{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T09:26:40Z","timestamp":1787045200173,"version":"build-2736575974"},"reference-count":94,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diseases"],"abstract":"<jats:p>Background: Red blood cell (RBC) antibody screening in blood donors is a key component of transfusion safety. However, reported prevalence and antibody specificities vary widely across studies, and a consolidated global estimate remains limited. This study aimed to quantify the prevalence of RBC antibody detection among blood donors, identify factors associated with variation in reported prevalence, and summarize antibody specificities. Methods: This systematic review and meta-analysis was conducted in accordance with PRISMA 2020 guidelines. PubMed, MEDLINE, Embase, Scopus, and Google Scholar were searched from inception to 27 August 2025 for observational studies reporting RBC antibody detection in blood donors. Methodological quality was assessed using the JBI Critical Appraisal Checklist for Studies Reporting Prevalence Data. Pooled prevalence estimates were synthesized using random-effects models. Subgroup analyses, meta-regression, leave-one-out sensitivity analyses, and publication-bias assessments were performed. Associations between ABO blood group and RBC antibody detection were summarized using pooled odds ratios. The review protocol was registered in PROSPERO (CRD420251181987). Results: Forty-one studies comprising 43 reports and 3,561,088 blood donors from 18 countries were included. The pooled prevalence of RBC antibody detection was 0.2% (95% CI: 0.2\u20130.3%), with substantial between-study heterogeneity (I2: 99.61%) and a 95% prediction interval of 0.0\u20131.4%. Prevalence was higher in female donors than in male donors (0.5% vs. 0.1%). Publication year was inversely associated with reported prevalence, although temporal and methodological effects could not be separated. An exploratory analysis based on six studies showed higher estimated odds of antibody detection among blood group AB donors (OR: 1.20, 95% CI: 1.07\u20131.34). Among clinically significant antibodies, Rh and Kell specificities were predominated. Anti-Mia was prominent in Southeast Asian populations. Conclusions: RBC antibody detection among blood donors was uncommon overall but varied considerably across settings. Interpretation is limited by substantial methodological heterogeneity, uneven geographic representation, and incomplete reporting of donor characteristics. The pooled estimate should be interpreted as an international benchmark rather than a uniform prevalence applicable to individual blood services. This large-scale synthesis may inform context-specific donor antibody screening, confirmatory testing, component management, and hemovigilance policies.<\/jats:p>","DOI":"10.3390\/diseases14080298","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T08:08:41Z","timestamp":1787040521000},"page":"298","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Global Prevalence and Specificity of Red Blood Cell Antibodies in Blood Donors: A Systematic Review and Meta-Analysis"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7404-6664","authenticated-orcid":false,"given":"Thitinat","family":"Duangchan","sequence":"first","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Hematology and Transfusion Science Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8246-1710","authenticated-orcid":false,"given":"Moltira","family":"Promkan","sequence":"additional","affiliation":[{"name":"Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Nakhon Pathom 73170, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1062-4007","authenticated-orcid":false,"given":"Susan D.","family":"Kraner","sequence":"additional","affiliation":[{"name":"Sanders-Brown Center on Aging, University of Kentucky College of Medicine, Lexington, KY 40536, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4432-2699","authenticated-orcid":false,"given":"Nurdina","family":"Charong","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Hematology and Transfusion Science Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"770","DOI":"10.3390\/epidemiologia5040052","article-title":"Voluntary Blood Donation in Modern Healthcare: Trends, Challenges, and Opportunities","volume":"5","author":"Krsek","year":"2024","journal-title":"Epidemiologia"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1182\/blood-2018-08-833962","article-title":"Transfusion-related red blood cell alloantibodies: Induction and consequences","volume":"133","author":"Tormey","year":"2019","journal-title":"Blood"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"102634","DOI":"10.1016\/j.coi.2025.102634","article-title":"Red blood cell alloimmunization immunogenetic risk factor","volume":"96","author":"Pedini","year":"2025","journal-title":"Curr. Opin. Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"95","DOI":"10.21307\/immunohematology-2020-021","article-title":"Clinical significance of antibodies to antigens in the ABO, MNS, P1PK, Rh, Lutheran, Kell, Lewis, Duffy, Kidd, Diego, Yt, and Xg blood group systems","volume":"35","author":"Thornton","year":"2019","journal-title":"Immunohematology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1111\/j.1537-2995.2006.00708.x","article-title":"Red blood cell alloantibodies after transfusion: Factors influencing incidence and specificity","volume":"46","author":"Schonewille","year":"2006","journal-title":"Transfusion"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"96","DOI":"10.4103\/0973-6247.137442","article-title":"Clinical significance of antibody specificities to M, N and Lewis blood group system","volume":"8","author":"Makroo","year":"2014","journal-title":"Asian J. Transfus. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"95","DOI":"10.21307\/immunohematology-2019-240","article-title":"MNS blood group system: A review","volume":"25","author":"Reid","year":"2009","journal-title":"Immunohematology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1046\/j.1423-0410.2002.00212.x","article-title":"Immunological characterization of anti-Mia, a red blood cell alloantibody, in Taiwan","volume":"83","author":"Lo","year":"2002","journal-title":"Vox Sang."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"93","DOI":"10.21307\/immunohematology-2020-047","article-title":"Routine indirect antiglobulin testing of blood donors\u2014A further step toward blood safety: An experience from a tertiary care center in northern India","volume":"36","author":"Malhotra","year":"2020","journal-title":"Immunohematology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.transci.2014.03.004","article-title":"Prevalence of irregular red blood cell antibodies among healthy blood donors in Delhi population","volume":"50","author":"Garg","year":"2014","journal-title":"Transfus. Apher. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"280","DOI":"10.4103\/ijmr.IJMR_296_19","article-title":"Prevalence of red blood cell antibodies in whole blood donors: A single-centre experience in north India","volume":"152","author":"Solanki","year":"2020","journal-title":"Indian J. Med. Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Rastogi, V., Sidhar, M., Rajesh, H., Kaushal, N., Bhardwaj, S., Lochan, D., Malik, V., and Jha, R. (2025). Exploring trends of red blood cell alloimmunization among transfusion recipients and healthy blood donors at a tertiary care hospital centre: A comprehensive analysis. J. Lab. Physicians.","DOI":"10.25259\/JLP_344_2024"},{"key":"ref_13","first-page":"e86347","article-title":"Prevalence and Specificity of Red Blood Cell Alloantibodies in Patients in a Tertiary Care Center in Meghalaya, India","volume":"17","author":"Devi","year":"2025","journal-title":"Cureus"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"42","DOI":"10.4103\/0973-6247.75997","article-title":"Prevalence and specificities of red cell alloantibodies among blood recipients in the Malaysian state of Kelantan","volume":"5","author":"Ali","year":"2011","journal-title":"Asian J. Transfus. Sci."},{"key":"ref_15","first-page":"204","article-title":"Unexpected red cell antibodies in donors and patients at Siriraj Hospital","volume":"10","author":"Bejrachandra","year":"1979","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1111\/vox.13537","article-title":"Prevalence of red blood cell alloantibodies among blood donors in the French Military Blood Institute: A 10-year retrospective study","volume":"118","author":"Pons","year":"2023","journal-title":"Vox Sang."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Aldurayhim, M., Aldosari, S., Sajid, M.R., Aljatham, A., Binjomah, A., Alsughayir, A., Alfalah, Y., Aloumi, A., Hussaini, M., and Adeeb, S. (2026). Frequency of alloimmunization in patients on regular blood transfusion in Riyadh, Saudi Arabia: A multicenter retrospective study. J. Clin. Med., 15.","DOI":"10.3390\/jcm15062340"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1097\/XEB.0000000000000054","article-title":"Methodological guidance for systematic reviews of observational epidemiological studies reporting prevalence and cumulative incidence data","volume":"13","author":"Munn","year":"2015","journal-title":"Int. J. Evid.