{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:28:56Z","timestamp":1791228536915,"version":"4.1.0"},"reference-count":52,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,2,7]],"date-time":"2022-02-07T00:00:00Z","timestamp":1644192000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NUTR-AGE","award":["FOE-2019, DSB.AD004.271"],"award-info":[{"award-number":["FOE-2019, DSB.AD004.271"]}]},{"name":"SOILLESS GO","award":["B97H20000990009"],"award-info":[{"award-number":["B97H20000990009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Zinc (Zn) is an essential key nutrient in different biochemical and physiological processes. The nutritional deficit of this mineral element is estimated to affect the health of over 3 billion people worldwide. Several strategies are available to reduce the negative impact of mineral malnutrition; among them, biofortification is the practice of deliberately increasing the nutrients and healthy compounds in the edible parts of vegetables. This study aims to evaluate Zn bioaccessibility in biofortified and non-biofortified rocket and purslane using an in vitro gastrointestinal digestion process and measure the concentration of other mineral elements (Al, B, Ca, Fe, K, Mg, Mn, and Sr) released during the digestion process from rocket and purslane biofortified with Zn. The bioaccessible Zn in biofortified rocket and purslane ranged from 7.43 to 16.91 mg\/kg, respectively. In addition, the daily intake, the RDA coverage (%), and the hazard quotient (HQ) for the intake of Zn (resulting from the consumption of 100 g of rocket and purslane) were calculated. The calculated HQ highlights the safety of these baby leaf vegetables. The study confirms that it is possible to obtain Zn-biofortified rocket and purslane with high Zn bioaccessibility by adopting an appropriate mineral plant nutrition solution enriched in Zn.<\/jats:p>","DOI":"10.3390\/foods11030484","type":"journal-article","created":{"date-parts":[[2022,2,7]],"date-time":"2022-02-07T20:36:42Z","timestamp":1644266202000},"page":"484","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Mineral Composition and Bioaccessibility in Rocket and Purslane after Zn Biofortification Process"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7011-9347","authenticated-orcid":false,"given":"Massimiliano","family":"D\u2019Imperio","sequence":"first","affiliation":[{"name":"Institute of Sciences of Food Production, CNR\u2014National Research Council of Italy, Via Amendola 122\/D, 70126 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3074-0010","authenticated-orcid":false,"given":"Francesco Fabiano","family":"Montesano","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production, CNR\u2014National Research Council of Italy, Via Amendola 122\/D, 70126 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0015-7238","authenticated-orcid":false,"given":"Francesco","family":"Serio","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production, CNR\u2014National Research Council of Italy, Via Amendola 122\/D, 70126 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Santovito","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production, CNR\u2014National Research Council of Italy, Via Amendola 122\/D, 70126 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo","family":"Parente","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production, CNR\u2014National Research Council of Italy, Via Amendola 122\/D, 70126 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1021\/pr050361j","article-title":"Counting the zinc-proteins encoded in the human genome","volume":"5","author":"Andreini","year":"2006","journal-title":"J. Proteome Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3213","DOI":"10.1007\/s12011-020-02462-8","article-title":"Trace Element Zinc, a Nature\u2019s Gift to Fight Unprecedented Global Pandemic COVID-19","volume":"199","author":"Sharma","year":"2020","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.jfca.2005.10.004","article-title":"Trace elements in foods: Zinc contents of raw foods\u2014a comparison of data originating from different geographical regions of the world","volume":"19","author":"Scherz","year":"2006","journal-title":"J. Food Comp. Anal."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"126621","DOI":"10.1016\/j.foodchem.2020.126621","article-title":"Phytochemical composition and nutritional value of different plant parts in two cultivated and wild purslane (Portulaca oleracea L.) genotypes","volume":"320","author":"Nemzer","year":"2020","journal-title":"Food Chem."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"De Almeida, H.J., Vergara Carmona, V.M., Ferreira Inoc\u00eancio, M., Furtini Neto, A.E., Cec\u00edlio Filho, A.B., and Mauad, M. (2020). Soil Type and Zinc Doses in Agronomic Biofortification of Lettuce Genotypes. Agronomy, 10.","DOI":"10.3390\/agronomy10010124"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Khoja, K., Buckley, A.F., Aslam, M., Sharp, A.P., and Latunde-Dada, G.O. (2020). In vitro bioaccessibility and bioavailability of iron from mature and microgreen fenugreek, rocket and broccoli. Nutrients, 12.","DOI":"10.3390\/nu12041057"},{"key":"ref_7","unstructured":"(2021, November 10). National Institute of Health (NIH), Available online: https:\/\/ods.od.nih.gov\/factsheets\/Zinc-HealthProfessional\/#h2."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Wessells, K.R., and Brown, K.H. (2012). Estimating the global prevalence of zinc deficiency: Results based on zinc availability in national food supplies and the prevalence of stunting. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0050568"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"10974","DOI":"10.1038\/srep10974","article-title":"Dietary calcium and zinc deficiency risks are decreasing but remain prevalent","volume":"5","author":"Kumssa","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_10","first-page":"CD010697","article-title":"Fortification of staple foods with zinc for improving zinc status and other health outcomes in the general population","volume":"6","author":"Shah","year":"2016","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_11","unstructured":"International Zinc Nutrition Consultative Group (2021, November 02). BIZIFED2. Available online: https:\/\/www.izincg.org\/."},{"key":"ref_12","unstructured":"(2021, October 29). VIRCA Plus. Available online: https:\/\/cassavaplus.org\/."},{"key":"ref_13","unstructured":"(2021, October 29). Harvest Plus. Available online: https:\/\/www.harvestplus.org\/."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1016\/j.foodres.2012.12.021","article-title":"Producing more with less: Strategies and novel technologies for plant-based food biofortification","volume":"54","author":"Carvalho","year":"2013","journal-title":"Food Res. Int"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5203","DOI":"10.1038\/s41467-020-19020-4","article-title":"Multiplying the efficiency and impact of biofortification through metabolic engineering","volume":"11","author":"Bhullar","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Buturi, C.V., Mauro, R.P., Fogliano, V., Leonardi, C., and Giuffrida, F. (2021). Mineral Biofortification of Vegetables as a Tool to Improve Human Diet. Foods, 10.","DOI":"10.3390\/foods10020223"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"31662","DOI":"10.1038\/srep31662","article-title":"Green bean biofortification for Si through soilless cultivation: Plant response and Si bioaccessibility in pods","volume":"6","author":"Montesano","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.3389\/fpls.2018.01254","article-title":"Enhancing quality of fresh vegetables through salinity eustress and biofortification applications facilitated by soilless cultivation","volume":"9","author":"Rouphael","year":"2018","journal-title":"Front. Plant Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Gonnella, M., Renna, M., D\u2019Imperio, M., Santamaria, P., and Serio, F. (2019). Iodine biofortification of four brassica genotypes is effective already at low rates of potassium iodate. Nutrients, 11.","DOI":"10.3390\/nu11020451"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4319","DOI":"10.1021\/jf0602572","article-title":"Changes in antioxidant content of tomato fruits in response to cultivar and nutrient solution composition","volume":"54","author":"Fanasca","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1469-8137.2008.02738.x","article-title":"Biofortification of crops with seven mineral elements often lacking in human diets\u2013iron, zinc, copper, calcium, magnesium, selenium and iodine","volume":"182","author":"White","year":"2009","journal-title":"New Phytol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.scienta.2014.03.051","article-title":"Characterization of purslane (Portulaca oleracea L.) accessions: Suitability as ready-to-eat product","volume":"172","author":"Parra","year":"2014","journal-title":"Sci. Hortic."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.1002\/(SICI)1097-0010(199910)79:13<1882::AID-JSFA450>3.0.CO;2-D","article-title":"A survey of nitrate and oxalate content in fresh vegetables","volume":"79","author":"Santamaria","year":"1999","journal-title":"J. Sci. Food Agric."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5330","DOI":"10.1021\/jf501546a","article-title":"Pilot study of diet and microbiota: Interactive associations of fibers and polyphenols with human intestinal bacteria","volume":"62","author":"Cuervo","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4651","DOI":"10.3390\/molecules18044651","article-title":"Evaluation of biological value and appraisal of polyphenols and glucosinolates from organic baby-leaf salads as antioxidants and antimicrobials against important human pathogenic bacteria","volume":"18","author":"Aires","year":"2013","journal-title":"Molecules"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1007\/s00394-016-1332-8","article-title":"Bioactive compounds from regular diet and faecal microbial metabolites","volume":"57","author":"Salazar","year":"2018","journal-title":"Eur. J. Nutr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"S181","DOI":"10.1079\/BJN2000312","article-title":"Micronutrient interactions: Effects on absorption and bioavailability","volume":"85","year":"2001","journal-title":"Br. J. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.foodhyd.2016.07.030","article-title":"Pectin nanostructure influences pectin-cation interactions and in vitro-bioaccessibility of Ca2+, Zn2+, Fe2+ and Mg2+-ions in model systems","volume":"62","author":"Kyomugasho","year":"2017","journal-title":"Food Hydrocoll."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1039\/C5FO00137D","article-title":"Polyphenols from artichoke heads (Cynara cardunculus (L.) subsp. scolymus Hayek): In vitro bio-accessibility, intestinal uptake and bioavailability","volume":"6","author":"Garbetta","year":"2015","journal-title":"Food Funct."