{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T05:19:36Z","timestamp":1791695976238,"version":"4.3.4"},"reference-count":61,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dentistry Journal"],"abstract":"<jats:p>Background\/Objectives: This study evaluated 3D palatal shape variation to distinguish population affinities, aiming to enhance forensic and anthropological assessments of unknown human remains. Methods: Maxillary dental casts (n = 100 per group) from four population groups, Australian Europeans, Malaysian Malays, Malaysian Chinese and Malaysian Indians, were digitised using a 3D scanner. Fourteen gingival and two mid-palatal landmarks and 99 surface points (semi-landmarks) were placed to capture the overall palatal shape. Shape data were aligned using Generalised Procrustes Analysis (GPA) followed by Principal Component Analysis (PCA) to explore shape variation. Canonical Variate Analysis (CVA) and Mahalanobis distances were used to assess group differences. Classification performance was evaluated using accuracy, sensitivity, specificity, precision, and F1 score. Results: The first five principal components (each explaining \u22655% of the observed variation) were retained for CVA. CV1 accounted for 88% of the between-group variance, showing clear separation of Europeans from the clustered Malay\u2013Chinese group, with Indians appearing intermediate. European palates were longer, narrower, deeper, and more anteriorly tapered. Significant shape differences were found between all groups, except between Malays and Chinese. The overall classification accuracy was 45%, with better performance for European and Indian groups. Specificity was higher across all groups (0.70\u20130.90), while sensitivity, precision, and F1 scores were moderate. Conclusions: Geometric morphometric analysis revealed population-level differences in palatal shape, with moderate accuracy and high specificity. These findings support its role as a complementary tool alongside other data in forensic and anthropological applications. While palatal shape alone cannot definitively classify populations, it may be useful for excluding certain groups.<\/jats:p>","DOI":"10.3390\/dj14050258","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:43:51Z","timestamp":1777448631000},"page":"258","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Three-Dimensional Variation of the Human Hard Palate Across Populations: A Geometric Morphometrics Study"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6546-9817","authenticated-orcid":false,"given":"Thao Liang","family":"Chiam","sequence":"first","affiliation":[{"name":"School of Dentistry, College of Health, Adelaide University, Adelaide, SA 5005, Australia"},{"name":"Faculty of Dentistry, The National University of Malaysia, Kuala Lumpur 50300, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9097-9130","authenticated-orcid":false,"given":"Angela","family":"Gurr","sequence":"additional","affiliation":[{"name":"School of Dentistry, College of Health, Adelaide University, Adelaide, SA 5005, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8668-7744","authenticated-orcid":false,"given":"Toby","family":"Hughes","sequence":"additional","affiliation":[{"name":"School of Dentistry, College of Health, Adelaide University, Adelaide, SA 5005, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyle","family":"Palmer","sequence":"additional","affiliation":[{"name":"School of Public Health, College of Health, Adelaide University, Adelaide, SA 5005, Australia"},{"name":"Australian Institute for Machine Learning, Adelaide University, Adelaide, SA 5005, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7780-243X","authenticated-orcid":false,"given":"Denice","family":"Higgins","sequence":"additional","affiliation":[{"name":"School of Dentistry, College of Health, Adelaide University, Adelaide, SA 5005, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"19785","DOI":"10.1038\/s41598-024-70985-4","article-title":"Genetic and environmental impact on variation in the palatal dimensions in permanent dentition: A twin study","volume":"14","author":"Papievis","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"cjae076","DOI":"10.1093\/ejo\/cjae076","article-title":"Relative contributions of genetic and environmental factors to palatal morphology: A longitudinal twin study","volume":"47","author":"Giri","year":"2024","journal-title":"Eur. J. Orthod."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1002\/ajpa.21006","article-title":"Understanding race and human variation: Why forensic anthropologists are good at identifying race","volume":"139","author":"Ousley","year":"2009","journal-title":"Am. J. Phys. Anthropol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Lu, C., Ahmed, R., Lamri, A., and Anand, S.S. (2022). Use of race, ethnicity, and ancestry data in health research. PLoS Glob. Public Health, 2.","DOI":"10.31219\/osf.io\/z4xty"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1101\/gr.2529604","article-title":"Evidence for gradients of human genetic diversity within and among continents","volume":"14","author":"Serre","year":"2004","journal-title":"Genome Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s40246-022-00380-5","article-title":"A framework for research into continental ancestry groups of the UK Biobank","volume":"16","author":"Constantinescu","year":"2022","journal-title":"Hum. Genom."