{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T18:26:40Z","timestamp":1784312800575,"version":"3.55.0"},"reference-count":46,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T00:00:00Z","timestamp":1704412800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Learning Health Hospital","award":["LH.INV.F2019002"],"award-info":[{"award-number":["LH.INV.F2019002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diseases"],"abstract":"<jats:p>Acute pancreatitis (AP) is an increasingly frequent disease in which inflammation plays a crucial role. Fifty patients hospitalized with AP were included and peripheral blood samples were analyzed for B and T cell subpopulations at the time of hospitalization and 48 h after diagnosis. The Bedside Index of Severity in Acute Pancreatitis (BISAP) and length of hospital stay were also recorded. A healthy control (HC) group of 15 outpatients was included. AP patients showed higher neutrophil\/lymphocyte (N\/L) ratios and higher percentages of B cells than the HC group. The total B cell percentages were higher in patients with moderate\/severe AP than in patients with mild AP. The percentages of B cells as well as the percentages of the CD27\u2212IgD\u2212 B cell subset decreased from admission to 48 h after admission. The patients with higher BISAP scores showed lower percentages of peripheral lymphocytes but higher percentages of CD27\u2212IgD\u2212 B cells. Higher BISAP scores, N\/L ratios, and peripheral blood B cell levels emerged as predictors of hospital stay length in AP patients. Our findings underscore the importance of early markers for disease severity. Additionally, the N\/L ratio along with the BISAP score and circulating B cell levels form a robust predictive model for hospital stay duration of AP patients.<\/jats:p>","DOI":"10.3390\/diseases12010018","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T05:12:17Z","timestamp":1704431537000},"page":"18","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["B Cells and Double-Negative B Cells (CD27\u2212IgD\u2212) Are Related to Acute Pancreatitis Severity"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0664-2576","authenticated-orcid":false,"given":"Filipa","family":"Malheiro","sequence":"first","affiliation":[{"name":"Internal Medicine Department, LUZ SA\u00daDE, Hospital da Luz Lisboa, 1500-650 Lisboa, Portugal"},{"name":"CHRC, NOVA Medical School|Faculdade de Ci\u00eancias M\u00e9dicas (NMS|FCM), Universidade Nova de Lisboa, 1099-085 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9933-4075","authenticated-orcid":false,"given":"Miguel","family":"\u00c2ngelo-Dias","sequence":"additional","affiliation":[{"name":"CHRC, NOVA Medical School|Faculdade de Ci\u00eancias M\u00e9dicas (NMS|FCM), Universidade Nova de Lisboa, 1099-085 Lisboa, Portugal"},{"name":"Immunology Department, NOVA Medical School|Faculdade de Ci\u00eancias M\u00e9dicas (NMS|FCM), Universidade Nova de Lisboa, 1099-085 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Lopes","sequence":"additional","affiliation":[{"name":"CHRC, NOVA Medical School|Faculdade de Ci\u00eancias M\u00e9dicas (NMS|FCM), Universidade Nova de Lisboa, 1099-085 Lisboa, Portugal"},{"name":"Immunology Department, NOVA Medical School|Faculdade de Ci\u00eancias M\u00e9dicas (NMS|FCM), Universidade Nova de Lisboa, 1099-085 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4655-4987","authenticated-orcid":false,"given":"Sofia","family":"Azeredo-Lopes","sequence":"additional","affiliation":[{"name":"CHRC, NOVA Medical School|Faculdade de Ci\u00eancias M\u00e9dicas (NMS|FCM), Universidade Nova de Lisboa, 1099-085 Lisboa, Portugal"},{"name":"Department of Statistics and Operational Research, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catarina","family":"Martins","sequence":"additional","affiliation":[{"name":"CHRC, NOVA Medical School|Faculdade de Ci\u00eancias M\u00e9dicas (NMS|FCM), Universidade Nova de Lisboa, 1099-085 Lisboa, Portugal"},{"name":"Immunology Department, NOVA Medical School|Faculdade de Ci\u00eancias M\u00e9dicas (NMS|FCM), Universidade Nova de Lisboa, 1099-085 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4708-438X","authenticated-orcid":false,"given":"Luis Miguel","family":"Borrego","sequence":"additional","affiliation":[{"name":"CHRC, NOVA Medical School|Faculdade de Ci\u00eancias M\u00e9dicas (NMS|FCM), Universidade Nova de Lisboa, 1099-085 Lisboa, Portugal"},{"name":"Immunology Department, NOVA Medical School|Faculdade de Ci\u00eancias M\u00e9dicas (NMS|FCM), Universidade Nova de Lisboa, 1099-085 Lisboa, Portugal"},{"name":"Immunoallergy Department, LUZ SA\u00daDE, Hospital da Luz Lisboa, 1500-650 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1136\/gut.2008.152702","article-title":"The early prediction of mortality in acute pancreatitis: A large population-based study","volume":"57","author":"Wu","year":"2008","journal-title":"Gut"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1001\/jama.2020.20317","article-title":"Acute Pancreatitis: A Review","volume":"325","author":"Mederos","year":"2021","journal-title":"JAMA-J. 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