{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:27:59Z","timestamp":1787128079610,"version":"build-2736575974"},"reference-count":36,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Medicine and Pharmacy Carol Davila"},{"id":[{"id":"https:\/\/ror.org\/04fm87419","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background\/Objectives: The genital tubercle develops in the fourth week from mesenchymal cells in the lateral edges of the embryo. In females the genital tubercle develops into the clitoris while in males it participates in forming the penis. The aim of this study was to perform a macroscopic as well as an ultrasonographic assessment of the genital tubercle and its gender-dependent derivates, presenting a parallel comparative evaluation between the two. Methods: We performed a morphological assessment of the genital tubercle through micro-dissection of 141 embryos and fetuses with gestational ages between 5 and 14 weeks, in the Department of Anatomy of \u201cCarol Davila\u201d University of Medicine and Pharmacy. An ultrasound imaging study of the genital tubercle using 3-dimensional ultrasonography (3DUS) was also performed in the Department of Obstetrics and Gynecology of University Emergency Hospital of Bucharest. The morphology of the genital tubercle was analyzed by ultrasound scanning of 119 embryos and fetuses with gestational ages between 5 and 14 weeks. The age of all the embryos was determined by measuring the crown\u2013rump length. Results: The dimensions and especially the direction of the genital tubercle were evaluated and compared in order to establish whether sex determination is possible as early as 7\u20138 weeks of gestation. The genital tubercle orientates cranially in males and caudally in females as early as 6 weeks of gestation, during the bi-potential phase. Conclusions: The orientation of the genital tubercle plays an essential role in establishing the sex of the fetus, regardless of its size.<\/jats:p>","DOI":"10.3390\/diagnostics16162626","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T07:58:23Z","timestamp":1787126303000},"page":"2626","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Morphology of the Genital Tubercle: A Macroscopic Assessment and Ultrasound Approach on Early Fetal Sex Determination"],"prefix":"10.3390","volume":"16","author":[{"given":"Octavian","family":"Munteanu","sequence":"first","affiliation":[{"name":"Department of Anatomy, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica-Mihaela","family":"Cirstoiu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roxana","family":"Bohiltea","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Stomatology, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andra-Ioana","family":"Baloiu","sequence":"additional","affiliation":[{"name":"Department of Anatomy, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Voinea","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Razvan-Adrian","family":"Covache-Busuioc","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"},{"name":"Puls Med Association, 051885 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matei","family":"Serban","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"},{"name":"Puls Med Association, 051885 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Tulin","sequence":"additional","affiliation":[{"name":"Department of Anatomy, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iulian","family":"Slavu","sequence":"additional","affiliation":[{"name":"Department of Anatomy, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5054-4949","authenticated-orcid":false,"given":"Zoran","family":"Filipoiu","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioan-Andrei","family":"Petrescu","sequence":"additional","affiliation":[{"name":"Department of Anatomy, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2043-9307","authenticated-orcid":false,"given":"Ciprian","family":"Coroleuca","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1038\/s41585-023-00754-x","article-title":"Genetic Control of Typical and Atypical Sex Development","volume":"20","author":"Reyes","year":"2023","journal-title":"Nat. Rev. Urol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Deegan, D.F., and Engel, N. (2019). Sexual Dimorphism in the Age of Genomics: How, When, Where. Front. Cell Dev. Biol., 7.","DOI":"10.3389\/fcell.2019.00186"},{"key":"ref_3","first-page":"261","article-title":"Molecular Mechanisms of External Genitalia Development","volume":"84","author":"Blaschko","year":"2012","journal-title":"Differ. Res. Biol. Divers."},{"key":"ref_4","first-page":"74","article-title":"Development of the Human Penis and Clitoris","volume":"103","author":"Baskin","year":"2018","journal-title":"Differ. Res. Biol. Divers."