-Based Healthc."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Romyasamit, C., Turni, C., Anusuriya, S., and Duangchan, T. (Glob. Health J., 2026). Antimicrobial resistance patterns of Helicobacter pylori in Thailand: A systematic review and meta-analysis, Glob. Health J., in press.","DOI":"10.1016\/j.glohj.2026.06.008"},{"key":"ref_20","unstructured":"Higgins, J.P.T., Thomas, J., Chandler, J., Cumpston, M., Li, T., Page, M.J., and Welch, V.A. (2024). Chapter 15: Interpreting results and drawing conclusions. Cochrane Handbook for Systematic Reviews of Interventions, Version 6.5, Cochrane. Available online: https:\/\/training.cochrane.org\/handbook."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4913","DOI":"10.21608\/ejhm.2018.10173","article-title":"Red Blood Cell Alloantibodies in Healthy Egyptian Blood Donors","volume":"72","author":"Hashem","year":"2018","journal-title":"Egypt. J. Hosp. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"131","DOI":"10.21307\/immunohematology-2021-020","article-title":"Immune erythrocyte antibodies in adult patients with sickle cell disease and blood donors in Lagos, Nigeria: A comparative study","volume":"37","author":"Adewoyin","year":"2021","journal-title":"Immunohematology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"421","DOI":"10.36348\/sjpm.2022.v07i11.005","article-title":"Prevalence and Specificities of Immune Red Cell Antibodies in Adult Patients with Sickle Cell Anaemia and Blood Donors in Uyo, South-South Nigeria: A Case-Control Study","volume":"7","author":"Akpan","year":"2022","journal-title":"Saudi J. Pathol. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1159\/000085740","article-title":"Frequency of red blood cell alloantibody in Kuwaiti population","volume":"14","author":"Ameen","year":"2005","journal-title":"Med. Princ. Pract."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5","DOI":"10.21307\/immunohematology-2021-002","article-title":"Group O blood donors in Iran: Evaluation of isoagglutinin titers and immunoglobulin G subclasses","volume":"37","author":"Arabi","year":"2021","journal-title":"Immunohematology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2207","DOI":"10.13005\/bbra\/2385","article-title":"Prevalence of Red Blood Cell Alloantibodies in Blood Donors of Zanjan Province; the Preliminary Report of the North West of Iran","volume":"13","author":"Babaei","year":"2016","journal-title":"Biosci. Biotechnol. Res. Asia"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"227","DOI":"10.4103\/GJTM.GJTM_28_19","article-title":"Frequency of Irregular Red Cell Antibodies in Blood Donor Population","volume":"4","author":"Bharathan","year":"2019","journal-title":"Glob. J. Transfus. Med."},{"key":"ref_28","first-page":"828","article-title":"Study on application value of antiglobulin test of 72,684 blood donors","volume":"33","author":"Chen","year":"2020","journal-title":"J. Clin. Hematol."},{"key":"ref_29","first-page":"597","article-title":"Application of detecting irregular antibodies of blood donors by microplate papain method and anti-human globulin microcolumn gel technique","volume":"36","author":"Dong","year":"2023","journal-title":"J. Clin. Hematol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/S0338-4535(78)80080-4","article-title":"Irregular antibody screening by Groupamatic at the Paris (C.N.T.S.) and Toulouse (C.R.T.S.) blood transfusion centers","volume":"21","author":"Ducos","year":"1978","journal-title":"Rev. Fr. Transfus. Immunohematol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"26","DOI":"10.25100\/cm.v30i.1.118","article-title":"Embarazo y transfusion y su asociaci\u00f3n con aloanticuerpos inesperados de significancia cl\u00ednica contra ant\u00edgenos eritrocitarios","volume":"30","author":"Rovetto","year":"1999","journal-title":"Colomb. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/tme.12511","article-title":"RBC alloantibody prevalence and specificity in a Western Norwegian tertiary hospital","volume":"29","author":"Erikstein","year":"2019","journal-title":"Transfus. Med."