{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T14:07:52Z","timestamp":1764079672095,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,6,17]],"date-time":"2021-06-17T00:00:00Z","timestamp":1623888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["247690"],"award-info":[{"award-number":["247690"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006356","name":"Syddansk Universitet","doi-asserted-by":"publisher","award":["38-2-4-17 BIOCAS"],"award-info":[{"award-number":["38-2-4-17 BIOCAS"]}],"id":[{"id":"10.13039\/501100006356","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Energies"],"abstract":"<jats:p>In this paper, a study on the feasibility of the treatment of raw cheese whey by anaerobic co-digestion using coffee pulp residues as a co-substrate is presented. It considers raw whey generated in artisanal cheese markers, which is generally not treated, thus causing environmental pollution problems. An experimental design was carried out evaluating the effect of pH and the substrate ratio on methane production at 35 \u00b0C (i.e., mesophilic conditions). The interaction of the parameters on the co-substrate degradation and the methane production was analyzed using a response surface analysis. Furthermore, two kinetic models were proposed (first order and modified Gompertz models) to determine the dynamic profiles of methane yield. The results show that co-digestion of the raw whey is favored at pH = 6, reaching a maximum yield of 71.54 mLCH4 gVSrem\u22121 (31.5% VS removed) for raw cheese whey and coffee pulp ratio of 1 gVSwhey gVSCoffe\u22121. The proposed kinetic models successfully fit the experimental methane production data, the Gompertz model being the one that showed the best fit. Then, the results show that anaerobic co-digestion can be used to reduce the environmental impact of raw whey. Likewise, the methane obtained can be integrated into the cheese production process, which could contribute to reducing the cost per energy consumption.<\/jats:p>","DOI":"10.3390\/en14123611","type":"journal-article","created":{"date-parts":[[2021,6,17]],"date-time":"2021-06-17T11:20:26Z","timestamp":1623928826000},"page":"3611","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A Study on the Feasibility of Anaerobic Co-Digestion of Raw Cheese Whey with Coffee Pulp Residues"],"prefix":"10.3390","volume":"14","author":[{"given":"Sandra","family":"Gonzalez-Piedra","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad Veracruzana, Xalapa Veracruz 91000, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7147-4455","authenticated-orcid":false,"given":"H\u00e9ctor","family":"Hern\u00e1ndez-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto Tecnol\u00f3gico Superior de Xalapa, Xalapa Veracruz 91096, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan M.","family":"Perez-Morales","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad Veracruzana, Xalapa Veracruz 91000, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3448-8094","authenticated-orcid":false,"given":"Laura","family":"Acosta-Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad Veracruzana, Xalapa Veracruz 91000, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan-Rodrigo","family":"Bastidas-Oyanedel","sequence":"additional","affiliation":[{"name":"SDU-IGT, SDU-Biotechnology, Department of Green Technology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliseo","family":"Hernandez-Martinez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad Veracruzana, Xalapa Veracruz 91000, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,17]]},"reference":[{"key":"ref_1","unstructured":"FAO (2021, January 10). Food and Agriculture Organization of the United Nations Statistics Division. Available online: http:\/\/www.fao.org\/faostat\/en\/#data\/QP\/visualize."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jenvman.2012.05.018","article-title":"Cheese whey management: A review","volume":"110","author":"Prazeres","year":"2012","journal-title":"J. Environ. Manag."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0960-8524(96)00036-3","article-title":"The biotechnological utilization of cheese whey: A review","volume":"57","author":"Siso","year":"1996","journal-title":"Bioresour. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Chalermthai, B., Chan, W.Y., Bastidas-Oyanedel, J.R., Taher, H., Olsen, B.D., and Schmidt, J.S. (2019). Preparation and characterization of whey protein-based polymers produced from residual dairy streams. Polymers, 11.","DOI":"10.3390\/polym11040722"},{"key":"ref_5","unstructured":"Cigolotti, V., Moreno, A., and McPhail, S.J. (2012). Fuel cells. Green Energy and Technology, Springer."