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.diff.2018.08.002","article-title":"Human Glans and Preputial Development","volume":"103","author":"Liu","year":"2018","journal-title":"Differentiation"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"71","DOI":"10.4103\/0300-1652.103545","article-title":"The Accuracy of 2D Ultrasound Prenatal Sex Determination","volume":"53","author":"Igbinedion","year":"2012","journal-title":"Niger. Med. J. J. Niger. Med. Assoc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"210","DOI":"10.4103\/jfmpc.jfmpc_180_17","article-title":"The Ultrasound Identification of Fetal Gender at the Gestational Age of 11\u201312 Weeks","volume":"7","author":"Gharekhanloo","year":"2018","journal-title":"J. Fam. Med. Prim. Care"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s12610-023-00202-1","article-title":"Suspension of the Penis\u2014Dissection, Anatomical Description and Highlighting of Anatomical Risks in Sectioning the Suspensory Ligaments","volume":"33","author":"Filipoiu","year":"2023","journal-title":"Basic Clin. Androl."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1111\/joa.14150","article-title":"The Development of the External Genitals in Female Human Embryos and Foetuses. Part 2: Vaginal Vestibule, Anal Canal, Perineal Raphe and Perineal Cutaneous Muscles","volume":"246","author":"Gao","year":"2025","journal-title":"J. Anat."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"654.e1","DOI":"10.1016\/j.jpurol.2020.07.015","article-title":"Are Anogenital Distance and External Female Genitalia Development Changed in Neural Tube Defects? Study in Human Fetuses","volume":"16","author":"Vieiralves","year":"2020","journal-title":"J. Pediatr. Urol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1093\/humrep\/dey265","article-title":"Anogenital Distance Is Determined during Early Gestation in Humans","volume":"33","author":"Jain","year":"2018","journal-title":"Hum. Reprod."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1111\/joa.14139","article-title":"The development of the external genitals in female human embryos and foetuses. Part 1: Perineal thick skin, clitoris and labia","volume":"246","author":"Gao","year":"2025","journal-title":"J. Anat."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"409","DOI":"10.7863\/jum.2012.31.3.409","article-title":"Fetal transient clitoromegaly and transient hypertrophy of the labia minora in early and mid pregnancy","volume":"31","author":"Zimmer","year":"2012","journal-title":"J. Ultrasound Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1159\/000489385","article-title":"Clitoromegaly in childhood and adolescence: Behind one clinical sign, a clinical sea","volume":"12","author":"Iezzi","year":"2018","journal-title":"Sex. Dev."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Mitani-Konno, M., Saito, R., Narumi-Wakayama, H., Sakai, Y., Suzuki, S., Satoh, H., and Hasegawa, Y. (2023). Clitoral preputial edema can be mistaken for clitoromegaly: A clinical analysis of ten cases. Front. Endocrinol., 14.","DOI":"10.3389\/fendo.2023.1175611"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1016\/j.jpurol.2013.03.001","article-title":"Persistent unexplained congenital clitoromegaly in females born extremely prematurely","volume":"9","author":"Williams","year":"2013","journal-title":"J. Pediatr. Urol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1046\/j.1469-0705.1999.13050305.x","article-title":"First-Trimester Determination of Fetal Gender by Ultrasound","volume":"13","author":"Efrat","year":"1999","journal-title":"Ultrasound Obstet. Gynecol. Off. J. Int. Soc. Ultrasound Obstet. Gynecol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1046\/j.1469-0705.1999.13050308.x","article-title":"Biometrical Threshold of Biparietal Diameter for Certain Fetal Sex Assignment by Ultrasound","volume":"13","author":"Mazza","year":"1999","journal-title":"Ultrasound Obstet. Gynecol. Off. J. Int. Soc. Ultrasound Obstet. Gynecol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1002\/pd.945","article-title":"Sonographic Biometrical Range of External Genitalia Differentiation in the First Trimester of Pregnancy: Analysis of 2593 Cases","volume":"24","author":"Mazza","year":"2004","journal-title":"Prenat. Diagn."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1007\/s00404-022-06441-3","article-title":"Clinical Spectrum of Female Genital Malformations in Prenatal Diagnosis","volume":"306","author":"Mallmann","year":"2022","journal-title":"Arch. Gynecol. Obstet."