{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:08:57Z","timestamp":1789168137209,"version":"build-2803163510"},"reference-count":46,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Kyushu Dental University","award":["Promotion Program for Strategic Research 2019"],"award-info":[{"award-number":["Promotion Program for Strategic Research 2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dentistry Journal"],"abstract":"<jats:p>This study aimed to explore whether the Trypsin-Like Peptidase Activity Assay Kit (TLP-AA-Kit), which measures the activity of N-benzoyl-dl-arginine peptidase (trypsin-like peptidase), can be used as a reliable tool for periodontitis detection in population-based surveillance. In total, 105 individuals underwent a full-mouth periodontal examination and provided tongue swabs as specimens for further analyses. The results of the TLP-AA-Kit were scored between 1 and 5; higher scores indicated higher trypsin concentrations. Receiver operating characteristic analyses were used to evaluate the predictive validity of the TLP-AA-Kit, where the periodontitis case definition provided by the Centers for Disease Control\/American Academy of Periodontology served as the reference. Severe and moderate periodontitis were identified in 4.8% and 16.2% of the study population, respectively. The TLP-AA-Kit showed high diagnostic accuracy for severe periodontitis, with an area under the curve of 0.93 (95% confidence interval = 0.88\u20130.99). However, the diagnostic accuracy of the TLP-AA-Kit for moderate\/severe periodontitis was not reliable. While further studies are necessary to validate our results, the results provided herein highlight the potential of the TLP-AA-Kit as a useful tool for the detection of periodontitis, particularly in severe cases, for population-based surveillance.<\/jats:p>","DOI":"10.3390\/dj8030098","type":"journal-article","created":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T08:53:43Z","timestamp":1598950423000},"page":"98","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A Preliminary Study on the Ability of the Trypsin-Like Peptidase Activity Assay Kit to Detect Periodontitis"],"prefix":"10.3390","volume":"8","author":[{"given":"Masanori","family":"Iwasaki","sequence":"first","affiliation":[{"name":"Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-Ku, Tokyo 173-0015, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5208-1848","authenticated-orcid":false,"given":"Michihiko","family":"Usui","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Kyushu Dental University, Kitakyushu 803-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6533-5683","authenticated-orcid":false,"given":"Wataru","family":"Ariyoshi","sequence":"additional","affiliation":[{"name":"Division of Infections and Molecular Biology, Kyushu Dental University, Kitakyushu 803-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Nakashima","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Kyushu Dental University, Kitakyushu 803-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshie","family":"Nagai-Yoshioka","sequence":"additional","affiliation":[{"name":"Division of Infections and Molecular Biology, Kyushu Dental University, Kitakyushu 803-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Inoue","sequence":"additional","affiliation":[{"name":"Division of Infections and Molecular Biology, Kyushu Dental University, Kitakyushu 803-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuji","family":"Nishihara","sequence":"additional","affiliation":[{"name":"Division of Infections and Molecular Biology, Kyushu Dental University, Kitakyushu 803-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,1]]},"reference":[{"key":"ref_1","unstructured":"The Ministry of Health, Labour, and Welfare (2020, August 02). 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