{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T01:51:21Z","timestamp":1790387481809,"version":"4.1.0"},"reference-count":64,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T00:00:00Z","timestamp":1618444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Government of Spain","award":["AGL 2006-1029-C02-01\/ALI"],"award-info":[{"award-number":["AGL 2006-1029-C02-01\/ALI"]}]},{"DOI":"10.13039\/501100012514","name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red-Fisiopatolog\u00eda de la Obesidad y Nutrici\u00f3n","doi-asserted-by":"publisher","award":["CB12\/03\/30002"],"award-info":[{"award-number":["CB12\/03\/30002"]}],"id":[{"id":"10.13039\/501100012514","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Obesity is considered a risk factor for the development of colorectal cancer. In rodents, high-fat (HF) diets are able to increase the formation of azoxymethane (AOM)-induced polyps. Polyphenol-rich apple extracts have antioxidant and anti-inflammatory activities and may induce an amelioration of the manifestations of colorectal cancer. Twenty-seven male Crl:CD-1 mice received AOM during four weeks and were subsequently divided into three groups fed a HF diet (n = 9 each group): a non-supplemented group, a second group supplemented with apple extract at 1%, and a third group supplemented with the same apple extract at 1.5%. Energy metabolism and the respiratory quotient were not affected by the supplementation with the apple extract. Although body weight was not affected by the treatment, the mice supplemented with the apple extract showed less signs of cachexia than the non-treated mice. In the intestine, the mice supplemented with the apple extract showed lower sucrase, dipeptidyl-peptidase IV, and aminopeptidase N activities, and less intestinal lesions (aberrant crypt foci and polyps). Administration of a polyphenol-rich apple extract reduces the number of neoplastic lesions in mice with AOM-induced colorectal cancer and contributes to preserve adipose tissue mass.<\/jats:p>","DOI":"10.3390\/foods10040863","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T12:11:00Z","timestamp":1618488660000},"page":"863","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Azoxymethane-Induced Colorectal Cancer Mice Treated with a Polyphenol-Rich Apple Extract Show Less Neoplastic Lesions and Signs of Cachexia"],"prefix":"10.3390","volume":"10","author":[{"given":"Florencio","family":"Marzo","sequence":"first","affiliation":[{"name":"Laboratory of Animal Physiology and Nutrition, School of Agronomy, Universidad P\u00fablica de Navarra, 31006 Pamplona, Spain"},{"name":"ISFOOD (Research Institute for Innovation &amp; Sustainable Development in Food Chain), Universidad P\u00fablica de Navarra, 31006 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3228-9916","authenticated-orcid":false,"given":"Fermin I.","family":"Milagro","sequence":"additional","affiliation":[{"name":"Center for Nutrition Research, Department of Nutrition, Food Science and Physiology, University of Navarra, 31008 Pamplona, Spain"},{"name":"Navarra Institute for Health Research (IdISNA), 31006 Pamplona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de la Fisiopatolog\u00eda de la Obesidad y Nutrici\u00f3n (CIBERobn), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaione","family":"Barrenetxe","sequence":"additional","affiliation":[{"name":"Center for Nutrition Research, Department of Nutrition, Food Science and Physiology, University of Navarra, 31008 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3258-5966","authenticated-orcid":false,"given":"Mar\u00eda Teresa","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"Departamento de Tecnolog\u00eda de Alimentos, Instituto Nacional de Investigaci\u00f3n Agraria (INIA), Carretera de la Coru\u00f1a, km7, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5218-6941","authenticated-orcid":false,"given":"J. Alfredo","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Center for Nutrition Research, Department of Nutrition, Food Science and Physiology, University of Navarra, 31008 Pamplona, Spain"},{"name":"Navarra Institute for Health Research (IdISNA), 31006 Pamplona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de la Fisiopatolog\u00eda de la Obesidad y Nutrici\u00f3n (CIBERobn), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,15]]},"reference":[{"key":"ref_1","first-page":"33","article-title":"Primary and secondary prevention of colorectal cancer","volume":"7","year":"2014","journal-title":"Clin. Med. Insights Gastroenterol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1016\/j.ejca.2005.06.006","article-title":"How good are rodent models of carcinogenesis in predicting efficacy in humans? A systematic review and meta-analysis of colon chemoprevention in rats, mice and men","volume":"41","author":"Corpet","year":"2005","journal-title":"Eur. J. Cancer."