{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T02:02:23Z","timestamp":1784340143227,"version":"3.55.0"},"reference-count":52,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Secretar\u00eda de Salud de M\u00e9xico","award":["E022"],"award-info":[{"award-number":["E022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diseases"],"abstract":"<jats:p>Background\/Objectives: In adults with B-cell acute lymphoblastic leukemia (B-ALL), central nervous system (CNS) involvement represents a significant clinical challenge due to its association with adverse outcomes. Infiltration of blast cells into the CNS is primarily detected via cerebrospinal fluid (CSF) microscopy, the current gold standard diagnostic method, although it has limitations in terms of sensitivity. Quantitative polymerase chain reaction (qPCR) offers higher sensitivity and can support the diagnosis of CNS infiltration. This study assessed the mRNA expression levels of CD79A and IL7R in CSF to evaluate their potential for detecting CNS involvement in adults with B-ALL. Methods: CSF samples were collected from adults with B-ALL. The classification criteria for CNS Leukemia (CNS status) were used to evaluate CNS involvement. RNA was extracted from the CSF, and quantitative reverse transcription PCR (RT-qPCR) was used to measure the CD79A and IL7R mRNA expression levels. Results: A total of 19 treatment-na\u00efve adult patients with B-ALL were enrolled over a 19-month period. Four (21%) patients had CNS3 status. Four (21%) patients had CNS3 status. The results also showed that the expression levels of CD79A and IL7R mRNA were significantly higher (median fold change = 0.62 and 2.12, p &lt; 0.05, respectively) in the group with CNS3. Furthermore, using the Haldane-Anscombe correction and Fisher\u2019s exact test, we demonstrated an association between IL7R and CNS3 expression (odds ratio = \u221e, due to zero CNS+ in the IL7R group, p &lt; 0.05). Conclusions: CD79A and IL7R mRNA levels in CSF could be potential biomarkers for detecting CNS involvement in adult patients with B-ALL.<\/jats:p>","DOI":"10.3390\/diseases13070206","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T09:29:01Z","timestamp":1751362141000},"page":"206","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["CD79A and IL7R mRNA Levels in the Cerebrospinal Fluid of Adults with Acute B-Cell Lymphoblastic Leukemia: A Pilot Study"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5022-9505","authenticated-orcid":false,"given":"Andrea Iracema","family":"Mil\u00e1n Salvatierra","sequence":"first","affiliation":[{"name":"Hematology Service, Hospital Ju\u00e1rez de M\u00e9xico, Mexico City 07760, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3585-0051","authenticated-orcid":false,"given":"Juan Carlos","family":"Bravata Alc\u00e1ntara","sequence":"additional","affiliation":[{"name":"Genetics and Molecular Diagnostics Laboratory, Hospital Ju\u00e1rez de M\u00e9xico, Mexico City 07760, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edctor Manuel","family":"Alvarado Castro","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n de Enfermedades Tropicales, Universidad Aut\u00f3noma de Guerrero, Acapulco 39630, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2949-4924","authenticated-orcid":false,"given":"Estibeyesbo Said","family":"Plascencia Nieto","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faustino","family":"Cruz Leyto","sequence":"additional","affiliation":[{"name":"Hematology Service, Hospital Ju\u00e1rez de M\u00e9xico, Mexico City 07760, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica","family":"Tejeda Romero","sequence":"additional","affiliation":[{"name":"Hematology Service, Hospital Ju\u00e1rez de M\u00e9xico, Mexico City 07760, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Cruz Rico","sequence":"additional","affiliation":[{"name":"Hematology Service, Hospital Ju\u00e1rez de M\u00e9xico, Mexico City 07760, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bogar","family":"Pineda Terreros","sequence":"additional","affiliation":[{"name":"Hematology Service, Hospital Ju\u00e1rez de M\u00e9xico, Mexico City 07760, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"L\u00f3pez Palafox","sequence":"additional","affiliation":[{"name":"Hematology Service, Hospital Ju\u00e1rez de M\u00e9xico, Mexico City 07760, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Research Division, Hospital Ju\u00e1rez de M\u00e9xico, Mexico City 07760, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Ram\u00f3n","family":"Padilla Mendoza","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Reprogramming, Department of Physiology, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Bonilla Delgado","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Oncology, Hospital Regional de Alta Especialidad de Ixtapaluca, Servicios de Salud del Instituto Mexicano del Seguro Social para el Bienestar (IMSS-BINESTAR), Ixtapaluca 