},{"key":"ref_33","first-page":"33","article-title":"A comparison of two automated methods for the detection and identification of red blood cell alloantibodies","volume":"5","author":"Garozzo","year":"2007","journal-title":"Blood Transfus."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"246","DOI":"10.35841\/1836-1935.12.9.246-250","article-title":"An evaluation of irregular antibody profile in voluntary blood donors and blood transfusion recipients","volume":"12","author":"Gupta","year":"2019","journal-title":"Australas. Med. J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1111\/trf.15004","article-title":"Prevalence and risk factors for RBC alloantibodies in blood donors in the Recipient Epidemiology and Donor Evaluation Study-III (REDS-III)","volume":"59","author":"Karafin","year":"2019","journal-title":"Transfusion"},{"key":"ref_36","first-page":"EC12","article-title":"Erythrocyte Alloimmunization and Autoimmunization among Blood Donors and Recipients visiting a Tertiary Care Hospital","volume":"11","author":"Kaur","year":"2017","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_37","first-page":"194","article-title":"Red cell alloantibodies in Lao blood donors","volume":"45","author":"Keokhamphoui","year":"2014","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"47","DOI":"10.4103\/gjtm.gjtm_1_22","article-title":"Antibody Screening and Identification in Voluntary Blood Donors\u2014Need of the Hour","volume":"7","author":"Luhar","year":"2022","journal-title":"Glob. J. Transfus. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"17","DOI":"10.4103\/ajts.AJTS_4_17","article-title":"Prevalence of irregular red cell antibody in healthy blood donors attending a tertiary care hospital in North India","volume":"12","author":"Makroo","year":"2018","journal-title":"Asian J. Transfus. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1111\/j.1537-2995.1965.tb01184.x","article-title":"Incidence of irregular antibodies occurring in healthy donor sera","volume":"5","author":"Myhre","year":"1965","journal-title":"Transfusion"},{"key":"ref_41","first-page":"C1","article-title":"Prevalence of Red Cell AlloAntibodies & AutoAntibodies in Patient & Donor attending a Tertiary Care Hospital in South Gujarat","volume":"8","author":"Nathani","year":"2021","journal-title":"Int. J. Contemp. Med. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"8871102","DOI":"10.1155\/ah\/8871102","article-title":"Assessment of Unexpected (Non-ABO) Red Blood Cell Antibodies and Their Associated Clinical Conditions Among Patients and Blood Donors Attending University Teaching Hospital of Kigali (CHUK) and Rwanda Blood Transfusion Division","volume":"2025","author":"Niyibizi","year":"2025","journal-title":"Adv. Hematol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1179\/1607845412Y.0000000020","article-title":"Screening of blood donors for erythrocyte alloantibodies","volume":"17","author":"Pahuja","year":"2012","journal-title":"Hematology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"5","DOI":"10.32553\/ijmbs.v6i5.2533","article-title":"Prevalence of Irregular Red Cell Antibodies in Healthy Blood Donors at a Stand-Alone Blood Centre in Gujarat","volume":"6","author":"Patel","year":"2022","journal-title":"Int. J. Med. Biomed. Stud."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1093\/milmed\/164.10.705","article-title":"The incidence of unexpected antibodies in blood products collected at a military donor center","volume":"164","author":"Plumbley","year":"1999","journal-title":"Mil. Med."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"16","DOI":"10.4103\/0973-6247.106718","article-title":"Frequencies and specificities of red cell alloantibodies in the Southern Thai population","volume":"7","author":"Promwong","year":"2013","journal-title":"Asian J. Transfus. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"293","DOI":"10.2298\/VSP231105022R","article-title":"Prevalence and identification of irregular erythrocyte antibodies in voluntary blood donors in the territory of Southeast Serbia","volume":"81","author":"Randjelovic","year":"2024","journal-title":"Vojnosanit. Pregl."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"219","DOI":"10.4103\/GJTM.GJTM_22_19","article-title":"Prevalence of Irregular Red Blood Cell Antibodies Among Healthy Blood Donors in South India","volume":"4","author":"Sachan","year":"2019","journal-title":"Glob. J. Transfus. Med."},{"key":"ref_49","first-page":"221","article-title":"Evaluation of a solid-phase test for erythrocyte antibody screening of pregnant women, patients and blood donors","volume":"71","author":"Sallander","year":"1996","journal-title":"Vox Sang."