{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T02:19:43Z","timestamp":1767665983401,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T00:00:00Z","timestamp":1565049600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"DivisionDivision of Intramural Research, National Institute of Dental and Craniofacial Research.","award":["Z-9999"],"award-info":[{"award-number":["Z-9999"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Point-of-care tests are needed for the screening of head and neck squamous cell carcinoma (HNSCC) and other malignancies. Luciferase immunoprecipitation systems (LIPS), employing light-emitting proteins, were used to examine serum antibodies against several cancer-associated targets in blood donor controls and subjects with colon cancer (CC) and HNSCC. The assessment of antibodies against the wild type p53 tumor antigen showed that approximately 25% of the CC and 20% of the HNSCC patients were seropositive. In addition, humoral responses against two p53 mutants, p53-R175H and p53-R273H, generally tracked the antibody responses seen against wild type p53. Analysis of antibodies against highly specific biomarkers of HPV-16-associated malignancy, E2, E6, and E7 oncoproteins, revealed no seropositivity in blood donors and CC patients. However, 45% (9\/20) of the HNSCC patients showed E6 seropositivity, which overlapped all the detectable E2 (40%; 8\/20) and E7 seropositive subjects (35%; 7\/20). Using neodymium magnets, ultrarapid LIPSTICKS testing of HPV-16 E6 antibodies in &lt;60 s per HNSCC sample demonstrated almost the same diagnostic performance (40% sensitivity and 100% specificity) as LIPS testing in 2.5 h. While additional improvements and standardization are needed, these results highlight the possibility of using these approaches for the diagnosis of HPV-16-associated HNSCC.<\/jats:p>","DOI":"10.3390\/diagnostics9030089","type":"journal-article","created":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T03:09:08Z","timestamp":1565147348000},"page":"89","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Luciferase-Based Detection of Antibodies for the Diagnosis of HPV-Associated Head and Neck Squamous Cell Carcinoma"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1717-048X","authenticated-orcid":false,"given":"Peter D.","family":"Burbelo","sequence":"first","affiliation":[{"name":"Dental Clinical Research Core, National Institutes of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrija","family":"Chaturvedi","sequence":"additional","affiliation":[{"name":"Dental Clinical Research Core, National Institutes of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abner L.","family":"Notkins","sequence":"additional","affiliation":[{"name":"Experimental Medicine Section, National Institutes of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sreenivasulu","family":"Gunti","sequence":"additional","affiliation":[{"name":"Experimental Medicine Section, National Institutes of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1080\/14737159.2017.1404906","article-title":"Serum, plasma and saliva biomarkers for head and neck cancer","volume":"18","author":"Arantes","year":"2018","journal-title":"Expert Rev. 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