56530, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5816-0694","authenticated-orcid":false,"given":"Catalina","family":"Flores-Maldonado","sequence":"additional","affiliation":[{"name":"Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the Instituto Polit\u00e9cnico Nacional (Cinvestav-IPN), Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6240-3468","authenticated-orcid":false,"given":"Enoc Mariano","family":"Cort\u00e9s Malag\u00f3n","sequence":"additional","affiliation":[{"name":"Research Division, Hospital Ju\u00e1rez de M\u00e9xico, Mexico City 07760, Mexico"},{"name":"Genetics Laboratory, Hospital Nacional Homeop\u00e1tico, Mexico City 06800, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,1]]},"reference":[{"key":"ref_1","unstructured":"Puckett, Y., and Chan, O. (2024). Acute Lymphocytic Leukemia, StatPearls Publishing."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Neaga, A., Jimbu, L., Mesaros, O., Bota, M., Lazar, D., Cainap, S., Blag, C., and Zdrenghea, M. (2021). Why Do Children with Acute Lymphoblastic Leukemia Fare Better Than Adults?. Cancers, 13.","DOI":"10.3390\/cancers13153886"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1200\/JCO.2016.69.0073","article-title":"High Frequency and Poor Outcome of Philadelphia Chromosome-Like Acute Lymphoblastic Leukemia in Adults","volume":"35","author":"Roberts","year":"2017","journal-title":"J. Clin. Oncol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1182\/asheducation-2018.1.137","article-title":"Genetics and prognosis of ALL in children vs. adults","volume":"2018","author":"Roberts","year":"2018","journal-title":"Hematol. Am. Soc. Hematol. Educ. Program."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/s10555-020-09848-z","article-title":"Involvement of the central nervous system in acute lymphoblastic leukemia: Opinions on molecular mechanisms and clinical implications based on recent data","volume":"39","author":"Lenk","year":"2020","journal-title":"Cancer Metastasis Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1182\/blood.2023023568","article-title":"Management of ALL in adults: 2024 ELN recommendations from a European expert panel","volume":"143","author":"Gokbuget","year":"2024","journal-title":"Blood"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/s41586-018-0342-5","article-title":"Leukaemia hijacks a neural mechanism to invade the central nervous system","volume":"560","author":"Yao","year":"2018","journal-title":"Nature"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"346","DOI":"10.3324\/haematol.2016.147744","article-title":"The role of ZAP70 kinase in acute lymphoblastic leukemia infiltration into the central nervous system","volume":"102","author":"Alsadeq","year":"2017","journal-title":"Haematologica"},{"key":"ref_9","first-page":"521","article-title":"Identification and characterization of a murine model of BCR-ABL1+ acute B-lymphoblastic leukemia with central nervous system metastasis","volume":"42","author":"Yu","year":"2019","journal-title":"Oncol. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.1038\/leu.2009.42","article-title":"Increased risk for CNS relapse in pre-B cell leukemia with the t(1;19)\/TCF3-PBX1","volume":"23","author":"Jeha","year":"2009","journal-title":"Leukemia"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2735","DOI":"10.1182\/blood.2023021088","article-title":"The IL-7R antagonist lusvertikimab reduces leukemic burden in xenograft ALL via antibody-dependent cellular phagocytosis","volume":"143","author":"Lenk","year":"2024","journal-title":"Blood"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/j.it.2020.05.003","article-title":"It Takes Three Receptors to Raise a B Cell","volume":"41","author":"McLean","year":"2020","journal-title":"Trends Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.cyto.2009.12.001","article-title":"IL-7 receptor is expressed on adult pre-B-cell acute lymphoblastic leukemia and other B-cell derived neoplasms and correlates with expression of proliferation and survival markers","volume":"50","author":"Sasson","year":"2010","journal-title":"Cytokine"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1038\/leu.2016.9","article-title":"Pre-BCR signaling in precursor B-cell acute lymphoblastic leukemia regulates PI3K\/AKT, FOXO1 and MYC, and can be targeted by SYK inhibition","volume":"30","author":"Kohrer","year":"2016","journal-title":"Leukemia"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7268","DOI":"10.1038\/s41467-021-27197-5","article-title":"Interleukin-7 receptor alpha mutational activation can initiate precursor B-cell acute lymphoblastic leukemia","volume":"12","author":"Almeida","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1038\/s41375-021-01326-x","article-title":"Activated interleukin-7 receptor signaling drives B-cell acute lymphoblastic leukemia in mice","volume":"36","author":"Thomas","year":"2022","journal-title":"Leukemia"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1038\/s41467-022-28218-7","article-title":"An instructive role for Interleukin-7 receptor alpha in the development of