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"103963","DOI":"10.1016\/j.transci.2024.103963","article-title":"Irregular red blood cell antibodies, abnormal hemoglobin and dangerous universal blood donor insights from a public blood center in a Brazilian metropolitan area","volume":"63","author":"Santos","year":"2024","journal-title":"Transfus. Apher. Sci."},{"key":"ref_51","first-page":"10","article-title":"Prevalence and specificity of red cell alloantibodies in regular blood donors in a tertiary hospital in the east coast of Malaysia","volume":"24","author":"Shafini","year":"2017","journal-title":"Int. Med. J."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.transci.2013.08.008","article-title":"Incidence of clinically significant antibodies in patients and healthy blood donors: A prospective cross-sectional study from a tertiary healthcare center in India","volume":"50","author":"Tiwari","year":"2014","journal-title":"Transfus. Apher. Sci."},{"key":"ref_53","first-page":"a929","article-title":"Blood donor characterization with positive irregular antibodies in Monter\u00eda, Colombia, 2012\u20132015","volume":"35","author":"Toledo","year":"2019","journal-title":"Rev. Cuba. Hematol. Inmunol. Hemoter."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"73","DOI":"10.4103\/ajts.AJTS_118_19","article-title":"Prevalence of irregular red cell antibody in transfusion recipients vis-a-vis healthy blood donors attending a tertiary care hospital in North India","volume":"16","author":"Yadav","year":"2022","journal-title":"Asian J. Transfus. Sci."},{"key":"ref_55","first-page":"590","article-title":"Application of antibody type and titer in blood products and donor retention","volume":"37","author":"Yang","year":"2024","journal-title":"J. Clin. Hematol."},{"key":"ref_56","first-page":"630","article-title":"Screening analysis of irregular antibodies from random donor population in Shaoguan area","volume":"15","author":"Zhu","year":"2007","journal-title":"Zhongguo Shi Yan Xue Ye Xue Za Zhi"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1111\/vox.13296","article-title":"Red blood cell alloimmunization among recipients of blood transfusion in India: A systematic review and meta-analysis","volume":"117","author":"Shastry","year":"2022","journal-title":"Vox Sang."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"111","DOI":"10.4103\/ajts.AJTS_39_20","article-title":"Prevalence and specificity of red blood cell alloantibodies and autoantibodies in transfused Iranian \u03b2-thalassemia patients: A systematic review and meta-analysis","volume":"16","author":"Rostamian","year":"2022","journal-title":"Asian J. Transfus. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"417","DOI":"10.4314\/ahs.v24i3.46","article-title":"Frequency of red blood cell allo-immunization in transfused patients with sickle cell disease in Africa: A systematic review with meta-analysis","volume":"24","author":"Nwagha","year":"2024","journal-title":"Afr. Health Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"106","DOI":"10.14740\/jocmr6142","article-title":"Prevalence of Alloimmunization Events in Thalassemia Patients With Repeated Transfusions in the Rhesus Blood Group System: A Systematic Review and Meta Analysis","volume":"17","author":"Indriani","year":"2025","journal-title":"J. Clin. Med. Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"445","DOI":"10.2147\/JBM.S548152","article-title":"Erythrocyte Alloimmunization and Transfusion Strategies in Sickle Cell Disease: A Single-Center Analysis","volume":"16","author":"Yusuf","year":"2025","journal-title":"J. Blood Med."},{"key":"ref_62","first-page":"e60158","article-title":"Red Blood Cell Alloimmunization in Pregnancy: A Review of the Pathophysiology, Prevalence, and Risk Factors","volume":"16","author":"Adani","year":"2024","journal-title":"Cureus"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"95","DOI":"10.4103\/0974-2727.199627","article-title":"Comparison of micro column technology with conventional tube methods for antibody detection","volume":"9","author":"Garg","year":"2017","journal-title":"J. Lab. Physicians"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.17219\/acem\/140317","article-title":"High-sensitivity detection of clinically significant red blood cell antibodies by the column agglutination technique","volume":"30","author":"Charong","year":"2021","journal-title":"Adv. Clin. Exp. Med."