{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T20:40:28Z","timestamp":1782420028055,"version":"3.54.5"},"reference-count":29,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T00:00:00Z","timestamp":1621382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["RF-2016-02364002"],"award-info":[{"award-number":["RF-2016-02364002"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Salmonella and Campylobacter ssp. are bacterial pathogens responsible for most foodborne infections in EU countries. Poultry serves as a reservoir for these pathogens, and its important role in the meat industry makes it essential to develop a rapid detection assay able to provide results in one day. Indeed, the rapid identification of foodborne pathogens is an important instrument for the monitoring and prevention of epidemic outbreaks. To date, Salmonella and Campylobacter screening is mainly conducted through molecular methods (PCR or real-time PCR) performed after 18\u201324 h long enrichments. In this study, we evaluated short enrichments (0, 2, 4, and 6 h) combined with a colorimetric loop-mediated isothermal AMPlification (LAMP) or real-time PCR to detect Salmonella and Campylobacter in poultry meat contaminated at different concentration levels (101, 103, and 105 CFU\/g). Our results show that real-time PCR allows the detection of Salmonella and Campylobacter, even after shorter enrichment times than prescribed by ISO references; particularly, it detected Salmonella down to 101 CFU\/g since T0 and Campylobacter from 103 CFU\/g since T0. Detection with LAMP was comparable to real-time PCR without the requirement of a thermal cycler and with shorter execution times. These characteristics make colorimetric LAMP a valid alternative when one-day results are needed, improving the timely identification of positive meat batches, even in the absence of specialized instrumentation.<\/jats:p>","DOI":"10.3390\/foods10051132","type":"journal-article","created":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T12:58:07Z","timestamp":1621429087000},"page":"1132","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["One-Day Molecular Detection of Salmonella and Campylobacter in Chicken Meat: A Pilot Study"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3626-0360","authenticated-orcid":false,"given":"Andrea","family":"Zendrini","sequence":"first","affiliation":[{"name":"Department of Animal Science, Food and Nutrition\u2014DIANA, Universit\u00e0 Cattolica del Sacro Cuore, Via E. Parmense, 84, 29122 Piacenza, Italy"},{"name":"Department of Molecular and Translational Medicine, University of Brescia, Viale Europa, 11, 25123 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Carta","sequence":"additional","affiliation":[{"name":"Department of Food Safety, Istituto Zooprofilattico della Lombardia e dell\u2019Emilia Romagna, Via A. Bianchi, 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4569-5793","authenticated-orcid":false,"given":"Virginia","family":"Filipello","sequence":"additional","affiliation":[{"name":"Department of Food Safety, Istituto Zooprofilattico della Lombardia e dell\u2019Emilia Romagna, Via A. Bianchi, 9, 25124 Brescia, Italy"},{"name":"National Reference Centre for Emerging Risks in Food Safety\u2014CRESA, Istituto Zooprofilattico della Lombardia e dell\u2019Emilia Romagna, Via G. Celoria, 12, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Ragni","sequence":"additional","affiliation":[{"name":"Department of Food Safety, Istituto Zooprofilattico della Lombardia e dell\u2019Emilia Romagna, Via A. Bianchi, 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Cosciani-Cunico","sequence":"additional","affiliation":[{"name":"Department of Food Safety, Istituto Zooprofilattico della Lombardia e dell\u2019Emilia Romagna, Via A. Bianchi, 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1666-7518","authenticated-orcid":false,"given":"Sara","family":"Arnaboldi","sequence":"additional","affiliation":[{"name":"Department of Food Safety, Istituto Zooprofilattico della Lombardia e dell\u2019Emilia Romagna, Via A. Bianchi, 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1875-4305","authenticated-orcid":false,"given":"Barbara","family":"Bertasi","sequence":"additional","affiliation":[{"name":"Department of Food Safety, Istituto Zooprofilattico della Lombardia e dell\u2019Emilia Romagna, Via A. Bianchi, 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niccol\u00f2","family":"Franceschi","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Food and Nutrition\u2014DIANA, Universit\u00e0 Cattolica del Sacro Cuore, Via E. Parmense, 84, 29122 Piacenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Ajmone-Marsan","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Food and Nutrition\u2014DIANA, Universit\u00e0 Cattolica del Sacro Cuore, Via E. Parmense, 84, 29122 Piacenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6917-8478","authenticated-orcid":false,"given":"Dario","family":"De Medici","sequence":"additional","affiliation":[{"name":"Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanit\u00e0, Viale Regina Elena, 299, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina Nadia","family":"Losio","sequence":"additional","affiliation":[{"name":"Department of Food Safety, Istituto Zooprofilattico della Lombardia e dell\u2019Emilia Romagna, Via A. Bianchi, 9, 25124 Brescia, Italy"},{"name":"National Reference Centre for Emerging Risks in Food Safety\u2014CRESA, Istituto Zooprofilattico della Lombardia e dell\u2019Emilia Romagna, Via G. Celoria, 12, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"EFSA, and ECDC (2021). The European Union One Health 2019 Zoonoses Report. EFSA J., 19, e06406. Available online: https:\/\/efsa.onlinelibrary.wiley.com\/doi\/abs\/10.2903\/j.efsa.2021.6406.","DOI":"10.2903\/j.efsa.2021.6406"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.cmi.2015.12.004","article-title":"Salmonellosis: The role of poultry meat","volume":"22","author":"Antunes","year":"2016","journal-title":"Clin. Microbiol. 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