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/j.rser.2015.02.032","article-title":"Review on research achievements of biogas from anaerobic digestion","volume":"45","author":"Mao","year":"2015","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S1364-0321(99)00014-3","article-title":"State-of-the-art of anaerobic digestion technology for industrial wastewater treatment","volume":"4","author":"Rajeshwari","year":"2000","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2102","DOI":"10.1016\/j.biortech.2006.08.013","article-title":"Anaerobic membrane reactor with phase separation for the treatment of cheese whey","volume":"98","author":"Saddoud","year":"2007","journal-title":"Bioresour. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5227","DOI":"10.1021\/ie071622x","article-title":"Biohydrogen and methane production from cheese whey in a two-stage anaerobic process","volume":"47","author":"Antonopoulou","year":"2008","journal-title":"Ind. Eng. Chem. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3713","DOI":"10.1016\/j.biortech.2009.01.025","article-title":"Using cheese whey for hydrogen and methane generation in a two-stage continuous process with alternative pH controlling approaches","volume":"100","author":"Venetsaneas","year":"2009","journal-title":"Bioresour. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.biombioe.2015.05.024","article-title":"Thermophilic anaerobic digestion of cheese whey: Coupling H2 and CH4 production","volume":"81","author":"Cuetos","year":"2015","journal-title":"Biomass Bioenergy"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/j.wasman.2017.09.026","article-title":"Anaerobic digestion of cheese whey: Energetic and nutritional potential for the dairy sector in developing countries","volume":"71","author":"Escalante","year":"2018","journal-title":"Waste Manag."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Rabii, A., Aldin, S., Dahman, Y., and Elbeshbishy, E. (2019). A review on anaerobic co-digestion with a focus on the microbial populations and the effect of multi-stage digester configuration. Energies, 12.","DOI":"10.3390\/en12061106"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.1016\/j.renene.2006.11.015","article-title":"Optimization of biogas production by co-digesting whey with diluted poultry manure","volume":"32","author":"Gelegenis","year":"2007","journal-title":"Renew. Energy"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1016\/j.biombioe.2010.04.006","article-title":"Biogas production from co-digestion of a mixture of cheese whey and dairy manure","volume":"34","author":"Kavacik","year":"2010","journal-title":"Biomass Bioenergy"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Hallaji, S.M., Kuroshkarim, M., and Moussavi, S.P. (2019). Enhancing methane production using anaerobic co-digestion of waste activated sludge with combined fruit waste and cheese whey. BMC Biotechnol., 19.","DOI":"10.1186\/s12896-019-0513-y"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.biortech.2012.03.011","article-title":"Biomethanation potential of macroalgae Ulva spp. and Gracilaria spp. and in co-digestion with waste activated sludge","volume":"114","author":"Costa","year":"2012","journal-title":"Bioresour. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.biortech.2018.11.065","article-title":"Modeling the anaerobic co-digestion of solid waste: From batch to semi-continuous simulation","volume":"274","author":"Kouas","year":"2019","journal-title":"Bioresour. Technol."},{"key":"ref_19","first-page":"18","article-title":"Design and Development of Biogas Production System from Waste Coffee Pulp and its Waste Water Around Tepi","volume":"6","author":"Tadesse","year":"2017","journal-title":"Int. J. Recent Dev. Eng. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S1369-703X(00)00084-X","article-title":"Biotechnological potential of coffee pulp and coffee husk for bioprocesses","volume":"6","author":"Pandey","year":"2000","journal-title":"Biochem. Eng. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1016\/j.rser.2011.11.035","article-title":"Methane production from lignocellulosic agricultural crop wastes: A review in context to second generation of biofuel production","volume":"16","author":"Chandra","year":"2012","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_22","unstructured":"American Public Health Association, American Water Works Association, and Water Pollution Control Federation (2012). Standard Methods for the Examination of Water and Wastewater, American Public Health Association."