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1016\/j.foodchem.2006.07.015","article-title":"Zinc and iron contents and their bioaccessibility in cereals and pulses consumed in India","volume":"102","author":"Hemalatha","year":"2007","journal-title":"Food Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.foodchem.2004.08.009","article-title":"Bioaccessibility of minerals in school meals: Comparison between dialysis and solubility methods","volume":"92","author":"Camara","year":"2005","journal-title":"Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.foodchem.2016.06.071","article-title":"Calcium biofortification and bioaccessibility in soilless \u201cbaby leaf\u201d vegetable production","volume":"213","author":"Renna","year":"2016","journal-title":"Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Di Gioia, F., Renna, M., and Santamaria, P. (2017). Sprouts, Microgreens and \u201cBaby Leaf\u201d Vegetables. Minimally Processed Refrigerated Fruits and Vegetables, Springer.","DOI":"10.1007\/978-1-4939-7018-6_11"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"580596","DOI":"10.3389\/fpls.2021.580596","article-title":"Posidonia natural residues as growing substrate component: An ecofriendly method to improve nutritional profile of brassica microgreens","volume":"12","author":"Montesano","year":"2021","journal-title":"Front. Plant Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2082","DOI":"10.1021\/jf000775r","article-title":"Assessment of degradation and intestinal cell uptake of carotenoids and chlorophyll derivatives from spinach puree using an in vitro digestion and Caco-2 human cell model","volume":"49","author":"Ferruzzi","year":"2001","journal-title":"J. Agric. Food Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.microc.2015.06.001","article-title":"Bioaccessibility performance data for fifty-seven elements in guidance materials BGS 102","volume":"123","author":"Hamilton","year":"2015","journal-title":"Microchem. J."},{"key":"ref_37","unstructured":"Integrated Risk Information System (Iris) (2011). Integrated Risk Information System\u2014Database 2011, United States Environmental Protection Agency."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"B\u0105czek-Kwinta, R., Baran, A., Simlat, M., Lang, J., Bieniek, M., and Florek, B. (2020). Enrichment of Different Plant Seeds with Zinc and Assessment of Health Risk of Zn-Fortified Sprouts Consumption. Agronomy, 10.","DOI":"10.3390\/agronomy10070937"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.jtemb.2018.02.009","article-title":"Zinc in soils, water and food crops","volume":"49","author":"Noulas","year":"2018","journal-title":"J. Trace Elem. Med. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.plantsci.2017.02.004","article-title":"Zinc biofortification improves phytochemicals and amino-acidic profile in Brassica oleracea cv. Bronco","volume":"258","author":"Blasco","year":"2017","journal-title":"Plant Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1002\/jsfa.7983","article-title":"Zn-biofortification enhanced nitrogen metabolism and photorespiration process in green leafy vegetable Lactuca sativa L.","volume":"97","author":"Lentini","year":"2017","journal-title":"J. Sci. Food Agric."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.1002\/jsfa.6658","article-title":"Biofortification of soybean sprouts with zinc and bioaccessibility of zinc in the sprouts","volume":"94","author":"Zou","year":"2014","journal-title":"J. Sci. Food Agric."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Di Gioia, F., Petropoulos, S.A., Ozores-Hampton, M., Morgan, K., and Rosskopf, E.N. (2019). Zinc and Iron Agronomic Biofortification of Brassicaceae Microgreens. Agronomy, 9.","DOI":"10.3390\/agronomy9110677"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1080\/00103624.2017.1407429","article-title":"Effects of nutrient antagonism and synergism on yield and fertilizer use efficiency","volume":"48","author":"Rietra","year":"2017","journal-title":"Commun. Soil Sci. Plant Anal."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"D\u2019Imperio, M., Montesano, F.F., Renna, M., Parente, A., Logrieco, A.F., and Serio, F. (2019). Hydroponic Production of Reduced-Potassium Swiss Chard and Spinach: A Feasible Agronomic Approach to Tailoring Vegetables for Chronic Kidney Disease Patients. Agronomy, 9.","DOI":"10.3390\/agronomy9100627"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1080\/10408398.2018.1552243","article-title":"Barriers impairing mineral bioaccessibility and bioavailability in plant-based foods and the perspectives for food processing","volume":"60","author":"Rousseau","year":"2020","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1002\/jsfa.7142","article-title":"Silicon biofortification of leafy vegetables and its bioaccessibility in the edible parts","volume":"96","author":"Renna","year":"2016","journal-title":"J. Sci. Food Agric."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/j.nutres.2009.09.016","article-title":"In vitro bioaccessibility assessment as a prediction tool of nutritional efficiency","volume":"29","year":"2009","journal-title":"Nutr. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.jfca.2015.12.009","article-title":"Mineral composition and bioaccessibility in Sarcocornia ambigua using ICP-MS","volume":"47","author":"Bertin","year":"2016","journal-title":"J. Food Compost. Anal."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.talanta.2011.08.049","article-title":"Bioaccessibility of essential elements from white cheese, bread, fruit and vegetables","volume":"86","author":"Khouzam","year":"2011","journal-title":"Talanta"},{"key":"ref_51","first-page":"16","article-title":"Dietary oxalate and its intestinal absorption","volume":"9","author":"Holmes","year":"1995","journal-title":"Scanning Microsc."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1359\/jbmr.1999.14.5.661","article-title":"Strontium and bone","volume":"14","author":"Cabrera","year":"1999","journal-title":"J. Bone Miner. Res."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/3\/484\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:15:44Z","timestamp":1760134544000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/3\/484"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,7]]},"references-count":52,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["foods11030484"],"URL":"https:\/\/doi.org\/10.3390\/foods11030484","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,7]]}}}