{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T13:15:25Z","timestamp":1788527725114,"version":"build-2803163510"},"reference-count":47,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Koite Health Ltd."},{"id":[{"id":"https:\/\/ror.org\/04nstrg59","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dent. J.","Dentistry Journal"],"abstract":"<jats:p>Background\/Objectives: Patients with fixed orthodontic appliances are at increased risk of plaque accumulation, gingivitis, and caries because brackets and bands hinder mechanical hygiene. This study evaluated whether regular home use of dual-light antibacterial photodynamic therapy (aPDT) supports oral hygiene during fixed orthodontic treatment in adolescents. Methods: In a single-center, randomized, assessor-blinded trial, forty-one adolescents starting fixed orthodontic treatment were randomised to a Control group (standard hygiene) or a Test group (standard hygiene plus home-use dual-light aPDT). Visible Plaque Index (VPI%), Bleeding on Probing (BOP%), and Orthodontic Plaque Index (OPI) were assessed at baseline and 12 weeks by an assessor blinded to allocation. After two protocol-driven exclusions, 39 participants (20 Test group, 19 Control group) were analysed. Site-level proportions were compared using the chi-square test. Results: VPI decreased in both groups, with a larger mean within-group reduction in the Test group (\u22127.0%) than in the Control group (\u22123.7%); exploratory site-level analysis showed a greater reduction in plaque-positive sites in the Test group at 12 weeks (p &lt; 0.001). BOP increased in both groups but to a smaller extent in the Test group (mean +2.4% versus +5.7%), and a higher proportion of Test-group participants maintained BOP \u2264 10% (85% versus 58%); bleeding-positive sites were lower in the Test group at 12 weeks (p &lt; 0.001). The Test group also showed a flatter distribution of OPI scores around orthodontic brackets. No device- or protocol-related adverse events were reported. Conclusions: In adolescents undergoing fixed orthodontic treatment, regular home use of dual-light aPDT was safe and associated with better site-level plaque and bleeding control, with directional but non-significant trends at the participant level. The findings support further evaluation of dual-light aPDT as an adjunct to mechanical hygiene during this vulnerable treatment period.<\/jats:p>","DOI":"10.3390\/dj14090564","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T12:20:46Z","timestamp":1788524446000},"page":"564","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Efficacy of Dual-Light Photodynamic Therapy on Oral Health in Adolescents Undergoing Fixed Orthodontic Treatment: A Randomized, Assessor-Blinded and Controlled Trial"],"prefix":"10.3390","volume":"14","author":[{"given":"Katja","family":"Hashemi Elses","sequence":"first","affiliation":[{"name":"Department of Periodontology, Faculty of Odontology, Malm\u00f6 University, SE-205 06 Malm\u00f6, Sweden"},{"name":"Ortodonti Lund, Borgska Villans Specialisttandv\u00e5rd, Hedvig M\u00f6llers Gatan 17, SE-223 55 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tommi","family":"P\u00e4til\u00e4","sequence":"additional","affiliation":[{"name":"Department of Congenital Heart Surgery and Organ Transplantation, New Children\u2019s Hospital, University of Helsinki, 00290 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann-Marie","family":"Roos Jans\u00e5ker","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Odontology, Malm\u00f6 University, SE-205 06 Malm\u00f6, Sweden"},{"name":"Ortodonti Lund, Borgska Villans Specialisttandv\u00e5rd, Hedvig M\u00f6llers Gatan 17, SE-223 55 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Linden, J., Widstr\u00f6m, E., and Sinkkonen, J. (2019). Children and adolescents\u2019 dental treatment in 2001\u20132013 in the Finnish public dental service. BMC Oral Health, 19.","DOI":"10.1186\/s12903-019-0828-z"},{"key":"ref_2","first-page":"69","article-title":"The effect of orthodontic treatment on periodontal tissue inflammation: A systematic review","volume":"49","author":"Verrusio","year":"2018","journal-title":"Quintessence Int."