{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T09:33:24Z","timestamp":1787045604981,"version":"build-2736575974"},"reference-count":47,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007935","name":"Academic Enhancement Support Program of Hainan Medical University","doi-asserted-by":"publisher","award":["XSTS2025105"],"award-info":[{"award-number":["XSTS2025105"]}],"id":[{"id":"10.13039\/501100007935","id-type":"DOI","asserted-by":"publisher"}]},{"award":["XSTS2025105"],"award-info":[{"award-number":["XSTS2025105"]}],"id":[{"id":"https:\/\/ror.org\/004eeze55","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008111","name":"Hainan Province Science and Technology Special Fund","doi-asserted-by":"publisher","award":["ZDYF2022SHFZ050"],"award-info":[{"award-number":["ZDYF2022SHFZ050"]}],"id":[{"id":"10.13039\/501100008111","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Natural Science Fund Cultivating Project of Hainan General Hospital","award":["2022MSXM02"],"award-info":[{"award-number":["2022MSXM02"]}]},{"award":["2022MSXM02"],"award-info":[{"award-number":["2022MSXM02"]}],"id":[{"id":"https:\/\/ror.org\/030sr2v21","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Melioidosis, caused by Burkholderia pseudomallei, is a severe infectious disease with high mortality. Diagnostic delays due to conventional culture and serology limitations impact patient outcomes. This narrative review synthesizes evidence on next-generation sequencing (NGS) in melioidosis. NGS technologies encompass two main applications: metagenomic NGS (mNGS), which enables culture-independent detection directly from clinical samples, and whole-genome sequencing (WGS), which provides outbreak tracing and source attribution from cultured isolates. Resistance profiling detects antimicrobial resistance (AMR) determinants (e.g., penA mutations) to guide therapy. Recent 2025\u20132026 studies highlight new applications, including direct pathogen genome recovery from environmental samples. Despite cost and standardization challenges, integrating NGS into clinical workflows holds promise for improving melioidosis management, especially in resource-limited settings.<\/jats:p>","DOI":"10.3390\/diagnostics16162613","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T09:06:41Z","timestamp":1787044001000},"page":"2613","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Next-Generation Sequencing in Melioidosis: Enhancing Diagnosis, Epidemiology and Antimicrobial Resistance Surveillance"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9140-0864","authenticated-orcid":false,"given":"Hua","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Hainan Women and Children\u2019s Medical Center, Haikou 570206, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0081-5678","authenticated-orcid":false,"given":"Pei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Hainan General Hospital, Haikou 570311, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Hainan General Hospital, Haikou 570311, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Affiliated Hainan Hospital, Hainan Medical University, Haikou 570102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/s41579-023-00972-5","article-title":"Burkholderia pseudomallei and melioidosis","volume":"22","author":"Meumann","year":"2024","journal-title":"Nat. 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