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s11888-010-0046-1","article-title":"Genetic and chemical models of colorectal cancer in mice","volume":"6","author":"Nandan","year":"2010","journal-title":"Curr. Colorectal. Cancer Rep."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Perse, M., and Cerar, A. (2010). Morphological and molecular alterations in 1,2 Dimethylhidrazine and Azoxymethane induced colon carcinogenesis in rats. J. Biochem. Biotech., 2011.","DOI":"10.1155\/2011\/473964"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1207\/S15327914NC431_1","article-title":"Most effective colon cancer chemopreventive agents in rats: A systematic review of aberrant crypt foci and tumor data, ranked by potency","volume":"43","author":"Corpet","year":"2002","journal-title":"Nutr. Cancer"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1016\/j.nutres.2016.10.005","article-title":"Higf-fat Western diet-induced obesity contributes to increased tumor growth in mouse models of human colon cancer","volume":"36","author":"Burrington","year":"2016","journal-title":"Nutr. Res."},{"key":"ref_7","first-page":"106","article-title":"Diet, Lipids and colon cancer","volume":"347","author":"Choi","year":"2019","journal-title":"Int. Rev. Cell Mol. Biol."},{"key":"ref_8","first-page":"79","article-title":"High fat diet-induced obesity increases the formation of colon polyps induced by azoxymethane in mice","volume":"3","author":"Chen","year":"2015","journal-title":"Ann. Transl. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1093\/aje\/kwh319","article-title":"Dietary fat and fatty acids and risk of colorectal cancer in women","volume":"160","author":"Lin","year":"2004","journal-title":"Am. J. Epidemiol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Matusiewicz, M., Baczek, K.B., Kosieradzka, I., Niemiec, T., Grodzik, M., Szczepaniak, J., Orli\u0144ska, S., and W\u0119glarz, Z. (2019). Effect of juice and extracts of saposhnikovia divaricata root on the colon cancer Caco-2 cells. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20184526"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2689","DOI":"10.2147\/IJN.S131973","article-title":"Polyphenol nanoformulations for cancer therapy: Experimentale vidence and clinical perspective","volume":"12","author":"Masoomzadeh","year":"2017","journal-title":"Int. J. Nanomed."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2267","DOI":"10.1093\/carcin\/20.12.2267","article-title":"Effect of vegetable and carotenoid consumption on aberrant crypt multiplicity, a surrogate end-point marker for colorectal cancer in azoxymethane-induced rats","volume":"20","author":"Rijken","year":"1999","journal-title":"Carcinogenesis"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/1477-7827-3-5","article-title":"Red maca (Lepidium meyenii) reduced prostate size in rats","volume":"3","author":"Gonzales","year":"2005","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_14","first-page":"2837","article-title":"Soy extract is more potent than genistein on tumor growth inhibition","volume":"28","author":"Kim","year":"2008","journal-title":"Anticancer Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Niedzwiecki, A., Waheed Roomi, M., Kalinovsky, T., and Matthias, R. (2016). Aticancer effects of polyphenols and their combinations. Nutrients, 8.","DOI":"10.3390\/nu8090552"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"284S","DOI":"10.1093\/ajcn\/81.1.284S","article-title":"Inhibition of carcinogenesis by polyphenols: Evidence from laboratory investigations","volume":"81","author":"Lambert","year":"2005","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1080\/10408398.2016.1231168","article-title":"Absorption, metabolism, anti-cancer effect and molecular targets of epigallocatechin gallate (EGCg): An updated