{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:53:10Z","timestamp":1785412390887,"version":"3.56.0"},"reference-count":38,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["21K17201"],"award-info":[{"award-number":["21K17201"]}]},{"name":"JSPS KAKENHI","award":["23K16233"],"award-info":[{"award-number":["23K16233"]}]},{"award":["21K17201"],"award-info":[{"award-number":["21K17201"]}],"id":[{"id":"https:\/\/ror.org\/00hhkn466","id-type":"ROR","asserted-by":"publisher"}]},{"award":["23K16233"],"award-info":[{"award-number":["23K16233"]}],"id":[{"id":"https:\/\/ror.org\/00hhkn466","id-type":"ROR","asserted-by":"publisher"}]},{"name":"NSK Nakanishi Foundation for the Research and Development"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dentistry Journal"],"abstract":"<jats:p>Background: Porphyromonas gingivalis is the principal bacterial species implicated in the pathogenesis of periodontitis. Despite its central role, direct detection of periodontopathic bacteria is not routinely performed in dental clinics. Although test kits using monoclonal antibodies against P. gingivalis have been reported, no practical chairside method has yet been established. Objectives: In this study, we developed an immunochromatography system that enables rapid and quantitative detection of P. gingivalis within 10 min. Methods: We evaluated the performance of the developed immunochromatography system and its dedicated measurement reader. Results: The kit demonstrated a minimum detection limit of 2.6 \u00d7 104 CFU\/mL and maintained detection capability across fimbrial types I and II. The dedicated reader showed strong correlations with a reference reader (R2 = 0.9921) and visual color chart evaluation (R2 = 0.9347). High intra- and inter-device precision was observed, with coefficients of variation less than 0.6%. Although the presence of 2% whole blood interfered with visual interpretation, other oral substances, including toothpastes and mouthwashes, did not significantly affect the assay performance. Conclusions: Collectively, these findings indicate that the developed system may serve as a rapid and reliable chairside tool for quantitative detection of P. gingivalis, with potential utility in the diagnosis and management of periodontitis and related systemic conditions.<\/jats:p>","DOI":"10.3390\/dj14070422","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T10:16:22Z","timestamp":1783592182000},"page":"422","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a Quantitative Detection System for Porphyromonas gingivalis Based on Immunochromatography"],"prefix":"10.3390","volume":"14","author":[{"given":"Kaoru","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Division of Infections and Molecular Biology, Department of Advanced Pathophysiological Science, School of Dentistry, Kyushu Dental University, Kitakyushu 803-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1152-3248","authenticated-orcid":false,"given":"Ryota","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Division of Infections and Molecular Biology, Department of Advanced Pathophysiological Science, School of Dentistry, Kyushu Dental University, Kitakyushu 803-8580, Japan"},{"name":"Oral Medicine Innovation Center, Kyushu Dental University, Kitakyushu 803-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5208-1848","authenticated-orcid":false,"given":"Michihiko","family":"Usui","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Department of Oral Function, School of Dentistry, Kyushu Dental University, Kitakyushu 803-8580, Japan"},{"name":"Division of Periodontology, School of Dentistry, Ohu University, Koriyama 963-8041, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Morita","sequence":"additional","affiliation":[{"name":"Division of Infections and Molecular Biology, Department of Advanced Pathophysiological Science, School of Dentistry, Kyushu Dental University, Kitakyushu 803-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Inoue","sequence":"additional","affiliation":[{"name":"Periodontal Medicine Center, Kitakyushu 806-0021, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2615-6545","authenticated-orcid":false,"given":"Yoshie","family":"Nagai-Yoshioka","sequence":"additional","affiliation":[{"name":"Division of Infections and Molecular Biology, Department of Advanced Pathophysiological Science, School of Dentistry, Kyushu Dental University, Kitakyushu 803-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5739-2936","authenticated-orcid":false,"given":"Masanori","family":"Iwasaki","sequence":"additional","affiliation":[{"name":"Division of Dental Public Health, Department of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Nakashima","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Department of Oral Function, School of Dentistry, Kyushu Dental University, Kitakyushu 803-8580, Japan"},{"name":"Periodontal Medicine Center, Kitakyushu 806-0021, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuji","family":"Nisihara","sequence":"additional","affiliation":[{"name":"Division of Infections and Molecular Biology, Department of Advanced Pathophysiological Science, School of Dentistry, Kyushu Dental University, Kitakyushu 803-8580, Japan"},{"name":"Periodontal Medicine Center, Kitakyushu 806-0021, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6533-5683","authenticated-orcid":false,"given":"Wataru","family":"Ariyoshi","sequence":"additional","affiliation":[{"name":"Division of Infections and Molecular Biology, Department of Advanced Pathophysiological Science, School of Dentistry, Kyushu Dental University, Kitakyushu 803-8580, Japan"},{"name":"Oral Medicine Innovation Center, Kyushu Dental University, Kitakyushu 803-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1016\/S0140-6736(05)67728-8","article-title":"Periodontal 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