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1111\/j.1365-3148.1993.tb00061.x","article-title":"Sensitivity of column agglutination technology in detecting unexpected red cell antibodies","volume":"3","author":"Pinkerton","year":"1993","journal-title":"Transfus. Med."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"140","DOI":"10.4103\/0973-6247.98914","article-title":"Automation in immunohematology","volume":"6","author":"Bajpai","year":"2012","journal-title":"Asian J. Transfus. Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1007\/s12288-019-01127-4","article-title":"Performance Evaluation of Automated Immunohematology Analyzer IH-500 for Blood Bank Testing","volume":"35","author":"Park","year":"2019","journal-title":"Indian J. Hematol. Blood Transfus."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"268","DOI":"10.3343\/alm.2013.33.4.268","article-title":"Economic and workflow analysis of a blood bank automated system","volume":"33","author":"Shin","year":"2013","journal-title":"Ann. Lab. Med."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1111\/vox.13235","article-title":"Routine donor red cell antibody screening: Considering the alternate strategy","volume":"117","author":"Sivakaanthan","year":"2022","journal-title":"Vox Sang."},{"key":"ref_70","first-page":"43","article-title":"Prevalence of Rh, Kell, Kidd, Duffy, and MNS antigens in the Hispanic donor population of South Texas","volume":"38","author":"Wafford","year":"2022","journal-title":"Immunohematology"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s12288-012-0155-x","article-title":"Incidence of Red Cell Alloantibody among the Transfusion Recipients of Universiti Kebangsaan Malaysia Medical Centre","volume":"29","author":"Yousuf","year":"2013","journal-title":"Indian J. Hematol. Blood Transfus."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"4311","DOI":"10.1182\/bloodadvances.2023012241","article-title":"Maternal red blood cell alloimmunization prevalence in the United States","volume":"8","author":"Sugrue","year":"2024","journal-title":"Blood Adv."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"S128","DOI":"10.1016\/j.htct.2024.04.128","article-title":"Assessment of erythrocyte alloimmunization among patients treated at a Brazilian university hospital","volume":"46","author":"Contelli","year":"2024","journal-title":"Hematol. Transfus. Cell Ther."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1893","DOI":"10.1097\/MS9.0000000000003092","article-title":"Investigation of alloimmunization in beta-thalassemia major patients: A cross-sectional study","volume":"87","author":"Arianezhad","year":"2025","journal-title":"Ann. Med. Surg."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"101625","DOI":"10.1016\/j.cegh.2024.101625","article-title":"Distribution of ABO, Rh, and MNS blood groups from students in Walailak University, Thailand: A descriptive cross-sectional study","volume":"28","author":"Duangchan","year":"2024","journal-title":"Clin. Epidemiol. Glob. Health"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"e0107","DOI":"10.55374\/jseamed.v6i0.107","article-title":"Frequencies of predicted Mia antigen among Southern Thai blood donors","volume":"6","author":"Khosidworachet","year":"2022","journal-title":"J. Southeast Asian Med. Res."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"103378","DOI":"10.1016\/j.transci.2022.103378","article-title":"Study on the antigens and antibodies of Mur and Mia blood groups in southern China","volume":"61","author":"Li","year":"2022","journal-title":"Transfus. Apher. Sci."},{"key":"ref_78","first-page":"65","article-title":"Anti-Mia and Anti-E: The Most Common Clinically Significant Red Cell Alloantibodies in Patients at Phramongkutklao Hospital","volume":"70","author":"Srijinda","year":"2017","journal-title":"RTA Med. J."},{"key":"ref_79","first-page":"1337","article-title":"The Prevalence of Red Blood Cell Alloantibodies in Lower Northern Thailand","volume":"99","author":"Sasjeenpong","year":"2016","journal-title":"J. Med. Assoc. Thai."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"621","DOI":"10.3329\/bjms.v24i2.81537","article-title":"Prevalence of Mia antigen and anti-Mia antibody among patients from a university hospital in Malaysia","volume":"24","author":"Navaretnam","year":"2025","journal-title":"Bangladesh J. Med. Sci."