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Rosenberg, N.A., Mahajan, S., Ramachandran, S., Zhao, C., Pritchard, J.K., and Feldman, M.W. (2005). Clines, Clusters, and the Effect of Study Design on the Inference of Human Population Structure. PLoS Genet., 1.","DOI":"10.1371\/journal.pgen.0010070"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2381","DOI":"10.1126\/science.1078311","article-title":"Genetic structure of human populations","volume":"298","author":"Rosenberg","year":"2002","journal-title":"Science"},{"key":"ref_9","first-page":"309","article-title":"What Are We Really Estimating in Forensic Anthropological Practice, Population Affinity or Ancestry?","volume":"4","author":"Spradley","year":"2022","journal-title":"Forensic Anthropol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"pgad217","DOI":"10.1093\/pnasnexus\/pgad217","article-title":"Inferring human neutral genetic variation from craniodental phenotypes","volume":"2","author":"Rathmann","year":"2023","journal-title":"PNAS Nexus"},{"key":"ref_11","unstructured":"Rhine, S. (1990). Non-metric skull racing. Skeletal Attribution of Race: Methods for Forensic Anthropology, University of New Mexico, Maxwell Museum of Anthropology."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1111\/1556-4029.14231","article-title":"Ancestry Estimation Using Cranial and Postcranial Macromorphoscopic Traits","volume":"65","author":"Spiros","year":"2020","journal-title":"J. Forensic Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1080\/20961790.2019.1697060","article-title":"Evaluation of ancestry from human skeletal remains: A concise review","volume":"5","author":"Cunha","year":"2020","journal-title":"Forensic Sci. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1111\/1556-4029.12812","article-title":"Palate Shape and Depth: A Shape-Matching and Machine Learning Method for Estimating Ancestry from Human Skeletal Remains","volume":"60","author":"Maier","year":"2015","journal-title":"J. Forensic Sci."},{"key":"ref_15","unstructured":"Reichs, K.J. (1998). Forensic Osteology: Advances in the Identification of Human Remains, Charles C Thomas. [2nd ed.]."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1111\/j.1556-4029.2009.01176.x","article-title":"Evidentiary Standards for Forensic Anthropology","volume":"54","author":"Christensen","year":"2009","journal-title":"J. Forensic Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1111\/j.1556-4029.2011.01875.x","article-title":"A Simulation for Exploring the Effects of the \u201cTrait List\u201d Method\u2019s Subjectivity on Consistency and Accuracy of Ancestry Estimations","volume":"56","author":"Hughes","year":"2011","journal-title":"J. Forensic Sci."},{"key":"ref_18","unstructured":"Hefner, J.T., Emanovsky, P., Byrd, J., and Ousley, S. (2007, January 19\u201324). The value of experience, education, and methods in ancestry prediction. Proceedings of the 59th Annual Meeting of the American Academy of Forensic Sciences, San Antonio, TX, USA."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Bookstein, F.L. (1997). Morphometric Tools for Landmark Data, Cambridge University Press.","DOI":"10.2307\/2534038"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1080\/11250000409356545","article-title":"Geometric morphometrics: Ten years of progress following the \u2018revolution\u2019","volume":"71","author":"Adams","year":"2004","journal-title":"Ital. J. Zool."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1111\/j.1558-5646.2008.00321.x","article-title":"The evolutionary role of modularity and integration in the hominoid cranium","volume":"62","author":"Mitteroecker","year":"2008","journal-title":"Evol. Int. J. Org. Evol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1002\/ajpa.10023","article-title":"Thin-plate spline analysis of allometry and sexual dimorphism in the human craniofacial complex","volume":"117","author":"Rosas","year":"2002","journal-title":"Am. J. Phys. Anthropol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1111\/j.1556-4029.2007.00627.x","article-title":"Sexual dimorphism in America: Geometric morphometric analysis of the craniofacial region","volume":"53","author":"Kimmerle","year":"2008","journal-title":"J. Forensic Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/s00427-016-0539-2","article-title":"Size, shape, and form: Concepts of allometry in geometric morphometrics","volume":"226","author":"Klingenberg","year":"2016","journal-title":"Dev. Genes. Evol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1002\/ar.20442","article-title":"Three-dimensional procrustes analysis of modern human craniofacial form","volume":"290","author":"Cabanis","year":"2007","journal-title":"Anat. Rec."