{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T04:22:11Z","timestamp":1787458931454,"version":"build-2736575974"},"reference-count":46,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004257","name":"Western Norway Regional Health Trust","doi-asserted-by":"publisher","award":["F-12591"],"award-info":[{"award-number":["F-12591"]}],"id":[{"id":"10.13039\/501100004257","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019845","name":"U.S.-Norway Fulbright Foundation for Educational Exchange","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100019845","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/04zbwa870","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur. Burn J.","EBJ"],"abstract":"<jats:p>Background: Norepinephrine is commonly used during acute burn resuscitation to maintain adequate perfusion pressure. However, its effects, together with the burn-induced catecholamine surge, on arterial \u03b11-adrenergic receptors (\u03b11-AR) remain unclear. This study examined whether burn injury and continuous norepinephrine infusion alter arterial \u03b11-AR subtype expression at the transcriptional and protein levels. Methods: Sprague\u2013Dawley rats were randomized to full-thickness scald burn involving 25% total body surface area with continuous intravenous norepinephrine or sodium chloride (NaCl) infusion, sham injury with norepinephrine infusion, or untreated controls. Mean arterial pressure (MAP) was measured at baseline and before euthanasia (six or 24 h). Aorta, carotid, and renal arteries were collected for analysis of \u03b11-AR subtypes (Adra1a, Adra1b, and Adra1d) using quantitative PCR (qPCR) and immunohistochemistry (IHC). Results: Norepinephrine efficiently increased MAP in sham animals. In burned animals with norepinephrine, MAP increased at six hours (+10.6 mmHg) but fell below baseline at 24 h (\u22126.4 mmHg). Burned animals given NaCl had persistently lower MAP than those given norepinephrine, at six hours (\u221219.1 mmHg) and 24 h (\u221221.1 mmHg). qPCR demonstrated significant downregulation of Adra1b in the renal artery across all groups compared to control (7.8\u201316.4-fold, p &lt; 0.001). Overall, changes in protein expression across different vascular beds and receptor subtypes were inconsistent. Conclusions: Norepinephrine increased MAP in both sham and burned animals, confirming the drug\u2019s efficacy. Burn injury and sustained norepinephrine exposure were associated with early, vessel-specific alterations in \u03b11-AR mRNA expression. The absence of consistent protein-level changes within 24 h suggests a delay in receptor expression on protein level.<\/jats:p>","DOI":"10.3390\/ebj7030045","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T08:14:01Z","timestamp":1787300041000},"page":"45","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Severe Burn Injury Alters Expression of Adrenergic Receptor Transcripts in a Rodent Model"],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1016-2113","authenticated-orcid":false,"given":"Kristine","family":"Knappskog","sequence":"first","affiliation":[{"name":"Research Group for Burn Care and Reconstructive Surgery, Department of Clinical Medicine, Faculty of Medicine, University of Bergen, 5009 Bergen, Norway"},{"name":"Department of Surgery, The University of Texas Medical Branch, Galveston, TX 77555, USA"},{"name":"Norwegian National Burn Center, Department of Plastic, Hand, and Reconstructive Surgery, Haukeland University Hospital, 5021 Bergen, Norway"},{"name":"Department of Medical Genetics, Haukeland University Hospital, 5021 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2487-9009","authenticated-orcid":false,"given":"Julia","family":"Kleinhapl","sequence":"additional","affiliation":[{"name":"Department of Surgery, The University of Texas Medical Branch, Galveston, TX 77555, USA"},{"name":"Division of Plastic, Aesthetic and Reconstructive Surgery, Department of Surgery, Medical University of Graz, 8010 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8595-4489","authenticated-orcid":false,"given":"Titas","family":"Gladkauskas","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Haukeland University Hospital, 5021 Bergen, Norway"},{"name":"Department of Clinical Medicine, Faculty of Medicine, University of Bergen, 5020 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siren","family":"Fromreide","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Faculty of Medicine, University of Bergen, 5020 Bergen, Norway"},{"name":"Centre for Cancer Biomarkers (CCBIO), University of Bergen, 5020 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0903-1140","authenticated-orcid":false,"given":"Dagrun Sletteb\u00f8","family":"Daltveit","sequence":"additional","affiliation":[{"name":"Department of Global Public Health and Primary Care, University of Bergen, 5020 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2382-9594","authenticated-orcid":false,"given":"Jake E.","family":"Lowry","sequence":"additional","affiliation":[{"name":"Animal Resource Center, The University of Texas Medical Branch at Galveston, Galveston, TX 77555, USA"},{"name":"Department of Comparative Medicine, The University of Texas MD Anderson Cancer Center, Galveston, TX 77555, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per Morten","family":"Knappskog","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Haukeland University Hospital, 5021 Bergen, Norway"},{"name":"Department of Clinical Science, University of Bergen, 5021 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4478-9539","authenticated-orcid":false,"given":"Henning","family":"Onarheim","sequence":"additional","affiliation":[{"name":"Research Group for Burn Care and Reconstructive Surgery, Department of Clinical Medicine, Faculty of Medicine, University of Bergen, 5009 Bergen, Norway"},{"name":"Norwegian National Burn Center, Department of Plastic, Hand, and Reconstructive Surgery, Haukeland University Hospital, 5021 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne Berit","family":"Guttormsen","sequence":"additional","affiliation":[{"name":"Research Group for Burn Care and Reconstructive Surgery, Department of Clinical Medicine, Faculty of Medicine, University of Bergen, 5009 Bergen, Norway"},{"name":"Department of Anesthesiology and Intensive Care, Haukeland University Hospital, 5021 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7673-0358","authenticated-orcid":false,"given":"Daniela Elena","family":"Costea","sequence":"additional","affiliation":[{"name":"Centre for Cancer Biomarkers (CCBIO), University of Bergen, 5020 Bergen, Norway"},{"name":"Department of Pathology, Haukeland University Hospital, 5021 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3657-0633","authenticated-orcid":false,"given":"Amina","family":"El Ayadi","sequence":"additional","affiliation":[{"name":"Department of Surgery, The University of Texas Medical Branch, Galveston, TX 77555, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9371-9078","authenticated-orcid":false,"given":"Juquan","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Surgery, The University of Texas Medical Branch, Galveston, TX 77555, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2972-3440","authenticated-orcid":false,"given":"Steven E.","family":"Wolf","sequence":"additional","affiliation":[{"name":"Department of Surgery, The University of Texas Medical Branch, Galveston, TX 77555, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5992-4791","authenticated-orcid":false,"given":"Stian Kreken","family":"Almeland","sequence":"additional","affiliation":[{"name":"Research Group for Burn Care and Reconstructive Surgery, Department of Clinical Medicine, Faculty of Medicine, University of Bergen, 5009 Bergen, Norway"},{"name":"Norwegian National Burn Center, Department of Plastic, Hand, and Reconstructive Surgery, Haukeland University Hospital, 5021 Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1097\/00000658-195704000-00004","article-title":"Urinary output of adrenaline and noradrenaline in severe thermal burns","volume":"145","author":"Goodall","year":"1957","journal-title":"Ann. 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Care Med."}],"container-title":["European Burn Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2673-1991\/7\/3\/45\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T04:12:24Z","timestamp":1787458344000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2673-1991\/7\/3\/45"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,21]]},"references-count":46,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ebj7030045"],"URL":"https:\/\/doi.org\/10.3390\/ebj7030045","relation":{},"ISSN":["2673-1991"],"issn-type":[{"value":"2673-1991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,21]]}}}