{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T04:25:59Z","timestamp":1760502359821,"version":"build-2065373602"},"reference-count":25,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,7]],"date-time":"2022-01-07T00:00:00Z","timestamp":1641513600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100019285","name":"Bio-Based Industries Joint Undertaking","doi-asserted-by":"publisher","award":["792054"],"award-info":[{"award-number":["792054"]}],"id":[{"id":"10.13039\/501100019285","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>The aim of this work was to develop innovative and sustainable extraction, concentration, and purification technologies aimed to recover target substances from corn oil, obtained as side stream product of biomass refineries. Residues of bioactive compounds such as carotenoids, phytosterols, tocopherols, and polyphenols could be extracted from this matrix and applied as ingredients for food and feeds, nutraceuticals, pharmaceuticals, and cosmetic products. These molecules are well known for their antioxidant and antiradical capacity, besides other specific biological activities, generically involved in the prevention of chronic and degenerative diseases. The project involved the development of methods for the selective extraction of these minor components, using as suitable extraction technique solid phase extraction. All the extracted and purified fractions were evaluated by NMR spectroscopic analyses and UV\u2013Vis spectrophotometric techniques and characterized by quali-quantitative HPLC analyses. TPC (total phenolic content) and TFC (total flavonoid content) were also determined. DPPH and ABTS radical were used to evaluate radical quenching abilities. Acetylcholinesterase (AChE), amylase, glucosidase, and tyrosinase were selected as enzymes in the enzyme inhibitory assays. The obtained results showed the presence of a complex group of interesting molecules with strong potential in market applications according to circular economy principles.<\/jats:p>","DOI":"10.3390\/foods11020153","type":"journal-article","created":{"date-parts":[[2022,1,9]],"date-time":"2022-01-09T20:21:56Z","timestamp":1641759716000},"page":"153","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Valorization of By-Products from Biofuel Biorefineries: Extraction and Purification of Bioactive Molecules from Post-Fermentation Corn Oil"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9618-1410","authenticated-orcid":false,"given":"Francesco","family":"Cairone","sequence":"first","affiliation":[{"name":"Department of Drug Chemistry and Technology, Sapienza, University of Roma, P.le A. Moro 5, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8649-0017","authenticated-orcid":false,"given":"Stefania","family":"Cesa","sequence":"additional","affiliation":[{"name":"Department of Drug Chemistry and Technology, Sapienza, University of Roma, P.le A. Moro 5, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7538-2424","authenticated-orcid":false,"given":"Alessia","family":"Ciogli","sequence":"additional","affiliation":[{"name":"Department of Drug Chemistry and Technology, Sapienza, University of Roma, P.le A. Moro 5, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giancarlo","family":"Fabrizi","sequence":"additional","affiliation":[{"name":"Department of Drug Chemistry and Technology, Sapienza, University of Roma, P.le A. Moro 5, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonella","family":"Goggiamani","sequence":"additional","affiliation":[{"name":"Department of Drug Chemistry and Technology, Sapienza, University of Roma, P.le A. Moro 5, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7792-774X","authenticated-orcid":false,"given":"Antonia","family":"Iazzetti","sequence":"additional","affiliation":[{"name":"Department of Drug Chemistry and Technology, Sapienza, University of Roma, P.le A. Moro 5, 00185 Rome, Italy"},{"name":"Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Universit\u00e0 Cattolica del Sacro Cuore, L.go Francesco Vito 1, 00168 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5325-2189","authenticated-orcid":false,"given":"Gabriella","family":"Di Lena","sequence":"additional","affiliation":[{"name":"CREA Research Centre for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3728-265X","authenticated-orcid":false,"given":"Jose","family":"Sanchez del Pulgar","sequence":"additional","affiliation":[{"name":"CREA Research Centre