review","volume":"58","author":"Gan","year":"2018","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1002\/mnfr.200800456","article-title":"Formation of hydrogen peroxide in cell culture media by apple polyphenols and its effect on antioxidant biomarkers in the colon cell line HT-29","volume":"53","author":"Bellion","year":"2009","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6459","DOI":"10.1021\/jf200553s","article-title":"Apple peel polyphenols protect against gastrointestinal mucosa alterations induced by indomethacin in rats","volume":"59","author":"Speisky","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.dld.2012.01.009","article-title":"Apple polyphenols extract (APE) improves colon damage in a rat model of colitis","volume":"44","author":"Mazzone","year":"2012","journal-title":"Digest. Liver Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1002\/mnfr.201200122","article-title":"Marie M\u00e9nard apples with high polyphenol content and a low-fat diet reduce 1,2-dimethylhydrazine-induced colon carcinogenesis in rats: Effects on inflammation and apoptosis","volume":"56","author":"Femia","year":"2012","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1016\/j.jada.2011.07.008","article-title":"Fruit and vegetable consumption and the risk of proximal colon, distal colon, and rectal cancers in a case-control study in western Australia","volume":"111","author":"Annema","year":"2011","journal-title":"J. Am. Diet Assoc."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1097\/CEJ.0b013e328333d0cc","article-title":"Case\u2013control study on beneficial effect of regular consumption of apples on colorectal cancer risk in a population with relatively low intake of fruits and vegetables","volume":"19","author":"Jedrychowski","year":"2010","journal-title":"Eur. J. Cancer Prev."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1016\/j.fct.2007.01.003","article-title":"Anti-cancer properties of phenolics from apple waste on colon carcinogenesis in vitro","volume":"45","author":"McCann","year":"2007","journal-title":"Food Chem. Toxicol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1158\/1940-6207.CAPR-10-0359","article-title":"Chemoprevention of intestinal polyps in ApcMin\/+ mice fed with western or balanced diets by drinking annurca apple polyphenol extract","volume":"4","author":"Fini","year":"2011","journal-title":"Cancer Prev. Res."},{"key":"ref_26","unstructured":"Olfert, E., Cross, B., and McWilliam, A. (1993). Guide to the Care and Use of Experimental Animals, Canadian Council on Animal Care. [2nd ed.]."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1113\/jphysiol.1949.sp004363","article-title":"New methods for calculating metabolic rate with special reference to protein metabolism","volume":"109","author":"Weir","year":"1949","journal-title":"J. Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/BF03165765","article-title":"Chronic mild stress induces variations in locomotive behavior and metabolic rates in high fat fed rats","volume":"63","author":"Campion","year":"2007","journal-title":"J. Physiol. Biochem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/0016-5085(90)90288-C","article-title":"Preparation and properties of brush-border membrane vesicles from human small intestine","volume":"98","author":"Davies","year":"1990","journal-title":"Gastroenterology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1079\/BJN20051651","article-title":"Effect of dietary quercetin and sphingomyelin on intestinal nutrient absorption and animal growth","volume":"95","author":"Barrenetxe","year":"2006","journal-title":"Br. J. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0304-3835(95)03788-X","article-title":"Role of aberrant crypt foci in understanding the pathogenesis of colon cancer","volume":"93","author":"Bird","year":"1995","journal-title":"Cancer Lett."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/0304-3835(87)90157-1","article-title":"Observation and quantification of aberrant crypts in the murine colon treated with a colon carcinogen: Preliminary findings","volume":"37","author":"Bird","year":"1987","journal-title":"Cancer Lett."