human B-cell precursor leukemia","volume":"13","author":"Geron","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"ra116","DOI":"10.1126\/scisignal.aaf3949","article-title":"Dynamic pre-BCR homodimers fine-tune autonomous survival signals in B cell precursor acute lymphoblastic leukemia","volume":"9","author":"Erasmus","year":"2016","journal-title":"Sci. Signal"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Thastrup, M., Marquart, H.V., and Schmiegelow, K. (2022). Flow Cytometric Detection of Malignant Blasts in Cerebrospinal Fluid: A Biomarker of Central Nervous System Involvement in Childhood Acute Lymphoblastic Leukemia. Biomolecules, 12.","DOI":"10.3390\/biom12060813"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/ijlh.13869","article-title":"Technological features of blast identification in the cerebrospinal fluid: A systematic review of flow cytometry and laboratory haematology methods","volume":"44","author":"Frater","year":"2022","journal-title":"Int. J. Lab. Hematol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1007\/s11912-012-0248-6","article-title":"Diagnosis of acute leukemia in cerebrospinal fluid (CSF-acute leukemia)","volume":"14","author":"Higuera","year":"2012","journal-title":"Curr. Oncol. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1038\/s41375-020-01029-9","article-title":"Flowcytometric evaluation of cerebrospinal fluid in childhood ALL identifies CNS involvement better then conventional cytomorphology","volume":"35","author":"Pieters","year":"2021","journal-title":"Leukemia"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e2014075","DOI":"10.4084\/mjhid.2014.075","article-title":"Central nervous system involvement in adult acute lymphoblastic leukemia: Diagnostic tools, prophylaxis, and therapy","volume":"6","author":"Maurillo","year":"2014","journal-title":"Mediterr. J. Hematol. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1317577","DOI":"10.1080\/20013078.2017.1317577","article-title":"Analysis of extracellular RNA in cerebrospinal fluid","volume":"6","author":"Saugstad","year":"2017","journal-title":"J. Extracell. Vesicles"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1038\/s41698-021-00228-6","article-title":"Comprehensive RNA analysis of CSF reveals a role for CEACAM6 in lung cancer leptomeningeal metastases","volume":"5","author":"Li","year":"2021","journal-title":"NPJ Precis. Oncol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S1525-1578(10)60018-9","article-title":"Detection of central nervous system leukemia in children with acute lymphoblastic leukemia by real-time polymerase chain reaction","volume":"7","author":"Pine","year":"2005","journal-title":"J. Mol. Diagn."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Peterffy, B., Nadasi, T.J., Krizsan, S., Horvath, A., Mark, A., Barna, G., Timar, B., Almasi, L., Muller, J., and Csanadi, K. (2024). Digital PCR-based quantification of miR-181a in the cerebrospinal fluid aids patient stratification in pediatric acute lymphoblastic leukemia. Sci. Rep., 14.","DOI":"10.1038\/s41598-024-79733-0"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2751","DOI":"10.1038\/s41375-022-01714-x","article-title":"Central nervous system involvement in childhood acute lymphoblastic leukemia: Challenges and solutions","volume":"36","author":"Thastrup","year":"2022","journal-title":"Leukemia"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.1182\/blood-2018-04-844209","article-title":"IL7R is associated with CNS infiltration and relapse in pediatric B-cell precursor acute lymphoblastic leukemia","volume":"132","author":"Alsadeq","year":"2018","journal-title":"Blood"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Lenk, L., Carlet, M., Vogiatzi, F., Spory, L., Winterberg, D., Cousins, A., Vossen-Gajcy, M., Ibruli, O., Vokuhl, C., and Cario, G. (2021). CD79a promotes CNS-infiltration and leukemia engraftment in pediatric B-cell precursor acute lymphoblastic leukemia. Commun. Biol., 4.","DOI":"10.1038\/s42003-020-01591-z"},{"key":"ref_31","unstructured":"Stanley Maloy, K.H. (2013). FAB Classification of Leukemia. Brenner\u2019s Encyclopedia of Genetics, Academic Press. [2nd ed.]."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1038\/leu.2012.120","article-title":"EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes","volume":"26","author":"Lhermitte","year":"2012","journal-title":"Leukemia"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/978-1-4939-8935-5_11","article-title":"Biobanking of Cerebrospinal Fluid","volume":"1897","author":"Tashjian","year":"2019","journal-title":"Methods Mol. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1002\/pbc.24981","article-title":"Clinical features and early treatment response of central nervous system involvement in childhood acute lymphoblastic leukemia","volume":"61","author":"Levinsen","year":"2014","journal-title":"Pediatr. Blood Cancer"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1261\/rna.036863.112","article-title":"Identification of extracellular miRNA in human cerebrospinal fluid by next-generation sequencing","volume":"19","author":"Burgos","year":"2013","journal-title":"RNA"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.htct.2021.09.016","article-title":"Comparative analysis between cytomorphology and flow cytometry methods in central nervous system infiltration assessment in oncohematological patients","volume":"45","author":"Rocha","year":"2023","journal-title":"Hematol. Transfus. Cell Ther."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1212\/01.wnl.0000261909.28915.83","article-title":"CSF flow cytometry greatly improves diagnostic accuracy in CNS hematologic malignancies","volume":"68","author":"Bromberg","year":"2007","journal-title":"Neurology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e70452","DOI":"10.1002\/cam4.70452","article-title":"Cerebrospinal Fluid Flow Cytometry in Pediatric Acute Lymphoblastic Leukemia: A Multicenter Retrospective Study in China","volume":"13","author":"Tu","year":"2024","journal-title":"Cancer Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1200\/JCO.2008.17.7089","article-title":"Identification of leptomeningeal disease in aggressive B-cell non-Hodgkin\u2019s lymphoma: Improved sensitivity of flow cytometry","volume":"27","author":"Quijano","year":"2009","journal-title":"J. Clin. Oncol."},{"key":"ref_41","first-page":"271","article-title":"Flow cytometric characterization of cerebrospinal fluid cells","volume":"80","author":"Kraan","year":"2011","journal-title":"Cytom. B Clin. Cytom."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1046\/j.1365-2141.2001.02505.x","article-title":"Flow cytometry and the study of central nervous disease in patients with acute leukaemia","volume":"112","author":"Subira","year":"2001","journal-title":"Br. J. Haematol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1309\/AJCPANA7ER1ABMZI","article-title":"Flow cytometric immunophenotyping of cerebrospinal fluid specimens","volume":"135","author":"Craig","year":"2011","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1182\/blood.2023022020","article-title":"Entirely noninvasive outcome prediction in central nervous system lymphomas using circulating tumor DNA","volume":"143","author":"Heger","year":"2024","journal-title":"Blood"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1186\/s12967-020-02415-8","article-title":"MicroRNA-181a as novel liquid biopsy marker of central nervous system involvement in pediatric acute lymphoblastic leukemia","volume":"18","author":"Egyed","year":"2020","journal-title":"J. Transl. Med."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1080\/10428194.2018.1499906","article-title":"IL7R overexpression in adult acute lymphoblastic leukemia is associated to JAK\/STAT pathway mutations and identifies patients who could benefit from targeted therapies","volume":"60","author":"Gianfelici","year":"2019","journal-title":"Leuk. Lymphoma"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Wang, C., Kong, L., Kim, S., Lee, S., Oh, S., Jo, S., Jang, I., and Kim, T.D. (2022). The Role of IL-7 and IL-7R in Cancer Pathophysiology and Immunotherapy. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231810412"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1038\/s41577-024-01073-x","article-title":"Towards a unifying model for B-cell receptor triggering","volume":"25","author":"Degn","year":"2025","journal-title":"Nat. Rev. Immunol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"4997","DOI":"10.1182\/bloodadvances.2023011145","article-title":"A brain organoid\/ALL coculture model reveals the AP-1 pathway as critically associated with CNS involvement of BCP-ALL","volume":"8","author":"Gebing","year":"2024","journal-title":"Blood Adv."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1183\/09031936.00136408","article-title":"Small studies: Strengths and limitations","volume":"32","author":"Hackshaw","year":"2008","journal-title":"Eur. Respir. J."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"6843","DOI":"10.1038\/s41467-021-27232-5","article-title":"Single-cell analysis identifies dynamic gene expression networks that govern B cell development and transformation","volume":"12","author":"Lee","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_52","first-page":"43","article-title":"Central memory CD4+ T cells dominate the normal cerebrospinal fluid","volume":"80","author":"Smitt","year":"2011","journal-title":"Cytom. B Clin. Cytom."}],"container-title":["Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-9721\/13\/7\/206\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:02:30Z","timestamp":1760032950000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-9721\/13\/7\/206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,1]]},"references-count":52,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["diseases13070206"],"URL":"https:\/\/doi.org\/10.3390\/diseases13070206","relation":{},"ISSN":["2079-9721"],"issn-type":[{"value":"2079-9721","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,1]]}}}