{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:44:28Z","timestamp":1792291468067,"version":"4.4.0"},"reference-count":34,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dentistry Journal"],"abstract":"<jats:p>Objectives: Combining mechanical plaque control, the physical removal of oral biofilm, with chemical plaque control, the use of agents to inhibit biofilm formation, is effective in preventing periodontal disease. Chlorogenic acid (CGA) found in coffee beans has medicinal effects, such as anti-inflammatory and antibacterial properties. Periodontal pathogens are difficult to reach in certain areas with traditional self-care tools, such as toothbrushes. Additionally, the viscous biofilm is difficult to remove using mechanical plaque control alone. Therefore, this study aimed to evaluate the efficacy of CGA in chemical plaque control. Methods: The mRNA and protein expression of inflammatory cytokines in lipopolysaccharide (LPS)-stimulated human gingival fibroblasts (HGFs) and human periodontal ligament fibroblasts (HPDLs) in the presence of CGA were analyzed using reverse transcription-qPCR and enzyme-linked immunosorbent assay. Additionally, the proliferation levels of oral bacteria in the presence of CGA were evaluated. Results: CGA suppressed mRNA and protein expression levels of the inflammatory cytokines, interleukin (IL)-1\u03b2 and IL-8, in HGFs and HPDLs stimulated with Porphyromonas gingivalis LPS. Furthermore, CGA inhibited bacterial proliferation of Streptococcus mutans, Aggregatibacter actinomycetemcomitans, P. gingivalis, and Fusobacterium nucleatum. Conclusions: This study demonstrated that CGA exhibits anti-inflammatory effects on gingiva and periodontal ligaments, and antibacterial effects against oral bacteria. These results indicate the potential application of CGA in chemical plaque control and suggest its use in preventing periodontal disease progression.<\/jats:p>","DOI":"10.3390\/dj14020125","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T08:58:21Z","timestamp":1771837101000},"page":"125","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anti-Inflammatory Effects on Periodontal Tissue and Antibacterial Effects on Oral Bacteria of Chlorogenic Acid"],"prefix":"10.3390","volume":"14","author":[{"given":"Yuya","family":"Suzuki","sequence":"first","affiliation":[{"name":"Field of Advanced Conservative Dentistry and Periodontology, Periodontology, Course of Clinical Science, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4592-6061","authenticated-orcid":false,"given":"Kosuke","family":"Maruyama","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0757-5184","authenticated-orcid":false,"given":"Masato","family":"Mikami","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soh","family":"Sato","sequence":"additional","affiliation":[{"name":"Field of Advanced Conservative Dentistry and Periodontology, Periodontology, Course of Clinical Science, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan"},{"name":"Department of Periodontology, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata 951-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/s41431-018-0265-5","article-title":"Meta-analysis of genome-wide association studies of aggressive and chronic periodontitis identifies two novel risk loci","volume":"27","author":"Munz","year":"2019","journal-title":"Eur. J. Hum. Genet."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Wang, M., Deng, X., Chen, H., Diao, Y., Liu, C., Gao, J., Tang, X., Li, X., Liu, Y., and Duan, J. (2023). Frailty mediated the association between tooth loss and mortality in the oldest old individuals: A cohort study. Front. Public Health, 11.","DOI":"10.3389\/fpubh.2023.1285226"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Li, T., Wang, X., Liu, Z., Zhang, Z., Zhang, Y., Wang, Z., Feng, Y., Wang, Q., Guo, X., and Tang, X. (2023). Prevalence and prognostic significance of malnutrition in patients with abnormal glycemic status and coronary artery disease: A multicenter cohort study in China. Nutrients, 15.","DOI":"10.3390\/nu15030732"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1111\/jcpe.13557","article-title":"Associations of periodontal disease and tooth loss with all-cause and cause-specific mortality in the Sister Study","volume":"48","author":"Wu","year":"2021","journal-title":"J. Clin. Periodontol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"S5","DOI":"10.1111\/jcpe.12682","article-title":"Role of microbial biofilms in the maintenance of oral health and in the development of dental caries and periodontal diseases. Consensus report of group 1 of the Joint EFP\/ORCA workshop on the boundaries between caries and periodontal disease","volume":"44","author":"Sanz","year":"2017","journal-title":"J. Clin. Periodontol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1016\/j.jmb.2019.05.016","article-title":"The structure of dental plaque microbial communities in the transition from health to dental caries and periodontal disease","volume":"431","author":"Valm","year":"2019","journal-title":"J. Mol. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"20147","DOI":"10.1074\/jbc.M115.655787","article-title":"Porphyromonas gingivalis stimulates bone resorption by enhancing RANKL (receptor activator of NF-\u03baB ligand) through activation of Toll-like receptor 2 in osteoblasts","volume":"290","author":"Kassem","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1006\/bbrc.2001.5842","article-title":"Heterogeneous expression levels of Toll-like receptor 4 and downregulation of Toll-like receptor 4 expression on human gingival fibroblasts by Porphyromonas gingivalis lipopolysaccharide","volume":"288","author":"Wang","year":"2001","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Steemers, E., Talbi, W.M.I., Hogervorst, J.M.A., Schoenmaker, T., and de Vries, T.J. (2025). IL-1 receptor antagonist anakinra inhibits the effect of IL-1\u03b2- mediated osteoclast formation by periodontal ligament fibroblasts. Biology, 14.","DOI":"10.3390\/biology14030250"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"188","DOI":"10.4103\/0970-9290.116675","article-title":"Evaluation and comparison of interleukin-8 (IL-8) level in gingival crevicular fluid in health and severity of periodontal disease: A clinico-biochemical study","volume":"24","author":"Lagdive","year":"2013","journal-title":"Indian J. Dent. Res."},{"key":"ref_11","first-page":"S77","article-title":"Efficacy of homecare regimens for mechanical plaque removal in managing gingivitis a meta review","volume":"42","author":"Slot","year":"2015","journal-title":"J. Clin. Periodontol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1111\/j.1601-5037.2008.00330.x","article-title":"The efficacy of interdental brushes on plaque and parameters of periodontal inflammation: A systematic review","volume":"6","author":"Slot","year":"2008","journal-title":"Int. J. Dent. Hyg."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Marsh, P.D. (2006). Dental plaque as a biofilm and a microbial community\u2014Implications for health and disease. BMC Oral Health, 6.","DOI":"10.1186\/1472-6831-6-S1-S14"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1111\/j.1600-051X.2012.01883.x","article-title":"Effect of a chlorhexidine mouthrinse on plaque, gingival inflammation and staining in gingivitis patients: A systematic review","volume":"39","author":"Slot","year":"2012","journal-title":"J. Clin. Periodontol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1002\/(SICI)1097-0010(20000515)80:7<1033::AID-JSFA595>3.0.CO;2-T","article-title":"Chlorogenic acids and other cinnamates: Nature, occurrence, dietary burden, absorption and metabolism","volume":"80","author":"Clifford","year":"2000","journal-title":"J. Sci. Food Agric."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Rojas-Gonz\u00e1lez, A., Figueroa-Hern\u00e1ndez, C.Y., Gonz\u00e1lez-Rios, O., Su\u00e1rez-Quiroz, M.L., Gonz\u00e1lez-Amaro, R.M., Hern\u00e1ndez-Estrada, Z.J., and Rayas-Duarte, P. (2022). Coffee Chlorogenic Acids Incorporation for Bioactivity Enhancement of Foods: A Review. Molecules, 27.","DOI":"10.3390\/molecules27113400"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.biopha.2017.10.064","article-title":"Chlorogenic acid (CGA): A pharmacological review and call for further research","volume":"97","author":"Naveed","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2127642","DOI":"10.1155\/2022\/2127642","article-title":"Chlorogenic acid as a positive regulator in LPS-PG-induced inflammation via TLR4\/MyD88-mediated NF-\u03baB and PI3K\/MAPK signaling cascades in human gingival fibroblasts","volume":"2022","author":"Park","year":"2022","journal-title":"Mediat. Inflamm."