{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T22:06:31Z","timestamp":1761948391516,"version":"build-2065373602"},"reference-count":19,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T00:00:00Z","timestamp":1524528000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diseases"],"abstract":"<jats:p>In this study, the use of fibrin rich in leukocytes and platelets (L-PRF) was explored to heal osteomyelitis ulcers in a diabetic foot. The goal was to standardize the utilization of L-PRF in patients with osteomyelitis to direct it for healing. L-PRF was obtained autologously from the peripheral blood of the diabetic patients (n = 3) having osteomyelitis and skin lesions for at least six months. The L-PRF and supernatant serum were inserted into the skin lesion to the bone after a surgical debridement. The evolution of lesions over time was analyzed. All three patients showed positivity to the Probe-to-Bone test and Nuclear Magnetic Resonance detected cortico-periosteal thickening and\/or outbreaks of spongy cortical osteolysis in adjacency of the ulcer. The infections were caused by Cocci Gram-positive bacteria, such as S. Aureus, S. \u03b2-hemolytic, S. Viridans and Bacilli; and Gram-negative such as Pseudomonas, Proteus, Enterobacter; and yeast, Candida. The blood count did not show any significant alterations. To date, all three patients have healed skin lesions (in a patient for about two years) with no evidence of infection. These preliminary results showed that L-PRF membranes could be a new method of therapy in such problematic diseases. Overall, the L-PRF treatment in osteomyelitis of a diabetic foot seems to be easy and cost-effective by regenerative therapy of chronic skin lesions. In addition, it will improve our understanding of wound healing.<\/jats:p>","DOI":"10.3390\/diseases6020030","type":"journal-article","created":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T04:44:48Z","timestamp":1524545088000},"page":"30","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Use of Leukocyte Platelet (L-PRF) Rich Fibrin in Diabetic Foot Ulcer with Osteomyelitis (Three Clinical Cases Report)"],"prefix":"10.3390","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2098-6690","authenticated-orcid":false,"given":"Alessandro","family":"Crisci","sequence":"first","affiliation":[{"name":"Unit of Dermosurgery Cutaneous Transplantations and Hard-to-Heal Wound, \u201cVilla Fiorita\u201d Private Hospital, 81031 Aversa, CE, Italy"},{"name":"School of Medicine, University of Salerno Italy, 84084 Fisciano, SA, Italy"},{"name":"Institute for the Studies and Care of Diabetics, Abetaia, 81020 Casagiove, CE, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Marotta","sequence":"additional","affiliation":[{"name":"Institute for the Studies and Care of Diabetics, Abetaia, 81020 Casagiove, CE, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Licito","sequence":"additional","affiliation":[{"name":"Institute for the Studies and Care of Diabetics, Abetaia, 81020 Casagiove, CE, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edda","family":"Serra","sequence":"additional","affiliation":[{"name":"Institute for the Studies and Care of Diabetics, Abetaia, 81020 Casagiove, CE, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9060-2915","authenticated-orcid":false,"given":"Giulio","family":"Benincasa","sequence":"additional","affiliation":[{"name":"Pathological Anatomy, \u201cPineta Grande\u201d Private Hospital, 81030 Castelvolturno, CE, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela","family":"Crisci","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Surgery, Vasile Goldis Western University of Arad, 310025 Arad, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/S0140-6736(04)16727-5","article-title":"Osteomyelitis","volume":"364","author":"Lew","year":"2004","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1055\/s-0029-1214158","article-title":"Osteomyelitis of the Long Bones","volume":"23","author":"Calhoun","year":"2009","journal-title":"Semin. 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Available online: http:\/\/scholarscompass.vcu.edu\/etd\/3749."}],"container-title":["Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-9721\/6\/2\/30\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:01:52Z","timestamp":1760194912000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-9721\/6\/2\/30"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,24]]},"references-count":19,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,6]]}},"alternative-id":["diseases6020030"],"URL":"https:\/\/doi.org\/10.3390\/diseases6020030","relation":{},"ISSN":["2079-9721"],"issn-type":[{"type":"electronic","value":"2079-9721"}],"subject":[],"published":{"date-parts":[[2018,4,24]]}}}