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1158\/0008-5472.121.65.1","article-title":"Flat dysplastic aberrant crypt foci are related to tumorigenesis in the colon of azoxymethane-treated rat","volume":"65","author":"Paulsen","year":"2005","journal-title":"Cancer Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1021\/jf00084a019","article-title":"Rapid method for determination of total fatty acid content and composition of feedstuffs and feces","volume":"36","author":"Sukhija","year":"1988","journal-title":"J. Agric. Food Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1016\/j.meatsci.2012.06.013","article-title":"Beef, chicken and lamb fatty acid analysis\u2014A simplified direct bimethylation procedure using freeze-dried material","volume":"92","author":"Lee","year":"2012","journal-title":"Meat Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1002\/(SICI)1097-0010(19990301)79:3<362::AID-JSFA256>3.0.CO;2-D","article-title":"Chlorogenic acids and other cinnamates\u2014Nature, occurrence and dietary burden","volume":"79","author":"Clifford","year":"1999","journal-title":"J. Sci. Food Agric."},{"key":"ref_38","first-page":"1564","article-title":"Aberrant crypts: Putative preneoplastic foci in human colonic mucosa","volume":"51","author":"Pretlow","year":"1991","journal-title":"Cancer Res."},{"key":"ref_39","first-page":"57","article-title":"Classification of aberrant crypt foci and microadenomas in human colon","volume":"1","author":"Roncucci","year":"1991","journal-title":"Cancer Epidemiol. Biomark. Prev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1093\/carcin\/18.3.465","article-title":"Histomorphological and immunohistochemical characterization of colonic aberrant crypt foci in rats: Relationship to growth factor expression","volume":"18","author":"Thorup","year":"1997","journal-title":"Carcinogenesis"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1002\/ijc.24417","article-title":"Mucin-depleted foci show strong activation of inflammatory markers in 1,2-dimethylhydrazine-induced carcinogenesis and are promoted by the inflammatory agent sodium dextran sulfate","volume":"125","author":"Femia","year":"2009","journal-title":"Int. J. Cancer"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1016\/j.biopha.2008.06.031","article-title":"Protective role of luteolin on the status of lipid peroxidation and antioxidant defense against azoxymethane-induced experimental colon carcinogenesis","volume":"62","author":"Ashokkumar","year":"2008","journal-title":"Biomed Pharmacother."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1002\/mnfr.201000412","article-title":"Molecular mechanisms for chemoprevention of colorectal cancer by natural dietary compounds","volume":"55","author":"Pan","year":"2011","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.canlet.2017.11.002","article-title":"Oxidative stress and dietary phytochemicals; role in cancer chemoprevention and treatment","volume":"413","author":"Chikara","year":"2018","journal-title":"Cancer Lett."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/0026-0495(88)90110-2","article-title":"The theoretical bases of indirect calorimetry: A review","volume":"37","author":"Ferrannini","year":"1988","journal-title":"Metabolism"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.metabol.2008.03.003","article-title":"Quercetin transiently increases energy expenditure but persistently decreases circulating markers of inflammation in C57BL\/6J mice fed a high-fat diet","volume":"57","author":"Stewart","year":"2008","journal-title":"Metabolism"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1038\/sj.ijo.0802926","article-title":"Epigallocatechin gallate attenuates diet-induced obesity in mice by decreasing energy absorption and increasing fat oxidation","volume":"29","author":"Klaus","year":"2005","journal-title":"Int. J. Obes. Relat. Metab. Disord."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1002\/mnfr.200400084","article-title":"Quercetin alleviates activities of intestinal and renal disaccharidases in streptozotocin-induced diabetic rats","volume":"49","author":"Ramachandra","year":"2005","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1093\/jn\/120.4.346","article-title":"Effects of foods rich in polyphenols on nitrogen excretion in rats","volume":"120","author":"Shahkhalili","year":"1990","journal-title":"J. Nutr."