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"537","DOI":"10.2147\/JBM.S558414","article-title":"Red Cell Alloimmunization Among Transfused Chronic Kidney Disease Patients: A Hospital-Based Study in Southwestern Uganda","volume":"16","author":"John","year":"2025","journal-title":"J. Blood Med."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"e177","DOI":"10.5001\/omj.2020.95","article-title":"Prevalence of red blood cell alloimmunization among transfused chronic kidney disease patients in Hospital Universiti Sains Malaysia","volume":"35","author":"Yusoff","year":"2020","journal-title":"Oman Med. J."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/s41423-023-01074-1","article-title":"Immune regulatory networks coordinated by glycans and glycan-binding proteins in autoimmunity and infection","volume":"20","author":"Pinho","year":"2023","journal-title":"Cell. Mol. Immunol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1038\/345229a0","article-title":"Molecular genetic basis of the histo-blood group ABO system","volume":"345","author":"Yamamoto","year":"1990","journal-title":"Nature"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Par\u00e9, G., Chasman, D.I., Kellogg, M., Zee, R.Y., Rifai, N., Badola, S., Miletich, J.P., and Ridker, P.M. (2008). Novel association of ABO histo-blood group antigen with soluble ICAM-1: Results of a genome-wide association study of 6578 women. PLoS Genet., 4.","DOI":"10.1371\/journal.pgen.1000118"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.1111\/trf.12486","article-title":"Comparison of antibody titers in donor specimens and associated AS-1 leukoreduced donor units","volume":"54","author":"Hill","year":"2014","journal-title":"Transfusion"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"11","DOI":"10.21307\/immunohematology-2018-004","article-title":"A review of in vitro methods to predict the clinical significance of red blood cell alloantibodies","volume":"33","author":"Nance","year":"2018","journal-title":"Immunohematology"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1093\/ajcp\/109.6.769","article-title":"Problems associated with passively transfused blood group alloantibodies","volume":"109","author":"Garratty","year":"1998","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"120","DOI":"10.21307\/immunohematology-2019-591","article-title":"Large-scale use of red blood cell units containing alloantibodies","volume":"16","author":"Combs","year":"2000","journal-title":"Immunohematology"},{"key":"ref_90","unstructured":"Joint United Kingdom Blood Transfusion and Tissue Transplantation Services Professional Advisory Committee (JPAC) (2013). Guidelines for the Blood Transfusion Services in the United Kingdom, JPAC. Available online: https:\/\/www.transfusionguidelines.org\/red-book\/chapter-12-donation-testing-red-cell-immunohaematology."},{"key":"ref_91","unstructured":"Canadian Blood Services (2026, August 07). Serological Best Practices: Whole Blood Donors with Antibodies. Available online: https:\/\/professionaleducation.blood.ca\/en\/transfusion\/best-practices\/serological-best-practices."},{"key":"ref_92","unstructured":"European Directorate for the Quality of Medicines & HealthCare (EDQM) (2025). Guide to the Preparation, Use and Quality Assurance of Blood Components, Council of Europe. [22nd ed.]. Available online: https:\/\/www.edqm.eu\/en\/blood-guide."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"2094","DOI":"10.1007\/s00268-020-05461-x","article-title":"Barriers to Effective Transfusion Practices in Limited-Resource Settings: From Infrastructure to Cultural Beliefs","volume":"44","author":"Mohammed","year":"2020","journal-title":"World J. Surg."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/S0895-4356(99)00204-8","article-title":"Misleading funnel plot for detection of bias in meta-analysis","volume":"53","author":"Tang","year":"2000","journal-title":"J. Clin. Epidemiol."}],"container-title":["Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-9721\/14\/8\/298\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T08:36:33Z","timestamp":1787042193000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-9721\/14\/8\/298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,18]]},"references-count":94,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["diseases14080298"],"URL":"https:\/\/doi.org\/10.3390\/diseases14080298","relation":{},"ISSN":["2079-9721"],"issn-type":[{"value":"2079-9721","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,18]]}}}