{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T09:23:40Z","timestamp":1787304220318,"version":"build-2736575974"},"reference-count":20,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science Committee of the Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["BR24992881"],"award-info":[{"award-number":["BR24992881"]}]},{"award":["BR24992881"],"award-info":[{"award-number":["BR24992881"]}],"id":[{"id":"https:\/\/ror.org\/05tyne317","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background\/Objectives: Human adenoviruses (HAdVs) are widely used as vectors for vaccines and gene therapy; however, pre-existing immunity can reduce their efficacy. Therefore, rapid and accessible serological methods are required to assess antibody levels against adenoviruses. Lateral flow immunochromatographic assay (LFIA) sensitivity depends on the label and antigen. In this study, we aimed to develop and evaluate LFIA systems based on gold nanoparticles (GNPs) and quantum dots (QDs) using a recombinant HAdV hexon protein. Methods: A recombinant HAdV hexon protein fragment (rhHAdV, 35 kDa; amino acids A120-R316) was expressed in Escherichia coli and purified using Ni2+ affinity chromatography. Protein identity was confirmed using sodium dodecyl sulfate\u2013polyacrylamide gel electrophoresis, Western blotting, and liquid chromatography\u2013tandem mass spectrometry analyses. Two LFIA formats were developed using Protein G-conjugated GNPs (GNP-G) and QDs (QD-G). Agreement with the reference ELISA was evaluated using 90 human serum samples. Results: The rhHAdV antigen demonstrated reactivity with anti-adenovirus-positive human sera in ELISA. Antibody detection was achieved at serum dilutions of up to 1:300 and 1:1000 for the GNP- and QD-based LFIA, respectively. The GNP-G and QD-G LFIA formats demonstrated positive percent agreements of 96.9% and 100%, respectively, while both assays showed a negative percent agreement of 98.2% compared with the reference ELISA. Conclusions: The findings demonstrate the feasibility of a QD-based LFIA for qualitative detection of antibodies reactive with the recombinant HAdV-B3 hexon fragment.<\/jats:p>","DOI":"10.3390\/diagnostics16162670","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T08:14:01Z","timestamp":1787300041000},"page":"2670","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lateral Flow Immunochromatographic Assay for Detection of Antibodies Against a Recombinant Human Adenovirus Type 3 Hexon Fragment Using Gold Nanoparticles and Quantum Dots"],"prefix":"10.3390","volume":"16","author":[{"given":"Kanatbek","family":"Mukantayev","sequence":"first","affiliation":[{"name":"Laboratory of Immunochemistry and Immunobiotechnology, National Center of Biotechnology, 13\/5, Kurgalzhynskoye Road, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanat","family":"Tursunov","sequence":"additional","affiliation":[{"name":"Laboratory of Immunochemistry and Immunobiotechnology, National Center of Biotechnology, 13\/5, Kurgalzhynskoye Road, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Tokhtarova","sequence":"additional","affiliation":[{"name":"Laboratory of Immunochemistry and Immunobiotechnology, National Center of Biotechnology, 13\/5, Kurgalzhynskoye Road, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bisultan","family":"Abirbekov","sequence":"additional","affiliation":[{"name":"Laboratory of Immunochemistry and Immunobiotechnology, National Center of Biotechnology, 13\/5, Kurgalzhynskoye Road, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1038\/s41591-021-01677-z","article-title":"Heterologous AD5-nCOV plus CoronaVac versus homologous CoronaVac vaccination: A randomized phase 4 trial","volume":"28","author":"Li","year":"2022","journal-title":"Nat. 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