{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:06:47Z","timestamp":1790701607460,"version":"4.1.0"},"reference-count":26,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Background\/Objectives: Direct comparisons between commercial molecular platforms for vaginitis diagnosis are scarce. We compared the Aptima BV and CV\/TV assays (Hologic) with the Vaginal Real Time kit (Vircell) for bacterial vaginosis (BV), Candida spp. and Trichomonas vaginalis, and explored the association between BV and sexually transmitted infection (STI) co-detection. Methods: In this prospective single-centre study, 350 vaginal swabs submitted for molecular STI screening were tested on both platforms. Discordances were arbitrated with the Allplex\u2122 Vaginitis Screening assay (Seegene) within a composite reference standard (CRS). Agreement was assessed with Cohen\u2019s \u03ba and positive\/negative percentage agreement (PPA\/NPA); performance was calculated against the CRS. Results: Agreement was substantial for BV (\u03ba = 0.676; PPA 94.8%, NPA 79.1%) and almost perfect for Candida spp. (\u03ba = 0.885) and T. vaginalis (\u03ba = 0.920), in line with previous molecular-versus-Nugent comparisons. Vircell classified more samples as BV-positive than Aptima (45.4% vs. 33.1%; p &lt; 0.001); the Aptima BV positivity rate closely matched the 34% reported by Vipond et al. Against the CRS, Aptima showed higher specificity (98.7%) and Vircell higher sensitivity (98.3%). Agreement fell to 75.9% in women aged \u226545 years. BV positivity was associated with STI co-detection, strongest for Chlamydia trachomatis (OR 8.97, 95% CI 3.26\u201324.71). Conclusions: The two platforms performed equivalently for candidiasis and trichomoniasis but diverged for BV, particularly in older women, reflecting their different classification algorithms rather than analytical quality.<\/jats:p>","DOI":"10.3390\/diagnostics16193173","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:33:12Z","timestamp":1790699592000},"page":"3173","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative\u00a0Performance of Aptima BV\/CV-TV and Vaginal Real Time Assays for the Molecular Diagnosis of Bacterial Vaginosis and Vaginitis"],"prefix":"10.3390","volume":"16","author":[{"given":"Laura","family":"Gozalo \u00c1lvarez","sequence":"first","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Cl\u00ednico San Cecilio, 18016\u00a0Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1886-3175","authenticated-orcid":false,"given":"Luc\u00eda","family":"P\u00e9rez Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Cl\u00ednico San Cecilio, 18016\u00a0Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria IBS.GRANADA, 18012 Granada, Spain"},{"name":"CIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III,\u00a028029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elvira","family":"Garc\u00eda-Valdecasas Garc\u00eda","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Cl\u00ednico San Cecilio, 18016\u00a0Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Fuentes L\u00f3pez","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Cl\u00ednico San Cecilio, 18016\u00a0Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria IBS.GRANADA, 18012 Granada, Spain"},{"name":"CIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III,\u00a028029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4318-858X","authenticated-orcid":false,"given":"Adolfo","family":"de Salazar","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Cl\u00ednico San Cecilio, 18016\u00a0Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria IBS.GRANADA, 18012 Granada, Spain"},{"name":"CIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III,\u00a028029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7611-781X","authenticated-orcid":false,"given":"Federico","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Cl\u00ednico San Cecilio, 18016\u00a0Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria IBS.GRANADA, 18012 Granada, Spain"},{"name":"CIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III,\u00a028029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"ref_1","first-page":"321","article-title":"Vaginitis: Diagnosis and Treatment","volume":"97","author":"Paladine","year":"2018","journal-title":"Am. 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