobial resistance gene detection. The MM panel generated clinically actionable results within 1 h (mean turnaround time, 55.5 min; 95% CI, 55.1\u201355.9 min), substantially reducing the turnaround time compared with the conventional microbiological workflow, which requires 1.5\u20132 days for subculture and automated antimicrobial susceptibility testing. Conclusions: The MM sepsis panel enabled rapid, direct identification of bloodstream pathogens and key antimicrobial resistance markers from positive blood cultures, substantially reducing turnaround time and exhibiting its potential as an adjunct to routine microbiological workflows.<\/jats:p>","DOI":"10.3390\/diagnostics16152369","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T08:52:01Z","timestamp":1785228721000},"page":"2369","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preliminary Single-Center Evaluation of the Alifax Molecular Mouse Sepsis Panel for Rapid Detection of Bloodstream Pathogens"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5874-3144","authenticated-orcid":false,"given":"Jay Ho","family":"Han","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Uijeongbu St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Gyeonggi-do 11765, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0928-9109","authenticated-orcid":false,"given":"Jae Kwon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Ok","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Eunpyeong St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 03312, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Suk","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Eunpyeong St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 03312, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5216-7732","authenticated-orcid":false,"given":"Sungjin","family":"Jo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Eunpyeong St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Seoul 03312, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"R613","DOI":"10.1152\/ajpregu.00003.2023","article-title":"Sepsis: Network pathophysiology and implications for early diagnosis","volume":"324","author":"Arora","year":"2023","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1164\/rccm.201609-1848OC","article-title":"The Timing of Early Antibiotics and Hospital Mortality in Sepsis","volume":"196","author":"Liu","year":"2017","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1007\/s00134-021-06506-y","article-title":"Surviving sepsis campaign: International guidelines for management of sepsis and septic shock 2021","volume":"47","author":"Evans","year":"2021","journal-title":"Intensive Care Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0025-6196(25)09635-1","article-title":"Blood cultures: Principles and techniques","volume":"50","author":"Washington","year":"1975","journal-title":"Mayo Clin. Proc."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Roberts, T., Chandna, A., Watthanaworawit, W., Thaiprakong, A., Soeng, S., Simmalavong, M., Phoumin, P., Saengchun, W., Khatta, N., and Hinfonthong, P. (2022). Impact of delayed processing of positive blood cultures on organism detection: A prospective multi-centre study. BMC Infect. Dis., 22.","DOI":"10.1186\/s12879-022-07504-1"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1080\/14737159.2019.1660644","article-title":"Diagnosis of bacterial sepsis: Why are tests for bacteremia not sufficient?","volume":"19","author":"Sweeney","year":"2019","journal-title":"Expert Rev. Mol. Diagn."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1097\/CCM.0000000000000330","article-title":"Empiric antibiotic treatment reduces mortality in severe sepsis and septic shock from the first hour: Results from a guideline-based performance improvement program","volume":"42","author":"Ferrer","year":"2014","journal-title":"Crit. Care Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.diagmicrobio.2015.10.023","article-title":"Clinical and economic impact of antimicrobial stewardship interventions with the FilmArray blood culture identification panel","volume":"84","author":"Pardo","year":"2016","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1128\/CMR.00016-12","article-title":"New Approaches to Sepsis: Molecular Diagnostics and Biomarkers","volume":"25","author":"Reinhart","year":"2012","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/cid\/ciw649","article-title":"The Effect of Molecular Rapid Diagnostic Testing on Clinical Outcomes in Bloodstream Infections: A Systematic Review and Meta-analysis","volume":"64","author":"Timbrook","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e00024-17","DOI":"10.1128\/CMR.00024-17","article-title":"Syndromic Panel-Based Testing in Clinical Microbiology","volume":"31","author":"Ramanan","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Mauri, C., Consonni, A., Briozzo, E., Giubbi, C., Meroni, E., Tonolo, S., and Luzzaro, F. (2024). The Molecular Mouse System: A New Useful Tool for Guiding Antimicrobial Therapy in Critically Ill Septic Patients. Antibiotics, 13.","DOI":"10.3390\/antibiotics13060517"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e25-00006","DOI":"10.1097\/JTCCM-D-25-00006","article-title":"Next-generation sequencing vs. traditional pathogen culture methods in the diagnosis of severe infections in intensive care unit patients: A comparative study","volume":"7","author":"Wang","year":"2025","journal-title":"J. Transl. Crit. Care Med."},{"key":"ref_14","unstructured":"Clinical and Laboratory Standards Institute (2011). Performance Standards for Antimicrobial Susceptibility Testing: 20th Informational Supplement, Clinical and Laboratory Standards Institute. CLSI Document M100-S21."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Founou, R.C., Founou, L.L., and Essack, S.Y. (2017). Clinical and economic impact of antibiotic resistance in developing countries: A systematic review and meta-analysis. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0189621"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1179\/2047773215Y.0000000030","article-title":"Antimicrobial resistance: A global multifaceted phenomenon","volume":"109","author":"Prestinaci","year":"2015","journal-title":"Pathog. Glob. Health"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/AAC.00355-19","article-title":"The Microbiology of Bloodstream Infection: 20-Year Trends from the SENTRY Antimicrobial Surveillance Program","volume":"63","author":"Diekema","year":"2019","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1866","DOI":"10.1128\/JCM.00376-11","article-title":"Nosocomial bloodstream infections in Brazilian hospitals: Analysis of 2563 cases from a prospective nationwide surveillance study","volume":"49","author":"Marra","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1146\/annurev-anchem-061622-015112","article-title":"The Present and Future Landscapes of Molecular Diagnostics","volume":"17","author":"Rolando","year":"2024","journal-title":"Annu. Rev. Anal. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"106190","DOI":"10.1016\/j.prevetmed.2024.106190","article-title":"Accuracy of molecular diagnostic assays for detection of Mycobacterium bovis: A systematic review and meta-analysis","volume":"226","author":"Mabe","year":"2024","journal-title":"Prev. Vet. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2539","DOI":"10.21037\/jtd-24-400","article-title":"Etiological diagnostic performance of probe capture-based targeted next-generation sequencing in bloodstream infection","volume":"16","author":"Cai","year":"2024","journal-title":"J. Thorac. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Colosimo, C., Montanari, M.S., Marzucco, A., Gatti, G., Puccini, L., Grumiro, L., Dionisi, L., Brandolini, M., Ingletto, L., and Guerra, M. (2025). Evaluation of molecular mouse sepsis panel: New portable and rapid tests for microorganism detection in suspected blood stream infection. Front. Cell. Infect. 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