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1111\/1556-4029.12910","article-title":"Quantification of Maxillary Dental Arcade Curvature and the Estimation of Biological Ancestry in Forensic Anthropology","volume":"61","author":"Clark","year":"2016","journal-title":"J. Forensic Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2883","DOI":"10.1097\/SCS.0000000000007796","article-title":"The Influence of Sex and Ancestry on Three-Dimensional Palate Shape","volume":"32","author":"Brandebura","year":"2021","journal-title":"J. Craniofac. Surg."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"106275","DOI":"10.1016\/j.archoralbio.2025.106275","article-title":"Palatal morphology: A systematic review of the association of palatal shape with genetic ancestry, sex and age","volume":"175","author":"Chiam","year":"2025","journal-title":"Arch. Oral Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"104562","DOI":"10.1016\/j.jdent.2023.104562","article-title":"Reproducibility of the digital palate in forensic investigations: A two-year retrospective cohort study on twins","volume":"135","author":"Mikolicz","year":"2023","journal-title":"J. Dent."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1111\/1556-4029.15110","article-title":"The discriminative potential of palatal geometric analysis for sex discrimination and human identification","volume":"67","author":"Simon","year":"2022","journal-title":"J. Forensic Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Simon, B., Lipt\u00e1k, L., Lipt\u00e1k, K., T\u00e1rnoki, \u00c1.D., T\u00e1rnoki, D.L., Melicher, D., and V\u00e1g, J. (2020). Application of intraoral scanner to identify monozygotic twins. BMC Oral Health, 20.","DOI":"10.1186\/s12903-020-01261-w"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Puleio, F., Lo Giudice, G., Alibrandi, A., Campione, I., Di Spirito, F., and Lo Giudice, R. (2025). Precision in Facial Measurements: Comparative Analysis Between a Digital 3D Scanner and an Analog Instrument. Dent. J., 13.","DOI":"10.3390\/dj13090395"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Townsend, G.C., Pinkerton, S.K., Rogers, J.R., Bockmann, M.R., and Hughes, T.E. (2015). Twin Studies: Research in Genes, Teeth and Faces, University of Adelaide Press.","DOI":"10.20851\/twin-studies"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"49","DOI":"10.26575\/daj.v19i2.120","article-title":"Variation in Dental Crown Morphology in Malaysian Populations","volume":"19","author":"Khamis","year":"2006","journal-title":"Dent. Anthropol. J."},{"key":"ref_35","unstructured":"Australian Bureau of Statistics (2025, September 22). Population: Cencus, Available online: https:\/\/www.abs.gov.au\/statistics\/people\/population\/population-census\/latest-release."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"(2002). Federation Dentaire Internationale (FDI) tooth-numbering system. Am. J. Orthod. Dentofac. Orthop., 121, 175.","DOI":"10.1016\/S0889-5406(02)70212-6"},{"key":"ref_37","unstructured":"(2025, May 14). 3D Slicer. Available online: https:\/\/www.slicer.org\/."},{"key":"ref_38","unstructured":"Jolesz, F.A. (2014). 3D Slicer: A Platform for Subject-Specific Image Analysis, Visualization, and Clinical Support. Intraoperative Imaging and Image-Guided Therapy, Springer."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/j.mri.2012.05.001","article-title":"3D Slicer as an image computing platform for the Quantitative Imaging Network","volume":"30","author":"Fedorov","year":"2012","journal-title":"Magn. Reson. Imaging"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.1111\/2041-210X.13669","article-title":"SlicerMorph: An open and extensible platform to retrieve, visualize and analyse 3D morphology","volume":"12","author":"Rolfe","year":"2021","journal-title":"Methods Ecol. Evol."},{"key":"ref_41","first-page":"103","article-title":"Semilandmarks: A method for quantifying curves and surfaces","volume":"24","author":"Gunz","year":"2013","journal-title":"Hystrix"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"obz016","DOI":"10.1093\/iob\/obz016","article-title":"A Practical Guide to Sliding and Surface Semilandmarks in Morphometric Analyses","volume":"1","author":"Bardua","year":"2019","journal-title":"Integr. Org. Biol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Watanabe, A. (2018). How many landmarks are enough to characterize shape and size variation?. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0198341"},{"key":"ref_44","unstructured":"Zheng, G., Li, S., and Sz\u00e9kely, G. (2017). Chapter 9\u2014Morpho and Rvcg\u2014Shape Analysis in R: R-Packages for Geometric Morphometrics, Shape Analysis and Surface Manipulations. Statistical Shape and Deformation Analysis, Academic Press."