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/0002-9378(87)90055-X","article-title":"Can Ultrasonography Replace Amniocentesis in Fetal Gender Determination during the Early Second Trimester?","volume":"156","author":"Reece","year":"1987","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_22","first-page":"247","article-title":"Early-Second-Trimester Fetal Sex Determination with Ultrasound","volume":"35","author":"Watson","year":"1990","journal-title":"J. Reprod. Med."},{"key":"ref_23","first-page":"293","article-title":"The Sagittal Sign. An Early Second Trimester Sonographic Indicator of Fetal Gender","volume":"8","author":"Emerson","year":"1989","journal-title":"J. Ultrasound Med. Off. J. Am. Inst. Ultrasound Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1046\/j.1469-0705.1996.08050322.x","article-title":"Early Second-Trimester Determination of Fetal Gender by Ultrasound","volume":"8","author":"Meagher","year":"1996","journal-title":"Ultrasound Obstet. Gynecol. Off. J. Int. Soc. Ultrasound Obstet. Gynecol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Kim, M.G., Yoon, C., and Lim, H.G. (2024). Recent Advancements in High-Frequency Ultrasound Applications from Imaging to Microbeam Stimulation. Sensors, 24.","DOI":"10.3390\/s24196471"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1002\/jcu.1870180414","article-title":"Early Determination of Fetal Sex Using Transvaginal Sonography: Technique and Pitfalls","volume":"18","author":"Bronshtein","year":"1990","journal-title":"J. Clin. Ultrasound JCU"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1080\/00016340701571905","article-title":"Fetal gender screening by ultrasound at 11 to 13+ 6 weeks","volume":"87","author":"Hsiao","year":"2008","journal-title":"Acta Obstet. Gynecol. Scand."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Alfuraih, A.M., Almajem, B.M., and Alsolai, A.A. (2024). The Predictive Accuracy of Anogenital Distance and Genital Tubercle Angle for First-Trimester Fetal Sex Determination. Diagnostics, 14.","DOI":"10.3390\/diagnostics14161811"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1007\/s40477-024-00977-7","article-title":"Effectiveness of prenatal ultrasound in fetal sex identification: A systematic review and meta-analysis","volume":"28","author":"Amankona","year":"2025","journal-title":"J. Ultrasound"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1002\/jum.15821","article-title":"Feasibility analysis of 3D printing with prenatal ultrasound for the diagnosis of fetal abnormalities","volume":"41","author":"Liang","year":"2022","journal-title":"J. Ultrasound Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"195","DOI":"10.7863\/jum.2010.29.2.195","article-title":"Use of Multiplanar 3-Dimensional Ultrasonography for Prenatal Sex Identification","volume":"29","author":"Hackett","year":"2010","journal-title":"J. Ultrasound Med."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Mlodawski, J., Zmelonek-Znamirowska, A., Pawlik, L., Mlodawska, M., and Swiercz, G. (2025). Reproducibility Challenges in Fetal Cardiac Function Analysis with 2D Speckle-Tracking Echocardiography: Insights from FetalHQ. J. Clin. Med., 14.","DOI":"10.3390\/jcm14103301"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1002\/uog.8973","article-title":"Female External Genitalia on Fetal Magnetic Resonance Imaging","volume":"38","author":"Nemec","year":"2011","journal-title":"Ultrasound Obstet. Gynecol. Off. J. Int. Soc. Ultrasound Obstet. Gynecol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1177\/1742271X16651901","article-title":"Helping expectant mothers understand inadequate ultrasound images","volume":"24","author":"Oates","year":"2016","journal-title":"Ultrasound"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Timor-Tritsch, I.E., and Monteagudo, A. (2009). Scanning techniques in obstetrics and gynaecology. Ultrasound in Obstetrics and Gynaecology, Elsevier.","DOI":"10.1016\/B978-0-444-51829-3.00003-9"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"25076","DOI":"10.1038\/s41598-024-77313-w","article-title":"Repeatability and reproducibility of artificial intelligence-acquired fetal brain measurements (SonoCNS) in the second and third trimesters of pregnancy","volume":"14","author":"Mlodawski","year":"2024","journal-title":"Sci. Rep."}],"container-title":["Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4418\/16\/16\/2626\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:12:03Z","timestamp":1787127123000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4418\/16\/16\/2626"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":36,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["diagnostics16162626"],"URL":"https:\/\/doi.org\/10.3390\/diagnostics16162626","relation":{},"ISSN":["2075-4418"],"issn-type":[{"value":"2075-4418","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]}}}