},{"key":"ref_23","unstructured":"HACH (2000). Chemical Oxygen Demand, Reactor Digestion Method; Method 8000, HACH."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1021\/ac60111a017","article-title":"Colorimetric method for determination of sugars and related substances","volume":"28","author":"Dubois","year":"1956","journal-title":"Anal. Chem."},{"key":"ref_25","unstructured":"Jenkins, S.R., Morgan, J.M., and Sawyer, C.L. (1983). Measuring anaerobic sludge digestion and growth by a simple alkalimetric titration. J. Water Pollut. Control Fed., 448\u2013453."},{"key":"ref_26","unstructured":"Wellinger, A., Murphy, J., and David, B. (2013). Analysis and characterisation of biogas feedstocks. The Biogas Handbook, Woodhead Publishing."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"7015","DOI":"10.1080\/19443994.2013.823116","article-title":"Specific methanogenic activity test for anaerobic treatment of phenolic wastewater","volume":"52","author":"Hussain","year":"2014","journal-title":"Desalination Water Treat."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.btre.2014.10.005","article-title":"Theoretical methane production generated by the co-digestion of organic fraction municipal solid waste and biological sludge","volume":"5","author":"Nielfa","year":"2015","journal-title":"Biotechnol. Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.wasman.2014.12.027","article-title":"Effect of initial pH on anaerobic co-digestion of kitchen waste and cow manure","volume":"38","author":"Zhai","year":"2015","journal-title":"Waste Manag."},{"key":"ref_30","first-page":"1","article-title":"Whey based beverage: Its functionality, formulations, health benefits and applications","volume":"6","author":"Chavan","year":"2015","journal-title":"J. Food Process. Technol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1007\/s12257-012-0044-z","article-title":"Optimization of biogas production from co-digestion of whey and cow manure","volume":"17","author":"Hublin","year":"2012","journal-title":"Biotechnol. Bioprocess Eng."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.enconman.2013.07.017","article-title":"Generation of biogas from coffee-pulp and cow-dung co-digestion: Infrared studies of postcombustion emissions","volume":"74","author":"Corro","year":"2013","journal-title":"Energy Convers. Manag."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"549","DOI":"10.15381\/rivep.v28i3.13362","article-title":"Efecto de la Alimentaci\u00f3n con Pulpa de Caf\u00e9 (Coffea arabica) en los \u00cdndices Productivos de Cuyes (Cavia porcellus L) Raza Per\u00fa","volume":"28","author":"Yoplac","year":"2017","journal-title":"Rev. Investig. Vet. Del Per\u00fa"},{"key":"ref_34","first-page":"165","article-title":"Bromatological characterization of coffee (Coffea arabica L.) pulp for animal feeding purposes","volume":"52","author":"Aguirre","year":"2018","journal-title":"Cuba, J. Agric. Sci."},{"key":"ref_35","unstructured":"Lossie, U., and P\u00fctz, P. (2021, January 10). Control orientado de plantas de biog\u00e1s con la ayuda de FOS\/TAC. HACH CANGE 2011. Available online: https:\/\/de.hach.com\/asset-get.download.jsa?id=25593611197."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.rser.2014.03.041","article-title":"Process characteristics, inhibition factors and methane yields of anaerobic digestion process, with particular focus on microalgal biomass fermentation","volume":"34","author":"Kwietniewska","year":"2014","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1946","DOI":"10.1002\/jctb.6391","article-title":"Methanogenesis of raw cheese whey: PH and substrate\u2013inoculum ratio evaluation at mesophyll temperature range","volume":"95","year":"2020","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.psep.2019.05.025","article-title":"Investigating the performance of internet of things based anaerobic digestion of food waste","volume":"127","author":"Logan","year":"2019","journal-title":"Process. Saf. Environ. Prot."}],"container-title":["Energies"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1996-1073\/14\/12\/3611\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:17:40Z","timestamp":1760163460000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1996-1073\/14\/12\/3611"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,17]]},"references-count":38,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["en14123611"],"URL":"https:\/\/doi.org\/10.3390\/en14123611","relation":{},"ISSN":["1996-1073"],"issn-type":[{"type":"electronic","value":"1996-1073"}],"subject":[],"published":{"date-parts":[[2021,6,17]]}}}