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"40","DOI":"10.2307\/2992207","article-title":"Extensions of the procrustes method for the optimal superimposition of landmarks","volume":"39","author":"Rohlf","year":"1990","journal-title":"Syst. Zool."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Dryden, I.L., and Mardia, K.V. (2016). Statistical Shape Analysis: With Applications in R, John Wiley & Sons.","DOI":"10.1002\/9781119072492"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Bookstein, F.L. (2014). Measuring and Reasoning: Numerical Inference in the Sciences, Cambridge University Press.","DOI":"10.1017\/CBO9781139161923"},{"key":"ref_48","unstructured":"Obertov\u00e1, Z., Stewart, A., and Cattaneo, C. (2020). Chapter 5.3\u2014Geometric morphometrics. Statistics and Probability in Forensic Anthropology, Academic Press."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1007\/s11692-011-9109-8","article-title":"Linear Discrimination, Ordination, and the Visualization of Selection Gradients in Modern Morphometrics","volume":"38","author":"Mitteroecker","year":"2011","journal-title":"Evol. Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1080\/10635150590947258","article-title":"Distances and Directions in Multidimensional Shape Spaces: Implications for Morphometric Applications","volume":"54","author":"Klingenberg","year":"2005","journal-title":"Syst. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1111\/j.1558-5646.1998.tb02018.x","article-title":"Geometric morphometrics of developmental instability: Analyzing patterns of fluctuating asymmetry with procrustes methods","volume":"52","author":"Klingenberg","year":"1998","journal-title":"Evol. Int. J. Org. Evol."},{"key":"ref_52","first-page":"7","article-title":"A field comes of age: Geometric morphometrics in the 21st century","volume":"24","author":"Adams","year":"2013","journal-title":"Hystrix Ital. J. Mammal."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1093\/ejo\/cjx014","article-title":"Morphometric covariation between palatal shape and skeletal pattern in Class II growing subjects","volume":"39","author":"Paoloni","year":"2017","journal-title":"Eur. J. Orthod."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Paoloni, V., Gastaldi, G., Franchi, L., De Razza, F.C., and Cozza, P. (2020). Evaluation of the morphometric covariation between palatal and craniofacial skeletal morphology in class III malocclusion growing subjects. BMC Oral Health, 20.","DOI":"10.1186\/s12903-020-01140-4"},{"key":"ref_55","unstructured":"Md Ashar, N.A. (2015). Patterning of the Human Dentition: Implications for Forensic Odontology. [Ph.D. Thesis, School of Dentistry, University of Adelaide]."},{"key":"ref_56","first-page":"273","article-title":"Prevalence of torus palatinus and association with dental arch shape in a multi-ethnic cohort","volume":"71","author":"Anderton","year":"2020","journal-title":"Homo Int. Z. Vgl. Forsch. Menschen"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"eaat7487","DOI":"10.1126\/science.aat7487","article-title":"The formation of human populations in South and Central Asia","volume":"365","author":"Narasimhan","year":"2019","journal-title":"Science"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1080\/00450618.2022.2133168","article-title":"Craniofacial measurements of Malaysian Malays, Chinese and Indians based on the analyses of post-mortem computed tomographic images","volume":"56","author":"Hisham","year":"2024","journal-title":"Aust. J. Forensic Sci."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Simon, B., Mangano, F.G., P\u00e1l, A., Simon, I., Pellei, D., Shahbazi, A., and V\u00e1g, J. (2023). Palatal asymmetry assessed by intraoral scans: Effects of sex, orthodontic treatment, and twinning. A retrospective cohort study. BMC Oral Health, 23.","DOI":"10.1186\/s12903-023-02993-1"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1080\/00450618.2024.2331527","article-title":"Classification of Malaysian individuals using Fordisc 3 based on four craniofacial measurements","volume":"57","author":"Hisham","year":"2025","journal-title":"Aust. J. Forensic Sci."},{"key":"ref_61","unstructured":"Obertov\u00e1, Z., Stewart, A., and Cattaneo, C. (2020). Chapter 5.2\u2014Fordisc: Anthropological software for estimation of ancestry, sex, time period, and stature. Statistics and Probability in Forensic Anthropology, Academic Press."}],"container-title":["Dentistry Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-6767\/14\/5\/258\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T08:58:45Z","timestamp":1777453125000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-6767\/14\/5\/258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,29]]},"references-count":61,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["dj14050258"],"URL":"https:\/\/doi.org\/10.3390\/dj14050258","relation":{},"ISSN":["2304-6767"],"issn-type":[{"value":"2304-6767","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,29]]}}}