{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:15:46Z","timestamp":1791994546787,"version":"4.4.0"},"reference-count":51,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diseases"],"abstract":"<jats:p>Purpose: To assess the association between hair loss in females and various biomarkers including hemoglobin, iron, ferritin, zinc, selenium, calcium, vitamin D, vitamin B12, folic acid, and thyroid hormones. Patients and methods: This study enrolled 100 women presenting with hair loss and 100 age-matched healthy controls. Venous blood samples were collected for analysis of hematological, hormonal and biochemical parameters. Results: The mean age of participants was comparable between groups (43.06 \u00b1 10.76 vs. 41.39 \u00b1 7.94 years; p = 0.88). Hair loss in females had significantly lower mean levels of Hb (11.45 \u00b1 0.39 vs. 13.09 \u00b1 0.46 g\/dL; p &lt; 0.001), iron (70.14 \u00b1 7.85 vs. 94.42 \u00b1 5.61 \u00b5g\/dL; p &lt; 0.001) and ferritin (39.34 \u00b1 3.71 vs. 48.09 \u00b1 5.31 ng\/mL), all with p &lt; 0.001. Serum levels of selenium (67.11 \u00b1 5.53 vs. 71.45 \u00b1 4.05 \u00b5g\/L), zinc (86.07 \u00b1 3.98 vs. 88.87 \u00b1 2.03 \u00b5g\/L), copper (90.71 \u00b1 3.48 vs. 104.84 \u00b1 5.38 \u00b5g\/L), and calcium (8.61 \u00b1 0.28 vs. 9.11 \u00b1 0.27 mg\/dL) were significantly reduced in women with hair loss (p &lt; 0.001). Thyroid hormones were also significantly lower in the hair loss group, including TSH (1.74 \u00b1 0.25 vs. 2.35 \u00b1 0.39 \u00b5IU\/mL) and FREE T4 (1.11 \u00b1 0.11 vs. 1.32 \u00b1 0.12 ng\/dL), despite remaining within the normal reference ranges. Patients also showed lower serum folate (6.17 \u00b1 0.63 vs. 6.96 \u00b1 0.41 ng\/mL), vitamin B12 (185.52 \u00b1 35.27 vs. 258.30 \u00b1 52.84 pg\/mL), and vitamin D (26.32 \u00b1 2.98 vs. 32.20 \u00b1 3.76 ng\/dL) levels (p &lt; 0.001). Conclusions: Hair loss in females is significantly associated with reduced levels of circulating hemoglobin, iron, copper, selenium, vitamin D, vitamin B12, folate, thyroid-stimulating hormone and FREE T4 hormone.<\/jats:p>","DOI":"10.3390\/diseases13110352","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T09:16:24Z","timestamp":1761729384000},"page":"352","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Quantitative Analysis of Selected Circulating Hematological Biomarkers, Essential Minerals, Vitamins, and Thyroid Hormones in Females Affected by Hair Loss"],"prefix":"10.3390","volume":"13","author":[{"given":"Saad","family":"Al-Fawaeir","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Science, Faculty of Allied Medical Sciences, Jadara University, Irbid 21110, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim","family":"Al-Odat","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science, Faculty of Allied Medical Sciences, Jadara University, Irbid 21110, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1111\/exd.14073","article-title":"A \u201chair-raising\u201d history of alopecia areata","volume":"29","author":"Broadley","year":"2020","journal-title":"Exp. 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