},{"key":"ref_50","first-page":"3800","article-title":"Nitrogen excretion in cancer cachexia and its modification by a high fat diet in mice","volume":"49","author":"Beck","year":"1989","journal-title":"Cancer Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1038\/bjc.1987.149","article-title":"Reduction of weight loss and tumour size in a cachexia model by a high fat diet","volume":"56","author":"Tisdale","year":"1987","journal-title":"Br. J. Cancer"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1866","DOI":"10.1007\/s10620-012-2092-9","article-title":"High-fat diet alters gene expression in the liver and colon: Links to increased development of aberrant crypt foci","volume":"57","author":"Padidar","year":"2012","journal-title":"Dig. Dis. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1021\/jf063078t","article-title":"Cloudy apple juice is more effective than apple polyphenols and an apple juice derived cloud fraction in a rat model of colon carcinogenesis","volume":"55","author":"Barth","year":"2007","journal-title":"J. Agric. Food Chem."},{"key":"ref_54","first-page":"163","article-title":"Inhibitory effects of chlorogenic acid on azoxymethane-induced colon carcinogenesis in male F344 rats","volume":"3","author":"Matsunaga","year":"2002","journal-title":"Asian Pac. J. Cancer Prev."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"223S","DOI":"10.1093\/jn\/137.1.223S","article-title":"Dietary polyphenols may affect dna methylation","volume":"137","author":"Fang","year":"2007","journal-title":"J. Nutr."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1093\/carcin\/bgm204","article-title":"Green tea polyphenols inhibit colorectal aberrant crypt foci (ACF) formation and prevent oncogenic changes in dysplastic ACF in azoxymethane-treated F344 rats","volume":"29","author":"Xiao","year":"2008","journal-title":"Carcinogenesis"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1080\/01635580701365050","article-title":"Inhibition of intestinal tumorigenesis in ApcMin\/+ mice by green tea polyphenols (polyphenon e) and individual catechins","volume":"59","author":"Hao","year":"2007","journal-title":"Nutr. Cancer"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Tan, C.W., Hirokawa, Y., Gardiner, B.S., Smith, D.W., and Burgess, A.W. (2013). Colon cryptogenesis: Asymmetric budding. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0078519"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1002\/biof.1185","article-title":"Green tea polyphenol EGCG suppresses Wnt\/\u03b2-catenin signaling by promoting GSK-3\u03b2- and PP2A-independent \u03b2-catenin phosphorylation\/degradation","volume":"40","author":"Oh","year":"2014","journal-title":"BioFactors"},{"key":"ref_60","first-page":"69","article-title":"EGCG inhibits activation of HER3 and expression of cyclooxygenase-2 in human colon cancer cells","volume":"5","author":"Shimizu","year":"2005","journal-title":"J. Exp. Ther. Oncol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1002\/1097-0142(20010901)92:5<1331::AID-CNCR1455>3.0.CO;2-M","article-title":"Epidermal growth factor receptor immunohistochemical reactivity in patients with American Joint Committee on Cancer Stage IV colon adenocarcinoma: Implications for a standardized scoring system","volume":"92","author":"Goldstein","year":"2001","journal-title":"Cancer"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1038\/oby.2009.326","article-title":"Comparison of lipid and fatty acid composition of the liver, subcutaneous and intra-abdominal adipose tissue, and serum","volume":"18","author":"Kotronen","year":"2010","journal-title":"Obesity"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1079\/BJN2002727","article-title":"Effects of dietary fat source and breed on the carcass composition, n\u22123 polyunsaturated fatty acid and conjugated linoleic acid content of sheep meat and adipose tissue","volume":"88","author":"Wachira","year":"2002","journal-title":"Br. J. Nutr."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"5018","DOI":"10.3390\/nu6115018","article-title":"The influence of dietary fat on liver fat accumulation","volume":"6","author":"Green","year":"2014","journal-title":"Nutrients"}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/10\/4\/863\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:48:27Z","timestamp":1760161707000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/10\/4\/863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,15]]},"references-count":64,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,4]]}},"alternative-id":["foods10040863"],"URL":"https:\/\/doi.org\/10.3390\/foods10040863","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,15]]}}}