for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Lucarini","sequence":"additional","affiliation":[{"name":"CREA Research Centre for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luca","family":"Cant\u00f2","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University \u201cG. d\u2019Annunzio\u201d of Chieti-Pescara, 66100 Chieti, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6548-7823","authenticated-orcid":false,"given":"Gokhan","family":"Zengin","sequence":"additional","affiliation":[{"name":"Department of Biology, Science Faculty, Selcuk University, 42130 Konya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Ondrej\u00ed\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"ENVIRAL a.s., Trnavsk\u00e1 Cesta, 920 41 Leopoldov, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,7]]},"reference":[{"key":"ref_1","unstructured":"European Commission (2020). Communication from the Commission to the European Parliament, the Council, the European Economic and Social Committee and the Committee of the Regions. A New Circular Economy Action Plan for a Cleaner and More Competitive Europe, European Commission."},{"key":"ref_2","first-page":"209","article-title":"Utilization of plant by-products for the recovery of proteins, dietary fibers, antioxidants, and colorants","volume":"Volume 3","author":"Oreopoulou","year":"2007","journal-title":"Utilization of By-Products and Treatment of Waste in the Food Industry"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jclepro.2013.10.051","article-title":"Current options for the valorization of food manufacturing waste: A review","volume":"65","author":"Mirabella","year":"2014","journal-title":"J. Clean. Prod."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ben-Othman, S., J\u00f5udu, I., and Bhat, R. (2020). Bioactives from agri-food wastes: Present insights and future challenges. Molecules, 25.","DOI":"10.3390\/molecules25030510"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.psep.2011.06.010","article-title":"A summary of the available technologies for biodiesel production based on a comparison of different feedstock\u2019s properties","volume":"90","author":"Marchetti","year":"2012","journal-title":"Process Saf. Environ. Prot."},{"key":"ref_6","unstructured":"ePURE (2021, November 22). European Renewable Ethanol\u2014Key Figures. Available online: https:\/\/www.google.com.hk\/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&ved=2ahUKEwj77ou2g5_1AhUUT2wGHViIAmYQFnoECAoQAQ&url=https%3A%2F%2Fwww.epure.org%2Fwp-content%2Fuploads%2F2021%2F09%2F210823-DEF-PR-European-renewable-ethanol-Key-figures-2020-web.pdf&usg=AOvVaw1upJjrrr-43EX96SRZinr1."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Di Lena, G., Ondrej\u00ed\u010dkov\u00e1, P., del Pulgar, J.S., Cyprichov\u00e1, V., Je\u017eovi\u010d, T., Lucarini, M., Lombardi Boccia, G., Ferrari Nicoli, S., Gabrielli, P., and Aguzzi, A. (2020). Towards a Valorization of Corn Bioethanol Side Streams: Chemical Characterization of Post Fermentation Corn Oil and Thin Stillage. Molecules, 25.","DOI":"10.3390\/molecules25153549"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Di Lena, G., Sanchez del Pulgar, J., Lombardi Boccia, G., Casini, I., and Ferrari Nicoli, S. (2020). Corn Bioethanol Side Streams: A Potential Sustainable Source of Fat-Soluble Bioactive Molecules for High-Value Applications. Foods, 9.","DOI":"10.3390\/foods9121788"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"26991","DOI":"10.1039\/C5RA03963K","article-title":"Characterization of phenolic compounds and antioxidant properties of Glycyrrhiza glabra L. rhizomes and roots","volume":"5","author":"Martins","year":"2015","journal-title":"RSC Adv."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.jff.2016.05.007","article-title":"Determination of lignans and phenolic components of Schisandra chinensis (Turcz.) Baill. using HPLC-ESI-ToF-MS and HPLC-online TEAC: Contribution of individual components to overall antioxidant activity and comparison with traditional antioxidant assays","volume":"24","author":"Mocan","year":"2016","journal-title":"J. Funct. Foods"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.indcrop.2015.12.033","article-title":"A study on in vitro enzyme inhibitory properties of Asphodeline anatolica: New sources of natural inhibitors for public health problems","volume":"83","author":"Zengin","year":"2016","journal-title":"Ind. Crop. Prod."