{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T17:30:23Z","timestamp":1784050223539,"version":"3.55.0"},"reference-count":136,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T00:00:00Z","timestamp":1680134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 ES028865"],"award-info":[{"award-number":["R01 ES028865"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["DNA"],"abstract":"<jats:p>Nucleic acid methylations are important genetic and epigenetic biomarkers. The formation and removal of these markers is related to either methylation or demethylation. In this review, we focus on the demethylation or oxidative modification that is mediated by the 2-oxoglutarate (2-OG)\/Fe(II)-dependent AlkB\/TET family enzymes. In the catalytic process, most enzymes oxidize 2-OG to succinate, in the meantime oxidizing methyl to hydroxymethyl, leaving formaldehyde and generating demethylated base. The AlkB enzyme from Escherichia coli has nine human homologs (ALKBH1-8 and FTO) and the TET family includes three members, TET1 to 3. Among them, some enzymes have been carefully studied, but for certain enzymes, few studies have been carried out. This review focuses on the kinetic properties of those 2-OG\/Fe(II)-dependent enzymes and their alkyl substrates. We also provide some discussions on the future directions of this field.<\/jats:p>","DOI":"10.3390\/dna3020005","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T03:02:01Z","timestamp":1680231721000},"page":"65-84","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Kinetic Studies on the 2-Oxoglutarate\/Fe(II)-Dependent Nucleic Acid Modifying Enzymes from the AlkB and TET Families"],"prefix":"10.3390","volume":"3","author":[{"given":"Zhiyuan","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christo Z.","family":"Christov","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Michigan Technological University, Houghton, MI 49931, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatyana","family":"Karabencheva-Christova","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Michigan Technological University, Houghton, MI 49931, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5221-5498","authenticated-orcid":false,"given":"Nicolai","family":"Lehnert","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Department of Biophysics, University of Michigan, Ann Arbor, MI 48109, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1082-0569","authenticated-orcid":false,"given":"Deyu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1002\/j.1460-2075.1982.tb01149.x","article-title":"Nonenzymatic Methylation of DNA by the Intracellular Methyl Group Donor S-Adenosyl-L-Methionine Is a Potentially Mutagenic Reaction","volume":"1","author":"Rydberg","year":"1982","journal-title":"EMBO J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1080\/08958370490464553","article-title":"DNA Methylation, Cell Proliferation, and Histopathology in Rats Following Repeated Inhalation Exposure to Dimethyl Sulfate","volume":"16","author":"Mathison","year":"2004","journal-title":"Inhal. 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