{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:07:21Z","timestamp":1760058441279,"version":"build-2065373602"},"reference-count":33,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T00:00:00Z","timestamp":1744243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japan Agency for Medical Research and Development (AMED)","award":["JP21ek0109524","JP24ek0109702","JPMJFR200N","JP20K08859","JP23K08005","71004191"],"award-info":[{"award-number":["JP21ek0109524","JP24ek0109702","JPMJFR200N","JP20K08859","JP23K08005","71004191"]}]},{"name":"Japan Science and Technology Agency (JST) FOREST Program","award":["JP21ek0109524","JP24ek0109702","JPMJFR200N","JP20K08859","JP23K08005","71004191"],"award-info":[{"award-number":["JP21ek0109524","JP24ek0109702","JPMJFR200N","JP20K08859","JP23K08005","71004191"]}]},{"name":"Ministry of Education, Culture, Sports, Science and Technology of Japan Grant-in-Aid for Scientific Research (C)","award":["JP21ek0109524","JP24ek0109702","JPMJFR200N","JP20K08859","JP23K08005","71004191"],"award-info":[{"award-number":["JP21ek0109524","JP24ek0109702","JPMJFR200N","JP20K08859","JP23K08005","71004191"]}]},{"name":"Nagoya University Hospital Funding for Clinical Research","award":["JP21ek0109524","JP24ek0109702","JPMJFR200N","JP20K08859","JP23K08005","71004191"],"award-info":[{"award-number":["JP21ek0109524","JP24ek0109702","JPMJFR200N","JP20K08859","JP23K08005","71004191"]}]},{"name":"Sumitomo Pharma Co., Ltd.","award":["JP21ek0109524","JP24ek0109702","JPMJFR200N","JP20K08859","JP23K08005","71004191"],"award-info":[{"award-number":["JP21ek0109524","JP24ek0109702","JPMJFR200N","JP20K08859","JP23K08005","71004191"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diseases"],"abstract":"<jats:p>Background\/Objectives: Our objective is to develop hormone-producing pituitary cells that can function in the same manner as the human body and provide more effective treatments than current hormone replacement therapy. We have already established a technique for generating hypothalamic\u2013pituitary organoids using feeder-free human pluripotent stem cells (hPSCs) and demonstrated their effectiveness in vivo through transplantation into hypopituitary mouse models. To prospectively determine the upper limit of transplanting adenohypophyseal cells into humans, we investigated the human maximum secretion capacity of adrenocorticotropic hormone (ACTH) and growth hormone (GH). Methods: We analyzed data from 28 patients with pituitary adenomas, among whom 16 evinced no abnormality of ACTH secretion and 12 showed no GH secretion on corticotropin-releasing hormone (CRH) and growth hormone-releasing hormone-2 (GHRP-2) stimulation testing. Results: The average ACTH peak value after CRH stimulation tests was 97.2 pg\/mL, and the average GH peak value after GHRP-2 stimulation tests was 25.1 ng\/mL. Conclusions: These data will likely serve as benchmarks of ACTH and GH secretion when transplanting cultured cells into humans.<\/jats:p>","DOI":"10.3390\/diseases13040112","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T08:46:20Z","timestamp":1744274780000},"page":"112","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Benchmark for Setting ACTH Cell Dosage in Clinical Regenerative Medicine for Post-Operative Hypopituitarism"],"prefix":"10.3390","volume":"13","author":[{"given":"Tatsuma","family":"Kondo","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Graduate School of Medicine, Nagoya University, Nagoya 468-0066, Japan"},{"name":"Department of Endocrinology and Diabetes, Graduate School of Medicine, Nagoya University, Nagoya 468-0066, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1924-7639","authenticated-orcid":false,"given":"Hidetaka","family":"Suga","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Diabetes, Graduate School of Medicine, Nagoya University, Nagoya 468-0066, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2820-5369","authenticated-orcid":false,"given":"Kazuhito","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Graduate School of Medicine, Nagoya University, Nagoya 468-0066, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9402-8079","authenticated-orcid":false,"given":"Yutaro","family":"Fuse","sequence":"additional","affiliation":[{"name":"Department of Artificial Intelligence Medicine, Graduate School of Medicine, Chiba University, Chiba 260-8722, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiki","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Graduate School of Medicine, Nagoya University, Nagoya 468-0066, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiaki","family":"Hirose","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Graduate School of Medicine, Nagoya University, Nagoya 468-0066, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harada","family":"Hideyuki","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Graduate School of Medicine, Nagoya University, Nagoya 468-0066, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuichi","family":"Nagata","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Graduate School of Medicine, Nagoya University, Nagoya 468-0066, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2484-1360","authenticated-orcid":false,"given":"Ryuta","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Graduate School of Medicine, Nagoya University, Nagoya 468-0066, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mce.2004.07.018","article-title":"Pituitary cell lines and their endocrine applications","volume":"228","author":"Ooi","year":"2004","journal-title":"Mol. 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