{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T22:22:21Z","timestamp":1774390941943,"version":"3.50.1"},"reference-count":19,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministerul Educa\u021biei \u0219i Cercet\u0103rii \u0218tiin\u021bifice","award":["PD_367"],"award-info":[{"award-number":["PD_367"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostics"],"abstract":"<jats:p>Therapeutic neoangiogenesis (TNA) holds promise as a treatment for peripheral arterial disease. Nevertheless, proper tools for in vivo pre-clinical investigation of different TNA approaches and their effects are still lacking. Here we describe a chronic ischemic hindlimb model in rats using laser Doppler quantitative evaluation of tissue perfusion. Male Wistar rats (n = 20), aged between 6\u20138 months, with an average weight of 287 \u00b1 26.74 g, were used. Animals were divided into two experimental groups: group A (n = 17; hindlimb chronic ischemia model) and group B (n = 3; control). Hindlimb ischemia was induced by concomitant ligation of the right femoral and popliteal artery. Evaluation of tissue perfusion was quantified in perfusion units (PU) on a scale from 0 to 500 (500 PU = maximal detectable perfusion) by laser Doppler analysis at day 0, day 15 and day 30 after induction of ischemia. Induction of chronic ischemia in the rat hindlimb by concomitant ligation of the femoral and popliteal artery can be readily obtained but requires basic microsurgical skills. Laser Doppler analysis has shown unaltered ischemia levels throughout the study (129,17 PU \u00b1 3.13 day 0 vs. 130,33 PU day 30 \u00b1 3,27, p = not significant (n.s.)). We demonstrate a simple and reproducible model of chronic hindlimb ischemia in rats, with stable tissue perfusion levels that are accurately quantified using laser Doppler technology. Hence, this model can represent a valid tool for further studies involving therapeutic neoangiogenesis.<\/jats:p>","DOI":"10.3390\/diagnostics9040139","type":"journal-article","created":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T08:17:54Z","timestamp":1570004274000},"page":"139","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Chronic Hindlimb Ischemia Assessment; Quantitative Evaluation Using Laser Doppler in a Rodent Model of Surgically Induced Peripheral Arterial Occlusion"],"prefix":"10.3390","volume":"9","author":[{"given":"Bogdan","family":"Hoinoiu","sequence":"first","affiliation":[{"name":"Division of Clinical Practical Skills, Victor Babes University of Medicine and Pharmacy, Timisoara, 300041 Timi\u0219, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucian Petru","family":"Jiga","sequence":"additional","affiliation":[{"name":"Department of Plastic, Aesthetic, Reconstructive and Hand Surgery, Evangelisches Krankenhaus Oldenburg, Medical Campus, University of Oldenburg, 26122 Oldenburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandru","family":"Nistor","sequence":"additional","affiliation":[{"name":"Division of Microsurgery, Pius Branzeu Center for Laparoscopic Surgery and Microsurgery, Victor Babes University of Medicine and Pharmacy, Timisoara, 300041 Timi\u0219, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vlad","family":"Dornean","sequence":"additional","affiliation":[{"name":"Division of Microsurgery, Pius Branzeu Center for Laparoscopic Surgery and Microsurgery, Victor Babes University of Medicine and Pharmacy, Timisoara, 300041 Timi\u0219, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sorin","family":"Barac","sequence":"additional","affiliation":[{"name":"Division of Microsurgery, Pius Branzeu Center for Laparoscopic Surgery and Microsurgery, Victor Babes University of Medicine and Pharmacy, Timisoara, 300041 Timi\u0219, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gratian","family":"Miclaus","sequence":"additional","affiliation":[{"name":"Neuromed Diagnostic Imaging Centre, Timisoara, 300218 Timi\u0219, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mihai","family":"Ionac","sequence":"additional","affiliation":[{"name":"Division of Microsurgery, Pius Branzeu Center for Laparoscopic Surgery and Microsurgery, Victor Babes University of Medicine and Pharmacy, Timisoara, 300041 Timi\u0219, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5217-7655","authenticated-orcid":false,"given":"Teodora","family":"Hoinoiu","sequence":"additional","affiliation":[{"name":"Division of Clinical Practical Skills, Victor Babes University of Medicine and Pharmacy, Timisoara, 300041 Timi\u0219, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,2]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (WHO) (2007). 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