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1007\/s10266-022-00753-1","article-title":"Effects of nicotine and lipopolysaccharide stimulation on adhesion molecules in human gingival endothelial cells","volume":"111","author":"Sato","year":"2023","journal-title":"Odontology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1007\/s10266-024-00988-0","article-title":"Anti-inflammatory effects of hesperidin on human gingival fibroblasts stimulated by lipopolysaccharide of Porphyromonas gingivalis in vitro","volume":"113","author":"Suzuki","year":"2025","journal-title":"Odontology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1002\/mlf2.12147","article-title":"Measurement of optical density of microbes by multi-light path transmission method","volume":"4","author":"Wang","year":"2024","journal-title":"mLife"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1006\/bbrc.2000.3060","article-title":"Toll-like receptor 4-mediated signal pathway induced by Porphyromonas gingivalis lipopolysaccharide in human gingival fibroblasts","volume":"273","author":"Wang","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1111\/j.1600-0765.2012.01500.x","article-title":"Comparison of CCL28, interleukin-8, interleukin-1\u03b2 and tumor necrosis factor-alpha in subjects with gingivitis, chronic periodontitis and generalized aggressive periodontitis","volume":"48","author":"Ertugrul","year":"2013","journal-title":"J. Periodontal. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/imr.12621","article-title":"Overview of the IL-1 family in innate inflammation and acquired immunity","volume":"281","author":"Dinarello","year":"2018","journal-title":"Immunol. Rev."},{"key":"ref_25","first-page":"cm1","article-title":"Interleukin-1 (IL-1) pathway","volume":"3","author":"Weber","year":"2010","journal-title":"Sci. Signal."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1111\/jre.12324","article-title":"Gingival crevicular fluid interleukin-8 and lipoxin A4 levels of smokers and nonsmokers with different periodontal status: A cross-sectional study","volume":"51","author":"Pamuk","year":"2016","journal-title":"J. Periodontal. Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Liang, N., and Kitts, D.D. (2018). Chlorogenic acid (CGA) isomers alleviate interleukin 8 (IL-8) production in caco-2 cells by decreasing phosphorylation of p38 and increasing cell integrity. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19123873"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/nri3785","article-title":"Periodontitis: From microbial immune subversion to systemic inflammation","volume":"15","author":"Hajishengallis","year":"2015","journal-title":"Nat. Rev. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1111\/omi.12065","article-title":"The inflammophilic character of the periodontitis-associated microbiota","volume":"29","author":"Hajishengallis","year":"2014","journal-title":"Mol. Oral Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4729","DOI":"10.1128\/IAI.66.10.4729-4732.1998","article-title":"Role of Fusobacterium nucleatum and coaggregation in anaerobe survival in planktonic and biofilm oral microbial communities during aeration","volume":"66","author":"Bradshaw","year":"1998","journal-title":"Infect. Immun."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1093\/oxfordjournals.jbchem.a022735","article-title":"Porphyromonas gingivalis proteinases as virulence determinants in progression of periodontal diseases","volume":"128","author":"Kadowaki","year":"2000","journal-title":"J. Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.foodchem.2007.05.091","article-title":"Chlorogenic acid and caffeine contents in various commercial brewed coffees","volume":"106","author":"Fujioka","year":"2008","journal-title":"Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e040754","DOI":"10.1136\/bmjopen-2020-040754","article-title":"Duration of gargling and rinsing among frequent mouthwash users: A cross-sectional study","volume":"10","author":"Phillips","year":"2020","journal-title":"BMJ Open"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1177\/00220345231151921","article-title":"The role of gingival fibroblasts in the pathogenesis of periodontitis","volume":"102","author":"Wielento","year":"2023","journal-title":"J. Dent. Res."}],"container-title":["Dentistry Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-6767\/14\/2\/125\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T09:23:31Z","timestamp":1771838611000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-6767\/14\/2\/125"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,22]]},"references-count":34,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["dj14020125"],"URL":"https:\/\/doi.org\/10.3390\/dj14020125","relation":{},"ISSN":["2304-6767"],"issn-type":[{"value":"2304-6767","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,22]]}}}