{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T07:23:41Z","timestamp":1790321021165,"version":"4.1.0"},"reference-count":59,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,17]],"date-time":"2021-02-17T00:00:00Z","timestamp":1613520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["RTA2015-00005-C02-02"],"award-info":[{"award-number":["RTA2015-00005-C02-02"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007652","name":"Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria","doi-asserted-by":"publisher","award":["RTA2015-00005-C02-02"],"award-info":[{"award-number":["RTA2015-00005-C02-02"]}],"id":[{"id":"10.13039\/100007652","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Bordeaux Metabolome Facility and MetaboHUB","award":["ANR-11-INBS-0010 project"],"award-info":[{"award-number":["ANR-11-INBS-0010 project"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Genotoxic data of substances that could be used as food additives are required by the European Food Safety Authority. In this sense, the use of an extract from grapevine shoots containing a stilbene richness of 99% (ST-99), due to its antioxidant and antibacterial activities, has been proposed as an alternative to sulfur dioxide in wine. The aim of this work was to study, for the first time, the in vivo genotoxic effects produced in rats orally exposed to 90, 180, or 360 mg ST-99\/kg body weight at 0, 24, and 45 h. The combination of micronucleus assay in bone marrow (OECD 474) and standard (OECD 489) and enzyme-modified comet assay was used to determine the genotoxicity on cells isolated from stomach, liver, and blood of exposed animals. The ST-99 revealed no in vivo genotoxicity. These results were corroborated by analytical studies that confirm the presence of stilbenes and their metabolites in plasma and tissues. Moreover, to complete these findings, a histopathological study was performed under light microscopy in liver and stomach showing only slight modifications in both organs at the highest concentration used. The present work confirms that this extract is not genotoxic presenting a good profile for its potential application as a preservative in the wine industry.<\/jats:p>","DOI":"10.3390\/foods10020439","type":"journal-article","created":{"date-parts":[[2021,2,17]],"date-time":"2021-02-17T21:35:42Z","timestamp":1613597742000},"page":"439","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["In Vivo Genotoxicity Evaluation of a Stilbene Extract Prior to Its Use as a Natural Additive: A Combination of the Micronucleus Test and the Comet Assay"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6358-3694","authenticated-orcid":false,"given":"Concepci\u00f3n","family":"Medrano-Padial","sequence":"first","affiliation":[{"name":"Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, C\/Profesor Garc\u00eda Gonz\u00e1lez n\u00b02, 41012 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Puerto","sequence":"additional","affiliation":[{"name":"Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, C\/Profesor Garc\u00eda Gonz\u00e1lez n\u00b02, 41012 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5157-4763","authenticated-orcid":false,"given":"Ana Isabel","family":"Prieto","sequence":"additional","affiliation":[{"name":"Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, C\/Profesor Garc\u00eda Gonz\u00e1lez n\u00b02, 41012 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4534-7545","authenticated-orcid":false,"given":"Nah\u00fam","family":"Ayala","sequence":"additional","affiliation":[{"name":"Veterinary Faculty, Universidad de C\u00f3rdoba, Campus de Rabanales, Edificio de Sanidad Animal, 14071 C\u00f3rdoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Beaumont","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche \u0152nologie, Mol\u00e9cules d\u2019Int\u00e9r\u00eat Biologique, EA4577, USC 1366 INRAE, Universit\u00e9 de Bordeaux, Institut des Sciences de la Vigne et du Vin, 210 Chemin de Leysotte, 33882 Villenave d\u2019Ornon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6442-6567","authenticated-orcid":false,"given":"Caroline","family":"Rouger","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche \u0152nologie, Mol\u00e9cules d\u2019Int\u00e9r\u00eat Biologique, EA4577, USC 1366 INRAE, Universit\u00e9 de Bordeaux, Institut des Sciences de la Vigne et du Vin, 210 Chemin de Leysotte, 33882 Villenave d\u2019Ornon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6509-8772","authenticated-orcid":false,"given":"St\u00e9phanie","family":"Krisa","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche \u0152nologie, Mol\u00e9cules d\u2019Int\u00e9r\u00eat Biologique, EA4577, USC 1366 INRAE, Universit\u00e9 de Bordeaux, Institut des Sciences de la Vigne et du Vin, 210 Chemin de Leysotte, 33882 Villenave d\u2019Ornon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5765-1942","authenticated-orcid":false,"given":"Silvia","family":"Pichardo","sequence":"additional","affiliation":[{"name":"Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, C\/Profesor Garc\u00eda Gonz\u00e1lez n\u00b02, 41012 Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.tifs.2015.06.007","article-title":"Natural Food Additives: Quo Vadis?","volume":"45","author":"Carocho","year":"2015","journal-title":"Trends Food Sci. 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