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Carradori, S., Cairone, F., Garzoli, S., Fabrizi, G., Iazzetti, A., Giusti, A.M., Menghini, L., Uysal, S., Ak, G., and Zengin, G. (2020). Phytocomplex Characterization and Biological Evaluation of Powdered Fruits and Leaves from Elaeagnus angustifolia. Molecules, 25.","DOI":"10.3390\/molecules25092021"},{"key":"ref_13","first-page":"531","article-title":"Crocus sativus, Serenoa repens and Pinus massoniana extracts modulate inflammatory response in isolated rat prostate challenged with LPS","volume":"31","author":"Chiavaroli","year":"2017","journal-title":"J. Biol. Regul. Homeost. Agents"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.trac.2018.08.009","article-title":"Review of nanomaterials as sorbents in solid-phase extraction for environmental samples","volume":"108","author":"Azzouz","year":"2018","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1007\/s13197-010-0190-2","article-title":"The effect of consecutive steps of refining on squalene content of vegetable oils","volume":"48","author":"Nergiz","year":"2011","journal-title":"J. Food Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"540","DOI":"10.3390\/molecules14010540","article-title":"Biological and Pharmacological Activities of Squalene and Related Compounds: Potential Uses in Cosmetic Dermatology","volume":"14","author":"Huang","year":"2009","journal-title":"Molecules"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.foodchem.2015.02.130","article-title":"Antioxidant activity of phenolic compounds added to a functional emulsion containing omega-3 fatty acids and plant sterol esters","volume":"182","author":"Espinosa","year":"2015","journal-title":"Food Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.chemphyslip.2011.06.005","article-title":"Current and new insights on phytosterol oxides in plant sterol-enriched food","volume":"164","year":"2011","journal-title":"Chem. Phys. Lipids"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3716","DOI":"10.1007\/s12161-017-0927-x","article-title":"Simultaneous analysis of tocopherols, phytosterols, and squalene in vegetable oils by high-performance liquid chromatography","volume":"10","author":"Yuan","year":"2017","journal-title":"Food Anal. Methods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1002\/(SICI)1438-9312(200001)102:1<37::AID-EJLT37>3.0.CO;2-1","article-title":"Cholesterol-lowering effect of plant sterols","volume":"102","author":"Wester","year":"2000","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1021\/jf981029d","article-title":"Quantification of Carotenoid and Tocopherol Antioxidants in Zea mays","volume":"47","author":"Kurilich","year":"1999","journal-title":"J. Agric. Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1007\/s11746-007-1137-2","article-title":"A comparison of the levels of lutein and zeaxanthin in corn germ oil, corn fiber oil and corn kernel oil","volume":"84","author":"Moreau","year":"2007","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.fct.2018.01.054","article-title":"UHPLC-QTOF-MS analysis of bioactive constituents from two Romanian Goji (Lycium barbarum L.) berries cultivars and their antioxidant, enzyme inhibitory, and real-time cytotoxicological evaluation","volume":"115","author":"Mocan","year":"2018","journal-title":"Food Chem. Toxicol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1900079","DOI":"10.1002\/ejlt.201900079","article-title":"Evaluation of the antioxidant properties of micronutrients in different vegetable oils","volume":"122","author":"Liu","year":"2020","journal-title":"Eur. J. Lipid Sci. Technol"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1002\/jsfa.10722","article-title":"Antioxidant quantification in different portions obtained during olive oil extraction process in an olive oil press mill","volume":"101","author":"Romeo","year":"2021","journal-title":"J. Sci. Food Agric."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/2\/153\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:38:09Z","timestamp":1760362689000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/2\/153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,7]]},"references-count":25,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,1]]}},"alternative-id":["foods11020153"],"URL":"https:\/\/doi.org\/10.3390\/foods11020153","relation":{},"ISSN":["2304-8158"],"issn-type":[{"type":"electronic","value":"2304-8158"}],"subject":[],"published":{"date-parts":[[2022,1,7]]}}}