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1053\/j.sodo.2024.01.011","article-title":"Periodontal considerations in orthodontic treatment: A review of the literature and recommended protocols","volume":"30","author":"Kwon","year":"2024","journal-title":"Semin. Orthod."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Contaldo, M., Lucchese, A., Lajolo, C., Rupe, C., Di Stasio, D., Romano, A., Petruzzi, M., and Serpico, R. (2021). The oral microbiota changes in orthodontic patients and effects on oral health: An overview. J. Clin. Med., 10.","DOI":"10.3390\/jcm10040780"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zhao, M., Yu, C., Su, C., Wang, X., Weir, M.D., Hockin, H.K.X., Liu, M., Bai, Y., and Zhang, N. (2024). Dynamic effects of fixed orthodontic treatment on oral health and oral microbiota: A prospective study. BMC Oral Health, 24.","DOI":"10.1186\/s12903-024-05356-6"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Inquimbert, C., Clement, C., Couatarmanach, A., Tramini, P., Bourgeois, D., and Carrouel, F. (2022). Oral hygiene practices and knowledge among adolescents aged between 15 and 17 years old during fixed orthodontic treatment: Multicentre study conducted in France. Int. J. Environ. Res. Public Health, 19.","DOI":"10.20944\/preprints202201.0150.v1"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.17219\/acem\/174444","article-title":"Fixed appliances orthodontic therapy as a risk factor for caries development: Systematic review","volume":"33","author":"Padella","year":"2024","journal-title":"Adv. Clin. Exp. Med."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Giannini, L., Galbiati, G., Tartaglia, F.C., Grecolini, M.E., Maspero, C., and Biagi, R. (2025). Orthodontic treatment with fixed appliances versus aligners: An experimental study of periodontal aspects. Dent. J., 13.","DOI":"10.3390\/dj13020070"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"106","DOI":"10.22271\/oral.2025.v11.i1b.2108","article-title":"To analyse and evaluate the gingival problems occurring in patients undergoing fixed orthodontic treatment: A questionnaire survey","volume":"11","author":"Banerjee","year":"2025","journal-title":"Int. J. Appl. Dent. Sci."},{"key":"ref_10","first-page":"CD012018","article-title":"Home use of interdental cleaning devices, in addition to toothbrushing, for preventing and controlling periodontal diseases and dental caries","volume":"4","author":"Worthington","year":"2019","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"125","DOI":"10.23736\/S2724-6329.22.04711-8","article-title":"Comparison of two electric toothbrushes: Evaluation on orthodontic patients","volume":"72","author":"Lanzetti","year":"2023","journal-title":"Minerva Dent. Oral Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1016\/j.ajodo.2021.02.016","article-title":"A randomized, 3-month, parallel-group clinical trial to compare the efficacy of electric 3-dimensional toothbrushes vs. manual toothbrushes in maintaining oral health in patients with fixed orthodontic appliances","volume":"160","author":"Mylonopoulou","year":"2021","journal-title":"Am. J. Orthod. Dentofac. Orthop."},{"key":"ref_13","first-page":"S9","article-title":"Periodontal health","volume":"89","author":"Lang","year":"2018","journal-title":"J. Periodontol."},{"key":"ref_14","first-page":"e38231","article-title":"The efficacy of the three types of plaque control methods during fixed orthodontic treatment: A randomized controlled trial","volume":"15","author":"Kumar","year":"2023","journal-title":"Cureus"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"103497","DOI":"10.1016\/j.jdent.2020.103497","article-title":"Current uses of chlorhexidine for management of oral disease: A narrative review","volume":"103","author":"Brookes","year":"2020","journal-title":"J. Dent."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.identj.2022.01.005","article-title":"Chlorhexidine in dentistry: Pharmacology, uses, and adverse effects","volume":"72","author":"Ouanounou","year":"2022","journal-title":"Int. Dent. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1111\/j.1600-051X.2012.01883.x","article-title":"Effect of a chlorhexidine mouthrinse on plaque, gingival inflammation and staining in gingivitis patients: A systematic review","volume":"39","author":"Slot","year":"2012","journal-title":"J. Clin. Periodontol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Palanisamy, S. (2024). Innovations in oral hygiene tools: A mini review on recent developments. Front. Dent. Med., 5.","DOI":"10.3389\/fdmed.2024.1442887"},{"key":"ref_19","first-page":"783","article-title":"Antimicrobial photodynamic therapy for the treatment of periodontitis and peri-implantitis: An American Academy of Periodontology best evidence review","volume":"89","author":"Chambrone","year":"2018","journal-title":"J. Periodontol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Ferreira, R., Greghi, S.L.A., Sant\u2019Ana, A.C.P., Zangrando, M.S.R., and Damante, C.A. (2025). Multiple sessions of antimicrobial photodynamic therapy improve periodontal outcomes in patients with Down syndrome: A randomized clinical trial. Dent. J., 13.","DOI":"10.3390\/dj13010033"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Mahdizade Ari, M., Amirmozafari, N., Darbandi, A., Afifrad, R., Asadollahi, P., and Iraijan, G. (2024). Effectiveness of photodynamic therapy on the treatment of chronic periodontitis: Systematic review during 2008\u20132013. Front. Chem., 12.","DOI":"10.3389\/fchem.2024.1384344"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1038\/sj.ebd.6400791","article-title":"Insufficient evidence for photodynamic therapy use in periodontitis","volume":"12","author":"Herrera","year":"2011","journal-title":"Evid. Based Dent."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Nikinmaa, S., Moilanen, N., Sorsa, T., Rantala, J., Alapulli, H., Kotiranta, A., Auvinen, P., Kankuri, E., Meurman, J.H., and P\u00e4til\u00e4, T. (2021). Indocyanine green-assisted and LED-light-activated antibacterial photodynamic therapy reduces dental plaque. Dent. J., 9.","DOI":"10.3390\/dj9050052"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e70146","DOI":"10.1002\/cre2.70146","article-title":"Peri-implant diseases: Enhanced antibacterial photodynamic therapy","volume":"11","author":"Kailliala","year":"2025","journal-title":"Clin. Exp. Dent. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1002\/jper.70082","article-title":"A randomized controlled trial of home-applied dual-light photodynamic therapy during supportive periodontal care (HOPE-CP study)","volume":"97","author":"Pakarinen","year":"2026","journal-title":"J. Periodontol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"106641","DOI":"10.1016\/j.jdent.2026.106641","article-title":"Plaque control by dual-light photodynamic therapy in healthy adults: A randomized crossover pilot study","volume":"172","author":"Gusseva","year":"2026","journal-title":"J. Dent."},{"key":"ref_27","unstructured":"(2020). Clinical investigation of medical devices for human subjects\u2014Good clinical practice (Standard No. Standard\u2019s ISO 14155:2020)."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Nikinmaa, S., Alapulli, H., Auvinen, P., Vaara, M., Rantala, J., Kankuri, E., Sorsa, T., Meurman, J., and P\u00e4til\u00e4, T. (2020). Dual-light photodynamic therapy administered daily provides a sustained antibacterial effect on biofilm and prevents Streptococcus mutans adaptation. PLoS ONE, 15.","DOI":"10.1101\/2020.01.09.899963"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Nikinmaa, S., Podonyi, A., Raivio, P., Meurman, J., Sorsa, T., Rantala, J., Kankuri, E., Tauriainen, T., and P\u00e4til\u00e4, T. (2021). Daily administered dual-light photodynamic therapy provides a sustained antibacterial effect on Staphylococcus aureus. Antibiotics, 10.","DOI":"10.3390\/antibiotics10101240"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"484","DOI":"10.18433\/jpps32084","article-title":"Dual-light photodynamic therapy effectively eliminates Streptococcus oralis biofilms","volume":"24","author":"Laakamaa","year":"2021","journal-title":"J. Pharm. Pharm. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.12688\/f1000research.140950.1","article-title":"A systematic review of trends in photobiomodulation in dentistry between 2018 and 2022","volume":"12","year":"2023","journal-title":"F1000Research"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Dompe, C., Moncrieff, L., Matys, J., Grzech-Le\u015bniak, K., Kocherova, I., Bryja, A., Bruska, M., Dominiak, M., Mozdziak, P., and Skiba, T.H.I. (2020). Photobiomodulation\u2014Underlying mechanism and clinical applications. J. Clin. Med., 9.","DOI":"10.3390\/jcm9061724"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1902\/jop.1972.43.1.38","article-title":"The plaque control record","volume":"43","author":"Drake","year":"1972","journal-title":"J. Periodontol."},{"key":"ref_34","first-page":"229","article-title":"Problems and proposals for recording gingivitis and plaque","volume":"25","author":"Ainamo","year":"1975","journal-title":"Int. Dent. J."},{"key":"ref_35","first-page":"94","article-title":"The orthodontic plaque index: An oral hygiene index for patients with multibracket appliances","volume":"13","author":"Beberhold","year":"2012","journal-title":"Orthodontics"},{"key":"ref_36","first-page":"7","article-title":"Periodontal risk assessment (PRA) for patients in supportive periodontal therapy (SPT)","volume":"1","author":"Lang","year":"2003","journal-title":"Oral Health Prev. Dent."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"S71","DOI":"10.1111\/jcpe.12366","article-title":"Primary prevention of periodontitis: Managing gingivitis","volume":"42","author":"Chapple","year":"2015","journal-title":"J. Clin. Periodontol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"S37","DOI":"10.1111\/adj.12671","article-title":"Prevention and caries risk management in teenage and orthodontic patients","volume":"64","author":"Walsh","year":"2019","journal-title":"Aust. Dent. J."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Spatafora, G., Li, Y., He, X., Cowan, A., and Tanner, A.C.R. (2024). The evolving microbiome of dental caries. Microorganisms, 12.","DOI":"10.3390\/microorganisms12010121"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s41547-019-00056-9","article-title":"Photobiomodulation therapy in the treatment of periodontal disease: A literature review","volume":"3","author":"Alaijah","year":"2019","journal-title":"Laser Dent. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1007\/s00284-025-04648-6","article-title":"The role of the oral microbiome in periodontal disease: A systematic review of microbial associations and therapeutic implications","volume":"83","author":"Alavi","year":"2026","journal-title":"Curr. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Peterson, B.W., Tjakkes, G.H., Renkema, A.M., Manton, D.J., and Ren, Y. (2024). The oral microbiota and periodontal health in orthodontic patients. Periodontology 2000, Online ahead of Print.","DOI":"10.1111\/prd.12594"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2372751","DOI":"10.1080\/20002297.2024.2372751","article-title":"A comparison of the alterations of oral microbiome with fixed orthodontic therapy and clear aligners: A systematic review","volume":"17","author":"Lucchese","year":"2025","journal-title":"J. Oral Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.jphotobiol.2004.11.023","article-title":"Photodynamic therapy for periodontal diseases: State of the art","volume":"79","author":"Meisel","year":"2005","journal-title":"J. Photochem. Photobiol. B"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"117962","DOI":"10.1016\/j.bmc.2024.117962","article-title":"Photodynamic therapy: An emerging therapeutic modality in dentistry","volume":"114","author":"Suresh","year":"2024","journal-title":"Bioorg. Med. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Tegelberg, P., Ojala, M., Ylipalosaari, M., Lindroth, J., Heikkinen, A.M., L\u00e4hteenm\u00e4ki, H., Pakarinen, S., R\u00e4is\u00e4nen, I.T., Sorsa, T., and P\u00e4til\u00e4, T. (2026). A multicenter, controlled, randomized trial of home-applied dual-light photodynamic therapy in stage I to III periodontitis (LumoNorth2022 study). J. Periodontol., Online ahead of Print.","DOI":"10.1002\/jper.70120"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Hopefell, S., Chan, A., Collins, G.S., Hr\u00f3bjartsson, A., Moher, D., Schulz, K.F., Tunn, R., Aggarwal, R., Berkwits, M., and Berlin, J.A. (2025). CONSORT 2025 statement: Updated guideline for reporting randomised trials\n          . 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